This article is about the surfaces on which organisms live. For enzyme substrates, see Enzyme substrate (biology).
In biology, a substrate is the surface on which an organism (such as a plant, fungus, or animal) lives. A substrate can include biotic or abiotic materials and animals. For example, encrusting algae that lives on a rock (its substrate) can be itself a substrate for an animal that lives on top of the algae. Inert substrates are used as growing support materials in the hydroponic cultivation of plants. In biology substrates are often activated by the nanoscopic process of substrate presentation.
Requirements for animal cell and tissue culture are the same as described for plant cell, tissue and organ culture (In Vitro Culture Techniques: The Biotechnological Principles). Desirable requirements are (i) air conditioning of a room, (ii) hot room with temperature recorder, (iii) microscope room for carrying out microscopic work where different types of microscopes should be installed, (iv) dark room, (v) service room, (vi) sterilization room for sterilization of glassware and culture media, and (vii) preparation room for media preparation, etc. In addition the storage areas should be such where following should be kept properly : (i) liquids-ambient (4-20°C), (ii) glassware-shelving, (iii) plastics-shelving, (iv) small items-drawers, (v) specialized equipments-cupboard, slow turnover, (vi) chemicals-sidled containers.
For cell growth
There are many types of vertebrate cells that require support for their growth in vitro otherwise they will not grow properly. Such cells are called anchorage-dependent cells. Therefore, many substrates which may be adhesive (e.g. plastic, glass, palladium, metallic surfaces, etc.) or non-adhesive (e.g. agar, agarose, etc.) types may be used as discussed below:
Plastic as a substrate. Disposable plastics are cheaper substrate as they are commonly made up of polystyrene. After use they should be disposed of properly. Before use they are treated with gamma radiation or electric arc simply to develop charges on the surface of substrate. After cell growth its rate of proliferation should be measured. In addition, the other plastic materials used as substrate are teflon or polytetrafluoroethylene (PTFE), thermamox (TPX), polyvinylchloride (PVC), polycarbonate, etc. Monolayer of cell must be grown. Moreover, plastic beads of polystyrene, sephadex and polyacrylamide are also available for cell growth in suspension culture.
Glass as a substrate. Glass is an important substrate used in laboratory in several forms such as test tubes, slides, coverslips, pipettes, flasks, rods, bottles, Petri dishes, several apparatus, etc. These are sterilized by using chemicals, radiations, dry heat (in oven) and moist heat (in autoclave).
Palladium as a substrate. For the first time palladium deposited on agarose was used as a substrate for growth of fibroblast and glia. [2]
^Biocyclopedia.com. "Requirements for Animal Cell and Tissue Culture - Animal Biotech". biocyclopedia.com. Retrieved 2018-03-13.
External links
"Micro-vegetable growing" using abiotic substrates at home
February 10, 2023
substrate, biology, this, article, about, surfaces, which, organisms, live, enzyme, substrates, enzyme, substrate, biology, biology, substrate, surface, which, organism, such, plant, fungus, animal, lives, substrate, include, biotic, abiotic, materials, animal. This article is about the surfaces on which organisms live For enzyme substrates see Enzyme substrate biology In biology a substrate is the surface on which an organism such as a plant fungus or animal lives A substrate can include biotic or abiotic materials and animals For example encrusting algae that lives on a rock its substrate can be itself a substrate for an animal that lives on top of the algae Inert substrates are used as growing support materials in the hydroponic cultivation of plants In biology substrates are often activated by the nanoscopic process of substrate presentation Contents 1 In agriculture and horticulture 2 In animal biotechnology 2 1 Requirements for animal cell and tissue culture 2 2 For cell growth 3 References 4 External linksIn agriculture and horticulture EditCellulose substrate 1 Expanded clay aggregate LECA Rock wool Potting soil SoilIn animal biotechnology EditRequirements for animal cell and tissue culture Edit Requirements for animal cell and tissue culture are the same as described for plant cell tissue and organ culture In Vitro Culture Techniques The Biotechnological Principles Desirable requirements are i air conditioning of a room ii hot room with temperature recorder iii microscope room for carrying out microscopic work where different types of microscopes should be installed iv dark room v service room vi sterilization room for sterilization of glassware and culture media and vii preparation room for media preparation etc In addition the storage areas should be such where following should be kept properly i liquids ambient 4 20 C ii glassware shelving iii plastics shelving iv small items drawers v specialized equipments cupboard slow turnover vi chemicals sidled containers For cell growth Edit There are many types of vertebrate cells that require support for their growth in vitro otherwise they will not grow properly Such cells are called anchorage dependent cells Therefore many substrates which may be adhesive e g plastic glass palladium metallic surfaces etc or non adhesive e g agar agarose etc types may be used as discussed below Plastic as a substrate Disposable plastics are cheaper substrate as they are commonly made up of polystyrene After use they should be disposed of properly Before use they are treated with gamma radiation or electric arc simply to develop charges on the surface of substrate After cell growth its rate of proliferation should be measured In addition the other plastic materials used as substrate are teflon or polytetrafluoroethylene PTFE thermamox TPX polyvinylchloride PVC polycarbonate etc Monolayer of cell must be grown Moreover plastic beads of polystyrene sephadex and polyacrylamide are also available for cell growth in suspension culture Glass as a substrate Glass is an important substrate used in laboratory in several forms such as test tubes slides coverslips pipettes flasks rods bottles Petri dishes several apparatus etc These are sterilized by using chemicals radiations dry heat in oven and moist heat in autoclave Palladium as a substrate For the first time palladium deposited on agarose was used as a substrate for growth of fibroblast and glia 2 References Edit Cellulose substrate being used to grow plants Biocyclopedia com Requirements for Animal Cell and Tissue Culture Animal Biotech biocyclopedia com Retrieved 2018 03 13 External links Edit Micro vegetable growing using abiotic substrates at home Retrieved from https en wikipedia org w index php title Substrate biology amp oldid 1080122457, wikipedia, wiki, book, books, library,
