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C1orf27

January 01, 1970

Uncharacterized protein Chromosome 1 Open Reading Frame 27 is a protein in humans, encoded by the C1orf27 gene. It is accession number NM_017847.[1] This is a membrane protein that is 3926 base pairs long with the most extensive string of amino acids being 454aa long. C1orf27 exhibits cytoplasmic expression in epidermal tissues.[2] Predicted associated biological processes of the gene include cell fate specification and developmental properties.[citation needed]

Gene edit

Locus edit

This gene is located on chromosome 1 at 1q31.1.[3] It is encoded on the plus strand of DNA spanning from 186,344,406 to 186,390,514.

 
Diagram of C1orf27 and nearby genes on human chromosome 1.[3]

mRNA edit

Alternative splicing edit

There appear to be four isoforms due to splicing.[4] Two of those are truncated on the 3' end of the protein from 266aa and 396aa. Additional location of alternative splice sites are from 79aa to 102aa and 246aa to 260aa.

Protein edit

General properties edit

The primary encoded protein of C1orf27 consists of 454 amino acid residues and is 3926 base pairs long.[1] It consists of 14 total exons. The predicted molecular weight of the primary, unmodified protein is approximately 51.1 kdal.

Aliases edit

As with many other genes, there are some common aliases found with this gene.[citation needed] Those aliases are Lymphocyte-Activation Gene-1 (LAG1) Interacting Protein, Transparent Testa Glabra 1 (TTG1), and Odorant Response Abnormal 4 (ODR4). The most common alias for C1orf27 is ODR4, and this is what most readily appears when searching the gene.

Composition edit

Computational analysis revealed the most abundant amino acid to be leucine at 10.1% of the total protein.[5] The second most abundant was serine which contributes to 8.6% of the total protein. Glutamic acid was third most abundant and contributes to 7.7% of the protein. This analysis also revealed that the protein appears to be deficient in tryptophan as it only contributes to 1.1% of the protein.[5] Based on the distribution of other amino acid types, there were five high scoring hydrophobic segments. There were also two transmembrane domains located at 82-98aa and 432-449aa.

Post-translational modifications edit

Interactor Number of Predicted Sites Function
N-myristoylation8 Key components of signaling pathways, and typically promotes membrane binding essential for protein localization and/ or biological function[6]
N-glycosylation 4 Increase protein stability by decreasing protein dynamics.[7]
Protein Kinase C Phosphorylation7Enzymatic activity regulation.[8]
Casein Kinase II Phosphorylation 7 Epidermal growth factor role.[9]
Tyrosine Kinase Phosphorylation 2 Alterations to the structural conformation.[10]
cAMP and cGMP Dependent Phosphorylation 2 Coordination of the active site conformation and enzymatic activity.

C1orf27 is predicted to undergo multiple post translational modifications such as glycosylation, myristoylation, and phosphorylation.[11]

Interactions edit

There were eight interactions identified by Mentha.[12] The first one was UFSP2 which hydrolyzes the peptide bond at the C-term gly of UFM1, a ubiquitin-like modifier protein bound to a number of target proteins. The second one was HSCB which acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria. The third was GRB2 which is an adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. The fourth was CYLD which is a protease that cleaves Lys-63-linked polyubiquitin chains, controls regulation of cell survival, proliferation, and differentiation, and is required for normal cell cycle progress. The fifth was ATM which activates checkpoint signaling upon double strand breaks, apoptosis, and genotoxic stress. The sixth was FAM177A1, the function of which is unknown. The last two were THID2 and Q81kP6 which are both in bacillus anthracis.

Subcellular localization edit

The c1orf27 protein is likely cytoplasmic.[13] This was found with 55.5 reliability. The K-NN prediction was k=9/23 and the protein was found to be 55.6% cytoplasmic, 11.1% mitochondrial, 11.1% vacuolar, 11.1% cytoskeletal, and 11.1% golgi.

Structure edit

 
Predicted tertiary structure of c1orf27.[14]

Alpha helices predicted in the c1orf27 protein are colored blue in the above picture. Beta sheets are pictured by the red arrows. Random coils are the purple strands between structures.

Expression edit

 
Expression of C1orf27 in human tissues.[15]

Overall, expression of c1orf27 seems to be ubiquitous.[16] Highest expression body sites (>50 TPM) were bladder, bone marrow, kidney, liver, pancreas, parathyroid, and vascular. Highest expression health sites (>50 TPM) were adrenal tumors, cervical tumors, and liver tumors. While both of these observations had relatively high TPM scores, there was still relatively low occurrence. This validates the assumption that expression is ubiquitous. There was moderate expression (>25 TPM) in the human fetus, and expression increased with age.[16] Expression was completely absent in the ears, esophagus, lymph, nerve, salivary glands, thyroid, tonsils, and umbilical cord. There was no expression in bladder carcinoma despite expression being elevated in the bladder itself. There was high expression in endothelial cells and neuronal cells but was undetectable in glial cells and neuropil cells. Expression was also localized to the nucleoplasm and plasma membrane in humans but is localized to the cytosol in mice.

Homology edit

Paralogs edit

There were no paralogs of C1orf27 identified in the human genome.[4]

Orthologs edit

There were orthologs identified in most animals for which there were complete genome data.[4] The most distant, yet still relevant, orthologs identified were invertebrates from phylum Cnidaria.

Molecular Evolution edit

 
Rate of sequence divergence for C1orf27 in comparison to Fibrinogen and Cytochrome C in orthologs.

The m value, or number of corrected amino acid changes per 100 residues, for the C1orf27 gene was graphed against the species divergence in millions of years. When compared to divergence graphs of fibrinogen and cytochrome C, it was determined that this gene closely resembles the evolutionary pattern observed in fibrinogen, suggesting a more rapid rate of evolution. M values for C1orf27 were calculated using the percentage of identity, when compared to humans, observed in the mRNA sequences of the orthologs using the formula derived from the Molecular Clock Hypothesis.

References edit

  1. ^ a b "Homo sapiens odr-4 GPCR localization factor homolog (ODR4), transcript - Nucleotide - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-02-05.
  2. ^ "Tissue expression of C1orf27 - Summary - The Human Protein Atlas". www.proteinatlas.org. Retrieved 2018-02-05.
  3. ^ a b "ODR4 odr-4 GPCR localization factor homolog [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-02-19.
  4. ^ a b c "Protein BLAST: search protein databases using a protein query". blast.ncbi.nlm.nih.gov. Retrieved 2018-02-25.
  5. ^ a b EMBL-EBI. "SAPS < Sequence Statistics < EMBL-EBI". www.ebi.ac.uk. Retrieved 2018-04-22.
  6. ^ Wright MH, Heal WP, Mann DJ, Tate EW (March 2010). "Protein myristoylation in health and disease". Journal of Chemical Biology. 3 (1): 19–35. doi:10.1007/s12154-009-0032-8. PMC 2816741. PMID 19898886.
  7. ^ "Proteomics/Post-translational Modification/Glycosylation - Wikibooks, open books for an open world". en.wikibooks.org. Retrieved 2018-05-06.
  8. ^ Robles-Flores M, Meléndez L, García W, Mendoza-Hernández G, Lam TT, Castañeda-Patlán C, González-Aguilar H (May 2008). "Posttranslational modifications on protein kinase c isozymes. Effects of epinephrine and phorbol esters". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1783 (5): 695–712. doi:10.1016/j.bbamcr.2007.07.011. PMID 18295358.
  9. ^ Siomi MC, Higashijima K, Ishizuka A, Siomi H (December 2002). "Casein kinase II phosphorylates the fragile X mental retardation protein and modulates its biological properties". Molecular and Cellular Biology. 22 (24): 8438–47. doi:10.1128/MCB.22.24.8438-8447.2002. PMC 139871. PMID 12446764.
  10. ^ Rahimi N, Costello CE (January 2015). "Emerging roles of post-translational modifications in signal transduction and angiogenesis". Proteomics. 15 (2–3): 300–9. doi:10.1002/pmic.201400183. PMC 4297243. PMID 25161153.
  11. ^ "ExPASy - PROSITE". prosite.expasy.org. Retrieved 2018-04-22.
  12. ^ "mentha: the interactome browser". mentha.uniroma2.it. Retrieved 2018-04-22.
  13. ^ "PSORT II Prediction". psort.hgc.jp. Retrieved 2018-04-22.
  14. ^ Kelley, Lawrence. "PHYRE2 Protein Fold Recognition Server". www.sbg.bio.ic.ac.uk. Retrieved 2018-05-06.
  15. ^ "GDS1402 / NM_017847.1_PROBE1". www.ncbi.nlm.nih.gov. Retrieved 2018-05-06.
  16. ^ a b Group, Schuler. "EST Profile - Hs.371210". www.ncbi.nlm.nih.gov. Retrieved 2018-05-06.

January 01, 1970
c1orf27, uncharacterized, protein, chromosome, open, reading, frame, protein, humans, encoded, gene, accession, number, 017847, this, membrane, protein, that, 3926, base, pairs, long, with, most, extensive, string, amino, acids, being, 454aa, long, exhibits, c. Uncharacterized protein Chromosome 1 Open Reading Frame 27 is a protein in humans encoded by the C1orf27 gene It is accession number NM 017847 1 This is a membrane protein that is 3926 base pairs long with the most extensive string of amino acids being 454aa long C1orf27 exhibits cytoplasmic expression in epidermal tissues 2 Predicted associated biological processes of the gene include cell fate specification and developmental properties citation needed Contents 1 Gene 1 1 Locus 2 mRNA 2 1 Alternative splicing 3 Protein 3 1 General properties 3 2 Aliases 3 3 Composition 3 4 Post translational modifications 3 5 Interactions 3 6 Subcellular localization 3 7 Structure 3 8 Expression 4 Homology 4 1 Paralogs 4 2 Orthologs 4 3 Molecular Evolution 5 ReferencesGene editLocus editThis gene is located on chromosome 1 at 1q31 1 3 It is encoded on the plus strand of DNA spanning from 186 344 406 to 186 390 514 nbsp Diagram of C1orf27 and nearby genes on human chromosome 1 3 mRNA editAlternative splicing edit There appear to be four isoforms due to splicing 4 Two of those are truncated on the 3 end of the protein from 266aa and 396aa Additional location of alternative splice sites are from 79aa to 102aa and 246aa to 260aa Protein editGeneral properties edit The primary encoded protein of C1orf27 consists of 454 amino acid residues and is 3926 base pairs long 1 It consists of 14 total exons The predicted molecular weight of the primary unmodified protein is approximately 51 1 kdal Aliases edit As with many other genes there are some common aliases found with this gene citation needed Those aliases are Lymphocyte Activation Gene 1 LAG1 Interacting Protein Transparent Testa Glabra 1 TTG1 and Odorant Response Abnormal 4 ODR4 The most common alias for C1orf27 is ODR4 and this is what most readily appears when searching the gene Composition edit Computational analysis revealed the most abundant amino acid to be leucine at 10 1 of the total protein 5 The second most abundant was serine which contributes to 8 6 of the total protein Glutamic acid was third most abundant and contributes to 7 7 of the protein This analysis also revealed that the protein appears to be deficient in tryptophan as it only contributes to 1 1 of the protein 5 Based on the distribution of other amino acid types there were five high scoring hydrophobic segments There were also two transmembrane domains located at 82 98aa and 432 449aa Post translational modifications edit Interactor Number of Predicted Sites Function N myristoylation8 Key components of signaling pathways and typically promotes membrane binding essential for protein localization and or biological function 6 N glycosylation 4 Increase protein stability by decreasing protein dynamics 7 Protein Kinase C Phosphorylation7Enzymatic activity regulation 8 Casein Kinase II Phosphorylation 7 Epidermal growth factor role 9 Tyrosine Kinase Phosphorylation 2 Alterations to the structural conformation 10 cAMP and cGMP Dependent Phosphorylation 2 Coordination of the active site conformation and enzymatic activity C1orf27 is predicted to undergo multiple post translational modifications such as glycosylation myristoylation and phosphorylation 11 Interactions edit There were eight interactions identified by Mentha 12 The first one was UFSP2 which hydrolyzes the peptide bond at the C term gly of UFM1 a ubiquitin like modifier protein bound to a number of target proteins The second one was HSCB which acts as a co chaperone in iron sulfur cluster assembly in mitochondria The third was GRB2 which is an adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway The fourth was CYLD which is a protease that cleaves Lys 63 linked polyubiquitin chains controls regulation of cell survival proliferation and differentiation and is required for normal cell cycle progress The fifth was ATM which activates checkpoint signaling upon double strand breaks apoptosis and genotoxic stress The sixth was FAM177A1 the function of which is unknown The last two were THID2 and Q81kP6 which are both in bacillus anthracis Subcellular localization edit The c1orf27 protein is likely cytoplasmic 13 This was found with 55 5 reliability The K NN prediction was k 9 23 and the protein was found to be 55 6 cytoplasmic 11 1 mitochondrial 11 1 vacuolar 11 1 cytoskeletal and 11 1 golgi Structure edit nbsp Predicted tertiary structure of c1orf27 14 Alpha helices predicted in the c1orf27 protein are colored blue in the above picture Beta sheets are pictured by the red arrows Random coils are the purple strands between structures Expression edit nbsp Expression of C1orf27 in human tissues 15 Overall expression of c1orf27 seems to be ubiquitous 16 Highest expression body sites gt 50 TPM were bladder bone marrow kidney liver pancreas parathyroid and vascular Highest expression health sites gt 50 TPM were adrenal tumors cervical tumors and liver tumors While both of these observations had relatively high TPM scores there was still relatively low occurrence This validates the assumption that expression is ubiquitous There was moderate expression gt 25 TPM in the human fetus and expression increased with age 16 Expression was completely absent in the ears esophagus lymph nerve salivary glands thyroid tonsils and umbilical cord There was no expression in bladder carcinoma despite expression being elevated in the bladder itself There was high expression in endothelial cells and neuronal cells but was undetectable in glial cells and neuropil cells Expression was also localized to the nucleoplasm and plasma membrane in humans but is localized to the cytosol in mice Homology editParalogs edit There were no paralogs of C1orf27 identified in the human genome 4 Orthologs edit There were orthologs identified in most animals for which there were complete genome data 4 The most distant yet still relevant orthologs identified were invertebrates from phylum Cnidaria Molecular Evolution edit nbsp Rate of sequence divergence for C1orf27 in comparison to Fibrinogen and Cytochrome C in orthologs The m value or number of corrected amino acid changes per 100 residues for the C1orf27 gene was graphed against the species divergence in millions of years When compared to divergence graphs of fibrinogen and cytochrome C it was determined that this gene closely resembles the evolutionary pattern observed in fibrinogen suggesting a more rapid rate of evolution M values for C1orf27 were calculated using the percentage of identity when compared to humans observed in the mRNA sequences of the orthologs using the formula derived from the Molecular Clock Hypothesis References edit a b Homo sapiens odr 4 GPCR localization factor homolog ODR4 transcript Nucleotide NCBI www ncbi nlm nih gov Retrieved 2018 02 05 Tissue expression of C1orf27 Summary The Human Protein Atlas www proteinatlas org Retrieved 2018 02 05 a b ODR4 odr 4 GPCR localization factor homolog Homo sapiens human Gene NCBI www ncbi nlm nih gov Retrieved 2018 02 19 a b c Protein BLAST search protein databases using a protein query blast ncbi nlm nih gov Retrieved 2018 02 25 a b EMBL EBI SAPS lt Sequence Statistics lt EMBL EBI www ebi ac uk Retrieved 2018 04 22 Wright MH Heal WP Mann DJ Tate EW March 2010 Protein myristoylation in health and disease Journal of Chemical Biology 3 1 19 35 doi 10 1007 s12154 009 0032 8 PMC 2816741 PMID 19898886 Proteomics Post translational Modification Glycosylation Wikibooks open books for an open world en wikibooks org Retrieved 2018 05 06 Robles Flores M Melendez L Garcia W Mendoza Hernandez G Lam TT Castaneda Patlan C Gonzalez Aguilar H May 2008 Posttranslational modifications on protein kinase c isozymes Effects of epinephrine and phorbol esters Biochimica et Biophysica Acta BBA Molecular Cell Research 1783 5 695 712 doi 10 1016 j bbamcr 2007 07 011 PMID 18295358 Siomi MC Higashijima K Ishizuka A Siomi H December 2002 Casein kinase II phosphorylates the fragile X mental retardation protein and modulates its biological properties Molecular and Cellular Biology 22 24 8438 47 doi 10 1128 MCB 22 24 8438 8447 2002 PMC 139871 PMID 12446764 Rahimi N Costello CE January 2015 Emerging roles of post translational modifications in signal transduction and angiogenesis Proteomics 15 2 3 300 9 doi 10 1002 pmic 201400183 PMC 4297243 PMID 25161153 ExPASy PROSITE prosite expasy org Retrieved 2018 04 22 mentha the interactome browser mentha uniroma2 it Retrieved 2018 04 22 PSORT II Prediction psort hgc jp Retrieved 2018 04 22 Kelley Lawrence PHYRE2 Protein Fold Recognition Server www sbg bio ic ac uk Retrieved 2018 05 06 GDS1402 NM 017847 1 PROBE1 www ncbi nlm nih gov Retrieved 2018 05 06 a b Group Schuler EST Profile Hs 371210 www ncbi nlm nih gov Retrieved 2018 05 06 Retrieved from https en wikipedia org w index php title C1orf27 amp oldid 1223674025, wikipedia, wiki, book, books, library,

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