"Amplification" means creating many more copies of a strand of nucleic acid than was present at first, in order to readily detect it or test it. Rapidly amplifying the target RNA/DNA allows a lab to simultaneously detect multiple pathogenic organisms in a single tube. TMA technology allows a clinical laboratory to perform nucleic acid test (NAT) assays for blood screening with fewer steps, less processing time, and faster results. It is used in molecular biology, forensics, and medicine for the rapid identification and diagnosis of pathogenic organisms. In contrast to similar techniques such as polymerase chain reaction and ligase chain reaction, this method involves RNA transcription (via RNA polymerase) and DNA synthesis (via reverse transcriptase) to produce an RNA amplicon (the source or product of amplification) from a target nucleic acid. This technique can be used to target both RNA and DNA.
Transcription-mediated amplification has several advantages compared to other amplification methods including:
TMA is isothermal; a water bath or heat block is used instead of a thermal cycler.
TMA produces RNA amplicon rather than DNA amplicon. Since RNA is more labile in a laboratory environment, this reduces the possibility of carry-over contamination.
TMA produces 100–1000 copies per cycle (PCR and LCR exponentially doubles each cycle). This results in a 10 billion fold increase of DNA (or RNA) copies within about 15–30 minutes.
RNA -> DNA -> RNA
Referencesedit
Daniel L. Kacian, Timothy J. Fultz: Nucleic acid sequence amplification methods. In: Biotechnology Advances 1995, 13.3, S. 569–569
transcription, mediated, amplification, this, article, technical, most, readers, understand, please, help, improve, make, understandable, experts, without, removing, technical, details, 2014, learn, when, remove, this, message, isothermal, performed, constant,. This article may be too technical for most readers to understand Please help improve it to make it understandable to non experts without removing the technical details May 2014 Learn how and when to remove this message Transcription mediated amplification TMA is an isothermal performed at constant temperature single tube nucleic acid amplification system utilizing two enzymes RNA polymerase and reverse transcriptase Amplification means creating many more copies of a strand of nucleic acid than was present at first in order to readily detect it or test it Rapidly amplifying the target RNA DNA allows a lab to simultaneously detect multiple pathogenic organisms in a single tube TMA technology allows a clinical laboratory to perform nucleic acid test NAT assays for blood screening with fewer steps less processing time and faster results It is used in molecular biology forensics and medicine for the rapid identification and diagnosis of pathogenic organisms In contrast to similar techniques such as polymerase chain reaction and ligase chain reaction this method involves RNA transcription via RNA polymerase and DNA synthesis via reverse transcriptase to produce an RNA amplicon the source or product of amplification from a target nucleic acid This technique can be used to target both RNA and DNA Transcription mediated amplification has several advantages compared to other amplification methods including TMA is isothermal a water bath or heat block is used instead of a thermal cycler TMA produces RNA amplicon rather than DNA amplicon Since RNA is more labile in a laboratory environment this reduces the possibility of carry over contamination TMA produces 100 1000 copies per cycle PCR and LCR exponentially doubles each cycle This results in a 10 billion fold increase of DNA or RNA copies within about 15 30 minutes RNA gt DNA gt RNAReferences editDaniel L Kacian Timothy J Fultz Nucleic acid sequence amplification methods In Biotechnology Advances 1995 13 3 S 569 569 From http www gen probe com nbsp This biochemistry article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Transcription mediated amplification amp oldid 1136552322, wikipedia, wiki, book, books, library,
