Sabouraud agar or Sabouraud dextrose agar (SDA) is a type of agar growth medium containing peptones.[1] It is used to cultivate dermatophytes and other types of fungi, and can also grow filamentous bacteria such as Nocardia.[2][3][4] It has utility for research and clinical care.
It was created by, and is named after, Raymond Sabouraud in 1892. In 1977 the formulation was adjusted by Chester W. Emmons when the pH level was brought closer to the neutral range and the dextrose concentration lowered to support the growth of other microorganisms. The acidic pH (5.6) of traditional Sabouraud agar inhibits bacterial growth.[5] Peptones are complex digests and can be a source of variability in Sabouraud agar.[6]
Typical compositionedit
Sabouraud agar is commercially available and typically contains:[7]
Clinical laboratories can use this growth medium to diagnose and further speciate fungal infections, allowing medical professionals to provide appropriate treatment with antifungal medications. Histoplasma and other fungal causes of atypical pneumonia can be grown on this medium. Sabouraud agar used in combination with additional media, such as Inhibitory Mold Agar (IMA), improves identification of fungal clinical isolates.[8]
Referencesedit
^. Waksman Foundation For Microbiology. Archived from the original on 2008-10-06. Retrieved 2008-10-24.
^Sandven P, Lassen J (November 1999). "Importance of selective media for recovery of yeasts from clinical specimens". Journal of Clinical Microbiology. 37 (11): 3731–3732. doi:10.1128/JCM.37.11.3731-3732.1999. PMC85742. PMID 10523586.
^Guinea J, Peláez T, Alcalá L, Bouza E (December 2005). "Evaluation of Czapeck agar and Sabouraud dextrose agar for the culture of airborne Aspergillus conidia". Diagnostic Microbiology and Infectious Disease. 53 (4): 333–334. doi:10.1016/j.diagmicrobio.2005.07.002. PMID 16263232.
^(PDF). www.bd.com. Archived from the original (PDF) on 19 April 2009.
^Hare JM (9 December 2012). "15. Sabouraud agar for fungal growth". In Gupta VK, Tuohy MG, Ayyachamy M, Turner KM, O'Donovan A (eds.). Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology. Springer. p. 212. ISBN978-1-4614-2355-3.
^Odds FC (January 1991). "Sabouraud('s) agar". Journal of Medical and Veterinary Mycology. 29 (6): 355–359. doi:10.1080/02681219180000581. PMID 1815027.
^Scognamiglio T, Zinchuk R, Gumpeni P, Larone DH (May 2010). "Comparison of inhibitory mold agar to Sabouraud dextrose agar as a primary medium for isolation of fungi". Journal of Clinical Microbiology. 48 (5): 1924–1925. doi:10.1128/JCM.01814-09. PMC2863941. PMID 20220162.
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sabouraud, agar, sabouraud, dextrose, agar, type, agar, growth, medium, containing, peptones, used, cultivate, dermatophytes, other, types, fungi, also, grow, filamentous, bacteria, such, nocardia, utility, research, clinical, care, bottom, view, plate, with, . Sabouraud agar or Sabouraud dextrose agar SDA is a type of agar growth medium containing peptones 1 It is used to cultivate dermatophytes and other types of fungi and can also grow filamentous bacteria such as Nocardia 2 3 4 It has utility for research and clinical care Bottom view of a Sabouraud agar plate with a colony of Trichophyton rubrum var rodhaini Sporothrix schenckii in Sabouraud agar Candida albicans in Sabouraud agar Trichophyton terrestre in Sabouraud agar It was created by and is named after Raymond Sabouraud in 1892 In 1977 the formulation was adjusted by Chester W Emmons when the pH level was brought closer to the neutral range and the dextrose concentration lowered to support the growth of other microorganisms The acidic pH 5 6 of traditional Sabouraud agar inhibits bacterial growth 5 Peptones are complex digests and can be a source of variability in Sabouraud agar 6 Typical composition editSabouraud agar is commercially available and typically contains 7 40 g L dextrose 10 g L peptone 20 g L agar pH 5 6Medical use editClinical laboratories can use this growth medium to diagnose and further speciate fungal infections allowing medical professionals to provide appropriate treatment with antifungal medications Histoplasma and other fungal causes of atypical pneumonia can be grown on this medium Sabouraud agar used in combination with additional media such as Inhibitory Mold Agar IMA improves identification of fungal clinical isolates 8 References edit Omnipresence of Microorganisms in the Environment Waksman Foundation For Microbiology Archived from the original on 2008 10 06 Retrieved 2008 10 24 Sandven P Lassen J November 1999 Importance of selective media for recovery of yeasts from clinical specimens Journal of Clinical Microbiology 37 11 3731 3732 doi 10 1128 JCM 37 11 3731 3732 1999 PMC 85742 PMID 10523586 Guinea J Pelaez T Alcala L Bouza E December 2005 Evaluation of Czapeck agar and Sabouraud dextrose agar for the culture of airborne Aspergillus conidia Diagnostic Microbiology and Infectious Disease 53 4 333 334 doi 10 1016 j diagmicrobio 2005 07 002 PMID 16263232 About Modified Sabouraud Agar PDF www bd com Archived from the original PDF on 19 April 2009 Hare JM 9 December 2012 15 Sabouraud agar for fungal growth In Gupta VK Tuohy MG Ayyachamy M Turner KM O Donovan A eds Laboratory Protocols in Fungal Biology Current Methods in Fungal Biology Springer p 212 ISBN 978 1 4614 2355 3 Odds FC January 1991 Sabouraud s agar Journal of Medical and Veterinary Mycology 29 6 355 359 doi 10 1080 02681219180000581 PMID 1815027 Recipes University of Sydney 2004 Archived from the original on 14 December 2012 Scognamiglio T Zinchuk R Gumpeni P Larone DH May 2010 Comparison of inhibitory mold agar to Sabouraud dextrose agar as a primary medium for isolation of fungi Journal of Clinical Microbiology 48 5 1924 1925 doi 10 1128 JCM 01814 09 PMC 2863941 PMID 20220162 nbsp This microbiology related article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Sabouraud agar amp oldid 1179199275, wikipedia, wiki, book, books, library,
