Sergei Winogradsky

Sergei Nikolaevich Winogradsky ForMemRS^[1] (Russian: Сергей Николаевич Виноградский; Ukrainian: Сергій Миколайович Виноградський; 13 September [O.S. 1 September] 1856, Kyiv – 24 February 1953, Brie-Comte-Robert)^[2], also published under the name Sergius Winogradsky,^[3] was a Russian^[4][5] microbiologist, ecologist and soil scientist who pioneered the cycle-of-life concept.^[6][7] Winogradsky discovered the first known form of lithotrophy during his research with Beggiatoa in 1887. He reported that Beggiatoa oxidized hydrogen sulfide (H₂S) as an energy source and formed intracellular sulfur droplets.^[3] This research provided the first example of lithotrophy, but not autotrophy. Born in the capital of present-day Ukraine, his legacy is also celebrated by this nation.^[8]

Sergei Winogradsky
Born	13 September 1856 (1856-09-13) Kyiv, Kiev Governorate, Russian Empire
Died	24 February 1953 (1953-02-25) (aged 96) Brie-Comte-Robert, France
Alma mater	Saint Petersburg Imperial University
Known for	Nitrogen cycle Chemoautotrophy Sulfur-oxidizing bacteria
Awards	Leeuwenhoek Medal (1935) Fellow of the Royal Society^[1]
Scientific career
Fields	Microbiology
Institutions	Saint Petersburg Imperial University University of Straßburg Institute of Experimental Medicine Pasteur Institute
Signature

His research on nitrifying bacteria would report the first known form of chemoautotrophy, showing how a lithotroph fixes carbon dioxide (CO₂) to make organic compounds.^[9]

He is best known in school science as the inventor of the Winogradsky column technique for the study of sediment microbes.

Biography

Winogradsky was born in Kyiv, Russian Empire to a family of wealthy lawyers. Among his paternal ancestors were Cossack atamans, and on the maternal side he was linked to the Skoropadsky family.^[10] In his youth Winogradsky was "strictly devoted to the Orthodox faith", though he later became irreligious.^[11]

After graduating from the 2nd Kiev Gymnasium in 1873, he began studying law, but he entered the Imperial Conservatoire of Music in St Petersburg in 1875 to study piano.^[1] However, after two years of music training, he entered the Saint Petersburg Imperial University in 1877 to study chemistry under Nikolai Menshutkin and botany under Andrei Famintsyn,^[1] receiving his degree in 1881 and staying on for a master's in botany, which he received in 1884. In 1885, he moved to the University of Straßburg to work under the renowned botanist Anton de Bary, subsequently becoming renowned for his work on sulfur bacteria.

In 1888, after de Bary's death, he relocated to Zurich, where he began investigation into the process of nitrification, identifying the genera Nitrosomonas and Nitrosococcus, which oxidizes ammonium to nitrite, and Nitrobacter, which oxidizes nitrite to nitrate.^[12]

He returned to St. Petersburg for the period 1891–1905, obtaining his doctoral degree in 1902 and from then on heading the division of general microbiology of the Institute of Experimental Medicine. During this period, he identified the obligate anaerobe Clostridium pasteurianum, which is capable of fixing atmospheric nitrogen. In St. Petersburg he trained Vasily Omelianski, who popularized Winogradskys concepts and methodology in the Soviet Union during the next decades.^[13]

In 1901, he was elected an honorary member of the Moscow Society of Naturalists and, in 1902, a corresponding member of the French Academy of Sciences. In 1905, due to ill health, the scientist left the institute and moved from St. Petersburg to the town of Gorodok in Podolia, where from 1892 he owned a huge estate. In fact, while working as the director of the Institute of Experimental Medicine, Winogradsky renounced his salary, which was transferred to a special account, and then used these funds to build a room for a scientific library, the director of which lived on the income from the estate, where agricultural work was carried out.^{[citation needed]}

In Gorodok Winogradsky addressed the problems of agriculture and soil science. He introduced new management methods, bought the best varieties of seeds, plants, and livestock, and advanced technology. His estate became one of the richest and most successful in Podolia, and remained profitable even during the First World War, falling under Austro-Hungarian occupation.^{[citation needed]}

He retired from active scientific work in 1905, dividing his time between his private estate in Gorodok and Switzerland. After the revolution of 1917, Winogradsky went first to Switzerland and then to Belgrade. In 1922, he accepted an invitation to head the Pasteur Institute's division of agricultural bacteriology at an experimental station at Brie-Comte-Robert, France, about 30 km from Paris. During this period, he worked on a number of topics, among them iron bacteria, nitrifying bacteria, nitrogen fixation by Azotobacter, cellulose-decomposing bacteria, and culture methods for soil microorganisms. In 1923 Winogradsky became an honorary member of the Russian Academy of Sciences despite his emigration. He retired from active life in 1940 and died in Brie-Comte-Robert in 1953.

Discoveries

Winogradsky discovered various biogeochemical cycles and parts of these cycles. These discoveries include

• His work on bacterial sulfide oxidation for which he first became renowned, including the first known form of lithotrophy (in Beggiatoa).
• His work on the Nitrogen cycle including
  • The identification of the obligate anaerobe Clostridium pasteurianum is a free living microbe capable of fixing atmospheric nitrogen and not living in legume root nodules.
  • Chemosynthesis – his most noted discovery
  • The Winogradsky column

 

Nitrogen cycle

Chemosynthesis

Winogradsky is best known for discovering chemoautotrophy, which soon became popularly known as chemosynthesis, the process by which organisms derive energy from a number of different inorganic compounds and obtain carbon in the form of carbon dioxide. Previously, it was believed that autotrophs obtained their energy solely from light, not from reactions of inorganic compounds. With the discovery of organisms that oxidized inorganic compounds such as hydrogen sulfide and ammonium as energy sources, autotrophs could be divided into two groups: photoautotrophs and chemoautotrophs. Winogradsky was one of the first researchers to attempt to understand microorganisms outside of the medical context, making him among the first students of microbial ecology and environmental microbiology.

The Winogradsky column remains an important display of chemoautotrophy and microbial ecology, demonstrated in microbiology lectures around the world.^[14]

Memorials

• The Institute of Microbiology of the Russian Academy of Sciences bears Winogradsky's name since 2003.
• In 2012, a bust of the scientist was unveiled on the grounds of his former estate in Horodok, Khmelnytskyi Oblast, Ukraine.^[10]
• In Ukraine, the study and popularization of the life and activities of Sergey Winogradsky are promoted by the Winogradsky Club, whose centre is located in the Horodok Museum of Local History (G-MUSEUM). One of the museum's exhibitions is a reconstruction of Winogradsky's laboratory in Brie-Comte-Robert including a wax figure of the scientist.

Representations of Winogradsky in Horodok

•  
  Bust of Winogradsky
•  
  Recreation of Winogradsky's laboratory at the G-MUSEUM

See also

• Hermann Hellriegel
• Martinus Beijerinck

Further reading

• Moshynets, O. (April 2013). "From Winogradsky's column to contemporary research using bacterial microcosms.". . Harris, C.C. (eds.). Nova. pp. 1–27. Archived from the original on 2016-02-01. Retrieved 2016-01-26.
• Ackert, Lloyd. Sergei Vinogradskii and the Cycle of Life: From the Thermodynamics of Life to Ecological Microbiology, 1850-1950. Vol. 34.; Dordrecht; London: Springer, 2013.
• Ackert, L. (2006). "The Role of Microbes in Agriculture: Sergei Vinogradskii's Discovery and Investigation of Chemosynthesis, 1880–1910". Journal of the History of Biology. 39 (2): 373–406. doi:10.1007/s10739-006-0008-2. S2CID 84400593.
• Ackert, L. (2007). "The 'Cycle of Life' in Ecology: Sergei Vinogradskii's Soil Microbiology, 1885–1940". Journal of the History of Biology. 40: 109–145. doi:10.1007/s10739-006-9104-6. S2CID 128410978.
• Waksman, S. A. (1946). "Sergei Nikolaevitch Winogradsky: The study of a great bacteriologist". Soil Science. 62: 197–226. doi:10.1097/00010694-194609000-00001. S2CID 94545224.

References

1. Thornton, H. G. (1953). "Sergei Nicholaevitch Winogradsky. 1856-1953". Biographical Memoirs of Fellows of the Royal Society. 8 (22): 635–644. doi:10.1098/rsbm.1953.0022. JSTOR 769234.
2. "Виноградський Сергій Миколайович". ukrainci.top (in Ukrainian). Retrieved 2024-02-12.
3. Winogradsky, Sergius (1887). "Ueber Schwefelbacterien". Botanische Zeitung (45): 489–512.
4. "Sergei Winogradsky".
5. "Sergey Nikolayevich Winogradsky | Microbiologist, Ecology, Soil Science | Britannica".
6. Waksman, S. A. (1953). "Sergei Nikolaevitch Winogradsky: 1856-1953". Science. 118 (3054): 36–37. Bibcode:1953Sci...118...36W. doi:10.1126/science.118.3054.36. PMID 13076173.
7. Dworkin, M. (2012). Gutnick, David (ed.). "Sergei Winogradsky: A founder of modern microbiology and the first microbial ecologist". FEMS Microbiology Reviews. 36 (2): 364–379. doi:10.1111/j.1574-6976.2011.00299.x. PMID 22092289.
8. Kryvyi, P. A.; Gumeniuk, G. L.; Bratchykova, Yu V. (2022-10-24). "Serhiy Winogradsky – a great Ukrainian". Infusion & Chemotherapy (3): 57–64. doi:10.32902/2663-0338-2022-3-57-64. ISSN 2709-0957. S2CID 253131734.
9. Dworkin, Martin; Falkow, Stanley (2006). The Prokaryotes: A Handbook on the Biology of Bacteria: Proteobacteria: Gamma Subclass (3rd ed.). Springer. p. 784. doi:10.1007/0-387-30746-X_27. ISBN 978-0-387-25496-8.
10. Шевелєва, Мар'яна (2023-09-01). "Сергій Виноградський: біля витоків концепції біосфери". Український інтерес. Retrieved 2024-02-12.
11. Waksman, Selman Abraham. 1953. Sergei N. Winogradsky: His Life and Work: The Story of a Great Bacteriologist. Rutgers University Press. p. 4
12. Winogradsky, M. S. (1890). "Recherches sur les organisms de la nitrification". Ann. Inst. Pasteur. 4: 213–231.
13. Waksman, Selman A. (1928). "Professor V. L. Omeliansky". Soil Science. 26 (4): 255–256. Bibcode:1928SoilS..26..255W. doi:10.1097/00010694-192810000-00001. ISSN 0038-075X.
14. Madigan, Michael T. (2012). Brock biology of microorganisms (13th ed.). San Francisco: Benjamin Cummings. ISBN 9780321649638.

External links

• Sergei Winogradsky at Cycle of Life website including images.
• page Winogradsky Club
• official website G-Museum