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Wikipedia

Tempeh

Tempeh or tempe (/ˈtɛmp/; Javanese: ꦠꦺꦩ꧀ꦥꦺ, romanized: témpé, pronounced [tempe]) is a traditional Indonesian food made from fermented soybeans.[1] It is made by a natural culturing and controlled fermentation process that binds soybeans into a cake form.[2] A fungus, Rhizopus oligosporus or Rhizopus oryzae, is used in the fermentation process and is also known as tempeh starter.

Tempeh
Fresh tempeh
Alternative namesTempe
Place of originIndonesia[1]
Region or stateCentral Java,
East Java,
Special Region of Yogyakarta
Main ingredientsSoybeans
  •   Media: Tempeh
Tempeh being sold in a traditional market in Indonesia

It is especially popular on the island of Java, where it is a staple source of protein. Like tofu, tempeh is made from soybeans, but it is a whole-soybean product with different nutritional characteristics and textural qualities. Tempeh's fermentation process and its retention of the whole bean give it a higher content of protein, dietary fiber, and vitamins. It has a firm texture and an earthy flavor, which becomes more pronounced as it ages.[3][4]

Etymology

The term tempe is thought to be derived from the Old Javanese tumpi, a whitish food made of fried batter made from sago or rice flour which resembles rempeyek.[5] The historian Denys Lombard also suggests that it could be linked to a later term tape or tapai which means 'fermentation'.[6]

In the western world, tempeh is the most common spelling. This is done to prevent readers from incorrectly pronouncing the word as "temp". The first known usage of this spelling is in an 1896 German article. Other spellings, such as témpé, were also used, but tempeh has become the standard spelling in English since the 1960s.[7]

History

 
Tempeh being sold in Java, early 20th century

Tempeh originated in Indonesia, almost certainly in central or east Java[1] with an estimated discovery between a few centuries ago to a thousand years or more.[8]: 145 

The invention of tempeh cannot be separated from the origin of the fungus, which is the important part of the fermentation. This fungus consists of a mycelium that grows on teakwood and sea hibiscus leaves, which native Javanese people often used (and still do) as food wrappings. In fact, in traditional tempeh making, an usar (a mycelium-filled leaf) is used, instead of store-bought ragi.[9]

The type of soybean first used to make tempeh was the black soybean, which was a native plant.[10] This later changed with the importation of white/yellow soybeans and the rise of the tofu industry on the island.[11]

Debate over origins

Murdijati Gardjito, a food historian at Gadjah Mada University, argued that tempeh was made by native Javanese people, and that its preparation predates the introduction of Chinese-style tofu products.[12] Some ancient texts mention tempe dhele, old Javanese for 'native soybean tempeh'; dhele was used to refer to the native soybean variety. White soybeans that are used to make most tempe dhele today used to be called dhele putih ('white soybeans'), and were only available in Java centuries later. Mary Astuti, a food historian at Gadjah Mada University specializing in tempeh, argued that the native variety of soybean had been grown before the Chinese arrived in the region.[6]

Sri Tandjung noted that Javanese had been eating cooked (native black) soybeans since the 12th century. By the 16th or the 19th century, depending on which period of time the writer of Serat Centhini referred to, Javanese people had mastered the art of cooking with tempeh, where it was not only eaten as is, but converted into different types of dishes, showing a full understanding and mastery of the food product.[12]

Gardjito noted that Javanese noble families rarely wrote about tempeh in ancient texts because it had never been a part of royal cuisine, but rather a staple meal of the lower classes.[12]

Chinese Indonesian historian Ong Hok Ham suggests that tempeh might have been produced as a byproduct of tahu, the Indonesian word for tofu. He argued that the two food products are made of the same ingredient and that genetically speaking, soybeans are from China, though the specific variety was never mentioned. Food journalist Andreas Maryoto supported this idea, saying that tempeh might have been accidentally produced as the by-product of the tofu industry in Java in the 17th century, as discarded soybeans caught the spores of a whitish fungus that was found to be edible.[6]

However, tahu was (and is still) made of white soybeans (Glycine max, native to Japan and China), as opposed to the earliest version of tempe dhele that was made of native black soybeans (Glycine soja).[10][6]

Tahu (tofu) made its way to Kediri in the 13th century and was consumed by Mongolians who arrived in Java. Later, it was popular only among the rich (the complex production process and imported white soybeans led to its high price). Around the 17th or 19th century, tahu became available to everyone.[11]

Tempeh later began to be made with white soybeans, leading to the decreased use of its native black variety. Black soybeans have been replaced by other commodity plants since. The original version of tempe dhele has been forgotten as tahu has since become the common people's food, and dependence on imported white soybeans grows.[13]

Production

 
Making tempeh by wrapping boiled soybeans in banana leaves

Tempeh begins with whole soybeans, which are softened by soaking, and dehulled, then partly cooked. Specialty tempeh may be made from other types of beans, wheat, or may include a mixture of beans and whole grains.[14] Adding vinegar during soybeans soaking process had also been reported in tempeh industries and it had been found to influence the sensory nature of the final product.[15]

The principal step in making tempeh is the fermentation of soybeans which undergo inoculation with Rhizopus spp. molds, a type of filamentous fungus most widely used for the production of tempeh.[16] A fermentation starter containing the spores of fungus Rhizopus oligosporus or Rhizopus oryzae is mixed in.[17] The beans are spread into a thin layer and are allowed to ferment for 24 to 36 hours at a temperature around 30°C (86°F). The soybeans have to cool down to allow spore germination and abundant growth of mycelium. Later, the temperature of the beans will naturally rise and rapid mold growth happens for around 4 hours. As mold growth declines, the soybeans should be bound into a solid mass by the mycelium. In good tempeh, the beans are knitted together by a mat of white mycelium. Typically, tempeh is harvested after 48 hours of fermentation with its distinguishable whitish color, firm texture, and nutty flavor. Extended fermentation time results in an increase in pH and undesirable color darkening in the tempeh.[18]

 
 
 
 
 
Tempeh-making process using tempeh bag of sealed polyethylene pouch, soybeans prior to fermentation, after fermentation, and result

During the fermentation process, optimal time of fermentation, temperature, oxygen, humidity, and pH levels are required to encourage the growth of the Rhizopus mold, while discouraging the growth of undesired microorganisms.[19] The pH level should be kept around 3-5 by adding a mild acidulant such as vinegar, lactic acid, or acetic acid, thereby favoring mold growth and restricting the growth of spoilage microorganisms.[20] Oxygen is required for Rhizopus spp. growth, but should be maintained at low levels to prevent the production of undesired microorganisms. Under conditions of lower temperature, or higher ventilation, gray or black patches of spores may form on the surface—this is not harmful, and should not affect the flavor or quality of the tempeh.[21] This sporulation is normal on fully mature tempeh. A mild ammonia smell may accompany good tempeh as it ferments, but it should not be overpowering.

Traditional tempeh is often produced in Indonesia using Hibiscus tiliaceus leaves. The undersides of the leaves are covered in downy hairs (known technically as trichomes) to which the mold Rhizopus oligosporus can be found adhering in the wild. Soybeans are pressed into the leaf, and stored. Fermentation occurs resulting in tempeh.[14] In particular, the tempeh undergoes salt-free aerobic fermentation.[22]

Tempeh made with traditional inoculation methods are also more likely to include molds of other species including Rhizopus arrhizus and Rhizopus delemar which may outcompete Rhizopus oligosporus and become dominant. This resulted in white wooly appearance and more pleasant aroma compared with tempeh made with commercial starter containing only Rhizopus oligosporus. Famously these variant tempeh are found in Malang and Purwokerto in the 1960s, because Malang is located in a cool plateau, and tempeh made with Rhizopus oligosporus resulted in less compact and more alcoholic-smelling tempeh, while Rhizopus arrhizus required lower optimum temperature which made it more ideal and dominant. However the widespread use of commercial starter resulted in most tempeh in Java only contains Rhizopus oligosporus, with few traditionally made tempeh outside Java still contains Rhizopus arrhizus and Rhizopus delemar.[23]

Dry matter losses and yield

During the processing of soybeans to make tempeh there are inevitable losses of material due to the removal of the hulls and the leaching of soluble compounds during the soaking, washing and cooking stages. Hulls constitute about 8% of the dry beans and losses due to the leaching of soluble compounds equate to 12 - 17% of the dry beans. The oligosaccharides, stachyose, raffinose and sucrose, can constitute up to 50% of the soluble materials lost.[24][25] During the fermentation there is some further loss of material due to respiration by the mold and the oxidation of compounds to carbon dioxide and water. Reported losses of dry matter during the fermentation range from 2.1 to 10%.[24][25][26] Hence, the overall yield of tempeh is in the range of 72-78 g tempeh per 100 g soybeans on a dry matter basis. In practical terms, this means that 100 g dry soybeans (7-9% moisture content) will yield about 170 to 210 g fresh tempeh (61-64% moisture content).[24]

Determining quality

Once tempeh is produced, it is divided into three categories based on its quality: good, unfinished, and inedible. Good tempeh includes beans that are bound into a firm, compact cake by a dense, uniform, white mycelium, which should permeate the entire cake; the beans should be barely visible. The odor of good tempeh should be pleasant, clean, subtly sweet or resemble the aroma of mushrooms. The entire tempeh should lift as a single, cohesive cake without crumbling when shaken gently. Unfinished tempeh has beans that are bound together loosely by a sparse white mycelium, hence it crumbles easily. Unfinished tempeh should be incubated longer unless it has been incubated more than eight hours past the recommended time. If it has been incubated for enough time and still remains unfinished, it should be discarded. Inedible tempeh has beans with foul odor, resembling strong ammonia or alcohol, indicating the development of undesirable bacteria due to excess moisture or overheating. Inedible tempeh cake is wet, slimy, and mushy with a collapsed structure. Its color is tan to brown and mold develops in sparse patches.[20]

Packaging

 
Tempeh traditionally wrapped in banana leaf

Food grade wrapping paper and perforated polyethylene bags are the most suitable materials for packaging tempeh. They have demonstrated good retention of the quality of tempeh and extension of the shelf life of tempeh for three days compared to fresh tempeh. Appropriate packaging is important as it provides optimum oxygen supply and temperature for inoculation and fermentation to occur during processing.[27] Tempeh is a perishable food and must be wrapped and placed into the refrigerator or freezer immediately after incubation or other processing steps such as blanching. In the refrigerator or freezer, stacking of tempeh should be minimized to prevent overheating and the undesirable, gradual continuation of fermentation, both of which shorten the storage life of tempeh. Even under cold temperature, tempeh continues to respire and undergo slow decomposition from microorganisms and its natural enzymes. Therefore, tempeh should be well cooled for at least two to five hours in a cooler before they undergo further packaging.[20] Tempeh packaged in perforated polyethylene bags is usually repacked inside another labeled, non-perforated bag for distribution and sale, and for easier labeling. If the tempeh is only packaged in one perforated bag, the label must be directly attached to the perforated surface with the use of government food contact approved adhesive.[27] They are then bulk packed in cartons and returned to the refrigerator or freezer to await shipment.[20]

Nutrition

Tempeh
Nutritional value per 100 g (3.5 oz)
Energy803 kJ (192 kcal)
7.64 g
10.80 g
20.29 g
VitaminsQuantity
%DV
Thiamine (B1)
7%
0.078 mg
Riboflavin (B2)
30%
0.358 mg
Niacin (B3)
18%
2.640 mg
Vitamin B6
17%
0.215 mg
Folate (B9)
6%
24 μg
Vitamin B12
3%
0.08 μg
MineralsQuantity
%DV
Calcium
11%
111 mg
Iron
21%
2.7 mg
Magnesium
23%
81 mg
Manganese
62%
1.3 mg
Phosphorus
38%
266 mg
Potassium
9%
412 mg
Sodium
1%
9 mg
Zinc
12%
1.14 mg
Other constituentsQuantity
Water60 g

Full Link to USDA Database entry
Percentages are roughly approximated using US recommendations for adults.
Source: USDA FoodData Central

Tempeh is 60% water, 20% protein, 8% carbohydrates, and 11% fats (table). In a reference amount of 100 grams (3.5 oz), tempeh supplies 192 calories, and is a rich source (20% or more of the Daily Value, DV) of several B vitamins and dietary minerals, such as riboflavin (30% DV) and manganese (62% DV), respectively (table).

Effects of fermentation

The soy carbohydrates in tempeh become more digestible as a result of the fermentation process. In particular, the oligosaccharides associated with gas and indigestion are greatly reduced by the Rhizopus culture. In traditional tempeh-making shops, the starter culture often contains beneficial bacteria that produce vitamins such as B12[28][29] (though it is uncertain whether this B12 is always present and bioavailable).[30] In western countries, it is more common to use a pure culture containing only Rhizopus oligosporus, which makes little B12 and could be missing Citrobacter freundii and Klebsiella pneumoniae, which have been shown to produce significant levels of B12 analogs in tempeh when present.[31] Whether these analogs are true, bioavailable B12 has not been thoroughly studied yet.[32] The fermentation process also reduces the phytic acid in soy,[33] which in turn allows the body to absorb the minerals that soy provides.

Preparation

In the kitchen, tempeh is often simply prepared by cutting it into pieces, soaking in brine or a salty sauce, and then fried. In Java, tempeh is often traditionally prepared by cutting it into pieces, marinated in a mixture of ground garlic, coriander and turmeric, salt and water; then deep fried,[34] and often served with sambal ulek chili paste. Cooked tempeh can be eaten alone, or used in chili, stir fries, soups, salads, sandwiches, and stews. Tempeh's complex flavor has been described as nutty, meaty, and mushroom-like. It freezes well, and is now commonly available in many western supermarkets, as well as in ethnic markets and health food stores. Tempeh can be steamed, marinated, thinly sliced, blackened, or crumbled into sauces and stews.[35]

Tempeh performs well in a cheese grater, after which it may be used in place of ground beef (as in tacos). When thin-sliced and deep-fried in oil, tempeh obtains a crisp golden crust while maintaining a soft interior. Its sponge-like consistency makes it suitable for marinating. Dried tempeh (whether cooked or raw) is more portable and less perishable and may be used as a stew base. Sometimes when tempeh is diced and left, it will create white feathery fluff which bonds the cut—this is the Rhizopus mold still growing—this is normal and perfectly edible.[citation needed]

Types

 
Sliced tempe kedelai (soy tempeh)

The most common and widely known tempeh is made from fermented soybeans, called tempeh kedele or tempeh dele, made from controlled fermentation of soybeans. However, traditionally other ingredients such as ampas tahu (tofu dregs/okara), ampas kelapa (coconut dregs) and peanuts may be used in a fashion similar to the tempeh-making process, although perhaps using different fungi or attracting other microbes like kara benguk or kara pedhang, which can be toxic if not prepared correctly. A related product to tempeh is oncom, which is made from peanut press cake or soy dregs and is prevalent in Sundanese culture in West Java. There are two types of oncom: a bright red-orange kind with Neurospora sitophila, and a black one with the same fungi as tempeh uses.[36]

 
Sayur lodeh often have tempeh produced from advanced stages of fermentation mixed in for flavor

Tempeh can also be differentiated according to its degrees of maturity (i.e. the mycelium's growth/age). Tempe mondhol is a tempeh that is not fully fermented, that is, the mycelium has not fully covered the surface. The taste of the beans is more solid and profound. Sometimes tempeh is left to ferment further, creating more pungently varieties: tempe wayu (day-old tempeh), i.e. when the tempeh starts to age; tempe semangit (a few-days old tempeh), i.e. when the tempeh becomes yellowish, a bit slimy, and the smell becomes more potent;[37] and tempe bosok (lit. 'rotten tempeh'), when the mycelium has acquired a blackened coloration and the product has a putrid smell.

Some types of tempeh are made of ingredients that would be otherwise wasted if not used. According to traditional Javanese customs, wasting food is deemed as a sign of disrespect to Nature and other beings, and encourages efforts to use every part of an ingredient.

The wrappings used in tempeh making can contribute to its flavor and aroma. Though some prefer the traditional banana, waru or teak leaf, readily available plastic sheet wrappings have been increasingly widely used.

Tempe gembus

 
Tempe gembus

Soft and fluffy tempeh made from soy pulp or tofu dregs.[38][39] Tempe gembus usually can be found in traditional markets of Java, at a price lower than that of common soybean tempeh. It is made into a variety of dishes; for example it can be battered and/or fried, used in sayur lodeh, or tempe bacem. Tempe gembus is known by different names across Java; for example as tahu cokol or tahu susur in Temanggung.[38]

Tempe semangit

In Indonesia, ripe tempeh (two or more days old) is considered a delicacy. Names include tempe semangit ('stinky tempeh') in Java, hampir busuk ('almost rotten') tempeh or tempe kemarin ('yesterday tempeh'). Having a slightly pungent aroma, small amounts are used as a flavoring agent in traditional Javanese sayur lodeh vegetable stew and sambal tumpang.[37]

Tempe gódhóng

 
Tempe gódhóng jati (wrapped in teak leaf)

In Javanese, the term gódhóng means 'leaf'.[40] Traditionally tempeh is wrapped in organic banana leaf, gódhóng waru (Hibiscus tiliaceus leaf) or gódhóng jati (teak leaf).[41]

Tempe murni

Pure soybean cake, tempeh made in plastic wrap without any fillings or additives such as grated raw papaya. This was meant to create a more "hygienic and pure" tempeh free from any impurities or unwanted microbes.[citation needed]

Tempe menjes kacang

 
Menjes kacang

A specialty of Malang, the rough-textured tempeh menjes kacang is made from black soybeans mixed with other ingredients, such as peanut dregs, cassava fiber, and soybean meal. The process of making menjes kacang is quite similar to black oncom.[42]

Tempe bongkrèk

Tempe bongkrèk is a variety of tempeh from Central Java, notably Banyumas. It is prepared with coconut dregs. This type of tempeh has led to several cases of fatal food poisoning,[43] as it occasionally gets contaminated with the bacterium Burkholderia gladioli, and the unwanted organism produces toxins (bongkrek acid and toxoflavin) from the coconut, besides killing off the Rhizopus fungus due to the antibiotic activity of bongkrek acid.[39]

Fatalities from contaminated tempe bongkrèk were once common in the area where it was produced.[44] Thus, its sale is now prohibited by law; clandestine manufacture continues, however, due to the popular flavor. The problem of contamination is not encountered with bean and grain tempeh, which have a different composition of fatty acids that is not favorable for the growth of B. gladioli, but encourages growth of Rhizopus instead. When bean or grain tempeh has the proper color, texture and smell, it is a very strong indication the product is safe. Yellow tempe bongkrèk is always highly toxic due to toxoflavin, but tempe bongkrèk with a normal coloration may still contain lethal amounts of bongkrek acid.[45]

Oat tempeh

A form of tempeh based on barley and oats instead of soy was developed by scientists at the Swedish Department of Food Science in 2008. It can be produced in climatic regions where it is not possible to grow soybeans.[46]

Cooking methods and recipes

 
Sautéed tempeh with string green beans, an Indonesian dish

The simplest way to cook tempeh is by frying. It is both deep-fried and stir-fried. However, there are several cooking methods and recipe variations. Among others are:

Tempe goreng

Perhaps the simplest and most popular way to prepare tempeh in Indonesia. The tempeh is sliced and seasoned in a mixture of ground garlic, coriander seeds and salt, and then deep fried in palm oil.[47] The tempeh might be coated in batter prior to frying, or directly fried without any batter.

Tempe bacem

Tempe bacem is a traditional Javanese dish originating in Central Java. Bacem is a Javanese cooking method of braising in spices and palm sugar and boiling the food in a closed place until the water runs out.[48] The tempeh is first braised in a mixture of coconut water, palm sugar, and spices including coriander seeds, shallots, galangal, and bay leaves, and then briefly deep-fried. The result is a moist, sweet and spicy, dark-colored tempeh. Tofu may also be used, yielding tahu bacem.[49]

Tempe mendoan

 
Frying tempe mendoan
 
Cooked tempe garit/goreng (fried)

This variation is often found in Purwokerto. The word mendoan originates in the Banyumas regional dialect, and means 'flash-fried'. The tempeh is first dipped in spiced flour before quickly frying in very hot oil, resulting in a product that is cooked on the outside, but raw or only partially so on the inside. It has a limp, soft texture compared to the more common, crisp, fully fried tempeh.

Tempe kering

Also known as kering tempe (lit: 'dry tempeh'), or sambal goreng tempe if mixed with plenty of hot and spicy sambal chili pepper sauce. It is a crispy, sweet and spicy, fried tempeh.[50] The raw tempeh is cut into small sticks and thoroughly deep-fried until no longer moist, and then mixed with palm sugar, chili pepper or other spices, or with sweet soy sauce. Often it is mixed with separately fried peanuts and anchovies (ikan teri). This dry tempeh will keep for up to a month if cooked and stored properly.

Tempe orek or orak-arik tempe

This variation is almost identical to tempe kering, but is more soft and moist.[51] The sweet taste is due to generous addition of kecap manis (sweet soy sauce).[52]

Tumis tempe or oseng tempe

Stir-fried tempeh with vegetables such as green bean, basil, or onion, with spices.[53] Other recipes might add coconut milk for a milky-colored, and rather moist, stir-fried tempeh.

Tempe penyet

Fried tempeh mixed with sambal chili paste in a mortar and pestle. Usually served in addition to other penyet dishes, such as ayam penyet (chicken) or iga penyet (ribs).

Tempe satay

Tempeh skewered and grilled as satay.

Sate kere (Javanese for 'poor man's satay') from Solo in Central Java is made from fluffy tempe gembus.[54] Ground tempeh can also be made into a thick sauce, such as in sate ambal, a chicken satay from Kebumen, Central Java where tempeh flavored with chili and spices replaces the more common peanut sauce.[55]

Kripik tempe

Kripik tempe snack crackers; a thinly sliced tempeh, battered and deep fried until crispy. It is popular across Java, but notably produced in Bandung, West Java and Malang, East Java.[56]

Grilled tempeh

Grilled tempeh over charcoal or fire.[57]

Tempeh sandwich or tempeh burger

Fried, grilled or otherwise cooked tempeh patties, sandwiched between slices of bread or hamburger buns with salad, sauces or seasonings.[58]

Preservation

Freshly made, raw tempeh remains edible for a few days at room temperature. It is neither acidic nor does it contain significant amounts of alcohol. It, however, does possess stronger resistance to lipid peroxidation than unfermented soybeans due to its antioxidant contents.[59]

Cooked as tempe kering, the deep-fried and seasoned bits of tempeh can last for a month or more and still be good to consume, if cooked correctly and stored properly in an air-tight jar. The deep-frying process removes the moisture, preventing further fermentation and deterioration, thus prolonging its shelf life.

Antimicrobial agents

Rhizopus cultures responsible for the fermentation of tempeh from soybean produce natural, heat-stable antimicrobial agents against spoilage and disease-causing microorganisms, extending the shelf life of the fermented product through microbial antagonism. The mold is capable of inhibiting the growth of other fungi such as Aspergiluus flavus and Aspergillus parasiticus by interfering with the accumulation of aflatoxin (especially aflatoxin B1), the mycotoxin of greatest concern. R. oligosporus has also been reported to produce four to five antibacterial substances during fermentation process. It produces phenolic compounds against pathogenic bacteria such as Helicobacter pylori and an antibacterial protein has been identified with activities against Bacillus species (especially against Bacillus subtilis and Bacillus cereus[60]), Staphylococcus aureus, and Steptococcus cremoris.[16]

Non-refrigerated fresh tempeh

Tempeh can be sold and consumed fresh within 48 hours once removed from its incubator. It is commonly transported to the market in its incubation container (e.g. polyethylene bag, banana leaf wrapper, etc.) and placed in the shade. In areas with warmer climates, tempeh can be kept at room temperature for one to three days before it becomes overripe. In locations with more temperate temperatures, it can keep for one to four days but will usually need to be refrigerated to prevent spoilage.[20]

Refrigeration

Fresh refrigerated tempeh should be sealed in a labeled polyethylene bag and kept in temperatures below 4 °C (40 °F). It can be kept at this temperature for three to five days and sometimes, even as long as a week. Storage life could be extended to two or three weeks if the tempeh is blanched or steamed prior to refrigeration due to the inactivation of enzymes and destruction of bacteria.[20]

Freezing

Freezing is the preferred way to preserve tempeh due to its capability for wide distribution. Tempeh can be frozen whole or in slices, depending on preference. During the freezing process, whole tempeh is placed in its perforated wrapper whereas sliced tempeh is packaged in a labelled polyethylene bag prior to being sealed in an outer bag and then frozen immediately. This method will keep for months with only a small loss of texture and flavor.[20]

Blanching

Blanching tempeh by steaming or parboiling helps to extend the storage life by preventing bacterial growth, stopping mold growth and inactivating enzymes. Steaming appears to have a less negative effect than parboiling in terms of texture, flavor and nutritional value. Blanching is a great method for preserving tempeh prior to refrigeration, though not as beneficial for tempeh that is to be frozen.[20]

Dehydration

Air tray drying

Tempeh can be dried via the air tray drying method. Cubes of tempeh placed on steel, mesh bottom trays are dried by the circulating hot air dryer. After the product is finished, they can be cut into 2.5-centimetre (1-inch) squares at 90 °C (200 °F) for 90 to 120 minutes in order to reduce moisture content to 2–4%. When placed in moisture proof Pliofilm bags, the tempeh has a shelf life of several months at room temperature. Although this is a convenient method that produces a shelf stable product without requirement of refrigeration, the process of hot air drying can cause a significant loss of nutritional content such as the soluble solids and nitrogen protein content.[20]

Sun drying

This preservation method is most economical out of all methods. The tempeh can be blanched prior to dehydration to preserve flavor and prolong shelf life. Tempeh is exposed to internal solar dryer temperature of 80–90 °C (180–200 °F) in this method. A disadvantage of this method is that sunlight can destroy some of the vitamin B12 of tempeh.[20]

Freeze-drying

This method is the most expensive out of all dehydration methods but provides the advantage of long stable shelf life at room temperature and an excellent retention of soluble nutrients (nitrogen protein and other solids). The product undergoes quick freeze at 10 °C (50 °F) and is then dried at a moderate temperature inside a strong vacuum. Due to the expensive nature of the equipment, the final product price is higher than tempeh preserved through other methods.[20]

Spray-drying

As this method is traditionally used for small particles, it is used to produce tempeh powder for products such as soups, breads, tortillas, etc. However, this method can be expensive due to the bulky nature of the equipment.[20]

Deep-frying

This method produces ready to eat tempeh products. A culinary oil with a high smoke point, such as rapeseed, soy, safflower, peanut, or coconut oil, is heated to 180 °C (350 °F) in the deep fryer. The tempeh is deep-fried until golden brown and crisp, and then cooled quickly in a sterile environment to be sealed in Pliofilm bags and stored in a cool, dry place. The shelf life of this product lasts around a week but can be extended if the tempeh is sun dried or oven dried prior to deep frying.[20]

See also

References

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  2. ^ "Tempeh". Dictionary.com.
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tempeh, tempe, javanese, romanized, témpé, pronounced, tempe, traditional, indonesian, food, made, from, fermented, soybeans, made, natural, culturing, controlled, fermentation, process, that, binds, soybeans, into, cake, form, fungus, rhizopus, oligosporus, r. Tempeh or tempe ˈ t ɛ m p eɪ Javanese ꦠ ꦩ ꦥ romanized tempe pronounced tempe is a traditional Indonesian food made from fermented soybeans 1 It is made by a natural culturing and controlled fermentation process that binds soybeans into a cake form 2 A fungus Rhizopus oligosporus or Rhizopus oryzae is used in the fermentation process and is also known as tempeh starter TempehFresh tempehAlternative namesTempePlace of originIndonesia 1 Region or stateCentral Java East Java Special Region of YogyakartaMain ingredientsSoybeans Media Tempeh Tempeh being sold in a traditional market in Indonesia It is especially popular on the island of Java where it is a staple source of protein Like tofu tempeh is made from soybeans but it is a whole soybean product with different nutritional characteristics and textural qualities Tempeh s fermentation process and its retention of the whole bean give it a higher content of protein dietary fiber and vitamins It has a firm texture and an earthy flavor which becomes more pronounced as it ages 3 4 Contents 1 Etymology 2 History 2 1 Debate over origins 3 Production 3 1 Dry matter losses and yield 3 2 Determining quality 3 3 Packaging 4 Nutrition 4 1 Effects of fermentation 5 Preparation 6 Types 6 1 Tempe gembus 6 2 Tempe semangit 6 3 Tempe godhong 6 4 Tempe murni 6 5 Tempe menjes kacang 6 6 Tempe bongkrek 6 7 Oat tempeh 7 Cooking methods and recipes 7 1 Tempe goreng 7 2 Tempe bacem 7 3 Tempe mendoan 7 4 Tempe kering 7 5 Tempe orek or orak arik tempe 7 6 Tumis tempe or oseng tempe 7 7 Tempe penyet 7 8 Tempe satay 7 9 Kripik tempe 7 10 Grilled tempeh 7 11 Tempeh sandwich or tempeh burger 8 Preservation 8 1 Antimicrobial agents 8 2 Non refrigerated fresh tempeh 8 3 Refrigeration 8 4 Freezing 8 5 Blanching 8 6 Dehydration 8 6 1 Air tray drying 8 6 2 Sun drying 8 6 3 Freeze drying 8 6 4 Spray drying 8 6 5 Deep frying 9 See also 10 ReferencesEtymology EditThe term tempe is thought to be derived from the Old Javanese tumpi a whitish food made of fried batter made from sago or rice flour which resembles rempeyek 5 The historian Denys Lombard also suggests that it could be linked to a later term tape or tapai which means fermentation 6 In the western world tempeh is the most common spelling This is done to prevent readers from incorrectly pronouncing the word as temp The first known usage of this spelling is in an 1896 German article Other spellings such as tempe were also used but tempeh has become the standard spelling in English since the 1960s 7 History Edit Tempeh being sold in Java early 20th century Tempeh originated in Indonesia almost certainly in central or east Java 1 with an estimated discovery between a few centuries ago to a thousand years or more 8 145 The invention of tempeh cannot be separated from the origin of the fungus which is the important part of the fermentation This fungus consists of a mycelium that grows on teakwood and sea hibiscus leaves which native Javanese people often used and still do as food wrappings In fact in traditional tempeh making an usar a mycelium filled leaf is used instead of store bought ragi 9 The type of soybean first used to make tempeh was the black soybean which was a native plant 10 This later changed with the importation of white yellow soybeans and the rise of the tofu industry on the island 11 Debate over origins Edit Murdijati Gardjito a food historian at Gadjah Mada University argued that tempeh was made by native Javanese people and that its preparation predates the introduction of Chinese style tofu products 12 Some ancient texts mention tempe dhele old Javanese for native soybean tempeh dhele was used to refer to the native soybean variety White soybeans that are used to make most tempe dhele today used to be called dhele putih white soybeans and were only available in Java centuries later Mary Astuti a food historian at Gadjah Mada University specializing in tempeh argued that the native variety of soybean had been grown before the Chinese arrived in the region 6 Sri Tandjung noted that Javanese had been eating cooked native black soybeans since the 12th century By the 16th or the 19th century depending on which period of time the writer of Serat Centhini referred to Javanese people had mastered the art of cooking with tempeh where it was not only eaten as is but converted into different types of dishes showing a full understanding and mastery of the food product 12 Gardjito noted that Javanese noble families rarely wrote about tempeh in ancient texts because it had never been a part of royal cuisine but rather a staple meal of the lower classes 12 Chinese Indonesian historian Ong Hok Ham suggests that tempeh might have been produced as a byproduct of tahu the Indonesian word for tofu He argued that the two food products are made of the same ingredient and that genetically speaking soybeans are from China though the specific variety was never mentioned Food journalist Andreas Maryoto supported this idea saying that tempeh might have been accidentally produced as the by product of the tofu industry in Java in the 17th century as discarded soybeans caught the spores of a whitish fungus that was found to be edible 6 However tahu was and is still made of white soybeans Glycine max native to Japan and China as opposed to the earliest version of tempe dhele that was made of native black soybeans Glycine soja 10 6 Tahu tofu made its way to Kediri in the 13th century and was consumed by Mongolians who arrived in Java Later it was popular only among the rich the complex production process and imported white soybeans led to its high price Around the 17th or 19th century tahu became available to everyone 11 Tempeh later began to be made with white soybeans leading to the decreased use of its native black variety Black soybeans have been replaced by other commodity plants since The original version of tempe dhele has been forgotten as tahu has since become the common people s food and dependence on imported white soybeans grows 13 Production Edit Making tempeh by wrapping boiled soybeans in banana leaves Tempeh begins with whole soybeans which are softened by soaking and dehulled then partly cooked Specialty tempeh may be made from other types of beans wheat or may include a mixture of beans and whole grains 14 Adding vinegar during soybeans soaking process had also been reported in tempeh industries and it had been found to influence the sensory nature of the final product 15 The principal step in making tempeh is the fermentation of soybeans which undergo inoculation with Rhizopus spp molds a type of filamentous fungus most widely used for the production of tempeh 16 A fermentation starter containing the spores of fungus Rhizopus oligosporus or Rhizopus oryzae is mixed in 17 The beans are spread into a thin layer and are allowed to ferment for 24 to 36 hours at a temperature around 30 C 86 F The soybeans have to cool down to allow spore germination and abundant growth of mycelium Later the temperature of the beans will naturally rise and rapid mold growth happens for around 4 hours As mold growth declines the soybeans should be bound into a solid mass by the mycelium In good tempeh the beans are knitted together by a mat of white mycelium Typically tempeh is harvested after 48 hours of fermentation with its distinguishable whitish color firm texture and nutty flavor Extended fermentation time results in an increase in pH and undesirable color darkening in the tempeh 18 Tempeh making process using tempeh bag of sealed polyethylene pouch soybeans prior to fermentation after fermentation and result During the fermentation process optimal time of fermentation temperature oxygen humidity and pH levels are required to encourage the growth of the Rhizopus mold while discouraging the growth of undesired microorganisms 19 The pH level should be kept around 3 5 by adding a mild acidulant such as vinegar lactic acid or acetic acid thereby favoring mold growth and restricting the growth of spoilage microorganisms 20 Oxygen is required for Rhizopus spp growth but should be maintained at low levels to prevent the production of undesired microorganisms Under conditions of lower temperature or higher ventilation gray or black patches of spores may form on the surface this is not harmful and should not affect the flavor or quality of the tempeh 21 This sporulation is normal on fully mature tempeh A mild ammonia smell may accompany good tempeh as it ferments but it should not be overpowering Traditional tempeh is often produced in Indonesia using Hibiscus tiliaceus leaves The undersides of the leaves are covered in downy hairs known technically as trichomes to which the mold Rhizopus oligosporus can be found adhering in the wild Soybeans are pressed into the leaf and stored Fermentation occurs resulting in tempeh 14 In particular the tempeh undergoes salt free aerobic fermentation 22 Tempeh made with traditional inoculation methods are also more likely to include molds of other species including Rhizopus arrhizus and Rhizopus delemar which may outcompete Rhizopus oligosporus and become dominant This resulted in white wooly appearance and more pleasant aroma compared with tempeh made with commercial starter containing only Rhizopus oligosporus Famously these variant tempeh are found in Malang and Purwokerto in the 1960s because Malang is located in a cool plateau and tempeh made with Rhizopus oligosporus resulted in less compact and more alcoholic smelling tempeh while Rhizopus arrhizus required lower optimum temperature which made it more ideal and dominant However the widespread use of commercial starter resulted in most tempeh in Java only contains Rhizopus oligosporus with few traditionally made tempeh outside Java still contains Rhizopus arrhizus and Rhizopus delemar 23 Dry matter losses and yield Edit During the processing of soybeans to make tempeh there are inevitable losses of material due to the removal of the hulls and the leaching of soluble compounds during the soaking washing and cooking stages Hulls constitute about 8 of the dry beans and losses due to the leaching of soluble compounds equate to 12 17 of the dry beans The oligosaccharides stachyose raffinose and sucrose can constitute up to 50 of the soluble materials lost 24 25 During the fermentation there is some further loss of material due to respiration by the mold and the oxidation of compounds to carbon dioxide and water Reported losses of dry matter during the fermentation range from 2 1 to 10 24 25 26 Hence the overall yield of tempeh is in the range of 72 78 g tempeh per 100 g soybeans on a dry matter basis In practical terms this means that 100 g dry soybeans 7 9 moisture content will yield about 170 to 210 g fresh tempeh 61 64 moisture content 24 Determining quality Edit Once tempeh is produced it is divided into three categories based on its quality good unfinished and inedible Good tempeh includes beans that are bound into a firm compact cake by a dense uniform white mycelium which should permeate the entire cake the beans should be barely visible The odor of good tempeh should be pleasant clean subtly sweet or resemble the aroma of mushrooms The entire tempeh should lift as a single cohesive cake without crumbling when shaken gently Unfinished tempeh has beans that are bound together loosely by a sparse white mycelium hence it crumbles easily Unfinished tempeh should be incubated longer unless it has been incubated more than eight hours past the recommended time If it has been incubated for enough time and still remains unfinished it should be discarded Inedible tempeh has beans with foul odor resembling strong ammonia or alcohol indicating the development of undesirable bacteria due to excess moisture or overheating Inedible tempeh cake is wet slimy and mushy with a collapsed structure Its color is tan to brown and mold develops in sparse patches 20 Packaging Edit Tempeh traditionally wrapped in banana leaf Food grade wrapping paper and perforated polyethylene bags are the most suitable materials for packaging tempeh They have demonstrated good retention of the quality of tempeh and extension of the shelf life of tempeh for three days compared to fresh tempeh Appropriate packaging is important as it provides optimum oxygen supply and temperature for inoculation and fermentation to occur during processing 27 Tempeh is a perishable food and must be wrapped and placed into the refrigerator or freezer immediately after incubation or other processing steps such as blanching In the refrigerator or freezer stacking of tempeh should be minimized to prevent overheating and the undesirable gradual continuation of fermentation both of which shorten the storage life of tempeh Even under cold temperature tempeh continues to respire and undergo slow decomposition from microorganisms and its natural enzymes Therefore tempeh should be well cooled for at least two to five hours in a cooler before they undergo further packaging 20 Tempeh packaged in perforated polyethylene bags is usually repacked inside another labeled non perforated bag for distribution and sale and for easier labeling If the tempeh is only packaged in one perforated bag the label must be directly attached to the perforated surface with the use of government food contact approved adhesive 27 They are then bulk packed in cartons and returned to the refrigerator or freezer to await shipment 20 Nutrition EditTempehNutritional value per 100 g 3 5 oz Energy803 kJ 192 kcal Carbohydrates7 64 gFat10 80 gProtein20 29 gVitaminsQuantity DV Thiamine B1 7 0 078 mgRiboflavin B2 30 0 358 mgNiacin B3 18 2 640 mgVitamin B617 0 215 mgFolate B9 6 24 mgVitamin B123 0 08 mgMineralsQuantity DV Calcium11 111 mgIron21 2 7 mgMagnesium23 81 mgManganese62 1 3 mgPhosphorus38 266 mgPotassium9 412 mgSodium1 9 mgZinc12 1 14 mgOther constituentsQuantityWater60 gFull Link to USDA Database entryUnits mg micrograms mg milligrams IU International units Percentages are roughly approximated using US recommendations for adults Source USDA FoodData CentralTempeh is 60 water 20 protein 8 carbohydrates and 11 fats table In a reference amount of 100 grams 3 5 oz tempeh supplies 192 calories and is a rich source 20 or more of the Daily Value DV of several B vitamins and dietary minerals such as riboflavin 30 DV and manganese 62 DV respectively table Effects of fermentation Edit The soy carbohydrates in tempeh become more digestible as a result of the fermentation process In particular the oligosaccharides associated with gas and indigestion are greatly reduced by the Rhizopus culture In traditional tempeh making shops the starter culture often contains beneficial bacteria that produce vitamins such as B12 28 29 though it is uncertain whether this B12 is always present and bioavailable 30 In western countries it is more common to use a pure culture containing only Rhizopus oligosporus which makes little B12 and could be missing Citrobacter freundii and Klebsiella pneumoniae which have been shown to produce significant levels of B12 analogs in tempeh when present 31 Whether these analogs are true bioavailable B12 has not been thoroughly studied yet 32 The fermentation process also reduces the phytic acid in soy 33 which in turn allows the body to absorb the minerals that soy provides Preparation Edit Tempeh burger In the kitchen tempeh is often simply prepared by cutting it into pieces soaking in brine or a salty sauce and then fried In Java tempeh is often traditionally prepared by cutting it into pieces marinated in a mixture of ground garlic coriander and turmeric salt and water then deep fried 34 and often served with sambal ulek chili paste Cooked tempeh can be eaten alone or used in chili stir fries soups salads sandwiches and stews Tempeh s complex flavor has been described as nutty meaty and mushroom like It freezes well and is now commonly available in many western supermarkets as well as in ethnic markets and health food stores Tempeh can be steamed marinated thinly sliced blackened or crumbled into sauces and stews 35 Tempeh performs well in a cheese grater after which it may be used in place of ground beef as in tacos When thin sliced and deep fried in oil tempeh obtains a crisp golden crust while maintaining a soft interior Its sponge like consistency makes it suitable for marinating Dried tempeh whether cooked or raw is more portable and less perishable and may be used as a stew base Sometimes when tempeh is diced and left it will create white feathery fluff which bonds the cut this is the Rhizopus mold still growing this is normal and perfectly edible citation needed Types Edit Sliced tempe kedelai soy tempeh The most common and widely known tempeh is made from fermented soybeans called tempeh kedele or tempeh dele made from controlled fermentation of soybeans However traditionally other ingredients such as ampas tahu tofu dregs okara ampas kelapa coconut dregs and peanuts may be used in a fashion similar to the tempeh making process although perhaps using different fungi or attracting other microbes like kara benguk or kara pedhang which can be toxic if not prepared correctly A related product to tempeh is oncom which is made from peanut press cake or soy dregs and is prevalent in Sundanese culture in West Java There are two types of oncom a bright red orange kind with Neurospora sitophila and a black one with the same fungi as tempeh uses 36 Sayur lodeh often have tempeh produced from advanced stages of fermentation mixed in for flavor Tempeh can also be differentiated according to its degrees of maturity i e the mycelium s growth age Tempe mondhol is a tempeh that is not fully fermented that is the mycelium has not fully covered the surface The taste of the beans is more solid and profound Sometimes tempeh is left to ferment further creating more pungently varieties tempe wayu day old tempeh i e when the tempeh starts to age tempe semangit a few days old tempeh i e when the tempeh becomes yellowish a bit slimy and the smell becomes more potent 37 and tempe bosok lit rotten tempeh when the mycelium has acquired a blackened coloration and the product has a putrid smell Some types of tempeh are made of ingredients that would be otherwise wasted if not used According to traditional Javanese customs wasting food is deemed as a sign of disrespect to Nature and other beings and encourages efforts to use every part of an ingredient The wrappings used in tempeh making can contribute to its flavor and aroma Though some prefer the traditional banana waru or teak leaf readily available plastic sheet wrappings have been increasingly widely used Tempe gembus Edit Tempe gembus Soft and fluffy tempeh made from soy pulp or tofu dregs 38 39 Tempe gembus usually can be found in traditional markets of Java at a price lower than that of common soybean tempeh It is made into a variety of dishes for example it can be battered and or fried used in sayur lodeh or tempe bacem Tempe gembus is known by different names across Java for example as tahu cokol or tahu susur in Temanggung 38 Tempe semangit Edit In Indonesia ripe tempeh two or more days old is considered a delicacy Names include tempe semangit stinky tempeh in Java hampir busuk almost rotten tempeh or tempe kemarin yesterday tempeh Having a slightly pungent aroma small amounts are used as a flavoring agent in traditional Javanese sayur lodeh vegetable stew and sambal tumpang 37 Tempe godhong Edit Tempe godhong jati wrapped in teak leaf In Javanese the term godhong means leaf 40 Traditionally tempeh is wrapped in organic banana leaf godhong waru Hibiscus tiliaceus leaf or godhong jati teak leaf 41 Tempe murni Edit Pure soybean cake tempeh made in plastic wrap without any fillings or additives such as grated raw papaya This was meant to create a more hygienic and pure tempeh free from any impurities or unwanted microbes citation needed Tempe menjes kacang Edit Menjes kacang A specialty of Malang the rough textured tempeh menjes kacang is made from black soybeans mixed with other ingredients such as peanut dregs cassava fiber and soybean meal The process of making menjes kacang is quite similar to black oncom 42 Tempe bongkrek Edit Tempe bongkrek is a variety of tempeh from Central Java notably Banyumas It is prepared with coconut dregs This type of tempeh has led to several cases of fatal food poisoning 43 as it occasionally gets contaminated with the bacterium Burkholderia gladioli and the unwanted organism produces toxins bongkrek acid and toxoflavin from the coconut besides killing off the Rhizopus fungus due to the antibiotic activity of bongkrek acid 39 Fatalities from contaminated tempe bongkrek were once common in the area where it was produced 44 Thus its sale is now prohibited by law clandestine manufacture continues however due to the popular flavor The problem of contamination is not encountered with bean and grain tempeh which have a different composition of fatty acids that is not favorable for the growth of B gladioli but encourages growth of Rhizopus instead When bean or grain tempeh has the proper color texture and smell it is a very strong indication the product is safe Yellow tempe bongkrek is always highly toxic due to toxoflavin but tempe bongkrek with a normal coloration may still contain lethal amounts of bongkrek acid 45 Oat tempeh Edit A form of tempeh based on barley and oats instead of soy was developed by scientists at the Swedish Department of Food Science in 2008 It can be produced in climatic regions where it is not possible to grow soybeans 46 Cooking methods and recipes Edit Sauteed tempeh with string green beans an Indonesian dish The simplest way to cook tempeh is by frying It is both deep fried and stir fried However there are several cooking methods and recipe variations Among others are Tempe goreng Edit Perhaps the simplest and most popular way to prepare tempeh in Indonesia The tempeh is sliced and seasoned in a mixture of ground garlic coriander seeds and salt and then deep fried in palm oil 47 The tempeh might be coated in batter prior to frying or directly fried without any batter Tempe bacem Edit Tempe bacem is a traditional Javanese dish originating in Central Java Bacem is a Javanese cooking method of braising in spices and palm sugar and boiling the food in a closed place until the water runs out 48 The tempeh is first braised in a mixture of coconut water palm sugar and spices including coriander seeds shallots galangal and bay leaves and then briefly deep fried The result is a moist sweet and spicy dark colored tempeh Tofu may also be used yielding tahu bacem 49 Tempe mendoan Edit Frying tempe mendoan Cooked tempe garit goreng fried This variation is often found in Purwokerto The word mendoan originates in the Banyumas regional dialect and means flash fried The tempeh is first dipped in spiced flour before quickly frying in very hot oil resulting in a product that is cooked on the outside but raw or only partially so on the inside It has a limp soft texture compared to the more common crisp fully fried tempeh Wikibooks Cookbook has a recipe module on Tempe Mendoan Tempe kering Edit Also known as kering tempe lit dry tempeh or sambal goreng tempe if mixed with plenty of hot and spicy sambal chili pepper sauce It is a crispy sweet and spicy fried tempeh 50 The raw tempeh is cut into small sticks and thoroughly deep fried until no longer moist and then mixed with palm sugar chili pepper or other spices or with sweet soy sauce Often it is mixed with separately fried peanuts and anchovies ikan teri This dry tempeh will keep for up to a month if cooked and stored properly Tempe orek or orak arik tempe Edit This variation is almost identical to tempe kering but is more soft and moist 51 The sweet taste is due to generous addition of kecap manis sweet soy sauce 52 Tumis tempe or oseng tempe Edit Stir fried tempeh with vegetables such as green bean basil or onion with spices 53 Other recipes might add coconut milk for a milky colored and rather moist stir fried tempeh Tempe penyet Edit Fried tempeh mixed with sambal chili paste in a mortar and pestle Usually served in addition to other penyet dishes such as ayam penyet chicken or iga penyet ribs Tempe satay Edit Tempeh skewered and grilled as satay Sate kere Javanese for poor man s satay from Solo in Central Java is made from fluffy tempe gembus 54 Ground tempeh can also be made into a thick sauce such as in sate ambal a chicken satay from Kebumen Central Java where tempeh flavored with chili and spices replaces the more common peanut sauce 55 Kripik tempe Edit Kripik tempe snack crackers a thinly sliced tempeh battered and deep fried until crispy It is popular across Java but notably produced in Bandung West Java and Malang East Java 56 Grilled tempeh Edit Grilled tempeh over charcoal or fire 57 Tempeh sandwich or tempeh burger Edit Fried grilled or otherwise cooked tempeh patties sandwiched between slices of bread or hamburger buns with salad sauces or seasonings 58 Tempeh dishes The common tempeh goreng un battered in Indonesia Fried tempeh battered sold at a food court in Singapore Tempe bacem Tempe mendoan Kering tempe or sambal goreng tempe Tempe orek or orak arik tempe Tempe penyet Crispy kripik tempeh as a snack Fried menjes kacang Grilled tempeh Tempeh sandwichPreservation EditFreshly made raw tempeh remains edible for a few days at room temperature It is neither acidic nor does it contain significant amounts of alcohol It however does possess stronger resistance to lipid peroxidation than unfermented soybeans due to its antioxidant contents 59 Cooked as tempe kering the deep fried and seasoned bits of tempeh can last for a month or more and still be good to consume if cooked correctly and stored properly in an air tight jar The deep frying process removes the moisture preventing further fermentation and deterioration thus prolonging its shelf life Antimicrobial agents Edit Rhizopus cultures responsible for the fermentation of tempeh from soybean produce natural heat stable antimicrobial agents against spoilage and disease causing microorganisms extending the shelf life of the fermented product through microbial antagonism The mold is capable of inhibiting the growth of other fungi such as Aspergiluus flavus and Aspergillus parasiticus by interfering with the accumulation of aflatoxin especially aflatoxin B1 the mycotoxin of greatest concern R oligosporus has also been reported to produce four to five antibacterial substances during fermentation process It produces phenolic compounds against pathogenic bacteria such as Helicobacter pylori and an antibacterial protein has been identified with activities against Bacillus species especially against Bacillus subtilis and Bacillus cereus 60 Staphylococcus aureus and Steptococcus cremoris 16 Non refrigerated fresh tempeh Edit Tempeh can be sold and consumed fresh within 48 hours once removed from its incubator It is commonly transported to the market in its incubation container e g polyethylene bag banana leaf wrapper etc and placed in the shade In areas with warmer climates tempeh can be kept at room temperature for one to three days before it becomes overripe In locations with more temperate temperatures it can keep for one to four days but will usually need to be refrigerated to prevent spoilage 20 Refrigeration Edit Fresh refrigerated tempeh should be sealed in a labeled polyethylene bag and kept in temperatures below 4 C 40 F It can be kept at this temperature for three to five days and sometimes even as long as a week Storage life could be extended to two or three weeks if the tempeh is blanched or steamed prior to refrigeration due to the inactivation of enzymes and destruction of bacteria 20 Freezing Edit Freezing is the preferred way to preserve tempeh due to its capability for wide distribution Tempeh can be frozen whole or in slices depending on preference During the freezing process whole tempeh is placed in its perforated wrapper whereas sliced tempeh is packaged in a labelled polyethylene bag prior to being sealed in an outer bag and then frozen immediately This method will keep for months with only a small loss of texture and flavor 20 Blanching Edit Blanching tempeh by steaming or parboiling helps to extend the storage life by preventing bacterial growth stopping mold growth and inactivating enzymes Steaming appears to have a less negative effect than parboiling in terms of texture flavor and nutritional value Blanching is a great method for preserving tempeh prior to refrigeration though not as beneficial for tempeh that is to be frozen 20 Dehydration Edit Air tray drying Edit Tempeh can be dried via the air tray drying method Cubes of tempeh placed on steel mesh bottom trays are dried by the circulating hot air dryer After the product is finished they can be cut into 2 5 centimetre 1 inch squares at 90 C 200 F for 90 to 120 minutes in order to reduce moisture content to 2 4 When placed in moisture proof Pliofilm bags the tempeh has a shelf life of several months at room temperature Although this is a convenient method that produces a shelf stable product without requirement of refrigeration the process of hot air drying can cause a significant loss of nutritional content such as the soluble solids and nitrogen protein content 20 Sun drying Edit This preservation method is most economical out of all methods The tempeh can be blanched prior to dehydration to preserve flavor and prolong shelf life Tempeh is exposed to internal solar dryer temperature of 80 90 C 180 200 F in this method A disadvantage of this method is that sunlight can destroy some of the vitamin B12 of tempeh 20 Freeze drying Edit This method is the most expensive out of all dehydration methods but provides the advantage of long stable shelf life at room temperature and an excellent retention of soluble nutrients nitrogen protein and other solids The product undergoes quick freeze at 10 C 50 F and is then dried at a moderate temperature inside a strong vacuum Due to the expensive nature of the equipment the final product price is higher than tempeh preserved through other methods 20 Spray drying Edit As this method is traditionally used for small particles it is used to produce tempeh powder for products such as soups breads tortillas etc However this method can be expensive due to the bulky nature of the equipment 20 Deep frying Edit This method produces ready to eat tempeh products A culinary oil with a high smoke point such as rapeseed soy safflower peanut or coconut oil is heated to 180 C 350 F in the deep fryer The tempeh is deep fried until golden brown and crisp and then cooled quickly in a sterile environment to be sealed in Pliofilm bags and stored in a cool dry place The shelf life of this product lasts around a week but can be extended if the tempeh is sun dried or oven dried prior to deep frying 20 See also Edit Food portal Indonesia portalList of fermented soy products List of meat substitutes List of soy based foods Miso Nattō Oncom Tapai Food portal VeganismReferences Edit a b c William Shurtleff Akiko Aoyagi History of Tempeh www soyinfocenter com p 1 Retrieved 2018 09 16 Tempeh Dictionary com Bennett Beverly Lynn Sammartano Ray 2008 The Complete Idiot s Guide to Vegan Cooking Penguin p 17 ISBN 9781592577705 Retrieved 6 May 2011 Dragonwagon Crescent Gourley Robbin 2002 Passionate Vegetarian Workman Publishing p 639 ISBN 9781563057113 Retrieved 6 May 2011 Resep Peyek tumpi teri medan oleh Siti bundae arfa shaka Cookpad in Indonesian Retrieved 2018 08 03 a b c d Hendri F Isnaeni 9 July 2014 Sejarah Tempe in Indonesian Historia Retrieved 30 May 2015 History of Tempeh page 1 www soyinfocenter com Retrieved 2020 10 25 Shurtleff William Aoyagi Akiko 1979 The Book of Tempeh Soyinfo Center ISBN 9780060140090 Tamam Mh Badrut 2017 06 13 Jenis dan Mekanisme Fermentasi Mikroorganisme Generasi Biologi Retrieved 2021 02 23 a b Sejarah Kedelai Manfaat Kedelai Dan Serat Dalam Kedelai Distributor bahan pakan ternak hijauan bungkil kedelai meat bone meal DDGS kedelai import Retrieved 2021 02 23 a b Sejarah Tahu Tahu Sejarah Historia Majalah Sejarah Populer Pertama di Indonesia in Indonesian 2014 01 10 Retrieved 2021 02 23 a b c Media Kompas Cyber 12 September 2020 Sejarah Tempe Makanan Asli Indonesia yang Mendunia KOMPAS com in Indonesian Retrieved 2021 02 23 Agricultural Commodities Indonesia Highly Dependent on Soybean Imports Indonesia Investments www indonesia investments com Retrieved 2021 02 23 a b Shurtleff William Aoyagi Akiko 1979 The Book of Tempeh Soyinfo Center Harper and Row ISBN 9780060140090 Archived from the original PDF on 2013 08 19 Dahlan Hadi Akbar Nambu Yosuke Putri Sastia Prama Fukusaki Eiichiro January 2022 Effects of Soaking Tempe in Vinegar on Metabolome and Sensory Profiles Metabolites 12 1 30 doi 10 3390 metabo12010030 ISSN 2218 1989 PMC 8781261 PMID 35050152 a b Dinesh Babu P Vidhyalakshmi R 2018 08 08 A Low Cost Nutritious Food Tempeh A Review a href Template Cite journal html title Template Cite journal cite journal a Cite journal requires journal help What is tempeh starter Tempeh info Muzdalifah D Athaillah Z A Nugrahani W Devi A F 2017 Colour and pH changes of tempe during extended fermentation International Symposium on Applied Chemistry AIP Conference Proceedings Vol 1803 p 020036 Bibcode 2017AIPC 1803b0036M doi 10 1063 1 4973163 Nout M J R Kiers J L April 2005 Tempe fermentation innovation and functionality update into the third millenium Journal of Applied Microbiology 98 4 789 805 doi 10 1111 j 1365 2672 2004 02471 x ISSN 1364 5072 PMID 15752324 S2CID 19259459 a b c d e f g h i j k l m Shurtleff William 1986 Tempeh production a craft and technical manual Aoyagi Akiko Shurtleff William 1941 Soyfoods Center Lafayette Calif 2nd ed Lafayette CA Soyfoods Center ISBN 978 0933332232 OCLC 13002817 How to Make and Cook Tempeh Mother Earth News September October 1977 Retrieved 3 January 2013 Watanabe N Fujimoto K Aoki H 2007 Antioxidant activities of the water soluble fraction in tempeh like fermented soybean GABA tempeh International Journal of Food Sciences and Nutrition 58 8 577 587 doi 10 1080 09637480701343846 PMID 17852485 S2CID 45727148 Sjamsuridzal Wellyzar Khasanah Mangunatun Febriani Rela Vebliza Yura Oetari Ariyanti Santoso Iman Gandjar Indrawati 2021 12 14 The effect of the use of commercial tempeh starter on the diversity of Rhizopus tempeh in Indonesia Scientific Reports 11 1 23932 Bibcode 2021NatSR 1123932S doi 10 1038 s41598 021 03308 6 ISSN 2045 2322 PMC 8671487 PMID 34907227 a b c Steinkraus K H 1996 Handbook of Indigenous Fermented Foods New York Marcell Dekker pp 7 110 ISBN 0 8247 9352 8 a b Owens J D Astuti M Kuswanto K R 2015 Tempe and related products In Owens J D ed Indigenous Fermented Foods of Southeast Asia Boca CRC Press pp 1 108 ISBN 978 1 4398 4480 9 Sparringa R A Owens J D 1999 Protein utilization during soybean tempe fermentation Journal of Agricultural and Food Chemistry 47 10 4375 4378 doi 10 1021 jf981279u PMID 10552819 a b Santhirasegaram Vicknesha George Dominic Soloman Anthony Kelvin Kiran Singh Hasvinder Kaur Baldev Saruan Nadiah Mohd Razali Zuliana Somasundram Chandran December 2016 Effects of Soybean Processing and Packaging on the Quality of Commonly Consumed Local Delicacy Tempe Journal of Food Quality 39 6 675 684 doi 10 1111 jfq 12252 ISSN 0146 9428 Liem IT Steinkraus KH Cronk TC December 1977 Production of vitamin B 12 in tempeh a fermented soybean food Appl Environ Microbiol 34 6 773 6 Bibcode 1977ApEnM 34 773L doi 10 1128 AEM 34 6 773 776 1977 PMC 242746 PMID 563702 Truesdell Delores D Green Nancy R Acosta Phyllis B 1987 Vitamin B12 Activity in Miso and Tempeh Journal of Food Science 52 2 493 494 doi 10 1111 j 1365 2621 1987 tb06650 x Archived from the original on 2013 01 05 Allison A Yates 2001 National Nutrition and Public Health Policies Issues Related to Bio availability of Nutrients When Developing Dietary Reference Intakes from January 2000 conference Bio availability of Nutrients and Other Bio active Components from Dietary Supplements The Journal of Nutrition 131 4 1331S 1334S doi 10 1093 jn 131 4 1331S PMID 11285348 Keuth S Bisping B May 1994 Vitamin B12 production by Citrobacter freundii and Klebsiella pneumoniae during tempeh fermentation Applied and Environmental Microbiology 60 5 1495 9 doi 10 1128 AEM 60 5 1495 1499 1994 PMC 201508 PMID 8017933 Vitamin B12 Are You Getting It Vegan Health Archived from the original on 2008 11 03 Amanda Rose Soy and Phytic Acid Stick with Fermented Tempeh and Miso Reducing Phytic Acid in Your Food A visual analysis of the research on home kitchen remedies for phytic acid Rebuild Market Archived from the original on 6 January 2012 Retrieved 29 December 2011 Najam Pawon Mommy Resep Tempe Goreng Garit oleh pawon mommy najam Cookpad in Indonesian Retrieved 2019 10 17 Five Ways to Prepare Tempeh Kitchn Retrieved 2018 01 19 Wijaya Kirana 2014 03 01 Lauk Tempe Tahu amp Oncom in Indonesian DeMedia ISBN 9789790822061 a b Penulis Wahyu Adityo Prodjo 23 July 2016 Olahan Tempe Busuk Murah Meriah nan Menggugah Selera KOMPAS com in Indonesian Retrieved 2019 10 17 a b Safira Maya Tempe Gembus yang Empuk Menthul menthul Kayak Kasur detikfood in Indonesian Retrieved 2018 01 19 a b Mustinda Lusiana Apa Benar Tempe Gembus dan Tempe Bongkrek Nutrisinya Rendah detikfood in Indonesian Retrieved 2018 01 18 Arti kata godhong godhong dalam kamus Jawa Indonesia Terjemahan dari bahasa Jawa ke bahasa Indonesia Kamus lengkap online semua bahasa kamuslengkap com in Indonesian Retrieved 2018 01 19 Mikrobiologi Tempe Daun Waru Scribd in Indonesian Retrieved 2018 01 19 Maharrani Anindhita 2016 08 26 Menjes tempe khas dari Malang Beritagar in Indonesian Retrieved 2018 01 19 Shurtleff William Aoyagi Akiko 2010 History of Soybeans and Soyfoods in Southeast Asia 13th Century To 2010 Extensively Annotated Bibliography and Sourcebook Soyinfo Center ISBN 9781928914303 Liputan6 com 25 September 2003 Bahaya Tempe Bongkrek Kurang Sosialisasi liputan6 com in Indonesian Retrieved 2018 01 18 Setiarto Raden Haryo Bimo Waspadai Toksoflavin dan Asam Bongkrek Yang Dihasilkan Bakteri Pseudomonas in Indonesian Retrieved 2018 01 18 New Vegetarian Food With Several Health Benefits ScienceDaily May 30 2008 Retrieved 20 January 2018 Fried Tempeh amp Tofu Tahu Tempe Goreng www lestariweb com Archived from the original on 2018 01 20 Retrieved 2018 01 19 Indonesia Aplikasi arti bacem adalah dalam Kamus Besar Bahasa Indonesia KBBI Online aplikasi indonesia com in Indonesian Retrieved 2020 12 05 Tahu dan Tempe Bacem Braised Spiced Tofu and Temphe What To Cook Today 2017 07 21 Retrieved 2018 01 19 Crispy And Spicy Fried Tempe Kering Tempe Indonesian Recipe 2013 08 07 Retrieved 2018 01 19 Tempe Orek Recipe Another Classical of Cooking Tempeh indonesianfoods recipes blogspot co id Archived from the original on 2018 01 20 Retrieved 2018 01 19 Orek Tempe Retrieved 2018 01 19 4 971 resep oseng tempe enak dan sederhana Cookpad in Indonesian Retrieved 2018 01 19 11 resep sate tempe gembus enak dan sederhana Cookpad in Indonesian Retrieved 2018 01 19 Recipe for Sate Ambal chickensatay org Retrieved 2018 01 19 Kampung Sanan Sentra Industri Tempe yang Hasilkan Rp 1 M per Hari kumparan in Indonesian Retrieved 2022 10 28 How to Master Grilled Tempeh Organic Authority Organic Authority 2011 08 24 Retrieved 2018 01 20 Tempeh Vegan Club Sandwiches Recipe Love and Lemons Love and Lemons 2017 04 06 Retrieved 2018 01 20 Umm al Qura University Mecca Saudi Arabia Handbook of Fermented Functional Foods Archived 2015 07 14 at the Wayback Machine Roubos van den Hil P J Dalmas E Nout M J R Abee T 2010 Soya bean tempe extracts show antibacterial activity against Bacillus cereus cells and spores Journal of Applied Microbiology 109 1 137 145 doi 10 1111 j 1365 2672 2009 04637 x ISSN 1364 5072 PMID 20002864 S2CID 44995408 Retrieved from https en wikipedia org w index php title Tempeh amp oldid 1148905497, wikipedia, wiki, book, books, library,

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