A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 Mnitric acid, which protonates the iminodiacetate groups.
Chelex resin is often used for DNA extraction in preparation for polymerase chain reaction by binding to cations including Mg2+, which is an essential cofactor for DNases. Chelex protects the sample from DNases that might remain active after the boiling and could subsequently degrade the DNA, rendering it unsuitable for PCR. After boiling, the Chelex-DNA preparation is stable and can be stored at 4°C for 3–4 months.[2] Polar resin beads bind polar cellular components after breaking open cells, while DNA and RNA remain in water solution above the Chelex resin.
However, the heating steps do denature the double helix, and the resulting single-stranded DNA is less stable in storage.
Referencesedit
^"Chelex® 100 and Chelex 20 Chelating Ion Exchange Resin Instruction Manual" (PDF).
^Walsh, P.S., Metzger D.A., and Higuchi, R. (1991). "Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material". BioTechniques. 10 (4): 506–513. PMID 1867860.{{cite journal}}: CS1 maint: multiple names: authors list (link)
Daniel Harris. Quantitative Chemical Analysis, seventh edition, 2007. ISBN0-7167-7041-5. Page 594.
R. N. Ceo; M. R. Kazerouni; K. Rengan (1993). "Sorption of silver ions by Chelex 100 chelating resin". Journal of Radioanalytical and Nuclear Chemistry. 172 (1): 43–48. doi:10.1007/BF02040660. S2CID 94639747.
External linksedit
Official site
January 01, 1970
chelex, chelating, material, from, used, purify, other, compounds, exchange, noteworthy, ability, bind, transition, metal, ions, styrene, divinylbenzene, polymer, containing, iminodiacetic, acid, groups, concentrated, solution, metals, obtained, eluting, resin. Chelex 100 is a chelating material from Bio Rad 1 used to purify other compounds via ion exchange It is noteworthy for its ability to bind transition metal ions It is a styrene divinylbenzene co polymer containing iminodiacetic acid groups A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 M nitric acid which protonates the iminodiacetate groups Chelex resin is often used for DNA extraction in preparation for polymerase chain reaction by binding to cations including Mg2 which is an essential cofactor for DNases Chelex protects the sample from DNases that might remain active after the boiling and could subsequently degrade the DNA rendering it unsuitable for PCR After boiling the Chelex DNA preparation is stable and can be stored at 4 C for 3 4 months 2 Polar resin beads bind polar cellular components after breaking open cells while DNA and RNA remain in water solution above the Chelex resin However the heating steps do denature the double helix and the resulting single stranded DNA is less stable in storage References edit Chelex 100 and Chelex 20 Chelating Ion Exchange Resin Instruction Manual PDF Walsh P S Metzger D A and Higuchi R 1991 Chelex 100 as a Medium for Simple Extraction of DNA for PCR Based Typing from Forensic Material BioTechniques 10 4 506 513 PMID 1867860 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint multiple names authors list link Daniel Harris Quantitative Chemical Analysis seventh edition 2007 ISBN 0 7167 7041 5 Page 594 R N Ceo M R Kazerouni K Rengan 1993 Sorption of silver ions by Chelex 100 chelating resin Journal of Radioanalytical and Nuclear Chemistry 172 1 43 48 doi 10 1007 BF02040660 S2CID 94639747 External links editOfficial site Retrieved from https en wikipedia org w index php title Chelex 100 amp oldid 1147041588, wikipedia, wiki, book, books, library,
