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Wikipedia

Cellular agriculture

January 03, 2023

Cellular agriculture focuses on the production of agricultural products from cell cultures using a combination of biotechnology, tissue engineering, molecular biology, and synthetic biology to create and design new methods of producing proteins, fats, and tissues that would otherwise come from traditional agriculture.[1] Most of the industry is focused on animal products such as meat, milk, and eggs, produced in cell culture rather than raising and slaughtering farmed livestock which is associated with substantial global problems of detrimental environmental impacts (e.g. of meat production), animal welfare, food security and human health[2][3][4].[5] Cellular agriculture is field of the biobased economy. The most well known cellular agriculture concept is cultured meat.

A video by New Harvest / Xprize explaining the development of cultured meat and a "post-animal bio-economy, driven by lab grown protein (meat, eggs, milk)". (Runtime 3:09)

History

For a chronological guide, see Timeline of cellular agriculture.

Although cellular agriculture is a nascent scientific discipline, cellular agriculture products were first commercialized in the early 20th century with insulin and rennet.[6]

On March 24, 1990, the FDA approved a bacterium that had been genetically engineered to produce rennet, making it the first genetically engineered product for food.[7] Rennet is a mixture of enzymes that turns milk into curds and whey in cheese making. Traditionally, rennet is extracted from the inner lining of the fourth stomach of calves. Today, cheese making processes use rennet enzymes from genetically engineered bacteria, fungi, or yeasts because they are unadulterated, more consistent, and less expensive than animal-derived rennet.[8]

In 2004, Jason Matheny founded New Harvest, whose mission is to "accelerate breakthroughs in cellular agriculture".[9] New Harvest is the only organization focused exclusively on advancing the field of cellular agriculture and provided the first PhD funding specifically for cellular agriculture, at Tufts University.[10]

By 2014, IndieBio, a synthetic biology accelerator in San Francisco, has incubated several cellular agriculture startups, hosting Muufri (making milk from cell culture, now Perfect Day Foods), The EVERY Company (making egg whites from cell culture), Gelzen (making gelatin from bacteria and yeast, now Geltor), Afineur (making cultured coffee beans) and Pembient (making rhino horn). Muufri and The EVERY Company were both initially sponsored by New Harvest.

In 2015, Mercy for Animals created The Good Food Institute, which promotes plant-based and cellular agriculture.[11]

Also in 2015, Isha Datar coined the term "cellular agriculture" (often shortened to "cell ag") in a New Harvest Facebook group.[12][13]

On July 13, 2016, New Harvest hosted the world's first international conference on cellular agriculture in San Francisco, California.[9] The day after the conference, New Harvest hosted the first closed-door workshop for industry, academic, and government stakeholders in cellular agriculture.[14]

Research tools

Several key research tools are at the foundation of research in cellular agriculture. These include:[15]

Cell lines

A fundamental missing piece in the advancement of cultured meat is the availability of the appropriate cellular materials. While some methods and protocols from human and mouse cell culture may apply to agricultural cellular materials, it has become clear that most do not. This is evidenced by the fact that established protocols for creating human and mouse embryonic stem cells have not succeeded in establishing ungulate embryonic stem cell lines.[16][17][18]

The ideal criteria for cell lines for the purpose of cultured meat production include immortality, high proliferative ability, surface independence, serum independence, and tissue-forming ability. The specific cell types most suitable for cellular agriculture are likely to differ from species to species.[19][20]

Growth media

Conventional methods for growing animal tissue in culture involves the use of fetal bovine serum (FBS). FBS is a blood product extracted from fetal calves. This product supplies cells with nutrients and stimulating growth factors, but is unsustainable and resource-heavy to produce, with large batch-to-batch variation.[21] Cultured meat companies have been putting significant resources into alternative growth media.

After the creation of the cell lines, efforts to remove serum from the growth media are key to the advancement of cellular agriculture as fetal bovine serum has been the target of most criticisms of cellular agriculture and cultured meat production. It is likely that two different media formulations will be required for each cell type: a proliferation media, for growth, and a differentiation media, for maturation.[22]

Scaling technologies

As biotechnological processes are scaled, experiments start to become increasingly expensive, as bioreactors of increasing volume will have to be created. Each increase in size will require a re-optimization of various parameters such as unit operations, fluid dynamics, mass transfer, and reaction kinetics.

Scaffold materials

For cells to form tissue, it is helpful for a material scaffold to be added to provide structure. Scaffolds are crucial for cells to form tissues larger than 100 µm across. An ideal scaffold must be non-toxic for the cells, edible, and allow for the flow of nutrients and oxygen. It must also be cheap and easy to produce on a large scale without the need for animals.

3D tissue systems

The final phase for creating cultured meat involves bringing together all the previous pieces of research to create large (>100 µm in diameter) pieces of tissue that can be made of mass-produced cells without the need for serum, where the scaffold is suitable for cells and humans.

Applications

While the majority of discussion has been around food applications, particular cultured meat, cellular agriculture can be used to create any kind of agricultural product, including those that never involved animals to begin with, like Ginkgo Biowork's fragrances.

Meat

Main article: Cultured meat

Cultured meat (also known by other names) is a meat produced by in vitro cell cultures of animal cells.[23] It is a form of cellular agriculture, with such agricultural methods being explored in the context of increased consumer demand for protein.[24]

Cultured meat is produced using tissue engineering techniques traditionally used in regenerative medicines.[25] The concept of cultured meat was introduced to wider audiences by Jason Matheny in the early 2000s after he co-authored a paper[26] on cultured meat production and created New Harvest, the world's first nonprofit organization dedicated to in-vitro meat research.[27]

Cultured meat may have the potential to address substantial global problems of the environmental impact of meat production, animal welfare, food security and human health.[2][3][4][28][29] Specifically, it can be thought of in the context of the mitigation of climate change.[24]

Lecture The Meat Revolution at the World Economic Forum by Mark Post of the University of Maastricht about in vitro meat (runtime 20:16)
A video by New Harvest / Xprize explaining the development of cultured meat and a "post-animal bio-economy, driven by lab grown protein (meat, eggs, milk)" (runtime 3:09)

In 2013, professor Mark Post at Maastricht University pioneered a proof-of-concept for cultured meat by creating the first hamburger patty grown directly from cells. Since then, other cultured meat prototypes have gained media attention: SuperMeat opened a farm-to-fork restaurant called "The Chicken"[30] in Tel Aviv to test consumer reaction to its "Chicken" burger,[31] while the "world's first commercial sale of cell-cultured meat" occurred in December 2020 at the Singapore restaurant "1880", where cultured meat manufactured by the US firm Eat Just was sold.[32]

While most efforts in the space focus on common meats such as pork, beef, and chicken which comprise the bulk of consumption in developed countries,[33] some new companies such as Orbillion Bio have focused on high end or unusual meats including Elk, Lamb, Bison, and the prized Wagyu strain of beef.[34] Avant Meats has brought cultured grouper fish to market [35] as other companies have started to pursue cultivating additional fish species and other seafood.[36]

The production process is constantly evolving, driven by multiple companies and research institutions.[37] The applications of cultured meat have led to ethical, health, environmental, cultural, and economic discussions.[38] In terms of market strength, data published by the non-governmental organization Good Food Institute found that in 2021 cultivated meat companies attracted $140 million in Europe alone.[24] Currently cultured meat is served at special events and few high end restaurants, mass production of cultured meat has not started yet.

 
In 2021, researchers presented a bioprinting method to produce steak-like cultured meat.[39][40]

In 2020, the world's first regulatory approval for a cultivated meat product was awarded by the Government of Singapore. The chicken meat was grown in a bioreactor in a fluid of amino acids, sugar, and salt.[41] The chicken nuggets food products are ~70% lab-grown meat, while the remainder is made from mung bean proteins and other ingredients. The company pledged to strive for price parity with premium "restaurant" chicken servings.[42][43]

For an overview of start-up companies and non-profit organisations working on cellular meat, see Cultured meat § Companies.

Dairy

  • Perfect Day is a San Francisco-based startup that started as the New Harvest Dairy Project and was incubated by IndieBio in 2014. Perfect Day is making dairy from yeast instead of cows.[44][45] The company changed its name from Muufri to Perfect Day in August 2016.[46]
  • Formo, based in Germany, is a startup making dairy products using microbial precision fermentation.[56]
  • Imagindairy is attempting to create dairy from bioengineered yeast.[57][58]

Eggs

  • The EVERY Company is a San Francisco-based startup that started as the New Harvest Egg Project and was incubated by IndieBio in 2015. The EVERY Company is making egg whites from yeast instead of eggs.[59]

Gelatin

  • Geltor is a San Francisco-based startup that was incubated by IndieBio in 2015. Geltor is developing a proprietary protein production platform that uses bacteria and yeast to produce gelatin.[60][61]

Coffee

In 2021, media outlets reported that the world's first synthetic coffee products have been created by two biotechnology companies, still awaiting regulatory approvals for near-term commercialization.[62][63][64] Such products – which can be produced via cellular agriculture in bioreactors[64] and for which multiple companies' R&D have acquired substantial funding – may have equal or highly similar effects, composition and taste as natural products but use less water, generate less carbon emissions, require less labor[63][additional citation(s) needed] and cause no deforestation.[62] Products that equal naturally grown coffee on the chemical molecular level technically would not be "coffee substitutes" but differ only in their method of production – and hence be "lab-grown coffee".[63]

Organizations working on cellular coffee include:

  • Afineur is a Brooklyn-based startup using biotechnology and smart fermentations to improve the nutritional profile and taste of plant-based food, starting with craft coffee.[65]

Horseshoe crab blood

  • Sothic Bioscience is a Cork-based startup incubated by IndieBio in 2015. Sothic Bioscience is building a platform for biosynthetic horseshoe crab blood production. Horseshoe crab blood contains limulus amebocyte lysate (LAL), which is the gold standard in validating medical equipment and medication.[66][67]

Fish

Cellular agriculture could be used for commercial fish feed.

  • Finless Foods is working to develop and mass manufacture marine animal food products.[68]
  • Wild Type is a San Francisco-based startup focused on creating cultured meat to address items such as climate change, food security, and health.[69][70]

Fragrances

  • Ginkgo Bioworks is a Boston-based organism design company culturing fragrances and designing custom microbes.[71]

Silk

See also: § Similar fields of research and production
  • Spiber is a Japan-based company decoding the gene responsible for the production of fibroin in spiders and then bioengineering bacteria with recombinant DNA to produce the protein, which they then spin into their artificial silk.[72][73]
  • Bolt Threads is a California-based company creating engineered silk fibers based on proteins found in spider silk that can be produced at commercial scale. Bolt examines the DNA of spiders and then replicates those genetic sequences in other ingredients to create a similar silk fiber. Bolt's silk is made primarily of sugar, water, salts, and yeast. Through a process called wet spinning, this liquid is spun into fiber, similar to the way fibers like acrylic and rayon are made.[74][75][76]

Leather

  • Modern Meadow is a Brooklyn-based startup growing collagen, a protein found in animal skin, to make biofabricated leather.[77]

Pet food

  • Clean Meat cluster lists Because Animals,[78] Wild Earth and Bond Pet Foods[79] as participants in developing pet foods that use cultured meat.[citation needed]

Wood

In 2022, scientists reported the first 3D-printed lab-grown wood. It is unclear if it could ever be used on a commercial scale (e.g. with sufficient production efficiency and quality).[80][81]

Issues

See also: Synthetic biology § Ethics

Degrowth, green growth and circular economy

Further information: Degrowth

The bioeconomy has largely been associated with visions of "green growth".[82] A study found that a "circular bioeconomy" may be "necessary to build a carbon neutral future in line with the climate objectives of the Paris Agreement".[83] However, some are concerned that with a focus or reliance on technological progress a fundamentally unsustainable socioeconomic model might be maintained rather than be changed.[84] Some are concerned it that may not lead to a ecologization of the economy but to an economization of the biological, "the living" and caution that potentials of non-bio-based techniques to achieve greater sustainability need to be considered.[84] A study found that the, as of 2019, current EU interpretation of the bioeconomy is "diametrically opposite to the original narrative of Baranoff and Georgescu-Roegen that told us that expanding the share of activities based on renewable resources in the economy would slow down economic growth and set strict limits on the overall expansion of the economy".[85] Furthermore, some caution that "Silicon Valley and food corporations" could use bioeconomy technologies for greenwashing and monopoly-concentrations.[86] The bioeconomy, its potentials, disruptive new modes of production and innovations may distract from the need for systemic structural socioeconomic changes[87][88] and provide a false illusion of technocapitalist utopianism/optimism that suggests technological fixes[89] may make it possible to sustain contemporary patterns and structures, pre-empting structural changes.

Unemployment and work reallocation

Further information: Technological unemployment

Many farmers depend on conventional methods of producing crops and many of them live in developing economies.[90] Cellular agriculture for products such as synthetic coffee could, if the contemporary socioeconomic context (the socioeconomic system's mechanisms such as incentives and resource distribution mechanisms like markets) remains unaltered (e.g. in nature, purposes, scopes, limits and degrees), threaten their employment and livelihoods as well as the respective nation's economy and social stability. A study concluded that "given the expertise required and the high investment costs of the innovation, it seems unlikely that cultured meat immediately benefits the poor in developing countries" and emphasized that animal agriculture is often essential for the subsistence for farmers in poor countries.[91] However, not only developing countries may be affected.[92]

Patents, intellectual property and monopolies

Observers worry that the bioeconomy will become as opaque and free of accountability as the industry it attempts to replace, that is the current food system. The fear is that its core products will be mass-produced, nutritionally dubious meat sold at the homogeneous fast-food joints of the future.[86]

The medical community has warned that gene patents can inhibit the practice of medicine and progress of science.[93] This can also apply to other areas where patents and private intellectual property licenses are being used, often entirely preventing the use and continued development of knowledge and techniques for many years or decades. On the other hand, some worry that without intellectual property protection as the type of R&D-incentive, particularly to current degrees and extents, companies would no longer have the resources or motives/incentives to perform competitive, viable biotech research – as otherwise they may not be able to generate sufficient returns from initial R&D investment or less returns than from other expenditures that are possible.[94] "Biopiracy" refers to "the use of intellectual property systems to legitimize the exclusive ownership and control over biological resources and biological products that have been used over centuries in non-industrialized cultures".[95]

Rather than leading to sustainable, healthy, inexpensive, safe, accessible food being produced with little labor locally – after knowledge- and technology transfer and timely, efficient innovation – the bioeconomy may lead to aggressive monopoly-formation and exacerbated inequality.[96][97][86][additional citation(s) needed] For instance, while production costs may be minimal, costs – including of medicine[98] – may be high.

Innovation management, public spending and governance

See also: Strategic planning

It has been argued that public investment would be a tool governments should use to regulate and license cellular agriculture. Private firms and venture capital would likely seek to maximise investor value rather than social welfare.[86] Moreover, radical innovation is considered to be more risky, "and likely involves more information asymmetry, so that private financial markets may imperfectly manage these frictions". Governments may also help to coordinate "since several innovators may be needed to push the knowledge frontier and make the market profitable, but no single company wants to make the early necessary investments". And investments in the relevant sectors seem to be an bottleneck hindering the transition toward a bioeconomy.[99] Governments could also help innovators that lack the network "to naturally obtain the visibility and political influence necessary to obtain public funds" and could help determine relevant laws.[100] By establishing supporting infrastructure for entrepreneurial ecosystems they can help creating a beneficial environment for innovative bioeconomy startups.[101] Enabling such bioeconomy startups to act on the opportunities provided through the bioeconomy transformation further contributes to its success.[102]


Academic programs

New Harvest Cultured Tissue Fellowship at Tufts University

A joint program between New Harvest and the Tissue Engineering Research Center (TERC), an NIH-supported initiative established in 2004 to advance tissue engineering. The fellowship program offers funding for Masters and PhD students at Tufts university who are interested in bioengineering tunable structures, mechanics, and biology into 3D tissue systems related to their utility as foods.[103]

Conferences

New Harvest Conference

New Harvest brings together pioneers in the cellular agriculture and new, interested parties from industry and academia to share relevant learnings for cellular agriculture's path moving forward. The Conference has been held in San Francisco, California, Brooklyn, New York, and is currently held in Cambridge, Massachusetts.[104]

Industrializing Cell-Based Meats & Seafood Summit

The 3rd Annual Industrializing Cell-Based Meats & Seafood Summit is the only industry-led forum uniting key decision-makers from biotech and food tech, leading food and meat companies, and investors to discuss key operational and technical challenges for the development of cell-based meats and seafood.[105]

International Scientific Conference on Cultured Meat

The International Scientific Conference on Cultured Meat began in collaboration with Maastricht University in 2015, and brings together an international group of scientists and industry experts to present the latest research and developments in cultured meat. It takes place annually in Maastricht, The Netherlands.[106]

Good Food Conference

The GFI conference is an event focused on accelerating the commercialization of plant-based and clean meat.[107]

Cultured Meat Symposium

The Cultured Meat Symposium is a conference held in Silicon Valley highlighting top industry insights of the clean meat revolution.[108][109]

Alternative Protein Show

The Alternative Protein Show is a "networking event" to facilitate collaboration in the "New Protein Landscape", which includes plant-based and cellular agriculture.[110]

New Food Conference

The New Food Conference is an industry-oriented event that aims to accelerate and empower innovative alternatives to animal products by bringing together key stakeholders. It is Europe's first and biggest conference on new-protein solutions.[111]

In the media

Books

  • Clean Meat: How Growing Meat Without Animals Will Revolutionize Dinner and the World is a book about cellular agriculture written by animal activist Paul Shapiro (author). The book reviews startup companies that are currently working towards mass-producing cellular agriculture products.[112][113][114]
  • Meat Planet: Artificial Flesh and the Future of Food by Benjamin Aldes Wurgaft is the result of five years researching cellular agriculture, and explores the quest to generate meat in the lab, asking what it means to imagine that this is the future of food. It is published by the University of California Press.[115]
  • Where do hot dogs come from? A Children's Book about Cellular Agriculture by Anita Broellochs, Alex Shirazi and Illustrated by Gabriel Gonzalez turns a family BBQ into a scientific story explaining how hot dogs are made with cellular agriculture technologies. The book was launched on Kickstarter on July 20, 2021.[116][117]

Podcasts

  • Cultured Meat and Future Food is a podcast about clean meat and future food technologies hosted by Alex Shirazi,[118] a mobile User Experience Designer based in Menlo Park, California, whose current projects focus on retail technology. The podcast features interviews with industry professionals from startups, investors, and non-profits working on cellular agriculture.[119][120]

Similar fields of research and production

See also: Timeline of biotechnology

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External links

  • Overview of relevant bibliography
  • Cellular Agriculture Society

Further reading

  • Clean meat, consumer attitudes and the transition to a cellular agriculture food economy
  • A Closer Look at Cellular Agriculture and the Processes Defining It
  • As lab-grown meat advances, U.S. lawmakers call for regulation
  • CELLULAR AGRICULTURE: A WAY TO FEED TOMORROW’S SMART CITY?
  • Cellular Agriculture, Intentional Imperfection And 'Post Truth': The Transformative Food Trends Of 2017
  • The 4 Key Biotechnologies Needed to Get Cellular Agriculture to Commercialization
  • Cellular agriculture: Growing meat in a lab setting
  • How Might Cellular Agriculture Impact the Livestock, Dairy, and Poultry Industries?

January 03, 2023
cellular, agriculture, focuses, production, agricultural, products, from, cell, cultures, using, combination, biotechnology, tissue, engineering, molecular, biology, synthetic, biology, create, design, methods, producing, proteins, fats, tissues, that, would, . Cellular agriculture focuses on the production of agricultural products from cell cultures using a combination of biotechnology tissue engineering molecular biology and synthetic biology to create and design new methods of producing proteins fats and tissues that would otherwise come from traditional agriculture 1 Most of the industry is focused on animal products such as meat milk and eggs produced in cell culture rather than raising and slaughtering farmed livestock which is associated with substantial global problems of detrimental environmental impacts e g of meat production animal welfare food security and human health 2 3 4 5 Cellular agriculture is field of the biobased economy The most well known cellular agriculture concept is cultured meat source source source source source source source source source source source source source source A video by New Harvest Xprize explaining the development of cultured meat and a post animal bio economy driven by lab grown protein meat eggs milk Runtime 3 09 Contents 1 History 2 Research tools 2 1 Cell lines 2 2 Growth media 2 3 Scaling technologies 2 4 Scaffold materials 2 5 3D tissue systems 3 Applications 3 1 Meat 3 2 Dairy 3 3 Eggs 3 4 Gelatin 3 5 Coffee 3 6 Horseshoe crab blood 3 7 Fish 3 8 Fragrances 3 9 Silk 3 10 Leather 3 11 Pet food 3 12 Wood 4 Issues 4 1 Degrowth green growth and circular economy 4 2 Unemployment and work reallocation 4 3 Patents intellectual property and monopolies 4 4 Innovation management public spending and governance 5 Academic programs 5 1 New Harvest Cultured Tissue Fellowship at Tufts University 6 Conferences 6 1 New Harvest Conference 6 2 Industrializing Cell Based Meats amp Seafood Summit 6 3 International Scientific Conference on Cultured Meat 6 4 Good Food Conference 6 5 Cultured Meat Symposium 6 6 Alternative Protein Show 6 7 New Food Conference 7 In the media 7 1 Books 7 2 Podcasts 8 Similar fields of research and production 9 References 10 External links 11 Further readingHistory EditFor a chronological guide see Timeline of cellular agriculture Although cellular agriculture is a nascent scientific discipline cellular agriculture products were first commercialized in the early 20th century with insulin and rennet 6 On March 24 1990 the FDA approved a bacterium that had been genetically engineered to produce rennet making it the first genetically engineered product for food 7 Rennet is a mixture of enzymes that turns milk into curds and whey in cheese making Traditionally rennet is extracted from the inner lining of the fourth stomach of calves Today cheese making processes use rennet enzymes from genetically engineered bacteria fungi or yeasts because they are unadulterated more consistent and less expensive than animal derived rennet 8 In 2004 Jason Matheny founded New Harvest whose mission is to accelerate breakthroughs in cellular agriculture 9 New Harvest is the only organization focused exclusively on advancing the field of cellular agriculture and provided the first PhD funding specifically for cellular agriculture at Tufts University 10 By 2014 IndieBio a synthetic biology accelerator in San Francisco has incubated several cellular agriculture startups hosting Muufri making milk from cell culture now Perfect Day Foods The EVERY Company making egg whites from cell culture Gelzen making gelatin from bacteria and yeast now Geltor Afineur making cultured coffee beans and Pembient making rhino horn Muufri and The EVERY Company were both initially sponsored by New Harvest In 2015 Mercy for Animals created The Good Food Institute which promotes plant based and cellular agriculture 11 Also in 2015 Isha Datar coined the term cellular agriculture often shortened to cell ag in a New Harvest Facebook group 12 13 On July 13 2016 New Harvest hosted the world s first international conference on cellular agriculture in San Francisco California 9 The day after the conference New Harvest hosted the first closed door workshop for industry academic and government stakeholders in cellular agriculture 14 Research tools EditSeveral key research tools are at the foundation of research in cellular agriculture These include 15 Cell lines Edit A fundamental missing piece in the advancement of cultured meat is the availability of the appropriate cellular materials While some methods and protocols from human and mouse cell culture may apply to agricultural cellular materials it has become clear that most do not This is evidenced by the fact that established protocols for creating human and mouse embryonic stem cells have not succeeded in establishing ungulate embryonic stem cell lines 16 17 18 The ideal criteria for cell lines for the purpose of cultured meat production include immortality high proliferative ability surface independence serum independence and tissue forming ability The specific cell types most suitable for cellular agriculture are likely to differ from species to species 19 20 Growth media Edit Conventional methods for growing animal tissue in culture involves the use of fetal bovine serum FBS FBS is a blood product extracted from fetal calves This product supplies cells with nutrients and stimulating growth factors but is unsustainable and resource heavy to produce with large batch to batch variation 21 Cultured meat companies have been putting significant resources into alternative growth media After the creation of the cell lines efforts to remove serum from the growth media are key to the advancement of cellular agriculture as fetal bovine serum has been the target of most criticisms of cellular agriculture and cultured meat production It is likely that two different media formulations will be required for each cell type a proliferation media for growth and a differentiation media for maturation 22 Scaling technologies Edit As biotechnological processes are scaled experiments start to become increasingly expensive as bioreactors of increasing volume will have to be created Each increase in size will require a re optimization of various parameters such as unit operations fluid dynamics mass transfer and reaction kinetics Scaffold materials Edit For cells to form tissue it is helpful for a material scaffold to be added to provide structure Scaffolds are crucial for cells to form tissues larger than 100 µm across An ideal scaffold must be non toxic for the cells edible and allow for the flow of nutrients and oxygen It must also be cheap and easy to produce on a large scale without the need for animals 3D tissue systems Edit The final phase for creating cultured meat involves bringing together all the previous pieces of research to create large gt 100 µm in diameter pieces of tissue that can be made of mass produced cells without the need for serum where the scaffold is suitable for cells and humans Applications EditWhile the majority of discussion has been around food applications particular cultured meat cellular agriculture can be used to create any kind of agricultural product including those that never involved animals to begin with like Ginkgo Biowork s fragrances Meat Edit Main article Cultured meat Cultured meat also known by other names is a meat produced by in vitro cell cultures of animal cells 23 It is a form of cellular agriculture with such agricultural methods being explored in the context of increased consumer demand for protein 24 Cultured meat is produced using tissue engineering techniques traditionally used in regenerative medicines 25 The concept of cultured meat was introduced to wider audiences by Jason Matheny in the early 2000s after he co authored a paper 26 on cultured meat production and created New Harvest the world s first nonprofit organization dedicated to in vitro meat research 27 Cultured meat may have the potential to address substantial global problems of the environmental impact of meat production animal welfare food security and human health 2 3 4 28 29 Specifically it can be thought of in the context of the mitigation of climate change 24 source source source source source source source source source source source source Lecture The Meat Revolution at the World Economic Forum by Mark Post of the University of Maastricht about in vitro meat runtime 20 16 source source source source source source source source source source source source source source A video by New Harvest Xprize explaining the development of cultured meat and a post animal bio economy driven by lab grown protein meat eggs milk runtime 3 09 In 2013 professor Mark Post at Maastricht University pioneered a proof of concept for cultured meat by creating the first hamburger patty grown directly from cells Since then other cultured meat prototypes have gained media attention SuperMeat opened a farm to fork restaurant called The Chicken 30 in Tel Aviv to test consumer reaction to its Chicken burger 31 while the world s first commercial sale of cell cultured meat occurred in December 2020 at the Singapore restaurant 1880 where cultured meat manufactured by the US firm Eat Just was sold 32 While most efforts in the space focus on common meats such as pork beef and chicken which comprise the bulk of consumption in developed countries 33 some new companies such as Orbillion Bio have focused on high end or unusual meats including Elk Lamb Bison and the prized Wagyu strain of beef 34 Avant Meats has brought cultured grouper fish to market 35 as other companies have started to pursue cultivating additional fish species and other seafood 36 The production process is constantly evolving driven by multiple companies and research institutions 37 The applications of cultured meat have led to ethical health environmental cultural and economic discussions 38 In terms of market strength data published by the non governmental organization Good Food Institute found that in 2021 cultivated meat companies attracted 140 million in Europe alone 24 Currently cultured meat is served at special events and few high end restaurants mass production of cultured meat has not started yet In 2021 researchers presented a bioprinting method to produce steak like cultured meat 39 40 In 2020 the world s first regulatory approval for a cultivated meat product was awarded by the Government of Singapore The chicken meat was grown in a bioreactor in a fluid of amino acids sugar and salt 41 The chicken nuggets food products are 70 lab grown meat while the remainder is made from mung bean proteins and other ingredients The company pledged to strive for price parity with premium restaurant chicken servings 42 43 For an overview of start up companies and non profit organisations working on cellular meat see Cultured meat Companies Dairy Edit Perfect Day is a San Francisco based startup that started as the New Harvest Dairy Project and was incubated by IndieBio in 2014 Perfect Day is making dairy from yeast instead of cows 44 45 The company changed its name from Muufri to Perfect Day in August 2016 46 New Culture is a San Francisco based startup that was incubated by IndieBio in 2019 47 New Culture makes mozzarella cheese using casein protein dairy protein made by microbes instead of cows 48 49 50 Real Vegan Cheese based in the San Francisco Bay area is a grass roots non profit Open Science collective working out of two open community labs and was spun out of the International Genetically Engineered Machine iGEM competition in 2014 51 Real Vegan Cheese are making cheese using casein protein dairy protein made by microbes instead of cows 52 53 54 55 Formo based in Germany is a startup making dairy products using microbial precision fermentation 56 Imagindairy is attempting to create dairy from bioengineered yeast 57 58 Eggs Edit The EVERY Company is a San Francisco based startup that started as the New Harvest Egg Project and was incubated by IndieBio in 2015 The EVERY Company is making egg whites from yeast instead of eggs 59 Gelatin Edit Geltor is a San Francisco based startup that was incubated by IndieBio in 2015 Geltor is developing a proprietary protein production platform that uses bacteria and yeast to produce gelatin 60 61 Coffee Edit In 2021 media outlets reported that the world s first synthetic coffee products have been created by two biotechnology companies still awaiting regulatory approvals for near term commercialization 62 63 64 Such products which can be produced via cellular agriculture in bioreactors 64 and for which multiple companies R amp D have acquired substantial funding may have equal or highly similar effects composition and taste as natural products but use less water generate less carbon emissions require less labor 63 additional citation s needed and cause no deforestation 62 Products that equal naturally grown coffee on the chemical molecular level technically would not be coffee substitutes but differ only in their method of production and hence be lab grown coffee 63 Organizations working on cellular coffee include Afineur is a Brooklyn based startup using biotechnology and smart fermentations to improve the nutritional profile and taste of plant based food starting with craft coffee 65 This list is incomplete you can help by adding missing items October 2021 Horseshoe crab blood Edit Sothic Bioscience is a Cork based startup incubated by IndieBio in 2015 Sothic Bioscience is building a platform for biosynthetic horseshoe crab blood production Horseshoe crab blood contains limulus amebocyte lysate LAL which is the gold standard in validating medical equipment and medication 66 67 Fish Edit Cellular agriculture could be used for commercial fish feed Finless Foods is working to develop and mass manufacture marine animal food products 68 Wild Type is a San Francisco based startup focused on creating cultured meat to address items such as climate change food security and health 69 70 Fragrances Edit Ginkgo Bioworks is a Boston based organism design company culturing fragrances and designing custom microbes 71 Silk Edit This section may contain material unrelated or insufficiently related to the topic of the article Please help improve this section or discuss this issue on the talk page October 2021 Learn how and when to remove this template message See also Similar fields of research and production Spiber is a Japan based company decoding the gene responsible for the production of fibroin in spiders and then bioengineering bacteria with recombinant DNA to produce the protein which they then spin into their artificial silk 72 73 Bolt Threads is a California based company creating engineered silk fibers based on proteins found in spider silk that can be produced at commercial scale Bolt examines the DNA of spiders and then replicates those genetic sequences in other ingredients to create a similar silk fiber Bolt s silk is made primarily of sugar water salts and yeast Through a process called wet spinning this liquid is spun into fiber similar to the way fibers like acrylic and rayon are made 74 75 76 Leather Edit Modern Meadow is a Brooklyn based startup growing collagen a protein found in animal skin to make biofabricated leather 77 Pet food Edit Clean Meat cluster lists Because Animals 78 Wild Earth and Bond Pet Foods 79 as participants in developing pet foods that use cultured meat citation needed Wood Edit In 2022 scientists reported the first 3D printed lab grown wood It is unclear if it could ever be used on a commercial scale e g with sufficient production efficiency and quality 80 81 Issues EditSee also Synthetic biology Ethics Degrowth green growth and circular economy Edit Further information Degrowth The bioeconomy has largely been associated with visions of green growth 82 A study found that a circular bioeconomy may be necessary to build a carbon neutral future in line with the climate objectives of the Paris Agreement 83 However some are concerned that with a focus or reliance on technological progress a fundamentally unsustainable socioeconomic model might be maintained rather than be changed 84 Some are concerned it that may not lead to a ecologization of the economy but to an economization of the biological the living and caution that potentials of non bio based techniques to achieve greater sustainability need to be considered 84 A study found that the as of 2019 current EU interpretation of the bioeconomy is diametrically opposite to the original narrative of Baranoff and Georgescu Roegen that told us that expanding the share of activities based on renewable resources in the economy would slow down economic growth and set strict limits on the overall expansion of the economy 85 Furthermore some caution that Silicon Valley and food corporations could use bioeconomy technologies for greenwashing and monopoly concentrations 86 The bioeconomy its potentials disruptive new modes of production and innovations may distract from the need for systemic structural socioeconomic changes 87 88 and provide a false illusion of technocapitalist utopianism optimism that suggests technological fixes 89 may make it possible to sustain contemporary patterns and structures pre empting structural changes Unemployment and work reallocation Edit Further information Technological unemployment Many farmers depend on conventional methods of producing crops and many of them live in developing economies 90 Cellular agriculture for products such as synthetic coffee could if the contemporary socioeconomic context the socioeconomic system s mechanisms such as incentives and resource distribution mechanisms like markets remains unaltered e g in nature purposes scopes limits and degrees threaten their employment and livelihoods as well as the respective nation s economy and social stability A study concluded that given the expertise required and the high investment costs of the innovation it seems unlikely that cultured meat immediately benefits the poor in developing countries and emphasized that animal agriculture is often essential for the subsistence for farmers in poor countries 91 However not only developing countries may be affected 92 Patents intellectual property and monopolies Edit Observers worry that the bioeconomy will become as opaque and free of accountability as the industry it attempts to replace that is the current food system The fear is that its core products will be mass produced nutritionally dubious meat sold at the homogeneous fast food joints of the future 86 The medical community has warned that gene patents can inhibit the practice of medicine and progress of science 93 This can also apply to other areas where patents and private intellectual property licenses are being used often entirely preventing the use and continued development of knowledge and techniques for many years or decades On the other hand some worry that without intellectual property protection as the type of R amp D incentive particularly to current degrees and extents companies would no longer have the resources or motives incentives to perform competitive viable biotech research as otherwise they may not be able to generate sufficient returns from initial R amp D investment or less returns than from other expenditures that are possible 94 Biopiracy refers to the use of intellectual property systems to legitimize the exclusive ownership and control over biological resources and biological products that have been used over centuries in non industrialized cultures 95 Rather than leading to sustainable healthy inexpensive safe accessible food being produced with little labor locally after knowledge and technology transfer and timely efficient innovation the bioeconomy may lead to aggressive monopoly formation and exacerbated inequality 96 97 86 additional citation s needed For instance while production costs may be minimal costs including of medicine 98 may be high Innovation management public spending and governance Edit See also Strategic planning It has been argued that public investment would be a tool governments should use to regulate and license cellular agriculture Private firms and venture capital would likely seek to maximise investor value rather than social welfare 86 Moreover radical innovation is considered to be more risky and likely involves more information asymmetry so that private financial markets may imperfectly manage these frictions Governments may also help to coordinate since several innovators may be needed to push the knowledge frontier and make the market profitable but no single company wants to make the early necessary investments And investments in the relevant sectors seem to be an bottleneck hindering the transition toward a bioeconomy 99 Governments could also help innovators that lack the network to naturally obtain the visibility and political influence necessary to obtain public funds and could help determine relevant laws 100 By establishing supporting infrastructure for entrepreneurial ecosystems they can help creating a beneficial environment for innovative bioeconomy startups 101 Enabling such bioeconomy startups to act on the opportunities provided through the bioeconomy transformation further contributes to its success 102 Academic programs EditNew Harvest Cultured Tissue Fellowship at Tufts University Edit A joint program between New Harvest and the Tissue Engineering Research Center TERC an NIH supported initiative established in 2004 to advance tissue engineering The fellowship program offers funding for Masters and PhD students at Tufts university who are interested in bioengineering tunable structures mechanics and biology into 3D tissue systems related to their utility as foods 103 Conferences EditNew Harvest Conference Edit New Harvest brings together pioneers in the cellular agriculture and new interested parties from industry and academia to share relevant learnings for cellular agriculture s path moving forward The Conference has been held in San Francisco California Brooklyn New York and is currently held in Cambridge Massachusetts 104 Industrializing Cell Based Meats amp Seafood Summit Edit The 3rd Annual Industrializing Cell Based Meats amp Seafood Summit is the only industry led forum uniting key decision makers from biotech and food tech leading food and meat companies and investors to discuss key operational and technical challenges for the development of cell based meats and seafood 105 International Scientific Conference on Cultured Meat Edit The International Scientific Conference on Cultured Meat began in collaboration with Maastricht University in 2015 and brings together an international group of scientists and industry experts to present the latest research and developments in cultured meat It takes place annually in Maastricht The Netherlands 106 Good Food Conference Edit The GFI conference is an event focused on accelerating the commercialization of plant based and clean meat 107 Cultured Meat Symposium Edit The Cultured Meat Symposium is a conference held in Silicon Valley highlighting top industry insights of the clean meat revolution 108 109 Alternative Protein Show Edit The Alternative Protein Show is a networking event to facilitate collaboration in the New Protein Landscape which includes plant based and cellular agriculture 110 New Food Conference Edit The New Food Conference is an industry oriented event that aims to accelerate and empower innovative alternatives to animal products by bringing together key stakeholders It is Europe s first and biggest conference on new protein solutions 111 In the media EditBooks Edit Clean Meat How Growing Meat Without Animals Will Revolutionize Dinner and the World is a book about cellular agriculture written by animal activist Paul Shapiro author The book reviews startup companies that are currently working towards mass producing cellular agriculture products 112 113 114 Meat Planet Artificial Flesh and the Future of Food by Benjamin Aldes Wurgaft is the result of five years researching cellular agriculture and explores the quest to generate meat in the lab asking what it means to imagine that this is the future of food It is published by the University of California Press 115 Where do hot dogs come from A Children s Book about Cellular Agriculture by Anita Broellochs Alex Shirazi and Illustrated by Gabriel Gonzalez turns a family BBQ into a scientific story explaining how hot dogs are made with cellular agriculture technologies The book was launched on Kickstarter on July 20 2021 116 117 Podcasts Edit Cultured Meat and Future Food is a podcast about clean meat and future food technologies hosted by Alex Shirazi 118 a mobile User Experience Designer based in Menlo Park California whose current projects focus on retail technology The podcast features interviews with industry professionals from startups investors and non profits working on cellular agriculture 119 120 Similar fields of research and production EditSee also Timeline of biotechnology Microbial food cultures and genetically engineered microbial production e g of spider silk 121 122 or solar energy based protein powder 123 124 Controlled self assembly of plant proteins e g of spider silk similar plant proteins based plastics alternatives 125 126 Cell free artificial synthesis see Biobased economy Agriculture Imitation foods e g meat analogues and milk substitutes References Edit A Closer Look at Cellular Agriculture and the Processes Defining It AgFunderNews 2016 07 05 Retrieved 2016 08 05 a b Bryant Christopher J 3 August 2020 Culture meat and cultured meat Journal of Animal Science 98 8 skaa172 doi 10 1093 jas skaa172 ISSN 0021 8812 PMC 7398566 PMID 32745186 a b Hong Tae Kyung Shin Dong Min Choi Joonhyuk Do Jeong Tae Han Sung Gu May 2021 Current Issues and Technical Advances in Cultured Meat Production AReview Food Science of Animal Resources 41 3 355 372 doi 10 5851 kosfa 2021 e14 ISSN 2636 0772 PMC 8112310 PMID 34017947 a b Treich Nicolas 1 May 2021 Cultured Meat Promises and Challenges Environmental 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agriculture food economy A Closer Look at Cellular Agriculture and the Processes Defining It As lab grown meat advances U S lawmakers call for regulation CELLULAR AGRICULTURE A WAY TO FEED TOMORROW S SMART CITY Cellular Agriculture Intentional Imperfection And Post Truth The Transformative Food Trends Of 2017 The 4 Key Biotechnologies Needed to Get Cellular Agriculture to Commercialization Cellular agriculture Growing meat in a lab setting How Might Cellular Agriculture Impact the Livestock Dairy and Poultry Industries Retrieved from https en wikipedia org w index php title Cellular agriculture amp oldid 1123815408, wikipedia, wiki, book, books, library,

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