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PIPES

April 11, 2024

This article is about the biochemical buffer PIPES. For other uses, see Pipe (disambiguation).

PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)) is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]

PIPES
Names
Preferred IUPAC name
2,2′-(Piperazine-1,4-diyl)di(ethane-1-sulfonic acid)
Other names
PIPES
Identifiers
  • 5625-37-6 Y
3D model (JSmol)
  • Interactive image
ChemSpider
  • 72022 N
ECHA InfoCard 100.024.598
  • 6992709
UNII
  • G502H79V6L Y
  • DTXSID8063965
  • InChI=1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) N
    Key: IHPYMWDTONKSCO-UHFFFAOYSA-N N
  • InChI=1/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
    Key: IHPYMWDTONKSCO-UHFFFAOYAG
  • C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Properties
C8H18N2O6S2
Molar mass 302.37
Appearance White powder
Melting point Decomposes above 300 °C
Boiling point Decomposes
1 g/L (100 °C)
Hazards
Occupational safety and health (OHS/OSH):
Main hazards
 Irritant
NFPA 704 (fire diamond)
Health 1: Exposure would cause irritation but only minor residual injury. E.g. turpentineFlammability 0: Will not burn. E.g. waterInstability 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogenSpecial hazards (white): no code
1
0
0
Safety data sheet (SDS) External MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N verify (what is YN ?)

Applications edit

PIPES has two pKa values. One pKa (6.76 at 25 °C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25 °C. The second pKa value is at 2.67 with a buffer range of from 1.5-3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.[5]

See also edit

References edit

  1. ^ Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry. 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
  2. ^ Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
  3. ^ Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
  4. ^ Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry. 33 (2): 110–8. doi:10.1177/33.2.3918095. PMID 3918095.
  5. ^ "Hopax Fine Chemicals - Biological buffers and their interactions with metal ions".


 

This biochemistry article is a stub. You can help Wikipedia by expanding it.

April 11, 2024
pipes, this, article, about, biochemical, buffer, other, uses, pipe, disambiguation, piperazine, ethanesulfonic, acid, frequently, used, buffering, agent, biochemistry, ethanesulfonic, acid, buffer, developed, good, 1960s, namespreferred, iupac, name, piperazi. This article is about the biochemical buffer PIPES For other uses see Pipe disambiguation PIPES piperazine N N bis 2 ethanesulfonic acid is a frequently used buffering agent in biochemistry It is an ethanesulfonic acid buffer developed by Good et al in the 1960s 1 PIPES NamesPreferred IUPAC name 2 2 Piperazine 1 4 diyl di ethane 1 sulfonic acid Other names PIPESIdentifiersCAS Number 5625 37 6 Y3D model JSmol Interactive imageChemSpider 72022 NECHA InfoCard 100 024 598PubChem CID 6992709UNII G502H79V6L YCompTox Dashboard EPA DTXSID8063965InChI InChI 1S C8H18N2O6S2 c11 17 12 13 7 5 9 1 2 10 4 3 9 6 8 18 14 15 16 h1 8H2 H 11 12 13 H 14 15 16 NKey IHPYMWDTONKSCO UHFFFAOYSA N NInChI 1 C8H18N2O6S2 c11 17 12 13 7 5 9 1 2 10 4 3 9 6 8 18 14 15 16 h1 8H2 H 11 12 13 H 14 15 16 Key IHPYMWDTONKSCO UHFFFAOYAGSMILES C1CN CCN1CCS O O O CCS O O OPropertiesChemical formula C8H18N2O6S2Molar mass 302 37Appearance White powderMelting point Decomposes above 300 CBoiling point DecomposesSolubility in water 1 g L 100 C HazardsOccupational safety and health OHS OSH Main hazards IrritantNFPA 704 fire diamond 100Safety data sheet SDS External MSDSExcept where otherwise noted data are given for materials in their standard state at 25 C 77 F 100 kPa N verify what is Y N Infobox referencesApplications editPIPES has two pKa values One pKa 6 76 at 25 C is near the physiological pH which makes it useful in cell culture work Its effective buffering range is 6 1 7 5 at 25 C The second pKa value is at 2 67 with a buffer range of from 1 5 3 5 PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues 2 3 Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer 4 It has a negligible capacity to bind divalent ions 5 See also editMOPS HEPES MES Tris Common buffer compounds used in biology Good s buffersReferences edit Good Norman E Winget G Douglas Winter Wilhelmina Connolly Thomas N Izawa Seikichi Singh Raizada M M 1966 Hydrogen Ion Buffers for Biological Research Biochemistry 5 2 467 77 doi 10 1021 bi00866a011 PMID 5942950 Salema R and Brando I J Submicr Cytol 9 79 1973 Schiff R I and Gennaro J F Scaning Electron Microsc 3 449 1979 Hardham A R 1985 Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi The influence of fixation on patterns of lectin binding Journal of Histochemistry 33 2 110 8 doi 10 1177 33 2 3918095 PMID 3918095 Hopax Fine Chemicals Biological buffers and their interactions with metal ions nbsp This biochemistry article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title PIPES amp oldid 1194234458, wikipedia, wiki, book, books, library,

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