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Roger Y. Tsien

October 21, 2023

Roger Yonchien Tsien (pronounced /tʃɛn/, "CHEN"; February 1, 1952 – August 24, 2016) was an American biochemist. He was a professor of chemistry and biochemistry at the University of California, San Diego[7] and was awarded the Nobel Prize in Chemistry in 2008 for his discovery and development of the green fluorescent protein, in collaboration with organic chemist Osamu Shimomura and neurobiologist Martin Chalfie.[8][9][10] Tsien was also a pioneer of calcium imaging.[9][11]

Roger Tsien
Tsien in 2008
Born
Roger Yonchien Tsien

(1952-02-01)February 1, 1952
New York City, U.S.
DiedAugust 24, 2016(2016-08-24) (aged 64)
Eugene, Oregon, U.S.[5]
NationalityAmerican
CitizenshipUnited States
Education
(PhD)
Known for
SpouseWendy Globe[5]
Awards
Scientific career
FieldsBiochemistry
Institutions
ThesisThe design and use of organic chemical tools in cellular physiology (1976)
Doctoral advisorRichard Adrian[4]
Chinese name
Traditional Chinese錢永健
Simplified Chinese钱永健
Transcriptions
Standard Mandarin
Hanyu PinyinQián Yǒngjiàn
Wade–GilesCh'ien Yung-chien
Websitewww.tsienlab.ucsd.edu

Early life Edit

 
Paul Krugman, Roger Tsien, Martin Chalfie, Osamu Shimomura, Makoto Kobayashi and Toshihide Masukawa, Nobel Prize Laureates 2008, at a press conference at the Swedish Academy of Science in Stockholm.

Tsien was born to a Chinese American family in New York, in 1952.[12] He grew up in Livingston, New Jersey[12] and attended Livingston High School.[13] Tsien traces his family ancestry to Hangzhou, China. His father Hsue-Chu Tsien, an MIT and Shanghai Jiao Tong University alumnus, was a mechanical engineer and had excelled academically, graduating at the top of his university class.

Tsien suffered from asthma as a child, and as a result, he was often indoors. He spent hours conducting chemistry experiments in his basement laboratory. When he was 16, he won first prize in the nationwide Westinghouse Talent Search with a project investigating how metals bind to thiocyanate.[12]

Education Edit

Tsien attended Harvard College on a National Merit Scholarship, where he was elected to Phi Beta Kappa as a junior.[14] He graduated summa cum laude with a Bachelor of Arts in chemistry and physics in 1972.[15] According to his freshman-year roommate, economist and Iowa politician Herman Quirmbach, "It's probably not an exaggeration to say he's the smartest person I ever met ... [a]nd I have met a lot of brilliant people."[16]

After completing his bachelor's degree, Tsien joined the Physiological Laboratory at the University of Cambridge in Cambridge, England with the aid of a Marshall Scholarship, and resided at Churchill College, Cambridge.[17] He received his Ph.D. in physiology in 1977 for research on The Design and Use of Organic Chemical Tools in Cellular Physiology [4] formally supervised by Richard Adrian in the department of physiology and assisted by Andy Holmes, Gerry Smith and Jeremy Sanders in the department of chemistry.[17]

Research and career Edit

Following his Ph.D., Tsien was a research fellow at Gonville and Caius College, Cambridge from 1977 to 1981.[17] He was appointed to the faculty at the University of California, Berkeley, from 1982 to 1989.[18] Beginning in 1989, he worked at the University of California, San Diego, as professor of pharmacology and professor of chemistry and biochemistry,[7] and as an investigator of the Howard Hughes Medical Institute.[19][20]

Tsien contributed to the fields of cell biology and neurobiology by discovering genetically programmable fluorescent tags, thereby allowing scientists to watch the behavior of molecules in living cells in real time. He also developed fluorescent indicators of calcium ions and other ions important in biological processes.

 
GFP Movie showing entire structure and zoom in to fluorescent chromophore. Movie created by Erik A. Rodriguez with UCSF Chimera from PDB: 1EMA in memory of Roger Y. Tsien for the Birch Aquarium.

In 2004, Tsien was awarded the Wolf Prize in Medicine "for his seminal contribution to the design and biological application of novel fluorescent and photolabile molecules to analyze and perturb cell signal transduction."[21]

In 2008, Tsien shared the Nobel Prize in Chemistry with Osamu Shimomura and Martin Chalfie for "the green fluorescent protein: discovery, expression and development."[8][22]

Fluorescent proteins Edit

The multicolored fluorescent proteins developed in Tsien's lab are used by scientists to track where and when certain genes are expressed in cells or in whole organisms. Typically, the gene coding for a protein of interest is fused with the gene for a fluorescent protein, which causes the protein of interest to glow inside the cell when the cell is irradiated with a suitable wavelength of light and allows microscopists to track its location in real time. This is such a popular technique that it has added a new dimension to the fields of molecular biology, cell biology, and biochemistry.[9]

Since the discovery of the wild type GFP, numerous different mutants of GFP have been engineered and tested.[23] The first significant leap forward was a single point mutation (S65T) reported by Tsien in 1995 in Nature.[24] This mutation dramatically improved the fluorescent (both intensity and photostability) and spectral characteristics of GFP. A shift of the major excitation peak to 488 nm with the emission peak staying at 509 nm thus can be clearly observed, which matched very well the spectral characteristics of commonly available FITC facilities. All these then largely amplified the practicality of using GFP by scientists in their research. Tsien mainly contributed to much of our understanding of how GFP works and for developing new techniques and mutants of GFP.

Former trainees of Roger Y. Tsien include Atsushi Miyawaki and Alice Y. Ting.

Timelines of GFP-development involved by Tsien:[22]

  • 1994: Tsien showed the mechanism that GFP chromophore is formed in a chemical reaction which requires oxygen but without help from the other proteins.
  • 1994–1998: Tsien and collaborators made various GFP mutants by genetic modification and structural tweaking. Newly created variants of GFP can shine more brightly and show different colours, such as yellow, cyan, and blue.
  • 2000–2002: Tsien produced monomeric variants of DsRED, which can glow in shades of red, pink, and orange. Remarkably, since then complicated marcromolecular networks of living organisms can be labelled or marked by using "all the colours of the rainbow".

Other detailed highlights involved by Tsien:[25]

  • 2002: The critical structural difference between GFP and DsRed was revealed. One extra double-bond in the chromophore of DsRed extends its conjugation thus causes the red-shift.
  • 2002: Monomeric DsRed (mRFP) was first developed.
  • 2004: New "fruit" FPs were generated (by in vitro and in vivo directed evolutions).

In 2009, a new kind of Infrared Fluorescent Protein (IFP) was developed by Tsien's group, and further reported and described by Science. The new IFPs are developed from bacterial phytochromes instead of from multicellular organism like jellyfish. Under normal conditions, bacterial phytochromes absorb light for signaling instead of fluorescence, but they can be turned fluorescent after deleting some of the signaling parts by genetic means such as site-directed mutagenesis. In order to fluoresce, IFPs require an exogenous chromophore, biliverdin.[26]

In 2016, a new class of fluorescent protein was evolved from a cyanobacterial (Trichodesmium erythraeum) phycobiliprotein, α-allophycocyanin, and named small ultra red fluorescent protein (smURFP). smURFP autocatalytically self-incorporates the chromophore biliverdin without the need of an external protein, known as a lyase.[27] Jellyfish- and coral-derived fluorescent proteins require oxygen and produce a stoichiometric amount of hydrogen peroxide upon chromophore formation.[28] smURFP does not require oxygen or produce hydrogen peroxide and uses the chromophore, biliverdin. smURFP has a large extinction coefficient (180,000 M−1 cm−1) and has a modest quantum yield (0.20), which makes it comparable biophysical brightness to eGFP and ~2-fold brighter than most red or far-red fluorescent proteins derived from coral. smURFP spectral properties are similar to the organic dye Cy5.[27]

Next generation sequencing Edit

Roger Tsien had built the foundation of next generation sequencing technology that is widely used today. On 26 October 1990, Roger Tsien et al. filed a patent of stepwise ("base-by-base") sequencing with removable 3' blockers on DNA arrays. Illumina integrated this concept with DNA cloning for their next generation sequencer.[29]

Calcium imaging Edit

Tsien was a pioneer of calcium imaging and known for developing various dyes which become fluorescent in the presence of particular ions such as calcium.[9][11] One such dye, fura-2, is widely used to track changes of calcium concentration within cells. indo-1 and fluo-3, other popular calcium indicators, were also developed by Tsien's group in 1985 and 1989 respectively. He has also developed fluorescent indicators for other ions such as magnesium, zinc, copper, iron, lead, cadmium, aluminum, nickel, cobalt, and mercury.[30]

Aequorin is also a useful tool to indicate calcium level inside cells; however, it has some limitations, primarily is that its prosthetic group coelenterazine is consumed irreversibly when emits light, thus requires continuous addition of coelenterazine into the media. To overcome such issues, Tsien's group also developed the calmodulin-based sensor, named Cameleon.[31]

FlAsH-EDT2 Edit

FlAsH-EDT2 is a biochemical method for specific covalent labeling of proteins harboring a tetracysteine motif (CCXXCC). It's a method based on recombinant protein molecules, and was developed by Tsien and his colleagues in 1998.[32]

  • "FLASH-EDT2": Fluorescein arsenical helix binder, bis-EDT adduct,
  • "EDT": 1,2-ethanedithiol.

Fluorescence-assisted cancer surgery Edit

Mouse experiments by Tsien's group suggest that cancer surgery can be guided and assisted by fluorescent peptides. The peptides are used as probes, and are harmless to living tissues and organs. Their lifetime in the body is only 4 or 5 days. Clinical trials are awaited.[33]

Industrial activities Edit

Tsien was also a notable biochemical inventor and holds or co-holds about 100 patents till 2010. In 1996, Tsien co-founded the Aurora Biosciences Corporation, which went public in 1997. In 2001, Aurora was acquired by the Vertex Pharmaceuticals. Similarly, Tsien was also a scientific co-founder of Senomyx in 1999.[12][34]

Tsien also promoted science education to promising young scientists through the first-ever San Diego Science Festival Lunch with a Laureate Program.[35]

Personal life Edit

According to the Qian (Tsien) clan genealogy book, Tsien is a 34th-generational descendant of King Qian Liu of the Wuyue Kingdom of ancient China. His family belongs to the line of King Qian Hongzong.[36] Tsien's parents Hsue-Chu Tsien and Yi-Ying Li (李懿颖) came from Hangzhou and Beijing, respectively.

Tsien had a number of engineers in his extended family, including his father Hsue-Chu Tsien who was an MIT-educated mechanical engineer and his mother's brothers Y. T. Li (李耀滋) and Shihying Lee (李诗颖), who were engineering professors at MIT. Tsien's mother Yi-Ying Li was a nurse.[15] The famous rocket scientist Tsien Hsue-shen, regarded as the co-founding father of the Jet Propulsion Laboratory of the California Institute of Technology and, later, the director of the Chinese ballistic-missile and space programs, is a cousin of Tsien's father.[37]

Tsien was the younger brother of Richard Tsien, a renowned neurobiologist currently at New York University,[38] and Louis Tsien, a software engineer. Tsien, who called his own work molecular engineering, once said, "I'm doomed by heredity to do this kind of work."[39]

He was married to Wendy Globe.[5]

Death Edit

Tsien died on August 24, 2016.[40] Although the specific cause of death was not disclosed, it was reported that he died while on a bike trail in Eugene, Oregon.[41] Prior to his death, Tsien had survived cancer and suffered a stroke in 2013.[42][43]

"He was ahead of us all," said Tsien's wife, Wendy. "He was ever the adventurer, the pathfinder, the free and soaring spirit. Courage, determination, creativity and resourcefulness were hallmarks of his character. He accomplished much. He will not be forgotten."[15]

Awards and honors Edit

Roger Y. Tsien has received numerous honors and awards in his life, including:

Named lectures and lectureships Edit

See also Edit

References Edit

  1. ^ a b Anon (2006). . London: Royal Society. Archived from the original on November 17, 2015. One or more of the preceding sentences incorporates text from the royalsociety.org website where:

    All text published under the heading 'Biography' on Fellow profile pages is available under Creative Commons Attribution 4.0 International License." --. Archived from the original on September 25, 2015. Retrieved March 9, 2016.{{cite web}}: CS1 maint: bot: original URL status unknown (link)
  2. ^ The Wolf Prize in Medicine in 2004 (detail)
  3. ^ a b Anon (2016). "Roger Tsien EMBO Profile". people.embo.org. Heidelberg: European Molecular Biology Organization.
  4. ^ a b Tsien, Roger Yongchien (1976). The design and use of organic chemical tools in cellular physiology (PhD thesis). University of Cambridge. OCLC 500581238.
  5. ^ a b c "Roger Y. Tsien, chemist shared Nobel for tool to research Alzheimer's, dies at 64". The Washington Post. August 31, 2016. Retrieved September 1, 2016.
  6. ^ Tsien, Roger Y. (1998). "The Green Fluorescent Protein". Annual Review of Biochemistry. 67 (1): 509–544. doi:10.1146/annurev.biochem.67.1.509. PMID 9759496.
  7. ^ a b . UCSD. 2008. Archived from the original (Official web page) on October 15, 2008. Retrieved November 4, 2008.
  8. ^ a b c "2008 Nobel Prize in Chemistry Laureates" (Official web page). The Nobel Foundation. October 8, 2008. Retrieved October 8, 2008.
  9. ^ a b c d Roger Y. Tsien on Nobelprize.org   with the Nobel lecture Constructing and Exploiting the Fluorescent Protein Paintbox
  10. ^ Tsien, Roger Y. (2010). "The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008". Journal of Visualized Experiments (35). doi:10.3791/1575. ISSN 1940-087X. PMC 3152217. PMID 20072108.  
  11. ^ a b Rudolf, Rüdiger; Mongillo, Marco; Rizzuto, Rosario; Pozzan, Tullio (2003). "Looking forward to seeing calcium" (PDF). Nature Reviews Molecular Cell Biology. 4 (7): 579–586. doi:10.1038/nrm1153. PMID 12838340. S2CID 28988011. Retrieved September 1, 2016.
  12. ^ a b c d Nicole Kresge, Robert D. Simoni, and Robert L. Hill. "The Chemistry of Fluorescent Indicators: the Work of Roger Y. Tsien", Journal of Biological Chemistry, September 15, 2006. Accessed September 18, 2007. "At age 16, Tsien won first prize in the nationwide Westinghouse talent search with a project investigating how metals bind to thiocyanate."
  13. ^ Swayze, Bill. "Jersey teens call science a winner: Two finalists say just being in Westinghouse talent competition is prize enough", The Star-Ledger, March 11, 1997. Accessed September 18, 2007. "Only one New Jersey teenager has ever captured top honors in the history of the competition. That was Roger Tsien in 1968. The then-16-year-old Livingston High School math-science whiz explored the way subatomic particles act as bridges between two dissimilar metal atoms in various complex molecules."
  14. ^ "Phi Beta Kappa" (Web page). The Harvard Crimson. April 24, 1971. Retrieved October 9, 2008.
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  16. ^ June Q. Wu; CRIMSON STAFF WRITER (October 9, 2008). "Harvard Alumni Win Nobel Prize – Three chemists share award for green fluorescent jellyfish protein" (Web page). The Harvard Crimson. Retrieved October 9, 2008.
  17. ^ a b c "Cambridge graduate wins Nobel Prize for Chemistry" (Web page). The University of Cambridge. October 8, 2008. Retrieved October 8, 2008.
  18. ^ Rink, Timothy J.; Tsien, Louis Y.; Tsien, Richard W. (October 13, 2016). "Roger Yonchien Tsien (1952–2016)". Nature. 538 (172): 172. Bibcode:2016Natur.538..172R. doi:10.1038/538172a. ISSN 0028-0836. PMC 5960232. PMID 27734865.
  19. ^ "HHMI Scientist Abstract: Roger Y. Tsien, PhD" (Web page). HHMI. August 20, 2007. Retrieved October 8, 2008.
  20. ^ "HHMI Scientist Bio: Roger Y. Tsien, PhD" (Web page). HHMI. Retrieved October 8, 2008.
  21. ^ "The Wolf Foundation Prize in Medicine" (Web page). The Wolf Foundation. 2004. Retrieved October 8, 2008.
  22. ^ a b (PDF). The Royal Swedish Academy of Sciences, Information Department. October 8, 2008. Archived from the original (PDF) on October 28, 2008. Retrieved October 8, 2008.
  23. ^ Shaner N, Steinbach P, Tsien R (2005). "A guide to choosing fluorescent proteins" (PDF). Nat Methods. 2 (12): 905–9. doi:10.1038/nmeth819. PMID 16299475. S2CID 10024284.
  24. ^ Heim R, Cubitt A, Tsien R (1995). "Improved green fluorescence" (PDF). Nature. 373 (6516): 663–4. Bibcode:1995Natur.373..663H. doi:10.1038/373663b0. PMID 7854443. S2CID 40179694.
  25. ^ "Green Fluorescent Protein: Timeline" (Web page). Conncoll.edu. November 18, 2009. Retrieved December 22, 2009.
  26. ^ Xiaokun Shu; Antoine Royant; Michael Z. Lin; Todd A. Aguilera; Varda Lev-Ram; Paul A. Steinbach; Roger Y. Tsien (2009). "Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome". Science. 324 (5928): 804–07. Bibcode:2009Sci...324..804S. doi:10.1126/science.1168683. PMC 2763207. PMID 19423828.
  27. ^ a b Rodriguez, Erik A.; Tran, Geraldine N.; Gross, Larry A.; Crisp, Jessica L.; Shu, Xiaokun; Lin, John Y.; Tsien, Roger Y. (August 1, 2016). "A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein". Nature Methods. 13 (9): 763–9. doi:10.1038/nmeth.3935. ISSN 1548-7105. PMC 5007177. PMID 27479328.
  28. ^ Tsien, Roger Y. (January 1, 1998). "The Green Fluorescent Protein". Annual Review of Biochemistry. 67 (1): 509–544. doi:10.1146/annurev.biochem.67.1.509. PMID 9759496.
  29. ^ "Espacenet – Bibliographic data". worldwide.espacenet.com.
  30. ^ "Fluorescent Indicators for Zn2+ and Other Metal Ions—Section 19.7 – US".
  31. ^ Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikurak M, Tsien RY (1997). "Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882–7. Bibcode:1997Natur.388..882M. doi:10.1038/42264. PMID 9278050. S2CID 13745050.
  32. ^ B. Albert Griffin; Stephen R. Adams; Roger Y. Tsien (1998). "Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells". Science. 281 (5374): 269–72. Bibcode:1998Sci...281..269G. doi:10.1126/science.281.5374.269. PMID 9657724.
  33. ^ "PNAS: Fluorescence + MR imaging probe can guide cancer surgery" (php). PNAS. March 4, 2010. Retrieved March 11, 2010.
  34. ^ Interview, Roger Tsien. The light fantastic, pages 7–9 in The Biologist, Biochemistry Supplement. Vol 62 No 5 supplement. Oct/Nov 2015
  35. ^ San Diego Science Festival Lunch with a Laureate
  36. ^ 诺贝尔化学奖得主钱永健系吴越国王34世孙 [Nobel Chemistry Prize winner Roger Tsien is the 34th-generational descendant of the King of Wuyue] (in Chinese). Sina.com. October 9, 2008. Retrieved October 9, 2008.
  37. ^ Ruth Williams; Horsfall, MJ; Van Helten, JB; Glickman, BW; Mohn, GR (October 8, 2007). "People & Ideas – Roger Tsien: Bringing color to cell biology". J Cell Biol. 179 (1): 6–8. doi:10.1083/jcb.1791pi. PMC 2064723. PMID 17923526.
  38. ^ Ma, Huan; Li, Boxing; Tsien, Richard W. (2015). "Distinct roles of multiple isoforms of CaMKII in signaling to the nucleus". Biochimica et Biophysica Acta (BBA) – Molecular Cell Research. 1853 (9): 1953–1957. doi:10.1016/j.bbamcr.2015.02.008. ISSN 0167-4889. PMC 4522395. PMID 25700840.
  39. ^ Steele, D. (2004) Cells aglow. HHMI Bulletin, Summer 2004, 22–26
  40. ^ Huang, Christopher L.-H. (2018). "Roger Yonchien Tsien. 1 February 1952—24 August 2016". Biographical Memoirs of Fellows of the Royal Society. doi:10.1098/rsbm.2018.0013
  41. ^ Lippard, Stephen J. (October 7, 2016). "Roger Y. Tsien (1952–2016)". Science. 354 (6038): 41. Bibcode:2016Sci...354...41L. doi:10.1126/science.aak9585. PMID 27846487. S2CID 3208420.
  42. ^ Potter, Matt (September 21, 2016). "Details scarce in death of Nobel Prize–winner". San Diego Reader. Retrieved May 6, 2017.
  43. ^ Hagerty, James R. (September 9, 2016). "Nobel-Winning Chemist Created a Rainbow of Colors to Illuminate Cells". Wall Street Journal. Retrieved May 6, 2017.
  44. ^ Barbara Bachtler (November 21, 2002). "Prof. Roger Tsien Honoured with Max Delbrueck Medal" (Web page). Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch. Retrieved October 8, 2008.
  45. ^ . Rosenstiel Basic Medical Sciences Research Center. 2006. Archived from the original on June 23, 2010. Retrieved December 31, 2009.
  46. ^ "New Foreign members & Honorary Fellow 2006" (Web page). The Royal Society. 2006. Retrieved September 27, 2008.
  47. ^ "Who are our Honorary Fellows?" (asp). The Royal Society of Chemistry. Retrieved December 27, 2009.
  48. ^ "New Nobel laureate to be named honorary academician". The China Post. Taiwan (ROC). October 16, 2008. Retrieved December 27, 2009.
  49. ^ Roger S. Dong (April 2009). "2009 AAEOY Awards" (PDF). National Engineers Week Foundation. Retrieved December 27, 2009.
  50. ^ "Salute to Innovation" (shtml). UC San Diego Technology Transfer Office (TTO). May 20, 2009. Retrieved May 20, 2009.
  51. ^ "HKU to award Honorary Degree to Nobel Laureate Professor Roger Yonchien Tsien". The University of Hong Kong. October 29, 2009. Retrieved October 29, 2009.
  52. ^ "The Chinese University of Hong Kong Holds 67th Congregation for Conferment of Degrees". The Chinese University of Hong Kong. December 10, 2009. Retrieved December 10, 2009.
  53. ^ "PM Dr. Manmohan Singh gives away awards to prominent scientists" (asp). India Education Dairy.com. January 3, 2010. Retrieved January 4, 2010.
  54. ^ "RSC Spiers Memorial Award 2010 winner – Roger Tsien, UCSD, USA" (asp). The Royal Society of Chemistry. 2009. Retrieved December 22, 2009.
  55. ^ "Green Fluorescent Protein". The Golden Goose Award. Retrieved May 27, 2015.
  56. ^ "Golden Plate Awardees of the American Academy of Achievement". www.achievement.org. American Academy of Achievement.
  57. ^ "Bowditch Award Lecture". Recipients. American Physiological Society. Retrieved March 23, 2015.
  58. ^ 本院邀請諾貝爾化學獎得主錢永健教授12月蒞臨「中央研究院講座」演講 (in Chinese). Academia Sinica. December 11, 2009. Retrieved December 27, 2009.
  59. ^ "Academia Sinica to welcome lectures by 2008 Nobel laureate". eTaiwan News (Central News Agency). December 11, 2009. Retrieved December 27, 2009.
  60. ^ "The 2010 National Lecture will be given by Roger Tsien, UCSD" (aspx). The Biophysical Society. 2009. Retrieved September 10, 2009.
  61. ^ "UCL 2011 Prize Lecture in Clinical Science with Roger Tsien | UCL Events".

External links Edit

  •   Media related to Roger Tsien at Wikimedia Commons
  • Roger Y. Tsien on Nobelprize.org  

October 21, 2023
roger, tsien, roger, yonchien, tsien, pronounced, chen, february, 1952, august, 2016, american, biochemist, professor, chemistry, biochemistry, university, california, diego, awarded, nobel, prize, chemistry, 2008, discovery, development, green, fluorescent, p. Roger Yonchien Tsien pronounced tʃ ɛ n CHEN February 1 1952 August 24 2016 was an American biochemist He was a professor of chemistry and biochemistry at the University of California San Diego 7 and was awarded the Nobel Prize in Chemistry in 2008 for his discovery and development of the green fluorescent protein in collaboration with organic chemist Osamu Shimomura and neurobiologist Martin Chalfie 8 9 10 Tsien was also a pioneer of calcium imaging 9 11 Roger TsienTsien in 2008BornRoger Yonchien Tsien 1952 02 01 February 1 1952New York City U S DiedAugust 24 2016 2016 08 24 aged 64 Eugene Oregon U S 5 NationalityAmericanCitizenshipUnited StatesEducationHarvard University AB University of Cambridge PhD Known forGreen fluorescent protein 6 Calcium imagingSpouseWendy Globe 5 AwardsNobel Prize in Chemistry 2008 Physiological Society Annual Review Prize Lecture 2014 Golden Goose Award 2012 E B Wilson Medal 2008 Rosenstiel Award 2006 ForMemRS 2006 1 Wolf Prize in Medicine 2004 2 Keio Medical Science Prize 2004 EMBO Membership 2005 3 Dr A H Heineken Prize 2002 Artois Baillet Latour Health Prize 1995 Gairdner Foundation International Award 1995 Scientific careerFieldsBiochemistryInstitutionsUniversity of California San Diego University of California BerkeleyThesisThe design and use of organic chemical tools in cellular physiology 1976 Doctoral advisorRichard Adrian 4 Chinese nameTraditional Chinese錢永健Simplified Chinese钱永健TranscriptionsStandard MandarinHanyu PinyinQian YǒngjianWade GilesCh ien Yung chienWebsitewww wbr tsienlab wbr ucsd wbr edu Contents 1 Early life 2 Education 3 Research and career 3 1 Fluorescent proteins 3 2 Next generation sequencing 3 3 Calcium imaging 3 4 FlAsH EDT2 3 5 Fluorescence assisted cancer surgery 3 6 Industrial activities 4 Personal life 4 1 Death 5 Awards and honors 5 1 Named lectures and lectureships 6 See also 7 References 8 External linksEarly life Edit nbsp Paul Krugman Roger Tsien Martin Chalfie Osamu Shimomura Makoto Kobayashi and Toshihide Masukawa Nobel Prize Laureates 2008 at a press conference at the Swedish Academy of Science in Stockholm Tsien was born to a Chinese American family in New York in 1952 12 He grew up in Livingston New Jersey 12 and attended Livingston High School 13 Tsien traces his family ancestry to Hangzhou China His father Hsue Chu Tsien an MIT and Shanghai Jiao Tong University alumnus was a mechanical engineer and had excelled academically graduating at the top of his university class Tsien suffered from asthma as a child and as a result he was often indoors He spent hours conducting chemistry experiments in his basement laboratory When he was 16 he won first prize in the nationwide Westinghouse Talent Search with a project investigating how metals bind to thiocyanate 12 Education EditTsien attended Harvard College on a National Merit Scholarship where he was elected to Phi Beta Kappa as a junior 14 He graduated summa cum laude with a Bachelor of Arts in chemistry and physics in 1972 15 According to his freshman year roommate economist and Iowa politician Herman Quirmbach It s probably not an exaggeration to say he s the smartest person I ever met a nd I have met a lot of brilliant people 16 After completing his bachelor s degree Tsien joined the Physiological Laboratory at the University of Cambridge in Cambridge England with the aid of a Marshall Scholarship and resided at Churchill College Cambridge 17 He received his Ph D in physiology in 1977 for research on The Design and Use of Organic Chemical Tools in Cellular Physiology 4 formally supervised by Richard Adrian in the department of physiology and assisted by Andy Holmes Gerry Smith and Jeremy Sanders in the department of chemistry 17 Research and career EditFollowing his Ph D Tsien was a research fellow at Gonville and Caius College Cambridge from 1977 to 1981 17 He was appointed to the faculty at the University of California Berkeley from 1982 to 1989 18 Beginning in 1989 he worked at the University of California San Diego as professor of pharmacology and professor of chemistry and biochemistry 7 and as an investigator of the Howard Hughes Medical Institute 19 20 Tsien contributed to the fields of cell biology and neurobiology by discovering genetically programmable fluorescent tags thereby allowing scientists to watch the behavior of molecules in living cells in real time He also developed fluorescent indicators of calcium ions and other ions important in biological processes nbsp GFP Movie showing entire structure and zoom in to fluorescent chromophore Movie created by Erik A Rodriguez with UCSF Chimera from PDB 1EMA in memory of Roger Y Tsien for the Birch Aquarium In 2004 Tsien was awarded the Wolf Prize in Medicine for his seminal contribution to the design and biological application of novel fluorescent and photolabile molecules to analyze and perturb cell signal transduction 21 In 2008 Tsien shared the Nobel Prize in Chemistry with Osamu Shimomura and Martin Chalfie for the green fluorescent protein discovery expression and development 8 22 Fluorescent proteins Edit The multicolored fluorescent proteins developed in Tsien s lab are used by scientists to track where and when certain genes are expressed in cells or in whole organisms Typically the gene coding for a protein of interest is fused with the gene for a fluorescent protein which causes the protein of interest to glow inside the cell when the cell is irradiated with a suitable wavelength of light and allows microscopists to track its location in real time This is such a popular technique that it has added a new dimension to the fields of molecular biology cell biology and biochemistry 9 Since the discovery of the wild type GFP numerous different mutants of GFP have been engineered and tested 23 The first significant leap forward was a single point mutation S65T reported by Tsien in 1995 in Nature 24 This mutation dramatically improved the fluorescent both intensity and photostability and spectral characteristics of GFP A shift of the major excitation peak to 488 nm with the emission peak staying at 509 nm thus can be clearly observed which matched very well the spectral characteristics of commonly available FITC facilities All these then largely amplified the practicality of using GFP by scientists in their research Tsien mainly contributed to much of our understanding of how GFP works and for developing new techniques and mutants of GFP Former trainees of Roger Y Tsien include Atsushi Miyawaki and Alice Y Ting Timelines of GFP development involved by Tsien 22 1994 Tsien showed the mechanism that GFP chromophore is formed in a chemical reaction which requires oxygen but without help from the other proteins 1994 1998 Tsien and collaborators made various GFP mutants by genetic modification and structural tweaking Newly created variants of GFP can shine more brightly and show different colours such as yellow cyan and blue 2000 2002 Tsien produced monomeric variants of DsRED which can glow in shades of red pink and orange Remarkably since then complicated marcromolecular networks of living organisms can be labelled or marked by using all the colours of the rainbow Other detailed highlights involved by Tsien 25 2002 The critical structural difference between GFP and DsRed was revealed One extra double bond in the chromophore of DsRed extends its conjugation thus causes the red shift 2002 Monomeric DsRed mRFP was first developed 2004 New fruit FPs were generated by in vitro and in vivo directed evolutions In 2009 a new kind of Infrared Fluorescent Protein IFP was developed by Tsien s group and further reported and described by Science The new IFPs are developed from bacterial phytochromes instead of from multicellular organism like jellyfish Under normal conditions bacterial phytochromes absorb light for signaling instead of fluorescence but they can be turned fluorescent after deleting some of the signaling parts by genetic means such as site directed mutagenesis In order to fluoresce IFPs require an exogenous chromophore biliverdin 26 In 2016 a new class of fluorescent protein was evolved from a cyanobacterial Trichodesmium erythraeum phycobiliprotein a allophycocyanin and named small ultra red fluorescent protein smURFP smURFP autocatalytically self incorporates the chromophore biliverdin without the need of an external protein known as a lyase 27 Jellyfish and coral derived fluorescent proteins require oxygen and produce a stoichiometric amount of hydrogen peroxide upon chromophore formation 28 smURFP does not require oxygen or produce hydrogen peroxide and uses the chromophore biliverdin smURFP has a large extinction coefficient 180 000 M 1 cm 1 and has a modest quantum yield 0 20 which makes it comparable biophysical brightness to eGFP and 2 fold brighter than most red or far red fluorescent proteins derived from coral smURFP spectral properties are similar to the organic dye Cy5 27 Next generation sequencing Edit Roger Tsien had built the foundation of next generation sequencing technology that is widely used today On 26 October 1990 Roger Tsien et al filed a patent of stepwise base by base sequencing with removable 3 blockers on DNA arrays Illumina integrated this concept with DNA cloning for their next generation sequencer 29 Calcium imaging Edit Tsien was a pioneer of calcium imaging and known for developing various dyes which become fluorescent in the presence of particular ions such as calcium 9 11 One such dye fura 2 is widely used to track changes of calcium concentration within cells indo 1 and fluo 3 other popular calcium indicators were also developed by Tsien s group in 1985 and 1989 respectively He has also developed fluorescent indicators for other ions such as magnesium zinc copper iron lead cadmium aluminum nickel cobalt and mercury 30 Aequorin is also a useful tool to indicate calcium level inside cells however it has some limitations primarily is that its prosthetic group coelenterazine is consumed irreversibly when emits light thus requires continuous addition of coelenterazine into the media To overcome such issues Tsien s group also developed the calmodulin based sensor named Cameleon 31 FlAsH EDT2 Edit FlAsH EDT2 is a biochemical method for specific covalent labeling of proteins harboring a tetracysteine motif CCXXCC It s a method based on recombinant protein molecules and was developed by Tsien and his colleagues in 1998 32 FLASH EDT2 Fluorescein arsenical helix binder bis EDT adduct EDT 1 2 ethanedithiol Fluorescence assisted cancer surgery Edit Mouse experiments by Tsien s group suggest that cancer surgery can be guided and assisted by fluorescent peptides The peptides are used as probes and are harmless to living tissues and organs Their lifetime in the body is only 4 or 5 days Clinical trials are awaited 33 Industrial activities Edit Tsien was also a notable biochemical inventor and holds or co holds about 100 patents till 2010 In 1996 Tsien co founded the Aurora Biosciences Corporation which went public in 1997 In 2001 Aurora was acquired by the Vertex Pharmaceuticals Similarly Tsien was also a scientific co founder of Senomyx in 1999 12 34 Tsien also promoted science education to promising young scientists through the first ever San Diego Science Festival Lunch with a Laureate Program 35 Personal life EditAccording to the Qian Tsien clan genealogy book Tsien is a 34th generational descendant of King Qian Liu of the Wuyue Kingdom of ancient China His family belongs to the line of King Qian Hongzong 36 Tsien s parents Hsue Chu Tsien and Yi Ying Li 李懿颖 came from Hangzhou and Beijing respectively Tsien had a number of engineers in his extended family including his father Hsue Chu Tsien who was an MIT educated mechanical engineer and his mother s brothers Y T Li 李耀滋 and Shihying Lee 李诗颖 who were engineering professors at MIT Tsien s mother Yi Ying Li was a nurse 15 The famous rocket scientist Tsien Hsue shen regarded as the co founding father of the Jet Propulsion Laboratory of the California Institute of Technology and later the director of the Chinese ballistic missile and space programs is a cousin of Tsien s father 37 Tsien was the younger brother of Richard Tsien a renowned neurobiologist currently at New York University 38 and Louis Tsien a software engineer Tsien who called his own work molecular engineering once said I m doomed by heredity to do this kind of work 39 He was married to Wendy Globe 5 Death Edit Tsien died on August 24 2016 40 Although the specific cause of death was not disclosed it was reported that he died while on a bike trail in Eugene Oregon 41 Prior to his death Tsien had survived cancer and suffered a stroke in 2013 42 43 He was ahead of us all said Tsien s wife Wendy He was ever the adventurer the pathfinder the free and soaring spirit Courage determination creativity and resourcefulness were hallmarks of his character He accomplished much He will not be forgotten 15 Awards and honors EditRoger Y Tsien has received numerous honors and awards in his life including National 1st Prize Westinghouse Science Talent Search 1968 National Merit Scholarship US 1968 Detur Prize Harvard College 1969 Marshall Scholarship British government 1972 Comyns Berkeley Research Fellowship Gonville and Caius College Cambridge 1977 Gedge Prize University of Cambridge 1978 Searle Scholar Searle Scholar program 1983 Lamport Prize New York Academy of Sciences 1986 Javits Neuroscience Investigator Award National Institute of Neurological Disorders and Stroke 1989 Young Scientist Award Passano Foundation 1991 W Alden Spencer Award in Neurobiology Columbia University 1991 Artois Baillet Latour Health Prize Belgium 1995 Gairdner Foundation International Award Canada 1995 Basic Research Prize American Heart Association 1995 Elected to the United States Institute of Medicine 1995 Doctorate honoris causa Katholieke Universiteit Leuven Belgium 1995 Faculty Research Lecturer UC San Diego 1997 Elected to the American Academy of Arts and Sciences 1998 Elected to the United States National Academy of Sciences 1998 Award for Innovation in High Throughput Screening Society for Biomolecular Screening 1998 Pearse Prize Royal Microscopical Society 2000 ACS Award for Creative Invention American Chemical Society 2002 Christian B Anfinsen Award Protein Society 2002 Heineken Prize for Biochemistry and Biophysics Royal Netherlands Academy of Arts and Sciences 2002 Max Delbruck Medal Max Delbruck Centrum fur Molekulare Medizin Berlin 2002 44 Wolf Prize in Medicine Israel 2004 Keio Medical Science Prize Japan 2004 UCSD Chancellor s Associates Award for Excellence in Science amp Engineering Research UC San Diego 2004 Perl UNC Neuroscience Prize University of North Carolina 2004 Associate EMBO Member of the European Molecular Biology Organization 2005 3 J Allyn Taylor International Prize in Medicine Robarts Research Institute Canada 2005 ABRF Award Association of Biomolecular Resource Facilities 2006 Lewis S Rosenstiel Award for Distinguished Work in the Basic Medical Sciences Brandeis University 2006 45 Elected a Foreign Member of the Royal Society ForMemRS in 2006 1 46 BioPharma Leadership Award the 6th Annual San Diego BioPharma Conference San Diego 2007 US Department of Defense DoD Breast Cancer Innovator Award Nobel Prize in Chemistry Sweden 2008 8 E B Wilson Medal American Society for Cell Biology 2008 Honorary Fellow of the Royal Society of Chemistry HonFRSC UK 2008 47 Honorary Academician Academia Sinica 2008 48 February 18 2009 Roger Tsien Day in the City of San Diego California US Distinguished Science and Technology Award The 2009 Asian American Engineers of the Year AAEoY Award April 2009 49 Lifetime Innovation Award UC San Diego May 20 2009 50 AHA Distinguished Scientists American Heart Association 2009 Molecular Imaging Achievement Award Society of Molecular Imaging 2009 Doctor of Science honoris causa The University of Hong Kong 2009 51 Doctor of Science honoris causa Chinese University of Hong Kong 2009 52 General President Gold Medal the 97th Indian Science Congress India January 3 2010 53 Spiers Memorial Award Royal Society of Chemistry UK 2010 54 Golden Goose Award 2012 55 Golden Plate Award of the American Academy of Achievement 2012 56 Named lectures and lectureships Edit Bowditch Lectureship American Physiological Society 1992 57 Hans L Falk Memorial Lectureship National Institute of Environmental Health Sciences 1993 Quastel Lectureship Hebrew University of Jerusalem 1994 President s Lectureship American Thoracic Society 1994 Roger Eckert Memorial Lecture Gottingen Neurobiology Conference of the German Neuroscience Society 1995 Melvin Calvin Lectureship UC Berkeley 1999 Herbert Sober Lectureship American Society for Biochemistry and Molecular Biology 2000 Keith Porter Lecture American Society for Cell Biology 2003 Konrad Bloch Lectureship Harvard University 2003 Grass Foundation Lectureship Society for Neuroscience 2004 The 1st Academia Sinica Lecturer the highest honor of the academy Dec 2009 58 59 The 2010 National Lecturer of the Biophysical Society the highest honor of the society 60 The 2011 UCL Prize Lecture in Clinical Science University College London 61 See also EditQuyen T NguyenReferences Edit a b Anon 2006 Professor Roger Tsien ForMemRS London Royal Society Archived from the original on November 17 2015 One or more of the preceding sentences incorporates text from the royalsociety org website where All text published under the heading Biography on Fellow profile pages is available under Creative Commons Attribution 4 0 International License Royal Society Terms conditions and policies Archived from the original on September 25 2015 Retrieved March 9 2016 a href Template Cite web html title Template Cite web cite web a CS1 maint bot original URL status unknown link The Wolf Prize in Medicine in 2004 detail a b Anon 2016 Roger Tsien EMBO Profile people embo org Heidelberg European Molecular Biology Organization a b Tsien Roger Yongchien 1976 The design and use of organic chemical tools in cellular physiology PhD thesis University of Cambridge OCLC 500581238 a b c Roger Y Tsien chemist shared Nobel for tool to research Alzheimer s dies at 64 The Washington Post August 31 2016 Retrieved September 1 2016 Tsien Roger Y 1998 The Green Fluorescent Protein Annual Review of Biochemistry 67 1 509 544 doi 10 1146 annurev biochem 67 1 509 PMID 9759496 a b Roger Tsien at UCSD Department of Chemistry amp Biochemistry UCSD 2008 Archived from the original Official web page on October 15 2008 Retrieved November 4 2008 a b c 2008 Nobel Prize in Chemistry Laureates Official web page The Nobel Foundation October 8 2008 Retrieved October 8 2008 a b c d Roger Y Tsien on Nobelprize org nbsp with the Nobel lecture Constructing and Exploiting the Fluorescent Protein Paintbox Tsien Roger Y 2010 The 2009 Lindau Nobel Laureate Meeting Roger Y Tsien Chemistry 2008 Journal of Visualized Experiments 35 doi 10 3791 1575 ISSN 1940 087X PMC 3152217 PMID 20072108 nbsp a b Rudolf Rudiger Mongillo Marco Rizzuto Rosario Pozzan Tullio 2003 Looking forward to seeing calcium PDF Nature Reviews Molecular Cell Biology 4 7 579 586 doi 10 1038 nrm1153 PMID 12838340 S2CID 28988011 Retrieved September 1 2016 a b c d Nicole Kresge Robert D Simoni and Robert L Hill The Chemistry of Fluorescent Indicators the Work of Roger Y Tsien Journal of Biological Chemistry September 15 2006 Accessed September 18 2007 At age 16 Tsien won first prize in the nationwide Westinghouse talent search with a project investigating how metals bind to thiocyanate Swayze Bill Jersey teens call science a winner Two finalists say just being in Westinghouse talent competition is prize enough The Star Ledger March 11 1997 Accessed September 18 2007 Only one New Jersey teenager has ever captured top honors in the history of the competition That was Roger Tsien in 1968 The then 16 year old Livingston High School math science whiz explored the way subatomic particles act as bridges between two dissimilar metal atoms in various complex molecules Phi Beta Kappa Web page The Harvard Crimson April 24 1971 Retrieved October 9 2008 a b c LaFee Scott August 31 2016 Nobel Laureate Roger Tsien Dies Age 64 UC San Diego News Center Retrieved August 31 2016 June Q Wu CRIMSON STAFF WRITER October 9 2008 Harvard Alumni Win Nobel Prize Three chemists share award for green fluorescent jellyfish protein Web page The Harvard Crimson Retrieved October 9 2008 a b c Cambridge graduate wins Nobel Prize for Chemistry Web page The University of Cambridge October 8 2008 Retrieved October 8 2008 Rink Timothy J Tsien Louis Y Tsien Richard W October 13 2016 Roger Yonchien Tsien 1952 2016 Nature 538 172 172 Bibcode 2016Natur 538 172R doi 10 1038 538172a ISSN 0028 0836 PMC 5960232 PMID 27734865 HHMI Scientist Abstract Roger Y Tsien PhD Web page HHMI August 20 2007 Retrieved October 8 2008 HHMI Scientist Bio Roger Y Tsien PhD Web page HHMI Retrieved October 8 2008 The Wolf Foundation Prize in Medicine Web page The Wolf Foundation 2004 Retrieved October 8 2008 a b The green fluorescent protein discovery expression and development PDF The Royal Swedish Academy of Sciences Information Department October 8 2008 Archived from the original PDF on October 28 2008 Retrieved October 8 2008 Shaner N Steinbach P Tsien R 2005 A guide to choosing fluorescent proteins PDF Nat Methods 2 12 905 9 doi 10 1038 nmeth819 PMID 16299475 S2CID 10024284 Heim R Cubitt A Tsien R 1995 Improved green fluorescence PDF Nature 373 6516 663 4 Bibcode 1995Natur 373 663H doi 10 1038 373663b0 PMID 7854443 S2CID 40179694 Green Fluorescent Protein Timeline Web page Conncoll edu November 18 2009 Retrieved December 22 2009 Xiaokun Shu Antoine Royant Michael Z Lin Todd A Aguilera Varda Lev Ram Paul A Steinbach Roger Y Tsien 2009 Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome Science 324 5928 804 07 Bibcode 2009Sci 324 804S doi 10 1126 science 1168683 PMC 2763207 PMID 19423828 a b Rodriguez Erik A Tran Geraldine N Gross Larry A Crisp Jessica L Shu Xiaokun Lin John Y Tsien Roger Y August 1 2016 A far red fluorescent protein evolved from a cyanobacterial phycobiliprotein Nature Methods 13 9 763 9 doi 10 1038 nmeth 3935 ISSN 1548 7105 PMC 5007177 PMID 27479328 Tsien Roger Y January 1 1998 The Green Fluorescent Protein Annual Review of Biochemistry 67 1 509 544 doi 10 1146 annurev biochem 67 1 509 PMID 9759496 Espacenet Bibliographic data worldwide espacenet com Fluorescent Indicators for Zn2 and Other Metal Ions Section 19 7 US Miyawaki A Llopis J Heim R McCaffery JM Adams JA Ikurak M Tsien RY 1997 Fluorescent indicators for Ca2 based on green fluorescent proteins and calmodulin Nature 388 6645 882 7 Bibcode 1997Natur 388 882M doi 10 1038 42264 PMID 9278050 S2CID 13745050 B Albert Griffin Stephen R Adams Roger Y Tsien 1998 Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells Science 281 5374 269 72 Bibcode 1998Sci 281 269G doi 10 1126 science 281 5374 269 PMID 9657724 PNAS Fluorescence MR imaging probe can guide cancer surgery php PNAS March 4 2010 Retrieved March 11 2010 Interview Roger Tsien The light fantastic pages 7 9 in The Biologist Biochemistry Supplement Vol 62 No 5 supplement Oct Nov 2015 San Diego Science Festival Lunch with a Laureate 诺贝尔化学奖得主钱永健系吴越国王34世孙 Nobel Chemistry Prize winner Roger Tsien is the 34th generational descendant of the King of Wuyue in Chinese Sina com October 9 2008 Retrieved October 9 2008 Ruth Williams Horsfall MJ Van Helten JB Glickman BW Mohn GR October 8 2007 People amp Ideas Roger Tsien Bringing color to cell biology J Cell Biol 179 1 6 8 doi 10 1083 jcb 1791pi PMC 2064723 PMID 17923526 Ma Huan Li Boxing Tsien Richard W 2015 Distinct roles of multiple isoforms of CaMKII in signaling to the nucleus Biochimica et Biophysica Acta BBA Molecular Cell Research 1853 9 1953 1957 doi 10 1016 j bbamcr 2015 02 008 ISSN 0167 4889 PMC 4522395 PMID 25700840 Steele D 2004 Cells aglow HHMI Bulletin Summer 2004 22 26 Huang Christopher L H 2018 Roger Yonchien Tsien 1 February 1952 24 August 2016 Biographical Memoirs of Fellows of the Royal Society doi 10 1098 rsbm 2018 0013 Lippard Stephen J October 7 2016 Roger Y Tsien 1952 2016 Science 354 6038 41 Bibcode 2016Sci 354 41L doi 10 1126 science aak9585 PMID 27846487 S2CID 3208420 Potter Matt September 21 2016 Details scarce in death of Nobel Prize winner San Diego Reader Retrieved May 6 2017 Hagerty James R September 9 2016 Nobel Winning Chemist Created a Rainbow of Colors to Illuminate Cells Wall Street Journal Retrieved May 6 2017 Barbara Bachtler November 21 2002 Prof Roger Tsien Honoured with Max Delbrueck Medal Web page Max Delbruck Center for Molecular Medicine MDC Berlin Buch Retrieved October 8 2008 Lewis S Rosenstiel Award for Distinguished Work in Basic Medical Science Past Winners Rosenstiel Basic Medical Sciences Research Center 2006 Archived from the original on June 23 2010 Retrieved December 31 2009 New Foreign members amp Honorary Fellow 2006 Web page The Royal Society 2006 Retrieved September 27 2008 Who are our Honorary Fellows asp The Royal Society of Chemistry Retrieved December 27 2009 New Nobel laureate to be named honorary academician The China Post Taiwan ROC October 16 2008 Retrieved December 27 2009 Roger S Dong April 2009 2009 AAEOY Awards PDF National Engineers Week Foundation Retrieved December 27 2009 Salute to Innovation shtml UC San Diego Technology Transfer Office TTO May 20 2009 Retrieved May 20 2009 HKU to award Honorary Degree to Nobel Laureate Professor Roger Yonchien Tsien The University of Hong Kong October 29 2009 Retrieved October 29 2009 The Chinese University of Hong Kong Holds 67th Congregation for Conferment of Degrees The Chinese University of Hong Kong December 10 2009 Retrieved December 10 2009 PM Dr Manmohan Singh gives away awards to prominent scientists asp India Education Dairy com January 3 2010 Retrieved January 4 2010 RSC Spiers Memorial Award 2010 winner Roger Tsien UCSD USA asp The Royal Society of Chemistry 2009 Retrieved December 22 2009 Green Fluorescent Protein The Golden Goose Award Retrieved May 27 2015 Golden Plate Awardees of the American Academy of Achievement www achievement org American Academy of Achievement Bowditch Award Lecture Recipients American Physiological Society Retrieved March 23 2015 本院邀請諾貝爾化學獎得主錢永健教授12月蒞臨 中央研究院講座 演講 in Chinese Academia Sinica December 11 2009 Retrieved December 27 2009 Academia Sinica to welcome lectures by 2008 Nobel laureate eTaiwan News Central News Agency December 11 2009 Retrieved December 27 2009 The 2010 National Lecture will be given by Roger Tsien UCSD aspx The Biophysical Society 2009 Retrieved September 10 2009 UCL 2011 Prize Lecture in Clinical Science with Roger Tsien UCL Events External links Edit nbsp Media related to Roger Tsien at Wikimedia Commons Roger Y Tsien on Nobelprize org nbsp Retrieved from https en wikipedia org w index php title Roger Y Tsien amp oldid 1172868782, wikipedia, wiki, book, books, library,

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