This gene encodes a member of the tripartite motif family. The encoded protein may function as a [transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[5]
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[9][16] Twenty six tests were carried out on mutant mice and three significant abnormalities were observed.[9] No homozygousmutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; males had increased circulating magnesium levels while animals of both sex displayed increased bone strength.[9]
Referencesedit
^ abcGRCh38: Ensembl release 89: ENSG00000134253 - Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000033233 - Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC3572410. PMID 21677750.
^Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
^Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
^van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC3218837. PMID 21722353.
Further readingedit
Sardiello M, Cairo S, Fontanella B, Ballabio A, Meroni G (2008). "Genomic analysis of the TRIM family reveals two groups of genes with distinct evolutionary properties". BMC Evolutionary Biology. 8 (1): 225. Bibcode:2008BMCEE...8..225S. doi:10.1186/1471-2148-8-225. PMC2533329. PMID 18673550.
Wang Y, Li Y, Qi X, Yuan W, Ai J, Zhu C, Cao L, Yang H, Liu F, Wu X, Liu M (Oct 2004). "TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1". Biochemical and Biophysical Research Communications. 323 (1): 9–16. doi:10.1016/j.bbrc.2004.08.048. PMID 15351693.
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
December 15, 2023
trim45, tripartite, motif, containing, also, known, human, gene, available, structurespdbortholog, search, pdbe, rcsblist, codes2ds4identifiersaliases, rnf99, tripartite, motif, containing, 45external, idsomim, 609318, 1918187, homologene, 11865, genecards, ge. tripartite motif containing 45 also known as TRIM45 is a human gene 5 TRIM45Available structuresPDBOrtholog search PDBe RCSBList of PDB id codes2DS4IdentifiersAliasesTRIM45 RNF99 tripartite motif containing 45External IDsOMIM 609318 MGI 1918187 HomoloGene 11865 GeneCards TRIM45Gene location Human Chr Chromosome 1 human 1 Band1p13 1Start117 111 060 bp 1 End117 122 587 bp 1 Gene location Mouse Chr Chromosome 3 mouse 2 Band3 3 F2 2Start100 829 518 bp 2 End100 844 236 bp 2 RNA expression patternBgeeHumanMouse ortholog Top expressed insecondary oocyteright uterine tubegastrocnemius muscletibialis anterior muscledeltoid musclebody of pancreasbiceps brachiiquadriceps femoris musclevastus lateralis musclethyroid glandTop expressed inotolith organutricleolfactory epitheliumseminiferous tubuleinternal carotid arteryexternal carotid arteryspermatidJacobson s organhandtrigeminal ganglionMore reference expression dataBioGPSn aGene ontologyMolecular functionzinc ion binding metal ion bindingCellular componentintracellular anatomical structure nucleus nucleoplasm intercellular bridge cytoplasm cytosolBiological processbone developmentSources Amigo QuickGOOrthologsSpeciesHumanMouseEntrez80263229644EnsemblENSG00000134253ENSMUSG00000033233UniProtQ9H8W5Q6PFY8RefSeq mRNA NM 001145635NM 025188NM 001165952NM 001165953NM 194343RefSeq protein NP 001139107NP 079464NP 001159424NP 001159425NP 919324Location UCSC Chr 1 117 11 117 12 MbChr 3 100 83 100 84 MbPubMed search 3 4 WikidataView Edit HumanView Edit MouseThis gene encodes a member of the tripartite motif family The encoded protein may function as a transcriptional repressor of the mitogen activated protein kinase pathway Alternatively spliced transcript variants have been described 5 Model organisms editTrim45 knockout mouse phenotype Characteristic PhenotypeHomozygote viability AbnormalRecessive lethal study AbnormalBody weight NormalAnxiety NormalNeurological assessment NormalGrip strength NormalHot plate NormalDysmorphology NormalIndirect calorimetry NormalGlucose tolerance test NormalAuditory brainstem response NormalDEXA NormalRadiography NormalBody temperature NormalEye morphology NormalClinical chemistry Abnormal 6 Plasma immunoglobulins NormalHaematology NormalPeripheral blood lymphocytes NormalMicronucleus test NormalHeart weight NormalTail epidermis wholemount NormalSkin Histopathology NormalBrain histopathology NormalMicroCT amp Quantitative Faxitron AbnormalSalmonella infection Normal 7 Citrobacter infection Normal 8 All tests and analysis from 9 10 Model organisms have been used in the study of TRIM45 function A conditional knockout mouse line called Trim45tm1a KOMP Wtsi 11 12 was generated as part of the International Knockout Mouse Consortium program a high throughput mutagenesis project to generate and distribute animal models of disease to interested scientists 13 14 15 Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion 9 16 Twenty six tests were carried out on mutant mice and three significant abnormalities were observed 9 No homozygous mutant embryos were identified during gestation and therefore none survived until weaning The remaining tests were carried out on heterozygous mutant adult mice males had increased circulating magnesium levels while animals of both sex displayed increased bone strength 9 References edit a b c GRCh38 Ensembl release 89 ENSG00000134253 Ensembl May 2017 a b c GRCm38 Ensembl release 89 ENSMUSG00000033233 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Mouse PubMed Reference National Center for Biotechnology Information U S National Library of Medicine a b Entrez Gene tripartite motif containing 45 Retrieved 2011 08 30 Clinical chemistry data for Trim45 Wellcome Trust Sanger Institute Salmonella infection data for Trim45 Wellcome Trust Sanger Institute Citrobacter infection data for Trim45 Wellcome Trust Sanger Institute a b c d Gerdin AK 2010 The Sanger Mouse Genetics Programme High throughput characterisation of knockout mice Acta Ophthalmologica 88 925 7 doi 10 1111 j 1755 3768 2010 4142 x S2CID 85911512 Mouse Resources Portal Wellcome Trust Sanger Institute International Knockout Mouse Consortium Mouse Genome Informatics Skarnes WC Rosen B West AP Koutsourakis M Bushell W Iyer V Mujica AO Thomas M Harrow J Cox T Jackson D Severin J Biggs P Fu J Nefedov M de Jong PJ Stewart AF Bradley A Jun 2011 A conditional knockout resource for the genome wide study of mouse gene function Nature 474 7351 337 42 doi 10 1038 nature10163 PMC 3572410 PMID 21677750 Dolgin E Jun 2011 Mouse library set to be knockout Nature 474 7351 262 3 doi 10 1038 474262a PMID 21677718 Collins FS Rossant J Wurst W Jan 2007 A mouse for all reasons Cell 128 1 9 13 doi 10 1016 j cell 2006 12 018 PMID 17218247 S2CID 18872015 van der Weyden L White JK Adams DJ Logan DW 2011 The mouse genetics toolkit revealing function and mechanism Genome Biology 12 6 224 doi 10 1186 gb 2011 12 6 224 PMC 3218837 PMID 21722353 Further reading editSardiello M Cairo S Fontanella B Ballabio A Meroni G 2008 Genomic analysis of the TRIM family reveals two groups of genes with distinct evolutionary properties BMC Evolutionary Biology 8 1 225 Bibcode 2008BMCEE 8 225S doi 10 1186 1471 2148 8 225 PMC 2533329 PMID 18673550 Wang Y Li Y Qi X Yuan W Ai J Zhu C Cao L Yang H Liu F Wu X Liu M Oct 2004 TRIM45 a novel human RBCC TRIM protein inhibits transcriptional activities of ElK 1 and AP 1 Biochemical and Biophysical Research Communications 323 1 9 16 doi 10 1016 j bbrc 2004 08 048 PMID 15351693 Maruyama K Sugano S Jan 1994 Oligo capping a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides Gene 138 1 2 171 4 doi 10 1016 0378 1119 94 90802 8 PMID 8125298 Suzuki Y Yoshitomo Nakagawa K Maruyama K Suyama A Sugano S Oct 1997 Construction and characterization of a full length enriched and a 5 end enriched cDNA library Gene 200 1 2 149 56 doi 10 1016 S0378 1119 97 00411 3 PMID 9373149 Retrieved from https en wikipedia org w index php title TRIM45 amp oldid 1187405719, wikipedia, wiki, book, books, library,
