The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria primarily in liver and kidney. The glycine N-acyltransferase enzyme is involved in the detoxification of a wide range of xenobiotic and endogenousmetabolites. These include benzoic acid, a compound found in fruits and vegetables and used in medicine and foodstuffs as a preservative; salicylic acid, a metabolite of aspirin; and several endogenous metabolites. The diversity is demonstrated by the wide range of acylglycines excreted in the urines of patients with defects of organic acid metabolism. No defect of glycine N-acyltransferase has yet been described, but it has been demonstrated that there is significant inter individual variation in glycine conjugation capacity. Human glycine N-acyltransferase isoform a is a 296 amino acid protein translated from mRNA transcript splice variant 1. It is encoded by exons 2 to 6 of the mRNA transcript.[5]
Molecular weightedit
The literature reports it to be approximately 30 kDa,[6] or approximately 27 kDa.[7] The predicted size is 33.9 KDa. For the bovine enzyme a range of sizes between approximately 33 kDa and about 36 KDa is reported (Nandi, 1979, Vessey, 1992, van der Westhuizen, 2000). The predicted size of bovine GLYAT based on its sequence (accession number nm: 177486), is 33.9 kDa. This compares well to the experimentally determined sizes[7][8][9]
Referencesedit
^ abcGRCh38: Ensembl release 89: ENSG00000149124 – Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000063683 – Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ abMawal YR, Qureshi IA (December 1994). "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver". Biochem. Biophys. Res. Commun. 205 (2): 1373–9. doi:10.1006/bbrc.1994.2817. PMID 7802672.
^ abvan der Westhuizen FH, Pretorius PJ, Erasmus E (2000). "The utilization of alanine, glutamic acid, and serine as amino acid substrates for glycine N-acyltransferase". J. Biochem. Mol. Toxicol. 14 (2): 102–9. doi:10.1002/(SICI)1099-0461(2000)14:2<102::AID-JBT6>3.0.CO;2-H. PMID 10630424. S2CID 44672034.
^Nandi DL, Lucas SV, Webster LT (August 1979). "Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization". J. Biol. Chem. 254 (15): 7230–7. doi:10.1016/S0021-9258(18)50309-4. PMID 457678.
^Kelley M, Vessey DA (November 1992). "Structural comparison between the mitochondrial aralkyl-CoA and arylacetyl-CoA N-acyltransferases". Biochem. J. 288 (1): 315–7. doi:10.1042/bj2880315. PMC1132116. PMID 1445276.
Further readingedit
Webster LT, Siddiqui UA, Lucas SV, et al. (1976). "Identification of separate acyl- CoA:glycine and acyl-CoA:L-glutamine N-acyltransferase activities in mitochondrial fractions from liver of rhesus monkey and man". J. Biol. Chem. 251 (11): 3352–8. doi:10.1016/S0021-9258(17)33444-0. PMID 931988.
Mawal YR, Qureshi IA (1994). "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver". Biochem. Biophys. Res. Commun. 205 (2): 1373–9. doi:10.1006/bbrc.1994.2817. PMID 7802672.
Mawal YR, Qureshi IA (1995). "An immunodetection method for the quantitation of human acyl CoA:glycine N-acyltransferase in biological samples". Biochem. Mol. Biol. Int. 34 (3): 595–601. PMID 7833837.
Merkler DJ, Merkler KA, Stern W, Fleming FF (1996). "Fatty acid amide biosynthesis: a possible new role for peptidylglycine alpha-amidating enzyme and acyl-coenzyme A: glycine N-acyltransferase". Arch. Biochem. Biophys. 330 (2): 430–4. doi:10.1006/abbi.1996.0272. PMID 8660675.
Mawal Y, Paradis K, Qureshi IA (1997). "Developmental profile of mitochondrial glycine N-acyltransferase in human liver". J. Pediatr. 130 (6): 1003–7. doi:10.1016/S0022-3476(97)70293-2. PMID 9202629.
van der Westhuizen FH, Pretorius PJ, Erasmus E (2000). "The utilization of alanine, glutamic acid, and serine as amino acid substrates for glycine N-acyltransferase". J. Biochem. Mol. Toxicol. 14 (2): 102–9. doi:10.1002/(SICI)1099-0461(2000)14:2<102::AID-JBT6>3.0.CO;2-H. PMID 10630424. S2CID 44672034.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC139241. PMID 12477932.
SCHACHTER D, TAGGART JV (2003). "Glycine N-acylase: purification and properties". J. Biol. Chem. 208 (1): 263–75. doi:10.1016/S0021-9258(18)65643-1. PMID 13174534.
Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC515316. PMID 15342556.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC528928. PMID 15489334.
glyat, glycine, acyltransferase, also, known, enzyme, which, humans, encoded, gene, identifiersaliases, acgnat, glycine, acyltransferaseexternal, idsomim, 607424, 2147502, homologene, 64840, genecards, gene, location, human, chromosome, human, band11q12, 1star. Glycine N acyltransferase also known as GLYAT is an enzyme which in humans is encoded by the GLYAT gene 5 6 GLYATIdentifiersAliasesGLYAT ACGNAT CAT GAT glycine N acyltransferaseExternal IDsOMIM 607424 MGI 2147502 HomoloGene 64840 GeneCards GLYATGene location Human Chr Chromosome 11 human 1 Band11q12 1Start58 640 426 bp 1 End58 731 974 bp 1 Gene location Mouse Chr Chromosome 19 mouse 2 Band19 19 AStart12 610 672 bp 2 End12 631 275 bp 2 RNA expression patternBgeeHumanMouse ortholog Top expressed inright lobe of liverkidney tubulekidneyglomerulusmetanephric glomerulusrenal medullasubcutaneous adipose tissuesural nerveabdominal fattibialis anterior muscleTop expressed inkidneyleft lobe of liverproximal tubulefacial motor nucleusanterior horn of spinal cordcrypt of lieberkuhn of small intestineintestinal villusyolk sacskin of abdomenileumMore reference expression dataBioGPSMore reference expression dataGene ontologyMolecular functiontransferase activity acyltransferase activity protein binding glycine N acyltransferase activity glycine N benzoyltransferase activityCellular componentmitochondrial matrix mitochondrionBiological processxenobiotic metabolic process acyl CoA metabolic process monocarboxylic acid metabolic process response to toxic substance glycine metabolic process benzoyl CoA metabolic processSources Amigo QuickGOOrthologsSpeciesHumanMouseEntrez10249107146EnsemblENSG00000149124ENSMUSG00000063683UniProtQ6IB77Q91XE0RefSeq mRNA NM 201648NM 005838NM 145935RefSeq protein NP 005829NP 964011NP 666047Location UCSC Chr 11 58 64 58 73 MbChr 19 12 61 12 63 MbPubMed search 3 4 WikidataView Edit HumanView Edit Mouse Contents 1 Function 2 Molecular weight 3 References 4 Further reading 5 External linksFunction editThe glycine N acyltransferase protein conjugates glycine with acyl CoA substrates in the mitochondria primarily in liver and kidney The glycine N acyltransferase enzyme is involved in the detoxification of a wide range of xenobiotic and endogenous metabolites These include benzoic acid a compound found in fruits and vegetables and used in medicine and foodstuffs as a preservative salicylic acid a metabolite of aspirin and several endogenous metabolites The diversity is demonstrated by the wide range of acylglycines excreted in the urines of patients with defects of organic acid metabolism No defect of glycine N acyltransferase has yet been described but it has been demonstrated that there is significant inter individual variation in glycine conjugation capacity Human glycine N acyltransferase isoform a is a 296 amino acid protein translated from mRNA transcript splice variant 1 It is encoded by exons 2 to 6 of the mRNA transcript 5 Molecular weight editThe literature reports it to be approximately 30 kDa 6 or approximately 27 kDa 7 The predicted size is 33 9 KDa For the bovine enzyme a range of sizes between approximately 33 kDa and about 36 KDa is reported Nandi 1979 Vessey 1992 van der Westhuizen 2000 The predicted size of bovine GLYAT based on its sequence accession number nm 177486 is 33 9 kDa This compares well to the experimentally determined sizes 7 8 9 References edit a b c GRCh38 Ensembl release 89 ENSG00000149124 Ensembl May 2017 a b c GRCm38 Ensembl release 89 ENSMUSG00000063683 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Mouse PubMed Reference National Center for Biotechnology Information U S National Library of Medicine a b Entrez Gene GLYAT glycine N acyltransferase a b Mawal YR Qureshi IA December 1994 Purification to homogeneity of mitochondrial acyl coa glycine n acyltransferase from human liver Biochem Biophys Res Commun 205 2 1373 9 doi 10 1006 bbrc 1994 2817 PMID 7802672 a b van der Westhuizen FH Pretorius PJ Erasmus E 2000 The utilization of alanine glutamic acid and serine as amino acid substrates for glycine N acyltransferase J Biochem Mol Toxicol 14 2 102 9 doi 10 1002 SICI 1099 0461 2000 14 2 lt 102 AID JBT6 gt 3 0 CO 2 H PMID 10630424 S2CID 44672034 Nandi DL Lucas SV Webster LT August 1979 Benzoyl coenzyme A glycine N acyltransferase and phenylacetyl coenzyme A glycine N acyltransferase from bovine liver mitochondria Purification and characterization J Biol Chem 254 15 7230 7 doi 10 1016 S0021 9258 18 50309 4 PMID 457678 Kelley M Vessey DA November 1992 Structural comparison between the mitochondrial aralkyl CoA and arylacetyl CoA N acyltransferases Biochem J 288 1 315 7 doi 10 1042 bj2880315 PMC 1132116 PMID 1445276 Further reading editWebster LT Siddiqui UA Lucas SV et al 1976 Identification of separate acyl CoA glycine and acyl CoA L glutamine N acyltransferase activities in mitochondrial fractions from liver of rhesus monkey and man J Biol Chem 251 11 3352 8 doi 10 1016 S0021 9258 17 33444 0 PMID 931988 Mawal YR Qureshi IA 1994 Purification to homogeneity of mitochondrial acyl coa glycine n acyltransferase from human liver Biochem Biophys Res Commun 205 2 1373 9 doi 10 1006 bbrc 1994 2817 PMID 7802672 Mawal YR Qureshi IA 1995 An immunodetection method for the quantitation of human acyl CoA glycine N acyltransferase in biological samples Biochem Mol Biol Int 34 3 595 601 PMID 7833837 Merkler DJ Merkler KA Stern W Fleming FF 1996 Fatty acid amide biosynthesis a possible new role for peptidylglycine alpha amidating enzyme and acyl coenzyme A glycine N acyltransferase Arch Biochem Biophys 330 2 430 4 doi 10 1006 abbi 1996 0272 PMID 8660675 Mawal Y Paradis K Qureshi IA 1997 Developmental profile of mitochondrial glycine N acyltransferase in human liver J Pediatr 130 6 1003 7 doi 10 1016 S0022 3476 97 70293 2 PMID 9202629 van der Westhuizen FH Pretorius PJ Erasmus E 2000 The utilization of alanine glutamic acid and serine as amino acid substrates for glycine N acyltransferase J Biochem Mol Toxicol 14 2 102 9 doi 10 1002 SICI 1099 0461 2000 14 2 lt 102 AID JBT6 gt 3 0 CO 2 H PMID 10630424 S2CID 44672034 Strausberg RL Feingold EA Grouse LH et al 2003 Generation and initial analysis of more than 15 000 full length human and mouse cDNA sequences Proc Natl Acad Sci U S A 99 26 16899 903 Bibcode 2002PNAS 9916899M doi 10 1073 pnas 242603899 PMC 139241 PMID 12477932 SCHACHTER D TAGGART JV 2003 Glycine N acylase purification and properties J Biol Chem 208 1 263 75 doi 10 1016 S0021 9258 18 65643 1 PMID 13174534 Suzuki Y Yamashita R Shirota M et al 2004 Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions Genome Res 14 9 1711 8 doi 10 1101 gr 2435604 PMC 515316 PMID 15342556 Gerhard DS Wagner L Feingold EA et al 2004 The status quality and expansion of the NIH full length cDNA project the Mammalian Gene Collection MGC Genome Res 14 10B 2121 7 doi 10 1101 gr 2596504 PMC 528928 PMID 15489334 External links editGLYAT human gene location in the UCSC Genome Browser GLYAT human gene details in the UCSC Genome Browser nbsp This article on a gene on human chromosome 11 is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title GLYAT amp oldid 1194297006, wikipedia, wiki, book, books, library,
