Nuclear pore glycoprotein-210 (gp210) is an essential trafficking regulator in the eukaryotic nuclear pore complex. Gp-210 anchors the pore complex to the nuclear membrane.[5] and protein tagging reveals its primarily located on the luminal side of double layer membrane at the pore. A single polypeptide motif of gp210 is responsible for sorting to nuclear membrane,[6] and indicate the carboxyl tail of the protein is oriented toward the cytoplasmic side of the membrane.
During eukaryoticmitosis the nuclear envelope disintegrates into vesicles dispersing nuclear lamina proteins and nuclear pore complexes. Nup210 is specifically phosphorylated on the C-terminal (cytoplasmic) domain in mitosis at Ser1880[7] and is dispersed throughout the endoplasmic reticulum during mitosis as homodimers.[8] Nuclear lamins begin to reassemble around chromosomes at the end of mitosis.[9] Nup210 lags the reassembly process relative to other Nups.[10] and while much of the assembly process can occur without it, the final assembly and dilation of the complexes require Nup210.[11] The replacement of serine at position 1880 with a phosphorylated 'looking' glutamate results in Nup210 complexes that fail to reassemble indicating that dephosphorylation of Nup210 within the final phases of proper assembly is required.[12]
Pathologyedit
Recognized by anti-nuclear antibodies found in primary biliary cirrhosis (PBC) anti-Nup210 antibodies correlate with progression toward end stage liver disease. Nup210 is possibly a destructive autoimmune target of the disease. One idea for the loss of tolerance is the increased or abnormal expression of Nup210 in patients with PBC.[13]
^ abcGRCh38: Ensembl release 89: ENSG00000132182 - Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000030091 - Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Greber UF, Senior A, Gerace L (1990). "A major glycoprotein of the nuclear pore complex is a membrane-spanning polypeptide with a large lumenal domain and a small cytoplasmic tail". EMBO J. 9 (5): 1495–502. doi:10.1002/j.1460-2075.1990.tb08267.x. PMC551841. PMID 2184032.
^Wozniak RW, Blobel G (1992). "The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope". J. Cell Biol. 119 (6): 1441–9. doi:10.1083/jcb.119.6.1441. PMC2289754. PMID 1281815.
^Favreau C, Worman HJ, Wozniak RW, Frappier T, Courvalin JC (1996). "Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210". Biochemistry. 35 (24): 8035–44. doi:10.1021/bi9600660. PMID 8672508.
^Favreau C, Bastos R, Cartaud J, Courvalin JC, Mustonen P (2001). "Biochemical characterization of nuclear pore complex protein gp210 oligomers". Eur. J. Biochem. 268 (14): 3883–9. doi:10.1046/j.1432-1327.2001.02290.x. PMID 11453980.
^Yang L, Guan T, Gerace L (1997). "Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis". J. Cell Biol. 137 (6): 1199–210. doi:10.1083/jcb.137.6.1199. PMC2132536. PMID 9182656.
^Bodoor K, Shaikh S, Salina D, et al. (1999). "Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis". J. Cell Sci. 112 (13): 2253–64. doi:10.1242/jcs.112.13.2253. PMID 10362555.
^Cohen M, Feinstein N, Wilson KL, Gruenbaum Y (2003). "Nuclear pore protein gp210 is essential for viability in HeLa cells and Caenorhabditis elegans". Mol. Biol. Cell. 14 (10): 4230–7. doi:10.1091/mbc.E03-04-0260. PMC207014. PMID 14517331.
^Onischenko EA, Crafoord E, Hallberg E (2007). "Phosphomimetic mutation of the mitotically phosphorylated serine 1880 compromises the interaction of the transmembrane nucleoporin gp210 with the nuclear pore complex". Exp. Cell Res. 313 (12): 2744–51. doi:10.1016/j.yexcr.2007.05.011. hdl:10616/41278. PMID 17559836.
^Nakamura M, Takii Y, Ito M, et al. (2006). "Increased expression of nuclear envelope gp210 antigen in small bile ducts in primary biliary cirrhosis". J. Autoimmun. 26 (2): 138–45. doi:10.1016/j.jaut.2005.10.007. PMID 16337775.
April 11, 2024
nucleoporin, 210kda, nuclear, pore, glycoprotein, gp210, essential, trafficking, regulator, eukaryotic, nuclear, pore, complex, anchors, pore, complex, nuclear, membrane, protein, tagging, reveals, primarily, located, luminal, side, double, layer, membrane, po. Nuclear pore glycoprotein 210 gp210 is an essential trafficking regulator in the eukaryotic nuclear pore complex Gp 210 anchors the pore complex to the nuclear membrane 5 and protein tagging reveals its primarily located on the luminal side of double layer membrane at the pore A single polypeptide motif of gp210 is responsible for sorting to nuclear membrane 6 and indicate the carboxyl tail of the protein is oriented toward the cytoplasmic side of the membrane Nucleoporin 210kDaIdentifiersAliasesNUP210 Nup210 GP210 POM210 nucleoporin 210kDa nucleoporin 210External IDsOMIM 607703 MGI 1859555 HomoloGene 41286 GeneCards NUP210Gene location Human Chr Chromosome 3 human 1 Band3p25 1Start13 316 235 bp 1 End13 420 309 bp 1 Gene location Mouse Chr Chromosome 6 mouse 2 Band6 D1 6 40 3 cMStart90 990 050 bp 2 End91 093 811 bp 2 RNA expression patternBgeeHumanMouse ortholog Top expressed inlymph nodebone marrow cellsthymusspleenbloodappendixparotid glandmonocytespongy boneright lobe of liverTop expressed inspleenbloodthymushair folliclebone marrowPaneth cellcumulus cellsomitetemporal muscletriceps brachii muscleMore reference expression dataBioGPSn aGene ontologyMolecular functionprotein dimerization activityCellular componentnuclear membrane integral component of membrane nucleus nuclear envelope membrane endoplasmic reticulum endoplasmic reticulum membrane nuclear pore host cellBiological processmitotic nuclear membrane disassembly viral transcription viral process protein sumoylation protein transport mRNA transport regulation of cellular response to heat intracellular transport of virus mRNA export from nucleus tRNA export from nucleus regulation of gene silencing by miRNA regulation of glycolytic process transportSources Amigo QuickGOOrthologsSpeciesHumanMouseEntrez2322554563EnsemblENSG00000132182ENSMUSG00000030091UniProtQ8TEM1Q9QY81RefSeq mRNA NM 024923NM 018815RefSeq protein NP 079199NP 061285Location UCSC Chr 3 13 32 13 42 MbChr 6 90 99 91 09 MbPubMed search 3 4 WikidataView Edit HumanView Edit MouseDisassembly and Assembly editDuring eukaryotic mitosis the nuclear envelope disintegrates into vesicles dispersing nuclear lamina proteins and nuclear pore complexes Nup210 is specifically phosphorylated on the C terminal cytoplasmic domain in mitosis at Ser1880 7 and is dispersed throughout the endoplasmic reticulum during mitosis as homodimers 8 Nuclear lamins begin to reassemble around chromosomes at the end of mitosis 9 Nup210 lags the reassembly process relative to other Nups 10 and while much of the assembly process can occur without it the final assembly and dilation of the complexes require Nup210 11 The replacement of serine at position 1880 with a phosphorylated looking glutamate results in Nup210 complexes that fail to reassemble indicating that dephosphorylation of Nup210 within the final phases of proper assembly is required 12 Pathology editRecognized by anti nuclear antibodies found in primary biliary cirrhosis PBC anti Nup210 antibodies correlate with progression toward end stage liver disease Nup210 is possibly a destructive autoimmune target of the disease One idea for the loss of tolerance is the increased or abnormal expression of Nup210 in patients with PBC 13 Anti mitochondrial anti centromere and anti nup62 are also found in PBC References edit a b c GRCh38 Ensembl release 89 ENSG00000132182 Ensembl May 2017 a b c GRCm38 Ensembl release 89 ENSMUSG00000030091 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Mouse PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Greber UF Senior A Gerace L 1990 A major glycoprotein of the nuclear pore complex is a membrane spanning polypeptide with a large lumenal domain and a small cytoplasmic tail EMBO J 9 5 1495 502 doi 10 1002 j 1460 2075 1990 tb08267 x PMC 551841 PMID 2184032 Wozniak RW Blobel G 1992 The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope J Cell Biol 119 6 1441 9 doi 10 1083 jcb 119 6 1441 PMC 2289754 PMID 1281815 Favreau C Worman HJ Wozniak RW Frappier T Courvalin JC 1996 Cell cycle dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210 Biochemistry 35 24 8035 44 doi 10 1021 bi9600660 PMID 8672508 Favreau C Bastos R Cartaud J Courvalin JC Mustonen P 2001 Biochemical characterization of nuclear pore complex protein gp210 oligomers Eur J Biochem 268 14 3883 9 doi 10 1046 j 1432 1327 2001 02290 x PMID 11453980 Yang L Guan T Gerace L 1997 Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis J Cell Biol 137 6 1199 210 doi 10 1083 jcb 137 6 1199 PMC 2132536 PMID 9182656 Bodoor K Shaikh S Salina D et al 1999 Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis J Cell Sci 112 13 2253 64 doi 10 1242 jcs 112 13 2253 PMID 10362555 Cohen M Feinstein N Wilson KL Gruenbaum Y 2003 Nuclear pore protein gp210 is essential for viability in HeLa cells and Caenorhabditis elegans Mol Biol Cell 14 10 4230 7 doi 10 1091 mbc E03 04 0260 PMC 207014 PMID 14517331 Onischenko EA Crafoord E Hallberg E 2007 Phosphomimetic mutation of the mitotically phosphorylated serine 1880 compromises the interaction of the transmembrane nucleoporin gp210 with the nuclear pore complex Exp Cell Res 313 12 2744 51 doi 10 1016 j yexcr 2007 05 011 hdl 10616 41278 PMID 17559836 Nakamura M Takii Y Ito M et al 2006 Increased expression of nuclear envelope gp210 antigen in small bile ducts in primary biliary cirrhosis J Autoimmun 26 2 138 45 doi 10 1016 j jaut 2005 10 007 PMID 16337775 Retrieved from https en wikipedia org w index php title Nucleoporin 210kDa amp oldid 1116918783, wikipedia, wiki, book, books, library,
