Lithium acetate is used in the laboratory as buffer for gel electrophoresis of DNA and RNA. It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer (5-30V/cm as compared to 5-10V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected when using lithium acetate gels.
Lithium boric acid or sodium boric acid are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA (less than 500 bp) due to the higher resolution of borate-based buffers in this size range as compared to acetate buffers.
Lithium acetate is also used to permeabilize the cell wall of yeast for use in DNA transformation. It is believed that the beneficial effect of LiOAc is caused by its chaotropic effect; denaturing DNA, RNA and proteins.[2]
Referencesedit
^ Lide, David R. (1998). Handbook of Chemistry and Physics (87 ed.). Boca Raton, FL: CRC Press. p. 465. ISBN0-8493-0594-2.
^Norcum, MT (Aug 15, 1991). "Structural analysis of the high molecular mass aminoacyl-tRNA synthetase complex. Effects of neutral salts and detergents". The Journal of Biological Chemistry. 266 (23): 15398–405. doi:10.1016/S0021-9258(18)98629-1. PMID 1651330.
Brody, JR; Kern, SE (2004). "History and principles of conductive media for standard DNA electrophoresis". Analytical Biochemistry. 333 (1): 1–13. doi:10.1016/j.ab.2004.05.054. PMID 15351274.
April 11, 2024
lithium, acetate, ch3cooli, salt, lithium, acetic, acid, often, abbreviated, lioac, namespreferred, iupac, name, identifierscas, number, anhydrous, y6108, dihydrate, model, jsmol, interactive, imagechebi, chebi, chebi, 63045, nchemspider, 10562, necha, infocar. Lithium acetate CH3COOLi is a salt of lithium and acetic acid It is often abbreviated as LiOAc Lithium acetate NamesPreferred IUPAC name Lithium acetateIdentifiersCAS Number 546 89 4 anhydrous Y6108 17 4 dihydrate Y3D model JSmol Interactive imageChEBI CHEBI CHEBI 63045 NChemSpider 10562 NECHA InfoCard 100 008 105EC Number 208 914 3KEGG D08134 NMeSH C488804PubChem CID 3474584RTECS number AI545000UNII H4278O5FLV YCompTox Dashboard EPA DTXSID00883436InChI InChI 1S C2H4O2 Li c1 2 3 4 h1H3 H 3 4 q 1 p 1 NKey XIXADJRWDQXREU UHFFFAOYSA M NInChI 1 C2H4O2 Li c1 2 3 4 h1H3 H 3 4 q 1 p 1Key XIXADJRWDQXREU REWHXWOFAXSMILES Li CC O O PropertiesChemical formula C 2H 3Li O 2Molar mass 65 98 g mol 1Appearance crystalDensity 1 26 g cm3Melting point 286 C 547 F 559 K Solubility in water 45 0 g 100 mL 1 Magnetic susceptibility x 34 0 10 6 cm3 molHazardsOccupational safety and health OHS OSH Main hazards toxicNFPA 704 fire diamond 200Lethal dose or concentration LD LC LD50 median dose 500 mg kg oral mouse Safety data sheet SDS External MSDSExcept where otherwise noted data are given for materials in their standard state at 25 C 77 F 100 kPa N verify what is Y N Infobox referencesUses editLithium acetate is used in the laboratory as buffer for gel electrophoresis of DNA and RNA It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer 5 30V cm as compared to 5 10V cm At a given voltage the heat generation and thus the gel temperature is much lower than with TAE buffers therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time Downstream applications such as isolation of DNA from a gel slice or Southern blot analysis work as expected when using lithium acetate gels Lithium boric acid or sodium boric acid are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA less than 500 bp due to the higher resolution of borate based buffers in this size range as compared to acetate buffers Lithium acetate is also used to permeabilize the cell wall of yeast for use in DNA transformation It is believed that the beneficial effect of LiOAc is caused by its chaotropic effect denaturing DNA RNA and proteins 2 References edit Lide David R 1998 Handbook of Chemistry and Physics 87 ed Boca Raton FL CRC Press p 465 ISBN 0 8493 0594 2 Norcum MT Aug 15 1991 Structural analysis of the high molecular mass aminoacyl tRNA synthetase complex Effects of neutral salts and detergents The Journal of Biological Chemistry 266 23 15398 405 doi 10 1016 S0021 9258 18 98629 1 PMID 1651330 Brody JR Kern SE 2004 History and principles of conductive media for standard DNA electrophoresis Analytical Biochemistry 333 1 1 13 doi 10 1016 j ab 2004 05 054 PMID 15351274 Retrieved from https en wikipedia org w index php title Lithium acetate amp oldid 1137910643, wikipedia, wiki, book, books, library,
