Bicinchoninic is an organic compound with the formula (C9H5NCO2H)2. The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group. Its sodium salt forms a purple complex with cuprous ions.[1][2][3]
Bicinchoninic acid is most commonly employed in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. Bicinchoninic acid is used to detect the presence of cuprous ions, due to its purple coloration via a biuret reaction. In this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[4]
^Brown, Rhoderick E.; Jarvis, Kari L.; Hyland, Kristi J. (1989). "Protein measurement using bicinchoninic acid: Elimination of interfering substances". Analytical Biochemistry. 180 (1): 136–139. doi:10.1016/0003-2697(89)90101-2. PMID 2817336.
^Noble, James E.; Bailey, Marc J.A. (2009). "Chapter 8 Quantitation of Protein". Guide to Protein Purification, 2nd Edition. Methods in Enzymology. Vol. 463. pp. 73–95. doi:10.1016/S0076-6879(09)63008-1. ISBN9780123745361. PMID 19892168.
bicinchoninic, acid, ɔː, weak, acid, composed, carboxylated, quinoline, rings, names, preferred, iupac, name, biquinoline, dicarboxylic, acid, other, names, dicarboxy, biquinoline2, biquinoline, dicarboxylic, acid, identifiers, number, 1245, free, acid, y979, . Bicinchoninic acid b aɪ s ɪ n k ɔː n ɪ n ɪ k is a weak acid composed of two carboxylated quinoline rings Bicinchoninic acid Names Preferred IUPAC name 2 2 Biquinoline 4 4 dicarboxylic acid Other names Bicinchoninic acid4 4 Dicarboxy 2 2 biquinoline2 2 Biquinoline 4 4 dicarboxylic acid Identifiers CAS Number 1245 13 2 free acid Y979 88 4 disodium salt N 3D model JSmol Interactive image ChEMBL ChEMBL431482 Y ChemSpider 64223 Y ECHA InfoCard 100 013 628 PubChem CID 71068 UNII CX56TX9Y1I Y CompTox Dashboard EPA DTXSID8061638 InChI InChI 1S C20H12N2O4 c23 19 24 13 9 17 21 15 7 3 1 5 11 13 15 18 10 14 20 25 26 12 6 2 4 8 16 12 22 18 h1 10H H 23 24 H 25 26 YKey AFYNADDZULBEJA UHFFFAOYSA N Y SMILES O C O c1cc nc2c1cccc2 c3nc4ccccc4c c3 C O O Properties Chemical formula C 20H 12N 2O 4 Molar mass 344 326 g mol 1 Appearance Cream colored powder Odor Characteristic odor vague Melting point 352 C 666 F 625 K decomposes Solubility in water Partially soluble in cold water hot water vague Hazards Occupational safety and health OHS OSH Main hazards Will irritate eyes and mucous membranes NFPA 704 fire diamond 1 Safety data sheet SDS External MSDS Except where otherwise noted data are given for materials in their standard state at 25 C 77 F 100 kPa Y verify what is Y N Infobox references Bicinchoninic is an organic compound with the formula C9H5NCO2H 2 The molecule consists of a pair of quinoline rings each bearing a carboxylic acid group Its sodium salt forms a purple complex with cuprous ions 1 2 3 Bicinchoninic acid is most commonly employed in the bicinchoninic acid BCA assay which is used to determine the total level of protein in a solution Bicinchoninic acid is used to detect the presence of cuprous ions due to its purple coloration via a biuret reaction In this assay two molecules of bicinchoninic acid chelate a single Cu ion forming a purple water soluble complex that strongly absorbs light at 562 nm 4 References edit Smith P K Krohn R I Hermanson G T Mallia A K Gartner F H Provenzano M D Fujimoto E K Goeke N M Olson B J Klenk D C 1985 Measurement of Protein Using Bicinchoninic Acid Analytical Biochemistry 150 1 76 85 doi 10 1016 0003 2697 85 90442 7 PMID 3843705 Brown Rhoderick E Jarvis Kari L Hyland Kristi J 1989 Protein measurement using bicinchoninic acid Elimination of interfering substances Analytical Biochemistry 180 1 136 139 doi 10 1016 0003 2697 89 90101 2 PMID 2817336 Noble James E Bailey Marc J A 2009 Chapter 8 Quantitation of Protein Guide to Protein Purification 2nd Edition Methods in Enzymology Vol 463 pp 73 95 doi 10 1016 S0076 6879 09 63008 1 ISBN 9780123745361 PMID 19892168 Pierce BCA Protein Assay Kit Retrieved from https en wikipedia org w index php title Bicinchoninic acid amp oldid 1184920123, wikipedia, wiki, book, books, library,
