Double fertilization

Double fertilization is a complex fertilization mechanism of flowering plants (angiosperms). This process involves the joining of a female gametophyte (megagametophyte, also called the embryo sac) with two male gametes (sperm). It begins when a pollen grain adheres to the stigma of the carpel, the female reproductive structure of a flower. The pollen grain then takes in moisture and begins to germinate, forming a pollen tube that extends down toward the ovary through the style. The tip of the pollen tube then enters the ovary and penetrates through the micropyle opening in the ovule. The pollen tube proceeds to release the two sperm in the embryo sacs.

The parts of a flower

Double fertilization

Double fertilization in Arabidopsis

The cells of an embryo sac of an unfertilized ovule are 8 in number and arranged in the form of 3+2+3 (from top to bottom) i.e. 3 antipodal cells, 2 polar central cells, 2 synergids & 1 egg cell. One sperm fertilizes the egg cell and the other sperm combines with the two polar nuclei of the large central cell of the megagametophyte. The haploid sperm and haploid egg combine to form a diploid zygote, the process being called syngamy, while the other sperm and the two haploid polar nuclei of the large central cell of the megagametophyte form a triploid nucleus (triple fusion). Some plants may form polyploid nuclei. The large cell of the gametophyte will then develop into the endosperm, a nutrient-rich tissue which provides nourishment to the developing embryo. The ovary, surrounding the ovules, develops into the fruit, which protects the seeds and may function to disperse them.^[1]

The two central cell maternal nuclei (polar nuclei) that contribute to the endosperm, arise by mitosis from the same single meiotic product that gave rise to the egg. The maternal contribution to the genetic constitution of the triploid endosperm is double that of the sperm.

In a study conducted in 2008 of the plant Arabidopsis thaliana, the migration of male nuclei inside the female gamete, in fusion with the female nuclei, has been documented for the first time using in vivo imaging. Some of the genes involved in the migration and fusion process have also been determined.^[2]

Evidence of double fertilization in Gnetales, which are non-flowering seed plants, has been reported.^[3]

Brief history

Double fertilization was discovered more than a century ago by Sergei Nawaschin in Kyiv,^[4] and Léon Guignard in France. Each made the discovery independently of the other.^[5] Lilium martagon and Fritillaria tenella were used in the first observations of double fertilization, which were made using the classical light microscope. Due to the limitations of the light microscope, there were many unanswered questions regarding the process of double fertilization. However, with the development of the electron microscope, many of the questions were answered. Most notably, the observations made by the group of W. Jensen showed that the male gametes did not have any cell walls and that the plasma membrane of the gametes is close to the plasma membrane of the cell that surrounds them inside the pollen grain.^[6]

Double fertilization in gymnosperms

A far more rudimentary form of double fertilization occurs in the sexual reproduction of an order of gymnosperms commonly known as Gnetales.^[3] Specifically, this event has been documented in both Ephedra and Gnetum, a subset of Gnetophytes.^[7] In Ephedra nevadensis, a single binucleate sperm cell is deposited into the egg cell. Following the initial fertilization event, the second sperm nucleus is diverted to fertilize an additional egg nucleus found in the egg cytoplasm. In most other seed plants, this second 'ventral canal nucleus' is normally found to be functionally useless.^[8] In Gnetum gnemon, numerous free egg nuclei exist in female cytoplasm inside the female gametophyte. Succeeding the penetration of the mature female gametophyte by the pollen tube, female cytoplasm and free nuclei move to surround the pollen tube. Released from the binucleate sperm cell are two sperm nuclei which then join with free egg nuclei to produce two viable zygotes, a homologous characteristic between families Ephedra and Gnetum.^[9] In both families, the second fertilization event produces an additional diploid embryo. This supernumerary embryo is later aborted, leading to the synthesis of only one mature embryo.^[10] The additional fertilization product in Ephedra does not nourish the primary embryo, as the female gametophyte is responsible for nutrient provision.^[9] The more primitive process of double fertilization in gymnosperms results in two diploid nuclei enclosed in the same egg cell. This differs from the angiosperm condition, which results in the separation of the egg cell and endosperm.^[11] Comparative molecular research on the genome of G. gnemon has revealed that gnetophytes are more closely related to conifers than they are to angiosperms.^[12][13][14] The rejection of the anthophyte hypothesis, which identifies gnetales and angiosperms are sister taxa, leads to speculation that the process of double fertilization is a product of convergent evolution and arose independently among gnetophytes and angiosperms.^[15]

In vitro double fertilization

In vitro double fertilization is often used to study the molecular interactions as well as other aspects of gamete fusion in flowering plants. One of the major obstacles in developing an in vitro double fertilization between male and female gametes is the confinement of the sperm in the pollen tube and the egg in the embryo sac. A controlled fusion of the egg and sperm has already been achieved with poppy plants.^[16] Pollen germination, pollen tube entry, and double fertilization processes have all been observed to proceed normally. In fact, this technique has already been used to obtain seeds in various flowering plants and was named “test-tube fertilization”.^[17]

Related structures and functions

Megagametophyte

The female gametophyte, the megagametophyte, that participates in double fertilization in angiosperms which is haploid is called the embryo sac. This develops within an ovule, enclosed by the ovary at the base of a carpel. Surrounding the megagametophyte are (one or) two integuments, which form an opening called the micropyle. The megagametophyte, which is usually haploid, originates from the (usually diploid) megaspore mother cell, also called the megasporocyte. The next sequence of events varies, depending on the particular species, but in most species, the following events occur. The megasporocyte undergoes a meiotic cell division, producing four haploid megaspores. Only one of the four resulting megaspores survives. This megaspore undergoes three rounds of mitotic division, resulting in eight cells with eight haploid nuclei (the central cell has two nuclei, called the polar nuclei). The lower end of the embryo sac consists of the haploid egg cell positioned in the middle of two other haploid cells, called synergids. The synergids function in the attraction and guidance of the pollen tube to the megagametophyte through the micropyle. At the upper end of the megagametophyte are three antipodal cells.

Microgametophyte

The male gametophytes, or microgametophytes, that participate in double fertilization are contained within pollen grains. They develop within the microsporangia, or pollen sacs, of the anthers on the stamens. Each microsporangium contains diploid microspore mother cells, or microsporocytes. Each microsporocyte undergoes meiosis, forming four haploid microspores, each of which can eventually develop into a pollen grain. A microspore undergoes mitosis and cytokinesis in order to produce two separate cells, the generative cell and the tube cell. These two cells in addition to the spore wall make up an immature pollen grain. As the male gametophyte matures, the generative cell passes into the tube cell, and the generative cell undergoes mitosis, producing two sperm cells. Once the pollen grain has matured, the anthers break open, releasing the pollen. The pollen is carried to the pistil of another flower, by wind or animal pollinators, and deposited on the stigma. As the pollen grain germinates, the tube cell produces the pollen tube, which elongates and extends down the long style of the carpel and into the ovary, where its sperm cells are released in the megagametophyte. Double fertilization proceeds from here.^[18]

See also

• Fertilisation
• Gamete
• Megaspore
• Oosphere
• Ovule
• Pollen

References

1. Berger, F. (January 2008). "Double-fertilization, from myths to reality". Sexual Plant Reproduction. 21 (1): 3–5. doi:10.1007/s00497-007-0066-4. S2CID 8928640.
2. Berger, F.; Hamamura, Y. & Ingouff, M. & Higashiyama, T. (August 2008). "Double fertilization – Caught In The Act". Trends in Plant Science. 13 (8): 437–443. doi:10.1016/j.tplants.2008.05.011. PMID 18650119.{{cite journal}}: CS1 maint: multiple names: authors list (link)
3. V. Raghavan (September 2003). "Some reflections on double fertilization, from its discovery to the present". New Phytologist. 159 (3): 565–583. doi:10.1046/j.1469-8137.2003.00846.x. PMID 33873607.
4. Kordium EL (2008). "[Double fertilization in flowering plants: 1898-20&&&--". Tsitol. Genet. (in Russian). 42 (3): 12–26. PMID 18822860.
5. Jensen, W. A. (February 1998). "Double Fertilization: A Personal View". Sexual Plant Reproduction. 11 (1): 1–5. doi:10.1007/s004970050113. S2CID 33416360.
6. Dumas, C. & Rogowsky, P. (August 2008). "Fertilization and Early Seed Formation". Comptes Rendus Biologies. 331 (10): 715–725. doi:10.1016/j.crvi.2008.07.013. PMID 18926485.
7. Carmichael, J. S.; Friedman, W. E. (1995-12-01). "Double Fertilization in Gnetum gnemon: The Relationship between the Cell Cycle and Sexual Reproduction". The Plant Cell. 7 (12): 1975–1988. doi:10.1105/tpc.7.12.1975. ISSN 1040-4651. PMC 161055. PMID 12242365.
8. Friedman, William E. (1990). "Sexual Reproduction in Ephedra nevadensis (Ephedraceae): Further Evidence of Double Fertilization in a Nonflowering Seed Plant". American Journal of Botany. 77 (12): 1582–1598. doi:10.1002/j.1537-2197.1990.tb11399.x. JSTOR 2444491.
9. Carmichael, Jeffrey S.; Friedman, William E. (1996). "Double Fertilization in Gnetum gnemon (Gnetaceae): Its Bearing on the Evolution of Sexual Reproduction within the Gnetales and the Anthophyte Clade". American Journal of Botany. 83 (6): 767–780. doi:10.1002/j.1537-2197.1996.tb12766.x. JSTOR 2445854.
10. Friedman, W. E. (1995-04-25). "Organismal duplication, inclusive fitness theory, and altruism: understanding the evolution of endosperm and the angiosperm reproductive syndrome". Proceedings of the National Academy of Sciences. 92 (9): 3913–3917. Bibcode:1995PNAS...92.3913F. doi:10.1073/pnas.92.9.3913. ISSN 0027-8424. PMC 42072. PMID 11607532.
11. Friedman, William E. (1994). "The Evolution of Embryogeny in Seed Plants and the Developmental Origin and Early History of Endosperm". American Journal of Botany. 81 (11): 1468–1486. doi:10.1002/j.1537-2197.1994.tb15633.x. JSTOR 2445320.
12. Bowe, L. Michelle; Coat, Gwénaële; dePamphilis, Claude W. (2000-04-11). "Phylogeny of seed plants based on all three genomic compartments: Extant gymnosperms are monophyletic and Gnetales' closest relatives are conifers". Proceedings of the National Academy of Sciences. 97 (8): 4092–4097. Bibcode:2000PNAS...97.4092B. doi:10.1073/pnas.97.8.4092. ISSN 0027-8424. PMC 18159. PMID 10760278.
13. Winter, Kai-Uwe; Becker, Annette; Münster, Thomas; Kim, Jan T.; Saedler, Heinz; Theissen, Günter (1999-06-22). "MADS-box genes reveal that gnetophytes are more closely related to conifers than to flowering plants". Proceedings of the National Academy of Sciences. 96 (13): 7342–7347. Bibcode:1999PNAS...96.7342W. doi:10.1073/pnas.96.13.7342. ISSN 0027-8424. PMC 22087. PMID 10377416.
14. Magallon, S.; Sanderson, M. J. (2002-12-01). "Relationships among seed plants inferred from highly conserved genes: sorting conflicting phylogenetic signals among ancient lineages". American Journal of Botany. 89 (12): 1991–2006. doi:10.3732/ajb.89.12.1991. ISSN 1537-2197. PMID 21665628.
15. Chaw, Shu-Miaw; Parkinson, Christopher L.; Cheng, Yuchang; Vincent, Thomas M.; Palmer, Jeffrey D. (2000-04-11). "Seed plant phylogeny inferred from all three plant genomes: Monophyly of extant gymnosperms and origin of Gnetales from conifers". Proceedings of the National Academy of Sciences. 97 (8): 4086–4091. Bibcode:2000PNAS...97.4086C. doi:10.1073/pnas.97.8.4086. ISSN 0027-8424. PMC 18157. PMID 10760277.
16. Zenkteler, M. (1990). "In vitro fertilization and wide hybridization in higher plants". Crit Rev Plant Sci. 9 (3): 267–279. doi:10.1080/07352689009382290.
17. Raghavan, V. (2005). Double fertilization: embryo and endosperm development in flowering plants (illustrated ed.). Birkhäuser. pp. 17–19. ISBN 978-3-540-27791-0.
18. Campbell N.A.; Reece J.B. (2005). Biology (7 ed.). San Francisco, CA: Pearson Education, Inc. pp. 774–777. ISBN 978-0-8053-7171-0.