In chemical analysis, matrix refers to the components of a sample other than the analyte[1] of interest. The matrix can have a considerable effect on the way the analysis is conducted and the quality of the results are obtained; such effects are called matrix effects.[2] For example, the ionic strength of the solution can have an effect on the activity coefficients of the analytes.[3][4] The most common approach for accounting for matrix effects is to build a calibration curve using standard samples with known analyte concentration and which try to approximate the matrix of the sample as much as possible.[2] This is especially important for solid samples where there is a strong matrix influence.[5] In cases with complex or unknown matrices, the standard addition method can be used.[3] In this technique, the response of the sample is measured and recorded, for example, using an electrode selective for the analyte. Then, a small volume of standard solution is added and the response is measured again. Ideally, the standard addition should increase the analyte concentration by a factor of 1.5 to 3, and several additions should be averaged. The volume of standard solution should be small enough to disturb the matrix as little as possible.
Matrix effectedit
Matrix enhancement and suppression is frequently observed in modern analytical routines, such as GC, HPLC, and ICP.
Matrix effect is quantitated by the use of the following formula:
where
A(extract) is the peak area of analyte, when diluted with matrix extract.
A(standard) is the peak area of analyte in the absence of matrix.
The concentration of analyte in both standards should be the same. A matrix effect value close to 100 indicates absence of matrix influence. A matrix effect value of less than 100 indicates suppression, while a value larger than 100 is a sign of matrix enhancement.
An alternative definition of matrix effect utilizes the formula:
The advantages of this definition are that negative values indicates suppression, while positive values are a sign of matrix enhancement. Ideally, a value of 0 is related to the absence of matrix effect.
^Marco Aurelio Zezzi Arruda. Trends in Sample Preparation. Nova Publishers, 2006, p. 15-18. ISBN1-60021-118-6.
January 01, 1970
matrix, chemical, analysis, chemical, analysis, matrix, refers, components, sample, other, than, analyte, interest, matrix, have, considerable, effect, analysis, conducted, quality, results, obtained, such, effects, called, matrix, effects, example, ionic, str. In chemical analysis matrix refers to the components of a sample other than the analyte 1 of interest The matrix can have a considerable effect on the way the analysis is conducted and the quality of the results are obtained such effects are called matrix effects 2 For example the ionic strength of the solution can have an effect on the activity coefficients of the analytes 3 4 The most common approach for accounting for matrix effects is to build a calibration curve using standard samples with known analyte concentration and which try to approximate the matrix of the sample as much as possible 2 This is especially important for solid samples where there is a strong matrix influence 5 In cases with complex or unknown matrices the standard addition method can be used 3 In this technique the response of the sample is measured and recorded for example using an electrode selective for the analyte Then a small volume of standard solution is added and the response is measured again Ideally the standard addition should increase the analyte concentration by a factor of 1 5 to 3 and several additions should be averaged The volume of standard solution should be small enough to disturb the matrix as little as possible Matrix effect editMatrix enhancement and suppression is frequently observed in modern analytical routines such as GC HPLC and ICP Matrix effect is quantitated by the use of the following formula M E 100 A e x t r a c t A s t a n d a r d displaystyle ME 100 left frac A extract A standard right nbsp whereA extract is the peak area of analyte when diluted with matrix extract A standard is the peak area of analyte in the absence of matrix The concentration of analyte in both standards should be the same A matrix effect value close to 100 indicates absence of matrix influence A matrix effect value of less than 100 indicates suppression while a value larger than 100 is a sign of matrix enhancement An alternative definition of matrix effect utilizes the formula M E 100 A e x t r a c t A s t a n d a r d 100 displaystyle ME 100 left frac A extract A standard right 100 nbsp The advantages of this definition are that negative values indicates suppression while positive values are a sign of matrix enhancement Ideally a value of 0 is related to the absence of matrix effect See also editExtraction chemistry References edit IUPAC Compendium of Chemical Terminology 2nd ed the Gold Book 1997 Online corrected version 1989 Matrix in analysis doi 10 1351 goldbook M03758 a b F W Fifield P J Haines Environmental Analytical Chemistry Blackwell Publishing 2000 p 4 5 ISBN 0 632 05383 6 a b Harris D C Quantitative Chemical Analysis 4th ed Freeman 1995 pp 194 404 ISBN 0 7167 2508 8 IUPAC Compendium of Chemical Terminology 2nd ed the Gold Book 1997 Online corrected version 1989 Matrix effect doi 10 1351 goldbook M03759 Marco Aurelio Zezzi Arruda Trends in Sample Preparation Nova Publishers 2006 p 15 18 ISBN 1 60021 118 6 Retrieved from https en wikipedia org w index php title Matrix chemical analysis amp oldid 1133503550, wikipedia, wiki, book, books, library,
