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G. Marius Clore

March 05, 2023

G. Marius Clore MAE, FRSC, FRS is a British-born, Anglo-American molecular biophysicist and structural biologist. He was born in London, U.K. and is a dual U.S./U.K. Citizen.[1][2][3] He is a Member of the National Academy of Sciences,[4] a Fellow of the Royal Society,[5] a NIH Distinguished Investigator, and the Chief of the Molecular and Structural Biophysics Section in the Laboratory of Chemical Physics of the National Institute of Diabetes and Digestive and Kidney Diseases at the U.S. National Institutes of Health.[6][7] He is known for his foundational work in three-dimensional protein and nucleic acid structure determination by biomolecular NMR spectroscopy,[8] for advancing experimental approaches to the study of large macromolecules and their complexes by NMR,[9] and for developing NMR-based methods to study rare conformational states in protein-nucleic acid[10] and protein-protein[11] recognition.[12] Clore's discovery of previously undetectable, functionally significant, rare transient states of macromolecules has yielded fundamental new insights into the mechanisms of important biological processes, and in particular the significance of weak interactions and the mechanisms whereby the opposing constraints of speed and specificity are optimized. Further, Clore's work opens up a new era of pharmacology and drug design as it is now possible to target structures and conformations that have been heretofore unseen.[13]

G. Marius Clore

MAE FRSC FRS
Born
Gideon Marius Clore

1955[1]
London, U.K.
NationalityBritish
CitizenshipBritish, American
Alma materUniversity College London and University College Hospital Medical School, London, U.K.
Known forLaying the foundations for three-dimensional protein structure determination in solution by NMR, developing innovative approaches for extending NMR to larger and more complex systems, and using NMR to uncover invisible states of proteins
Awards•Member of the National Academy of Sciences
•Fellow of the Royal Society
•Fellow of the American Academy of Arts and Sciences
•Foreign Member of the Academia Europaea
•Royal Society of Chemistry Centenary Prize (2011)
•Biochemical Society Centenary Award (2013)
•Royal Society of Chemistry Khorana Prize (2021)
Scientific career
FieldsMolecular Biophysics, Nuclear Magnetic Resonance, Structural Biology, Chemistry
Institutions
Doctoral advisorSir Arnold Burgen FRS
Notable students
Influences
Influenced
Websitegmclore.org

Biography

Clore received his undergraduate degree with first class honours in biochemistry from University College London in 1976 and medical degree from UCL Medical School in 1979.[4] After completing house physician and house surgeon appointments at University College Hospital and St Charles' Hospital (part of the St. Mary's Hospital group), respectively, he was a member of the scientific staff of the Medical Research Council National Institute for Medical Research from 1980 to 1984. He received his PhD from the National Institute for Medical Research in Physical Biochemistry in 1982. He was awarded a joint Lister Institute Research Fellowship from the Lister Institute of Preventive Medicine which he held from 1982 to 1984 at the Medical Research Council.[14] In 1984 he joined the Max Planck Institute for Biochemistry in Martinsried, Germany, where he headed the Biological NMR department from 1984 to 1988.[1][2]

In 1988, Clore was recruited to the National Institutes of Health (NIH) Laboratory of Chemical Physics (National Institute of Diabetes and Digestive and Kidney Diseases) located in Bethesda, Maryland, U.S., where he interacted closely in the late 1980s and early 1990s with NIH colleagues Ad Bax, Angela Gronenborn and Dennis Torchia on the development of multidimensional heteronuclear NMR spectroscopy and a structural biology effort aimed at proteins involved in the pathogenesis of HIV/AIDS.[15] He has remained at the NIH ever since and is currently a NIH Distinguished Investigator and Chief of the Section on Molecular and Structural Biophysics at the NIH.[4] He is an elected Member of the United States National Academy of Sciences,[16] a Fellow of the Royal Society,[17] a Fellow of the American Academy of Arts and Sciences,[18][19] and a Foreign Member of the Academia Europaea (Biochemistry and Molecular Biology Section).[20] Clore's citation upon election to the Royal Society reads:

"Clore pioneered the development of NMR for determining three-dimensional structures of biological macromolecules and has consistently extended the frontiers of NMR to ever more complex systems. His work on the development of paramagnetic and other relaxation-based NMR experiments to detect and visualize transient, rare states of macromolecules, invisible to conventional structural and biophysical techniques, has shed unique insights into how macromolecules efficiently locate their binding partners, provided the first atomic view of the dynamic amyloid Aß assembly process from disordered peptides into protofibrils, and directly demonstrated that the apo state of the chaperonin GroEL possesses intrinsic foldase/unfoldase activities."[5]

Research

3D structure determination in solution by NMR

Clore played a pivotal role in the development of three- and four-dimensional NMR spectroscopy,[21] the use of residual dipolar couplings for structure determination,[22] the development of simulated annealing and restrained molecular dynamics for three-dimensional protein and nucleic acid structure determination,[23] the solution NMR structure determination of large protein complexes,[24] the development of the combined use of NMR and small-angle X-ray scattering in solution structure determination,[25] and the analysis and characterization of protein dynamics by NMR.[26] Clore's work on complexes of all the cytoplasmic components of the bacterial phosphotransferase system (PTS) led to significant insights into how signal transduction proteins recognize multiple, structurally dissimilar partners by generating similar binding surfaces from completely different structural elements and exploiting side chain conformational plasticity.[24] Clore is also one of the main authors of the very widely used XPLOR-NIH NMR structure determination program[27]

Detection and visualization of excited and sparsely-populated states

Clore's recent work has focused on developing new NMR methods (such as paramagnetic relaxation enhancement, dark state exchange saturation transfer spectroscopy and lifetime line broadening) to detect, characterize and visualize the structure and dynamics of sparsely-populated states of macromolecules, which are important in macromolecular interactions but invisible to conventional structural and biophysical techniques.[28] Examples of include the direct demonstration of rotation-coupled sliding and intermolecular translocation as mechanisms whereby sequence-specific DNA binding proteins locate their target site(s) within an overwhelming sea of non-specific DNA sequences;[29] the detection, visualization and characterization of encounter complexes in protein-protein association;[30] the analysis of the synergistic effects of conformational selection and induced fit in protein-ligand interactions;[31] and the uncovering of "dark", spectroscopically invisible states in interactions of NMR-visible proteins and polypeptides (including intrinsically disordered states) with very large megadalton macromolecular assemblies.[32] The latter includes an atomic-resolution view of the dynamics of the amyloid-β aggregation process.[33] and the demonstration of intrinsic unfoldase/foldase activity of the macromolecular machine GroEL.[34] These various techniques have also been used to uncover the kinetic pathway of pre-nucleation transient oligomerization events and associated structures involving the protein encoded by huntingtin exon-1, which may provide a potential avenue for therapeutic intervention in Huntington's disease, a fatal autosomal dominant, neurodegenerative condition.[35][36]

Scientific impact

Clore is one of the most highly cited scientists in the fields of molecular biophysics, structural biology, biomolecular NMR and chemistry[37][38] with over 540 published scientific articles and an h-index (number of papers cited h or more time) of 142.[39] Clore is also one of only four NIH scientists to have been elected to both the United States National Academy of Sciences and The Royal Society, the other three being Julius Axelrod, Francis Collins and Harold Varmus.

Personal life

Marius Clore was educated at the Lycee Francais Charles de Gaulle in Kensington, London. Marius Clore's father was the film producer Leon Clore whose credits include The French Lieutenant's Woman, and his mother was Miriam Clore (née Werner), a member of the Haganah during the 1948 Arab-Israeli War and subsequently the personal assistant to David Ben Gurion prior to moving to England. Sir Charles Clore and Dame Vivien Duffield are his great uncle and second cousin, respectively. He is married to Carole A. Bewley a senior investigator and Chief of the Laboratory of Bioorganic Chemistry in the National Institute of Diabetes and Digestive and Kidney Diseases at the National Institutes of Health.

Awards and honors

References

  1. ^ a b c Samoray C (2016). "Profile of Marius Clore". Proceedings of the National Academy of Sciences of the United States of America. 113 (45): 12604–12606. doi:10.1073/pnas.1616528113. PMC 5111653. PMID 27799541.{{cite journal}}: CS1 maint: uses authors parameter (link)
  2. ^ a b Clore, G. Marius. "Curriculum Vitae" (PDF). NIDDK. Retrieved 26 June 2020.
  3. ^ "American Institute of Physics Oral History Interviews - Marius Clore interviewed by David Zierler". AIP. 24 June 2020. Retrieved 26 June 2020.
  4. ^ a b c d "G. Marius Clore". Member Directory. National Academy of Sciences. Retrieved 12 March 2015.
  5. ^ a b c "G. Marius Clore". Member Directory. Royal Society. Retrieved 29 April 2020.
  6. ^ "G. Marius Clore, MD, Ph.D., NIH Distinguished Investigator". National Institutes of Health Intramural Research Program. Retrieved 14 August 2018.
  7. ^ "G. Marius Clore, MD, Ph.D., FRS, NIH Distinguished Investigator". National Institute of Diabetes and Digestive and Kidney Diseases. Retrieved 14 August 2018.
  8. ^ "New Members and Foreign Associates of the National Academy of Sciences: G. Marius Clore, Gregory C. Fu, Sir J. Fraser Stoddart, Ei-ichi Negishi". Angewandte Chemie International Edition. 53 (26): 6598. 2014. doi:10.1002/anie.201405510.
  9. ^ Ringe D (1988). "Protein structure: an extra dimension to NMR". Nature. 332 (6162): 303. Bibcode:1988Natur.332..303R. doi:10.1038/332303a0. PMID 3352729. S2CID 32312775.
  10. ^ Dahlquist FW (2006). "Slip sliding away: new insights into DNA-protein recognition". Nature Chemical Biology. 2 (7): 353–354. doi:10.1038/nchembio0706-353. PMID 16783338. S2CID 12357797.
  11. ^ Blundell TL, Fernandez-Recio J (2006). "Cell biology: brief encounters bolster contacts". Nature. 444 (7117): 279–280. Bibcode:2006Natur.444..279B. doi:10.1038/nature05306. PMID 17051147. S2CID 4397989.{{cite journal}}: CS1 maint: uses authors parameter (link)
  12. ^ "Clore named Royal Society Fellow". Retrieved 1 June 2020.
  13. ^ Deshmukh, L., Tugarinov,V., Appella, D.H., Clore, G.M. (2018). "Targeting a dark excited state of HIV-1 nucleocapsid by anti-retroviral thioesters revealed by NMR spectroscopy". Angewandte Chemie International Edition. 57 (10): 2687–2691. doi:10.1002/anie.201713172. PMC 6034507. PMID 29345807.{{cite journal}}: CS1 maint: uses authors parameter (link)
  14. ^ a b "Former Fellows of the Lister Institute of Preventive Medicine". Retrieved 27 June 2020.
  15. ^ Clore, Marius G (2011). "Adventures in Biomolecular NMR" (PDF). In Harris, Robin K; Wasylishen, Roderick L (eds.). Encyclopedia of Magnetic Resonance. John Wiley & Sons. doi:10.1002/9780470034590. hdl:11693/53364. ISBN 9780470034590.
  16. ^ . Archived from the original on 2015-08-18.
  17. ^ "2020 Royal Society press release of outstanding scientists elected as Fellows and Foreign Members".
  18. ^ a b "Book of Members, 1780-2014: Chapter B" (PDF). American Academy of Arts and Sciences.
  19. ^ a b "American Academy of Arts and Sciences Fellows".
  20. ^ a b "Elected Members of Academia Europaea 2015".
  21. ^ Clore GM, Gronenborn AM (1991). "Structures of larger proteins in solution: three- and four-dimensional heteronuclear NMR spectroscopy". Science. 252 (5011): 1390–1399. Bibcode:1991Sci...252.1390M. doi:10.1126/science.2047852. OSTI 83376. PMID 2047852.{{cite journal}}: CS1 maint: uses authors parameter (link)
  22. ^ Clore GM (2000). "Accurate and rapid docking of protein-protein complexes on the basis of intermolecular nuclear Overhauser enhancement data and dipolar couplings by rigid body minimization". Proceedings of the National Academy of Sciences USA. 97 (16): 9021–9025. Bibcode:2000PNAS...97.9021C. doi:10.1073/pnas.97.16.9021. PMC 16814. PMID 10922057.{{cite journal}}: CS1 maint: uses authors parameter (link)
  23. ^ Clore GM, Gronenborn AM (1998). "New methods of structure refinement for macromolecular structure determination by NMR". Proceedings of the National Academy of Sciences of the United States of America. 95 (11): 5891–5898. Bibcode:1998PNAS...95.5891M. doi:10.1073/pnas.95.11.5891. PMC 34492. PMID 9600889.{{cite journal}}: CS1 maint: uses authors parameter (link)
  24. ^ a b Clore GM, Venditti V (2013). "Structure, dynamics and biophysics of the cytoplasmic protein-protein complexes of the bacterial phosphoenolpyruvate:sugar phosphotransferase system". Trends in Biochemical Sciences. 38 (10): 515–530. doi:10.1016/j.tibs.2013.08.003. PMC 3831880. PMID 24055245.{{cite journal}}: CS1 maint: uses authors parameter (link)
  25. ^ Schwieters CD, Clore, GM (2014). "Using small angle solution scattering data in Xplor-NIH structure calculations". Progress in Nuclear Magnetic Resonance Spectroscopy. 80: 1–11. doi:10.1016/j.pnmrs.2014.03.001. PMC 4057650. PMID 24924264.{{cite journal}}: CS1 maint: uses authors parameter (link)
  26. ^ Clore GM, Driscoll PC, Wingfield PT, Gronenborn AM (1990). "Analysis of backbone dynamics of interleukin-1beta using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy". Biochemistry. 29 (32): 7387–7401. doi:10.1021/bi00484a006. PMID 2223770.{{cite journal}}: CS1 maint: uses authors parameter (link)
  27. ^ Schwieters CD, Kuszewski JJ, Tjandra N, Clore GM (2003). "The Xplor-NIH NMR molecular structure determination package". Journal of Magnetic Resonance. 160 (1): 65–73. Bibcode:2003JMagR.160...65S. doi:10.1016/S1090-7807(02)00014-9. PMID 12565051.{{cite journal}}: CS1 maint: uses authors parameter (link)
  28. ^ Anthis NJ, Clore GM (2015). "Visualizing transient dark states by NMR spectroscopy". Quarterly Reviews of Biophysics. 48 (1): 35–116. doi:10.1017/S0033583514000122. PMC 6276111. PMID 25710841.{{cite journal}}: CS1 maint: uses authors parameter (link)
  29. ^ Iwahara J, Clore GM (2006). "Detecting transient intermediates in macromolecular binding by paramagnetic NMR". Nature. 440 (7088): 1227–1230. Bibcode:2006Natur.440.1227I. doi:10.1038/nature04673. PMID 16642002. S2CID 4427016.{{cite journal}}: CS1 maint: uses authors parameter (link)
  30. ^ Tang C, Iwahara J, Clore GM (2006). "Visualization of transient encounter complexes in protein-protein association". Nature. 444 (7117): 383–386. Bibcode:2006Natur.444..383T. doi:10.1038/nature05201. PMID 17051159. S2CID 4422087.{{cite journal}}: CS1 maint: uses authors parameter (link)
  31. ^ Tang C, Schwieters CD, Clore GM (2007). "Open-to-closed transition in apo-maltose-binding protein visualized by paramagnetic NMR". Nature. 449 (7165): 1078–1082. Bibcode:2007Natur.449.1078T. doi:10.1038/nature06232. PMID 17960247. S2CID 4362128.{{cite journal}}: CS1 maint: uses authors parameter (link)
  32. ^ "NMR advance brings proteins into the open". Neurosciencenews.com. 25 June 2013.
  33. ^ Fawzi NL, Ying J, Ghirlando R, Torchia DA, Clore GM (2011). "Atomic resolution dynamics on the surface of amyloid beta protofibrils probed by solution NMR". Nature. 480 (7376): 268–272. Bibcode:2011Natur.480..268F. doi:10.1038/nature10577. PMC 3237923. PMID 22037310.{{cite journal}}: CS1 maint: uses authors parameter (link)
  34. ^ Libich, D.S., Tugarinov, V., Clore, G.M. (2015). "Intrinsic unfoldase/foldase activity of the chaperonin GroEL directly demonstrated using multinuclear relaxation-based NMR". Proc. Natl. Acad. Sci. U.S.A. 112 (29): 8817–8823. Bibcode:2015PNAS..112.8817L. doi:10.1073/pnas.1510083112. PMC 4517251. PMID 26124125.{{cite journal}}: CS1 maint: uses authors parameter (link)
  35. ^ Kotler, S.A., Tugarinov, V., Schmidt, T., Ceccon, A., Libich, D.S., Ghirlando, R., Schwieters, C.D., Clore, G.M. (2019). "probing the initial transient oligomerization events facilitating Huntingtin fibril nucleation at atomic resolution by relaxation-based NMR". Proc. Natl. Acad. Sci. U.S.A. 116 (9): 3562–3571. Bibcode:2019PNAS..116.3562K. doi:10.1073/pnas.1821216116. PMC 6397591. PMID 30808748.{{cite journal}}: CS1 maint: uses authors parameter (link)
  36. ^ Ceccon, A., Tugarinov, V., Ghirlando, R., Clore, G.M. (2020). "Abrogation of prenucleation, transient oligomerization of the huntingtin exon-1 protein by human profilin". Proc. Natl. Acad. Sci. U.S.A. 117 (11): 5844–5852. Bibcode:2020PNAS..117.5844C. doi:10.1073/pnas.1922264117. PMC 7084121. PMID 32127471.{{cite journal}}: CS1 maint: uses authors parameter (link)
  37. ^ "Top 10 researchers in chemistry based on total citations". Times Higher Education. 9 October 2008.
  38. ^ "Royal Society of Chemistry h-index ranking of living chemists" (PDF).
  39. ^ "Google scholar profile".
  40. ^ "G. Marius Clore 2021 Murray Goodman Memorial Prize Winner".
  41. ^ "UCL Awards 2021 Honorary Degrees and Fellowships". 15 July 2021.
  42. ^ "G. Marius Clore 2021 Royal Society of Chemistry Khorana Prize Winner".
  43. ^ "G. Marius Clore to Receive Biophysical Society 2020 Innovation Award".
  44. ^ "Biophysical Society September 2019 Press Release".
  45. ^ "Biochemical Society Award Winners for 2013 - Biochemist e-volution" (PDF). Biochemical Society.
  46. ^ "The Centenary Award". biochemistry.org.
  47. ^ "Centenary Prize Winner 2011". rsc.org.
  48. ^ . Archived from the original on 2015-10-27.
  49. ^ Chemical Society of Washington Hillebrand Award
  50. ^ "American Society of Biochemistry and Molecular Biology Today, May 2011, ASBMB member update p. 6" (PDF).
  51. ^ "Fellow of the Biophysical Society Award". biophysics.org.
  52. ^ . lister-institute.org.uk. Archived from the original on 2015-08-05. Retrieved 2015-02-19.
  53. ^ "Fellows of the American Associastion for the Advancement of Science".
  54. ^ . Archived from the original on 2015-02-14. Retrieved 2015-02-19.
  55. ^ "NIDDK scientists share award" (PDF). The NIH Record (1993) volume 45(17), page 12.

External links

  • G. Marius Clore laboratory homepage
  • Oral history interview transcript with Marius Clore on 23 March 2020, American Institute of Physics, Niels Bohr Library & Archives
  • Listing on the United States National Academy of Sciences web site
  • Listing on the Royal Society web site
  • Listing on the American Academy of Arts and Sciences web site
  • Listing on the Academia Europaea web site
  • Listing on NIDDK/NIH web site
  • Listing on NIH Intramural Research Program web site
  • G. Marius Clore publications indexed by Google Scholar
  • G. Marius Clore Orcid ID
  • Marius Clore on Landmark Article in the Journal of Magnetic Resonance
  • Marius Clore Lecture on "Transient Prenucleation Oligomerization of Huntingtin" at the ICMRBS Webinar on Emerging Topics in Biomolecular Resonance (4/15/2021)
  • List of University College London Honorary Graduates

March 05, 2023
marius, clore, frsc, british, born, anglo, american, molecular, biophysicist, structural, biologist, born, london, dual, citizen, member, national, academy, sciences, fellow, royal, society, distinguished, investigator, chief, molecular, structural, biophysics. G Marius Clore MAE FRSC FRS is a British born Anglo American molecular biophysicist and structural biologist He was born in London U K and is a dual U S U K Citizen 1 2 3 He is a Member of the National Academy of Sciences 4 a Fellow of the Royal Society 5 a NIH Distinguished Investigator and the Chief of the Molecular and Structural Biophysics Section in the Laboratory of Chemical Physics of the National Institute of Diabetes and Digestive and Kidney Diseases at the U S National Institutes of Health 6 7 He is known for his foundational work in three dimensional protein and nucleic acid structure determination by biomolecular NMR spectroscopy 8 for advancing experimental approaches to the study of large macromolecules and their complexes by NMR 9 and for developing NMR based methods to study rare conformational states in protein nucleic acid 10 and protein protein 11 recognition 12 Clore s discovery of previously undetectable functionally significant rare transient states of macromolecules has yielded fundamental new insights into the mechanisms of important biological processes and in particular the significance of weak interactions and the mechanisms whereby the opposing constraints of speed and specificity are optimized Further Clore s work opens up a new era of pharmacology and drug design as it is now possible to target structures and conformations that have been heretofore unseen 13 G Marius CloreMAE FRSC FRSBornGideon Marius Clore1955 1 London U K NationalityBritishCitizenshipBritish AmericanAlma materUniversity College London and University College Hospital Medical School London U K Known forLaying the foundations for three dimensional protein structure determination in solution by NMR developing innovative approaches for extending NMR to larger and more complex systems and using NMR to uncover invisible states of proteinsAwards Member of the National Academy of Sciences Fellow of the Royal Society Fellow of the American Academy of Arts and Sciences Foreign Member of the Academia Europaea Royal Society of Chemistry Centenary Prize 2011 Biochemical Society Centenary Award 2013 Royal Society of Chemistry Khorana Prize 2021 Scientific careerFieldsMolecular Biophysics Nuclear Magnetic Resonance Structural Biology ChemistryInstitutionsMRC National Institute for Medical Research London Max Planck Institute of Biochemistry Martinsried Germany National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda Maryland U S Doctoral advisorSir Arnold Burgen FRSNotable studentsRobert T Clubb Hartmut Oschkinat Julie Forman Kay FRSInfluencesSir Arnold Burgen FRSMax Perutz FRSRobert Huber ForMemRSAttila SzaboWilliam EatonAd BaxInfluencedAd BaxLewis E Kay FRSAxel BrungerWebsitegmclore wbr org Contents 1 Biography 2 Research 2 1 3D structure determination in solution by NMR 2 2 Detection and visualization of excited and sparsely populated states 2 3 Scientific impact 3 Personal life 4 Awards and honors 5 References 6 External linksBiography EditClore received his undergraduate degree with first class honours in biochemistry from University College London in 1976 and medical degree from UCL Medical School in 1979 4 After completing house physician and house surgeon appointments at University College Hospital and St Charles Hospital part of the St Mary s Hospital group respectively he was a member of the scientific staff of the Medical Research Council National Institute for Medical Research from 1980 to 1984 He received his PhD from the National Institute for Medical Research in Physical Biochemistry in 1982 He was awarded a joint Lister Institute Research Fellowship from the Lister Institute of Preventive Medicine which he held from 1982 to 1984 at the Medical Research Council 14 In 1984 he joined the Max Planck Institute for Biochemistry in Martinsried Germany where he headed the Biological NMR department from 1984 to 1988 1 2 In 1988 Clore was recruited to the National Institutes of Health NIH Laboratory of Chemical Physics National Institute of Diabetes and Digestive and Kidney Diseases located in Bethesda Maryland U S where he interacted closely in the late 1980s and early 1990s with NIH colleagues Ad Bax Angela Gronenborn and Dennis Torchia on the development of multidimensional heteronuclear NMR spectroscopy and a structural biology effort aimed at proteins involved in the pathogenesis of HIV AIDS 15 He has remained at the NIH ever since and is currently a NIH Distinguished Investigator and Chief of the Section on Molecular and Structural Biophysics at the NIH 4 He is an elected Member of the United States National Academy of Sciences 16 a Fellow of the Royal Society 17 a Fellow of the American Academy of Arts and Sciences 18 19 and a Foreign Member of the Academia Europaea Biochemistry and Molecular Biology Section 20 Clore s citation upon election to the Royal Society reads Clore pioneered the development of NMR for determining three dimensional structures of biological macromolecules and has consistently extended the frontiers of NMR to ever more complex systems His work on the development of paramagnetic and other relaxation based NMR experiments to detect and visualize transient rare states of macromolecules invisible to conventional structural and biophysical techniques has shed unique insights into how macromolecules efficiently locate their binding partners provided the first atomic view of the dynamic amyloid Ass assembly process from disordered peptides into protofibrils and directly demonstrated that the apo state of the chaperonin GroEL possesses intrinsic foldase unfoldase activities 5 Research Edit3D structure determination in solution by NMR Edit Clore played a pivotal role in the development of three and four dimensional NMR spectroscopy 21 the use of residual dipolar couplings for structure determination 22 the development of simulated annealing and restrained molecular dynamics for three dimensional protein and nucleic acid structure determination 23 the solution NMR structure determination of large protein complexes 24 the development of the combined use of NMR and small angle X ray scattering in solution structure determination 25 and the analysis and characterization of protein dynamics by NMR 26 Clore s work on complexes of all the cytoplasmic components of the bacterial phosphotransferase system PTS led to significant insights into how signal transduction proteins recognize multiple structurally dissimilar partners by generating similar binding surfaces from completely different structural elements and exploiting side chain conformational plasticity 24 Clore is also one of the main authors of the very widely used XPLOR NIH NMR structure determination program 27 Detection and visualization of excited and sparsely populated states Edit Clore s recent work has focused on developing new NMR methods such as paramagnetic relaxation enhancement dark state exchange saturation transfer spectroscopy and lifetime line broadening to detect characterize and visualize the structure and dynamics of sparsely populated states of macromolecules which are important in macromolecular interactions but invisible to conventional structural and biophysical techniques 28 Examples of include the direct demonstration of rotation coupled sliding and intermolecular translocation as mechanisms whereby sequence specific DNA binding proteins locate their target site s within an overwhelming sea of non specific DNA sequences 29 the detection visualization and characterization of encounter complexes in protein protein association 30 the analysis of the synergistic effects of conformational selection and induced fit in protein ligand interactions 31 and the uncovering of dark spectroscopically invisible states in interactions of NMR visible proteins and polypeptides including intrinsically disordered states with very large megadalton macromolecular assemblies 32 The latter includes an atomic resolution view of the dynamics of the amyloid b aggregation process 33 and the demonstration of intrinsic unfoldase foldase activity of the macromolecular machine GroEL 34 These various techniques have also been used to uncover the kinetic pathway of pre nucleation transient oligomerization events and associated structures involving the protein encoded by huntingtin exon 1 which may provide a potential avenue for therapeutic intervention in Huntington s disease a fatal autosomal dominant neurodegenerative condition 35 36 Scientific impact Edit Clore is one of the most highly cited scientists in the fields of molecular biophysics structural biology biomolecular NMR and chemistry 37 38 with over 540 published scientific articles and an h index number of papers cited h or more time of 142 39 Clore is also one of only four NIH scientists to have been elected to both the United States National Academy of Sciences and The Royal Society the other three being Julius Axelrod Francis Collins and Harold Varmus Personal life EditMarius Clore was educated at the Lycee Francais Charles de Gaulle in Kensington London Marius Clore s father was the film producer Leon Clore whose credits include The French Lieutenant s Woman and his mother was Miriam Clore nee Werner a member of the Haganah during the 1948 Arab Israeli War and subsequently the personal assistant to David Ben Gurion prior to moving to England Sir Charles Clore and Dame Vivien Duffield are his great uncle and second cousin respectively He is married to Carole A Bewley a senior investigator and Chief of the Laboratory of Bioorganic Chemistry in the National Institute of Diabetes and Digestive and Kidney Diseases at the National Institutes of Health Awards and honors Edit2021 Murray Goodman Memorial Prize 40 2021 Honorary Doctorate of Science DSc from University College London 41 2021 Royal Society of Chemistry Khorana Prize 42 2020 Elected Fellow of the Royal Society 5 2020 Biophysical Society Innovation Award 43 44 2015 Elected Foreign Member of the Academia Europaea 20 2014 Elected Member of the United States National Academy of Sciences Biophysics and Computational Biology section 4 2012 Biochemical Society 2013 Centenary Award previously known as the Jubilee Medal and Sir Frederick Gowland Hopkins Memorial Lecture U K 45 46 2011 Royal Society of Chemistry Centenary Prize 47 2011 Elected Fellow of the International Society of Magnetic Resonance 48 2010 Elected Fellow of the American Academy of Arts and Sciences 18 19 2010 Hillebrand Award of the Washington Chemical Society 49 50 2009 Elected Fellow of the Biophysical Society 51 2003 Elected Member of the Lister Institute of Preventive Medicine U K 14 52 2001 Original member Institute for Scientific Information ISI Highly Cited Researchers Database in Biology amp Biochemistry and Chemistry sections 1999 Elected Fellow of the American Association for the Advancement of Science 53 1993 Dupont Merck Young Investigator Award of the Protein Society 54 55 1990 Elected Fellow of the Royal Society of Chemistry FRSC U K References Edit a b c Samoray C 2016 Profile of Marius Clore Proceedings of the National Academy of Sciences of the United States of America 113 45 12604 12606 doi 10 1073 pnas 1616528113 PMC 5111653 PMID 27799541 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link a b Clore G Marius Curriculum Vitae PDF NIDDK Retrieved 26 June 2020 American Institute of Physics Oral History Interviews Marius Clore interviewed by David Zierler AIP 24 June 2020 Retrieved 26 June 2020 a b c d G Marius Clore Member Directory National Academy of Sciences Retrieved 12 March 2015 a b c G Marius Clore Member Directory Royal Society Retrieved 29 April 2020 G Marius Clore MD Ph D NIH Distinguished Investigator National Institutes of Health Intramural Research Program Retrieved 14 August 2018 G Marius Clore MD Ph D FRS NIH Distinguished Investigator National Institute of Diabetes and Digestive and Kidney Diseases Retrieved 14 August 2018 New Members and Foreign Associates of the National Academy of Sciences G Marius Clore Gregory C Fu Sir J Fraser Stoddart Ei ichi Negishi Angewandte Chemie International Edition 53 26 6598 2014 doi 10 1002 anie 201405510 Ringe D 1988 Protein structure an extra dimension to NMR Nature 332 6162 303 Bibcode 1988Natur 332 303R doi 10 1038 332303a0 PMID 3352729 S2CID 32312775 Dahlquist FW 2006 Slip sliding away new insights into DNA protein recognition Nature Chemical Biology 2 7 353 354 doi 10 1038 nchembio0706 353 PMID 16783338 S2CID 12357797 Blundell TL Fernandez Recio J 2006 Cell biology brief encounters bolster contacts Nature 444 7117 279 280 Bibcode 2006Natur 444 279B doi 10 1038 nature05306 PMID 17051147 S2CID 4397989 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Clore named Royal Society Fellow Retrieved 1 June 2020 Deshmukh L Tugarinov V Appella D H Clore G M 2018 Targeting a dark excited state of HIV 1 nucleocapsid by anti retroviral thioesters revealed by NMR spectroscopy Angewandte Chemie International Edition 57 10 2687 2691 doi 10 1002 anie 201713172 PMC 6034507 PMID 29345807 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link a b Former Fellows of the Lister Institute of Preventive Medicine Retrieved 27 June 2020 Clore Marius G 2011 Adventures in Biomolecular NMR PDF In Harris Robin K Wasylishen Roderick L eds Encyclopedia of Magnetic Resonance John Wiley amp Sons doi 10 1002 9780470034590 hdl 11693 53364 ISBN 9780470034590 2014 Press release of National Academy of Sciences Members and Foreign Associates Elected Archived from the original on 2015 08 18 2020 Royal Society press release of outstanding scientists elected as Fellows and Foreign Members a b Book of Members 1780 2014 Chapter B PDF American Academy of Arts and Sciences a b American Academy of Arts and Sciences Fellows a b Elected Members of Academia Europaea 2015 Clore GM Gronenborn AM 1991 Structures of larger proteins in solution three and four dimensional heteronuclear NMR spectroscopy Science 252 5011 1390 1399 Bibcode 1991Sci 252 1390M doi 10 1126 science 2047852 OSTI 83376 PMID 2047852 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Clore GM 2000 Accurate and rapid docking of protein protein complexes on the basis of intermolecular nuclear Overhauser enhancement data and dipolar couplings by rigid body minimization Proceedings of the National Academy of Sciences USA 97 16 9021 9025 Bibcode 2000PNAS 97 9021C doi 10 1073 pnas 97 16 9021 PMC 16814 PMID 10922057 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Clore GM Gronenborn AM 1998 New methods of structure refinement for macromolecular structure determination by NMR Proceedings of the National Academy of Sciences of the United States of America 95 11 5891 5898 Bibcode 1998PNAS 95 5891M doi 10 1073 pnas 95 11 5891 PMC 34492 PMID 9600889 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link a b Clore GM Venditti V 2013 Structure dynamics and biophysics of the cytoplasmic protein protein complexes of the bacterial phosphoenolpyruvate sugar phosphotransferase system Trends in Biochemical Sciences 38 10 515 530 doi 10 1016 j tibs 2013 08 003 PMC 3831880 PMID 24055245 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Schwieters CD Clore GM 2014 Using small angle solution scattering data in Xplor NIH structure calculations Progress in Nuclear Magnetic Resonance Spectroscopy 80 1 11 doi 10 1016 j pnmrs 2014 03 001 PMC 4057650 PMID 24924264 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Clore GM Driscoll PC Wingfield PT Gronenborn AM 1990 Analysis of backbone dynamics of interleukin 1beta using two dimensional inverse detected heteronuclear 15N 1H NMR spectroscopy Biochemistry 29 32 7387 7401 doi 10 1021 bi00484a006 PMID 2223770 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Schwieters CD Kuszewski JJ Tjandra N Clore GM 2003 The Xplor NIH NMR molecular structure determination package Journal of Magnetic Resonance 160 1 65 73 Bibcode 2003JMagR 160 65S doi 10 1016 S1090 7807 02 00014 9 PMID 12565051 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Anthis NJ Clore GM 2015 Visualizing transient dark states by NMR spectroscopy Quarterly Reviews of Biophysics 48 1 35 116 doi 10 1017 S0033583514000122 PMC 6276111 PMID 25710841 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Iwahara J Clore GM 2006 Detecting transient intermediates in macromolecular binding by paramagnetic NMR Nature 440 7088 1227 1230 Bibcode 2006Natur 440 1227I doi 10 1038 nature04673 PMID 16642002 S2CID 4427016 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Tang C Iwahara J Clore GM 2006 Visualization of transient encounter complexes in protein protein association Nature 444 7117 383 386 Bibcode 2006Natur 444 383T doi 10 1038 nature05201 PMID 17051159 S2CID 4422087 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Tang C Schwieters CD Clore GM 2007 Open to closed transition in apo maltose binding protein visualized by paramagnetic NMR Nature 449 7165 1078 1082 Bibcode 2007Natur 449 1078T doi 10 1038 nature06232 PMID 17960247 S2CID 4362128 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link NMR advance brings proteins into the open Neurosciencenews com 25 June 2013 Fawzi NL Ying J Ghirlando R Torchia DA Clore GM 2011 Atomic resolution dynamics on the surface of amyloid beta protofibrils probed by solution NMR Nature 480 7376 268 272 Bibcode 2011Natur 480 268F doi 10 1038 nature10577 PMC 3237923 PMID 22037310 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Libich D S Tugarinov V Clore G M 2015 Intrinsic unfoldase foldase activity of the chaperonin GroEL directly demonstrated using multinuclear relaxation based NMR Proc Natl Acad Sci U S A 112 29 8817 8823 Bibcode 2015PNAS 112 8817L doi 10 1073 pnas 1510083112 PMC 4517251 PMID 26124125 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Kotler S A Tugarinov V Schmidt T Ceccon A Libich D S Ghirlando R Schwieters C D Clore G M 2019 probing the initial transient oligomerization events facilitating Huntingtin fibril nucleation at atomic resolution by relaxation based NMR Proc Natl Acad Sci U S A 116 9 3562 3571 Bibcode 2019PNAS 116 3562K doi 10 1073 pnas 1821216116 PMC 6397591 PMID 30808748 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Ceccon A Tugarinov V Ghirlando R Clore G M 2020 Abrogation of prenucleation transient oligomerization of the huntingtin exon 1 protein by human profilin Proc Natl Acad Sci U S A 117 11 5844 5852 Bibcode 2020PNAS 117 5844C doi 10 1073 pnas 1922264117 PMC 7084121 PMID 32127471 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint uses authors parameter link Top 10 researchers in chemistry based on total citations Times Higher Education 9 October 2008 Royal Society of Chemistry h index ranking of living chemists PDF Google scholar profile G Marius Clore 2021 Murray Goodman Memorial Prize Winner UCL Awards 2021 Honorary Degrees and Fellowships 15 July 2021 G Marius Clore 2021 Royal Society of Chemistry Khorana Prize Winner G Marius Clore to Receive Biophysical Society 2020 Innovation Award Biophysical Society September 2019 Press Release Biochemical Society Award Winners for 2013 Biochemist e volution PDF Biochemical Society The Centenary Award biochemistry org Centenary Prize Winner 2011 rsc org List of elected ISMAR fellows Archived from the original on 2015 10 27 Chemical Society of Washington Hillebrand Award American Society of Biochemistry and Molecular Biology Today May 2011 ASBMB member update p 6 PDF Fellow of the Biophysical Society Award biophysics org Members Former Fellows lister institute org uk Archived from the original on 2015 08 05 Retrieved 2015 02 19 Fellows of the American Associastion for the Advancement of Science Protein Society Young Investigator Award Archived from the original on 2015 02 14 Retrieved 2015 02 19 NIDDK scientists share award PDF The NIH Record 1993 volume 45 17 page 12 External links EditG Marius Clore laboratory homepage Oral history interview transcript with Marius Clore on 23 March 2020 American Institute of Physics Niels Bohr Library amp Archives Listing on the United States National Academy of Sciences web site Listing on the Royal Society web site Listing on the American Academy of Arts and Sciences web site Listing on the Academia Europaea web site Listing on NIDDK NIH web site Listing on NIH Intramural Research Program web site G Marius Clore publications indexed by Google Scholar G Marius Clore Orcid ID Marius Clore on Landmark Article in the Journal of Magnetic Resonance Marius Clore Lecture on Transient Prenucleation Oligomerization of Huntingtin at the ICMRBS Webinar on Emerging Topics in Biomolecular Resonance 4 15 2021 List of University College London Honorary Graduates Retrieved from https en wikipedia org w index php title G Marius Clore amp oldid 1141514591, wikipedia, wiki, book, books, library,

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