4-Fluoro-7-nitrobenzofurazan (NBD-F) is a fluorogenic, amine labeling dye that is not fluorescent itself, but covalently reacts with secondary or primary amines to form a fluorescently labeled product.[2] It and other fluorogenic benzofurans (i.e. NBD-Cl) are used for derivitization in HPLC applications.[3] After the fluorogenic reaction, it can be detected with an excitation wavelength of 470 nm (blue) and an emission wavelength of 530 nm (green), enabling an HPLC limit of detection of 10 fmol.[4]
^"4-Fluoro-7-nitrobenzofurazan". PubChem. National Library of Medicine. Retrieved 11 March 2023.
^Stoyanov, Alexander V.; Ahmadzadeh, Hossein; Krylov, Sergey N. (2002-11-25). "Heterogeneity of protein labeling with a fluorogenic reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde". Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 780 (2): 283–287. doi:10.1016/s1570-0232(02)00535-4. ISSN 1570-0232. PMID 12401353.
^Maroulis, Marios; Monemvasios, Ioannis; Vardaka, Elisabeth; Rigas, Pantelis (2008). "Determination of domoic acid in mussels by HPLC with post-column derivatization using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and fluorescence detection". Journal of Chromatography B. Elsevier BV. 876 (2): 245–251. doi:10.1016/j.jchromb.2008.10.053. ISSN 1570-0232. PMID 19026599.
^Watanabe, Yoshihiko; Imai, Kazuhiro (1981). "High-performance liquid chromatography and sensitive detection of amino acids derivatized with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole". Analytical Biochemistry. Elsevier BV. 116 (2): 471–472. doi:10.1016/0003-2697(81)90390-0. ISSN 0003-2697. PMID 7316179.
This article about an organic compound is a stub. You can help Wikipedia by expanding it.
fluoro, nitrobenzofurazan, fluorogenic, amine, labeling, that, fluorescent, itself, covalently, reacts, with, secondary, primary, amines, form, fluorescently, labeled, product, other, fluorogenic, benzofurans, used, derivitization, hplc, applications, after, f. 4 Fluoro 7 nitrobenzofurazan NBD F is a fluorogenic amine labeling dye that is not fluorescent itself but covalently reacts with secondary or primary amines to form a fluorescently labeled product 2 It and other fluorogenic benzofurans i e NBD Cl are used for derivitization in HPLC applications 3 After the fluorogenic reaction it can be detected with an excitation wavelength of 470 nm blue and an emission wavelength of 530 nm green enabling an HPLC limit of detection of 10 fmol 4 NBD F 1 NamesIUPAC name 4 fluoro 7 nitro 2 1 3 benzoxadiazoleOther names NBD FIdentifiersCAS Number 29270 56 2 Y3D model JSmol Interactive imageChemSpider 108923ECHA InfoCard 100 162 766EC Number 634 820 0PubChem CID 122123CompTox Dashboard EPA DTXSID20183521InChI InChI 1S C6H2FN3O3 c7 3 1 2 4 10 11 12 6 5 3 8 13 9 6 h1 2HKey PGZIDERTDJHJFY UHFFFAOYSA NSMILES C1 C C2 NON C2C C1 F N O O PropertiesChemical formula C6H2FN3O3Molar mass 183 10 g molExcept where otherwise noted data are given for materials in their standard state at 25 C 77 F 100 kPa Infobox referencesSee also EditFluorescamine FQReferences Edit 4 Fluoro 7 nitrobenzofurazan PubChem National Library of Medicine Retrieved 11 March 2023 Stoyanov Alexander V Ahmadzadeh Hossein Krylov Sergey N 2002 11 25 Heterogeneity of protein labeling with a fluorogenic reagent 3 2 furoyl quinoline 2 carboxaldehyde Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences 780 2 283 287 doi 10 1016 s1570 0232 02 00535 4 ISSN 1570 0232 PMID 12401353 Maroulis Marios Monemvasios Ioannis Vardaka Elisabeth Rigas Pantelis 2008 Determination of domoic acid in mussels by HPLC with post column derivatization using 4 chloro 7 nitrobenzo 2 oxa 1 3 diazole NBD Cl and fluorescence detection Journal of Chromatography B Elsevier BV 876 2 245 251 doi 10 1016 j jchromb 2008 10 053 ISSN 1570 0232 PMID 19026599 Watanabe Yoshihiko Imai Kazuhiro 1981 High performance liquid chromatography and sensitive detection of amino acids derivatized with 7 fluoro 4 nitrobenzo 2 oxa 1 3 diazole Analytical Biochemistry Elsevier BV 116 2 471 472 doi 10 1016 0003 2697 81 90390 0 ISSN 0003 2697 PMID 7316179 nbsp This article about an organic compound is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title 4 Fluoro 7 nitrobenzofurazan amp oldid 1144519411, wikipedia, wiki, book, books, library,
