Vero cells are a lineage of cells used in cell cultures.[1] The 'Vero' lineage was isolated from kidneyepithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species). The lineage was developed on 27 March 1962, by Yasumura and Kawakita at the Chiba University in Chiba, Japan.[2] The original cell line was named Vero after an abbreviation of verda reno, which means 'green kidney' in Esperanto, while vero itself means 'truth' in Esperanto.[3]
The Vero cell lineage is continuous and aneuploid, meaning that it has an abnormal number of chromosomes. A continuous cell lineage can be replicated through many cycles of division and not become senescent.[4] Vero cells are interferon-deficient; unlike normal mammalian cells, they do not secrete interferon alpha or beta when infected by viruses.[5] However, they still have the Interferon-alpha/beta receptor, so they respond normally when recombinant interferon is added to their culture media.
The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014.[6] Chromosome 12 of Vero cells has a homozygous ~9-Mb deletion, causing the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitors CDKN2A and CDKN2B in the genome.[6] Although African green monkeys were previously classified as Cercopithecus aethiops, they have been placed within the genus Chlorocebus, which includes several species.[7] The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus.[6]
as host cells for growing viruses; for example, to measure replication in the presence or absence of a research pharmaceutical, the testing for the presence of rabies virus, or the growth of viral stocks for research purposes. As a recent example, CoronaVac, COVID-19 vaccine developed by Sinovac Biotech uses vero cells in production and "Vero" term can be seen on the vaccine container.
Isolated from Vero in 1968, it grows to a lower saturation density (cells per unit area) than the original Vero. It is useful for detecting and counting hemorrhagic fever viruses by plaque assays.
Vero E6, also known as Vero C1008 (ATCC No. CRL-1586)
This line is a clone from Vero 76. Vero E6 cells show some contact inhibition, so are suitable for propagating viruses that replicate slowly.
Research strains transfected with viral genes:
Vero F6 is a cell transfected with the gene encoding HHV-1entryproteinglycoprotein-H (gH).[8] Vero F6 was transfected via a concatenated plasmid with the gH gene after a copy of the HHV-1 glycoprotein-D (gD) promoter region. In Vero lineage F6, expression of gH is under the control of the promoter region of gD. (Also F6B2; obs. F6B1.1)
^History and Characterization of the Vero Cell Line -- A Report prepared by CDR Rebecca Sheets, Ph.D., USPHS CBER/OVRR/DVRPA/VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12, 2000 OPEN SESSION www.fda.gov pdf
^Yasumura Y, Kawakita M (1963). "The research for the SV40 by means of tissue culture technique". Nippon Rinsho. 21 (6): 1201–1219.
^Shimizu B (1993). Seno K, Koyama H, Kuroki T (eds.). Manual of selected cultured cell lines for bioscience and biotechnology (in Japanese). Tokyo: Kyoritsu Shuppan. pp. 299–300. ISBN978-4-320-05386-1.
^"Main Types of Cell Culture". Fundamental Techniques in Cell Culture: a Laboratory Handbook. Retrieved 2006-09-28.
^Desmyter J, Melnick JL, Rawls WE (October 1968). "Defectiveness of Interferon Production and of Rubella Virus Interference in a Line of African Green Monkey Kidney Cells (Vero)". J. Virol. 2 (10): 955–61. PMC375423. PMID 4302013.
^ abcdeOsada N, Kohara A, Yamaji T, Hirayama N, Kasai F, Sekizuka T, Kuroda M, Hanada K (2014). "The genome landscape of the African green monkey kidney-derived Vero cell line". DNA Research. 21 (6): 673–83. doi:10.1093/dnares/dsu029. PMC4263300. PMID 25267831.
^Haus T, Akom E, Agwanda B, Hofreiter M, Roos C, Zinner D (April 2013). "Mitochondrial diversity and distribution of African green monkeys (chlorocebus gray, 1870)". Am. J. Primatol. 75 (4): 350–60. doi:10.1002/ajp.22113. PMC3613741. PMID 23307319.
^Forrester A, Farrell H, Wilkinson G, Kaye J, Davis-Poynter N, Minson T (January 1992). "Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted". J. Virol. 66 (1): 341–8. PMC238293. PMID 1309250.
External links
Wikimedia Commons has media related to Vero cells.
Cellosaurus entry for Vero
Cellosaurus entry for Vero 76
Cellosaurus entry for Vero C1008
Cellosaurus entry for Vero F6
January 31, 2023
vero, cell, lineage, cells, used, cell, cultures, vero, lineage, isolated, from, kidney, epithelial, cells, extracted, from, african, green, monkey, chlorocebus, formerly, called, cercopithecus, aethiops, this, group, monkeys, been, split, into, several, diffe. Vero cells are a lineage of cells used in cell cultures 1 The Vero lineage was isolated from kidney epithelial cells extracted from an African green monkey Chlorocebus sp formerly called Cercopithecus aethiops this group of monkeys has been split into several different species The lineage was developed on 27 March 1962 by Yasumura and Kawakita at the Chiba University in Chiba Japan 2 The original cell line was named Vero after an abbreviation of verda reno which means green kidney in Esperanto while vero itself means truth in Esperanto 3 Phase contrast microscopic image of Vero cells under green light at 100 fold magnification Contents 1 Characteristics 2 Uses in research 3 Lineages 4 See also 5 References 6 External linksCharacteristics EditThe Vero cell lineage is continuous and aneuploid meaning that it has an abnormal number of chromosomes A continuous cell lineage can be replicated through many cycles of division and not become senescent 4 Vero cells are interferon deficient unlike normal mammalian cells they do not secrete interferon alpha or beta when infected by viruses 5 However they still have the Interferon alpha beta receptor so they respond normally when recombinant interferon is added to their culture media The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014 6 Chromosome 12 of Vero cells has a homozygous 9 Mb deletion causing the loss of the type I interferon gene cluster and cyclin dependent kinase inhibitors CDKN2A and CDKN2B in the genome 6 Although African green monkeys were previously classified as Cercopithecus aethiops they have been placed within the genus Chlorocebus which includes several species 7 The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus 6 Uses in research EditVero cells are used for many purposes including screening for the toxin of Escherichia coli first named Vero toxin after this cell line and later called Shiga like toxin due to its similarity to Shiga toxin isolated from Shigella dysenteriae 6 as host cells for growing viruses for example to measure replication in the presence or absence of a research pharmaceutical the testing for the presence of rabies virus or the growth of viral stocks for research purposes As a recent example CoronaVac COVID 19 vaccine developed by Sinovac Biotech uses vero cells in production and Vero term can be seen on the vaccine container as host cells for eukaryotic parasites specially of the trypanosomatids 6 Lineages EditVero ATCC No CCL 81 Isolated from C aethiops kidney on 27 Mar 1962Vero 76 ATCC No CRL 1587 Isolated from Vero in 1968 it grows to a lower saturation density cells per unit area than the original Vero It is useful for detecting and counting hemorrhagic fever viruses by plaque assays Vero E6 also known as Vero C1008 ATCC No CRL 1586 This line is a clone from Vero 76 Vero E6 cells show some contact inhibition so are suitable for propagating viruses that replicate slowly Research strains transfected with viral genes Vero F6 is a cell transfected with the gene encoding HHV 1 entry protein glycoprotein H gH 8 Vero F6 was transfected via a concatenated plasmid with the gH gene after a copy of the HHV 1 glycoprotein D gD promoter region In Vero lineage F6 expression of gH is under the control of the promoter region of gD Also F6B2 obs F6B1 1 See also EditHeLa cells Immortalised cell lineReferences Edit History and Characterization of the Vero Cell Line A Report prepared by CDR Rebecca Sheets Ph D USPHS CBER OVRR DVRPA VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12 2000 OPEN SESSION www fda gov pdf Yasumura Y Kawakita M 1963 The research for the SV40 by means of tissue culture technique Nippon Rinsho 21 6 1201 1219 Shimizu B 1993 Seno K Koyama H Kuroki T eds Manual of selected cultured cell lines for bioscience and biotechnology in Japanese Tokyo Kyoritsu Shuppan pp 299 300 ISBN 978 4 320 05386 1 Main Types of Cell Culture Fundamental Techniques in Cell Culture a Laboratory Handbook Retrieved 2006 09 28 Desmyter J Melnick JL Rawls WE October 1968 Defectiveness of Interferon Production and of Rubella Virus Interference in a Line of African Green Monkey Kidney Cells Vero J Virol 2 10 955 61 PMC 375423 PMID 4302013 a b c d e Osada N Kohara A Yamaji T Hirayama N Kasai F Sekizuka T Kuroda M Hanada K 2014 The genome landscape of the African green monkey kidney derived Vero cell line DNA Research 21 6 673 83 doi 10 1093 dnares dsu029 PMC 4263300 PMID 25267831 Haus T Akom E Agwanda B Hofreiter M Roos C Zinner D April 2013 Mitochondrial diversity and distribution of African green monkeys chlorocebus gray 1870 Am J Primatol 75 4 350 60 doi 10 1002 ajp 22113 PMC 3613741 PMID 23307319 Forrester A Farrell H Wilkinson G Kaye J Davis Poynter N Minson T January 1992 Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted J Virol 66 1 341 8 PMC 238293 PMID 1309250 External links Edit Wikimedia Commons has media related to Vero cells Cellosaurus entry for Vero Cellosaurus entry for Vero 76 Cellosaurus entry for Vero C1008 Cellosaurus entry for Vero F6 Retrieved from https en wikipedia org w index php title Vero cell amp oldid 1107948202, wikipedia, wiki, book, books, library,
