This gene is a member of the glutathione peroxidase family encoding a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract.[7]
The antioxidant enzyme glutathione peroxidase 2 (Gpx2) is one out of eight known glutathione peroxidases (Gpx1-8) in humans. Mammalian Gpx1, GPx2 (this protein), Gpx3, and Gpx4 have been shown to be selenium-containing enzymes, whereas Gpx6 is a selenoprotein in humans with cysteine-containing homologues in rodents. In selenoproteins, the 21st amino acid selenocysteine is inserted in the nascent polypeptide chain during the process of translational recoding of the UGA stop codon.
Referencesedit
^ abcGRCh38: Ensembl release 89: ENSG00000176153 – Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000042808 – Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Chu FF, Doroshow JH, Esworthy RS (Mar 1993). "Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSHPx-GI". J Biol Chem. 268 (4): 2571–6. doi:10.1016/S0021-9258(18)53812-6. PMID 8428933.
^Chu FF (Feb 1994). "The human glutathione peroxidase genes GPX2, GPX3, and GPX4 map to chromosomes 14, 5, and 19, respectively". Cytogenet Cell Genet. 66 (2): 96–8. doi:10.1159/000133675. PMID 8287691.
Akasaka M, Mizoguchi J, Takahashi K (1990). "A human cDNA sequence of a novel glutathione peroxidase-related protein". Nucleic Acids Res. 18 (15): 4619. doi:10.1093/nar/18.15.4619. PMC331311. PMID 2388849.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Chu FF, Rohan de Silva HA, Esworthy RS, et al. (1997). "Polymorphism and chromosomal localization of the GI-form of human glutathione peroxidase (GPX2) on 14q24.1 by in situ hybridization". Genomics. 32 (2): 272–6. doi:10.1006/geno.1996.0115. PMID 8833155.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Opalenik SR, Ding Q, Mallery SR, Thompson JA (1998). "Glutathione depletion associated with the HIV-1 TAT protein mediates the extracellular appearance of acidic fibroblast growth factor". Arch. Biochem. Biophys. 351 (1): 17–26. doi:10.1006/abbi.1997.0566. PMID 9501919.
Choi J, Liu RM, Kundu RK, et al. (2000). "Molecular mechanism of decreased glutathione content in human immunodeficiency virus type 1 Tat-transgenic mice". J. Biol. Chem. 275 (5): 3693–8. doi:10.1074/jbc.275.5.3693. PMID 10652368.
Kelner MJ, Bagnell RD, Montoya MA, Lanham KA (2000). "Structural organization of the human gastrointestinal glutathione peroxidase (GPX2) promoter and 3'-nontranscribed region: transcriptional response to exogenous redox agents". Gene. 248 (1–2): 109–16. doi:10.1016/S0378-1119(00)00137-2. PMID 10806356.
Richard MJ, Guiraud P, Didier C, et al. (2001). "Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a mechanism independent of cellular selenium uptake: consequences on cellular resistance to UV-A radiation". Arch. Biochem. Biophys. 386 (2): 213–20. doi:10.1006/abbi.2000.2197. PMID 11368344.
Komatsu H, Okayasu I, Mitomi H, et al. (2001). "Immunohistochemical detection of human gastrointestinal glutathione peroxidase in normal tissues and cultured cells with novel mouse monoclonal antibodies". J. Histochem. Cytochem. 49 (6): 759–66. doi:10.1177/002215540104900609. PMID 11373322.
Florian S, Wingler K, Schmehl K, et al. (2002). "Cellular and subcellular localization of gastrointestinal glutathione peroxidase in normal and malignant human intestinal tissue". Free Radic. Res. 35 (6): 655–63. doi:10.1080/10715760100301181. PMID 11811519. S2CID 22845933.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC139241. PMID 12477932.
Müller C, Wingler K, Brigelius-Flohé R (2003). "3'UTRs of glutathione peroxidases differentially affect selenium-dependent mRNA stability and selenocysteine incorporation efficiency". Biol. Chem. 384 (1): 11–8. doi:10.1515/BC.2003.002. PMID 12674495. S2CID 24693834.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC528928. PMID 15489334.
Price TO, Ercal N, Nakaoke R, Banks WA (2005). "HIV-1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells". Brain Res. 1045 (1–2): 57–63. doi:10.1016/j.brainres.2005.03.031. PMID 15910762. S2CID 7362454.
Banning A, Deubel S, Kluth D, et al. (2005). "The GI-GPx gene is a target for Nrf2". Mol. Cell. Biol. 25 (12): 4914–23. doi:10.1128/MCB.25.12.4914-4923.2005. PMC1140597. PMID 15923610.
Yan W, Chen X (2006). "GPX2, a direct target of p63, inhibits oxidative stress-induced apoptosis in a p53-dependent manner". J. Biol. Chem. 281 (12): 7856–62. doi:10.1074/jbc.M512655200. PMID 16446369.
Singh A, Rangasamy T, Thimmulappa RK, et al. (2007). "Glutathione peroxidase 2, the major cigarette smoke-inducible isoform of GPX in lungs, is regulated by Nrf2". Am. J. Respir. Cell Mol. Biol. 35 (6): 639–50. doi:10.1165/rcmb.2005-0325OC. PMC2643293. PMID 16794261.
gpx2, gene, glutathione, peroxidase, enzyme, that, humans, encoded, gpx2, gene, gpx2available, structurespdbortholog, search, pdbe, rcsblist, codes2he3identifiersaliasesgpx2, gprp, gprp, gshpx, gshpx, glutathione, peroxidase, 2external, idsomim, 138319, 106609. Glutathione peroxidase 2 is an enzyme that in humans is encoded by the GPX2 gene 5 6 7 GPX2Available structuresPDBOrtholog search PDBe RCSBList of PDB id codes2HE3IdentifiersAliasesGPX2 GI GPx GPRP GPRP 2 GPx 2 GPx GI GSHPX GI GSHPx 2 glutathione peroxidase 2External IDsOMIM 138319 MGI 106609 HomoloGene 20479 GeneCards GPX2Gene location Human Chr Chromosome 14 human 1 Band14q23 3Start64 939 152 bp 1 End64 942 746 bp 1 Gene location Mouse Chr Chromosome 12 mouse 2 Band12 C3 12 33 73 cMStart76 839 107 bp 2 End76 842 273 bp 2 RNA expression patternBgeeHumanMouse ortholog Top expressed ingallbladderrectumpancreatic ductal cellislet of Langerhansright lobe of liverappendixurinary bladdergumsduodenumepithelium of esophagusTop expressed incrypt of lieberkuhn of small intestineesophaguslarge intestinecolonduodenumjejunumleft colonmucous cell of stomachepithelium of stomachsuperior surface of tongueMore reference expression dataBioGPSMore reference expression dataGene ontologyMolecular functionelectron transfer activity oxidoreductase activity glutathione peroxidase activity peroxidase activityCellular componentcytoplasm cytosolBiological processresponse to oxidative stress cellular oxidant detoxification cellular response to oxidative stress electron transport chainSources Amigo QuickGOOrthologsSpeciesHumanMouseEntrez287714776EnsemblENSG00000176153ENSMUSG00000042808UniProtP18283Q9JHC0RefSeq mRNA NM 002083NM 030677RefSeq protein NP 002074NP 109602Location UCSC Chr 14 64 94 64 94 MbChr 12 76 84 76 84 MbPubMed search 3 4 WikidataView Edit HumanView Edit Mouse This gene is a member of the glutathione peroxidase family encoding a selenium dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione dependent hydrogen peroxide reducing activity in the epithelium of the gastrointestinal tract Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract 7 The antioxidant enzyme glutathione peroxidase 2 Gpx2 is one out of eight known glutathione peroxidases Gpx1 8 in humans Mammalian Gpx1 GPx2 this protein Gpx3 and Gpx4 have been shown to be selenium containing enzymes whereas Gpx6 is a selenoprotein in humans with cysteine containing homologues in rodents In selenoproteins the 21st amino acid selenocysteine is inserted in the nascent polypeptide chain during the process of translational recoding of the UGA stop codon References edit a b c GRCh38 Ensembl release 89 ENSG00000176153 Ensembl May 2017 a b c GRCm38 Ensembl release 89 ENSMUSG00000042808 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Mouse PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Chu FF Doroshow JH Esworthy RS Mar 1993 Expression characterization and tissue distribution of a new cellular selenium dependent glutathione peroxidase GSHPx GI J Biol Chem 268 4 2571 6 doi 10 1016 S0021 9258 18 53812 6 PMID 8428933 Chu FF Feb 1994 The human glutathione peroxidase genes GPX2 GPX3 and GPX4 map to chromosomes 14 5 and 19 respectively Cytogenet Cell Genet 66 2 96 8 doi 10 1159 000133675 PMID 8287691 a b Entrez Gene GPX2 glutathione peroxidase 2 gastrointestinal Further reading editAkasaka M Mizoguchi J Takahashi K 1990 A human cDNA sequence of a novel glutathione peroxidase related protein Nucleic Acids Res 18 15 4619 doi 10 1093 nar 18 15 4619 PMC 331311 PMID 2388849 Maruyama K Sugano S 1994 Oligo capping a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides Gene 138 1 2 171 4 doi 10 1016 0378 1119 94 90802 8 PMID 8125298 Chu FF Rohan de Silva HA Esworthy RS et al 1997 Polymorphism and chromosomal localization of the GI form of human glutathione peroxidase GPX2 on 14q24 1 by in situ hybridization Genomics 32 2 272 6 doi 10 1006 geno 1996 0115 PMID 8833155 Suzuki Y Yoshitomo Nakagawa K Maruyama K et al 1997 Construction and characterization of a full length enriched and a 5 end enriched cDNA library Gene 200 1 2 149 56 doi 10 1016 S0378 1119 97 00411 3 PMID 9373149 Opalenik SR Ding Q Mallery SR Thompson JA 1998 Glutathione depletion associated with the HIV 1 TAT protein mediates the extracellular appearance of acidic fibroblast growth factor Arch Biochem Biophys 351 1 17 26 doi 10 1006 abbi 1997 0566 PMID 9501919 Choi J Liu RM Kundu RK et al 2000 Molecular mechanism of decreased glutathione content in human immunodeficiency virus type 1 Tat transgenic mice J Biol Chem 275 5 3693 8 doi 10 1074 jbc 275 5 3693 PMID 10652368 Kelner MJ Bagnell RD Montoya MA Lanham KA 2000 Structural organization of the human gastrointestinal glutathione peroxidase GPX2 promoter and 3 nontranscribed region transcriptional response to exogenous redox agents Gene 248 1 2 109 16 doi 10 1016 S0378 1119 00 00137 2 PMID 10806356 Richard MJ Guiraud P Didier C et al 2001 Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a mechanism independent of cellular selenium uptake consequences on cellular resistance to UV A radiation Arch Biochem Biophys 386 2 213 20 doi 10 1006 abbi 2000 2197 PMID 11368344 Komatsu H Okayasu I Mitomi H et al 2001 Immunohistochemical detection of human gastrointestinal glutathione peroxidase in normal tissues and cultured cells with novel mouse monoclonal antibodies J Histochem Cytochem 49 6 759 66 doi 10 1177 002215540104900609 PMID 11373322 Florian S Wingler K Schmehl K et al 2002 Cellular and subcellular localization of gastrointestinal glutathione peroxidase in normal and malignant human intestinal tissue Free Radic Res 35 6 655 63 doi 10 1080 10715760100301181 PMID 11811519 S2CID 22845933 Strausberg RL Feingold EA Grouse LH et al 2003 Generation and initial analysis of more than 15 000 full length human and mouse cDNA sequences Proc Natl Acad Sci U S A 99 26 16899 903 Bibcode 2002PNAS 9916899M doi 10 1073 pnas 242603899 PMC 139241 PMID 12477932 Muller C Wingler K Brigelius Flohe R 2003 3 UTRs of glutathione peroxidases differentially affect selenium dependent mRNA stability and selenocysteine incorporation efficiency Biol Chem 384 1 11 8 doi 10 1515 BC 2003 002 PMID 12674495 S2CID 24693834 Esworthy RS Binder SW Doroshow JH Chu FF 2003 Microflora trigger colitis in mice deficient in selenium dependent glutathione peroxidase and induce Gpx2 gene expression Biol Chem 384 4 597 607 doi 10 1515 BC 2003 067 PMID 12751789 S2CID 25131807 Gerhard DS Wagner L Feingold EA et al 2004 The status quality and expansion of the NIH full length cDNA project the Mammalian Gene Collection MGC Genome Res 14 10B 2121 7 doi 10 1101 gr 2596504 PMC 528928 PMID 15489334 Price TO Ercal N Nakaoke R Banks WA 2005 HIV 1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells Brain Res 1045 1 2 57 63 doi 10 1016 j brainres 2005 03 031 PMID 15910762 S2CID 7362454 Banning A Deubel S Kluth D et al 2005 The GI GPx gene is a target for Nrf2 Mol Cell Biol 25 12 4914 23 doi 10 1128 MCB 25 12 4914 4923 2005 PMC 1140597 PMID 15923610 Yan W Chen X 2006 GPX2 a direct target of p63 inhibits oxidative stress induced apoptosis in a p53 dependent manner J Biol Chem 281 12 7856 62 doi 10 1074 jbc M512655200 PMID 16446369 Singh A Rangasamy T Thimmulappa RK et al 2007 Glutathione peroxidase 2 the major cigarette smoke inducible isoform of GPX in lungs is regulated by Nrf2 Am J Respir Cell Mol Biol 35 6 639 50 doi 10 1165 rcmb 2005 0325OC PMC 2643293 PMID 16794261 Portal nbsp Biology nbsp This article on a gene on human chromosome 14 is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title GPX2 gene amp oldid 1142728560, wikipedia, wiki, book, books, library,
