Historically, fluorophores such as fluorescein, rhodamine, Cy3 and Cy5 have been used in a wide variety of applications. These dyes have limitations for use in microscopy and other applications that require exposure to an intense light source such as a laser, because they photobleach quickly (however, bleaching can be reduced at least 10 fold using oxygen scavenging). DyLight Fluors have comparable excitation and emission spectra and are claimed to be more photostable, brighter, and less pH-sensitive. The excitation and emission spectra of the DyLight Fluor series cover much of the visible spectrum and extend into the infrared region, allowing detection using most fluorescence microscopes, as well as infrared imaging systems.[1][2]
To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.
DyLight Fluors are synthesized through sulfonate addition to coumarin, xanthene (such as fluorescein and rhodamine), and cyanine dyes. Sulfonation makes DyLight dyes negatively charged and hydrophilic. DyLight Fluors are commercially available as reactive succinimidyl-esters for labeling proteins through lysineresidues, and as maleimide derivatives for labeling proteins through cysteine residues. Antibodies conjugated are also available from several companies.
^ ab"DyLight Fluors - Technology and Product Guide". Pierce Protein Research Products. 2011. Archived from the original on 2013-10-17. Retrieved 2013-10-17.
^ ab"DyLight Reactive Dyes". Pierce Protein Research Products. 2008. Retrieved 2013-10-17.
^"DyLight Reactive Dyes". Pierce Protein Research Products. Retrieved 2014-09-10.
^"Fisher Biosciences Collaborates with Dyomics to Add Fluorescent Reagents for Protein Research". Press release. BNET. 2006-01-09. Retrieved 2008-12-09.
January 26, 2023
dylight, fluor, color, mass, absorb, emit, dylight, violet, 000dylight, violet, 000dylight, green, 1011, 000dylight, yellow, 000dylight, orange, 1078, 000dylight, 1066, 000dylight, 1008, 000dylight, 000dylight, near, 1092, 000dylight, near, 1050, 000reference,. Color mass g mol Absorb nm Emit nm e M 1cm 1 DyLight 350 violet 874 353 432 15 000DyLight 405 violet 793 400 420 30 000DyLight 488 green 1011 493 518 70 000DyLight 550 yellow 982 562 576 150 000DyLight 594 orange 1078 593 618 80 000DyLight 633 red 1066 638 658 170 000DyLight 650 red 1008 654 673 250 000DyLight 680 far red 950 692 712 140 000DyLight 755 near IR 1092 754 776 220 000DyLight 800 near IR 1050 777 794 270 000Reference 1 2 3 The DyLight Fluor family of fluorescent dyes are produced by Dyomics in collaboration with Thermo Fisher Scientific 4 DyLight dyes are typically used in biotechnology and research applications as biomolecule cell and tissue labels for fluorescence microscopy cell biology or molecular biology Historically fluorophores such as fluorescein rhodamine Cy3 and Cy5 have been used in a wide variety of applications These dyes have limitations for use in microscopy and other applications that require exposure to an intense light source such as a laser because they photobleach quickly however bleaching can be reduced at least 10 fold using oxygen scavenging DyLight Fluors have comparable excitation and emission spectra and are claimed to be more photostable brighter and less pH sensitive The excitation and emission spectra of the DyLight Fluor series cover much of the visible spectrum and extend into the infrared region allowing detection using most fluorescence microscopes as well as infrared imaging systems 1 2 To use the DyLight Fluors with fluorescent imagers use a spectral line of the blue laser diode for DyLight 405 a cyan 488 nm laser for DyLight 488 a green 526 nm laser for DyLight 550 and 594 and a red 633 nm laser for DyLight 633 and 650 The DyLight 680 755 and 800 fluors are compatible with laser and filter based infrared imaging instruments that emit in the 700 nm 750 nm and 800 nm region of the spectrum respectively DyLight Fluors are synthesized through sulfonate addition to coumarin xanthene such as fluorescein and rhodamine and cyanine dyes Sulfonation makes DyLight dyes negatively charged and hydrophilic DyLight Fluors are commercially available as reactive succinimidyl esters for labeling proteins through lysine residues and as maleimide derivatives for labeling proteins through cysteine residues Antibodies conjugated are also available from several companies Similar lines of fluorescent dyes provide an alternative to the DyLight Dyes see also the list in Category Fluorescent dyes References Edit a b DyLight Fluors Technology and Product Guide Pierce Protein Research Products 2011 Archived from the original on 2013 10 17 Retrieved 2013 10 17 a b DyLight Reactive Dyes Pierce Protein Research Products 2008 Retrieved 2013 10 17 DyLight Reactive Dyes Pierce Protein Research Products Retrieved 2014 09 10 Fisher Biosciences Collaborates with Dyomics to Add Fluorescent Reagents for Protein Research Press release BNET 2006 01 09 Retrieved 2008 12 09 Retrieved from https en wikipedia org w index php title DyLight Fluor amp oldid 1060770283, wikipedia, wiki, book, books, library,
