Sp100 nuclear antigen is an interferon stimulated antigen found in the cell nuclei of many human and higher animal cells. Autoantibodies directed against Sp100 are often found in patients with primary biliary cirrhosis.[1] Histologically Sp100 'dots' regions of the cell nucleus. Viral infection and mitogens affect the expression of the Sp100 autoantigen. Cells grown in the presence of interferons (α, β, and γ) revealed an increase both in size and number of the Sp100 protein-containing nuclear dots and increase the protein concentration. This raises "the question whether cytokine-mediated increase of Sp100 protein expression plays a role in induction of anti-Sp100 autoantibodies."[2]
Immunofluorescence staining pattern of anti-Sp100 antibodies on HEp-20-10 cells.
Two proteins, Sp100 and promyelocytic leukemia (PML) factor are localized to punctate domains in the nucleus (nuclear dots or nuclear bodies). These domains (few to 20) were found to form a donut-shaped structure when cells were starved of amino acids. In particular, depravation of cystine results in most pronounced changes.[3] Two other proteins, PIC1/SUMO-1, that also interact with nuclear pore complex factors also interact with these two proteins.[4] In addition Sp100 interacts with a chromatin binding protein, HP1 alpha.[5]
Sp100 splicoformsedit
Some Sp100 variants contain a domain similar to two interferon-inducible nuclear phosphoproteins, suppressin and DEAF1. This defines a novel protein motif, the HNPP-box. Another class of variants has high mobility group 1 (HMG1) protein sequence as a domain. Both major classes of Sp100 splice variant proteins localize in part to nuclear dots/PML bodies and other nuclear domains.[6]
Referencesedit
^Szostecki C, Guldner HH, Netter HJ, Will H (1990). "Isolation and characterization of cDNA encoding a human nuclear antigen predominantly recognized by autoantibodies from patients with primary biliary cirrhosis". J. Immunol. 145 (12): 4338–47. doi:10.4049/jimmunol.145.12.4338. PMID 2258622. S2CID 43572051.
^Guldner HH, Szostecki C, Grötzinger T, Will H (1992). "IFN enhance expression of Sp100, an autoantigen in primary biliary cirrhosis". J. Immunol. 149 (12): 4067–73. doi:10.4049/jimmunol.149.12.4067. PMID 1281200. S2CID 20921655.
^Kamei H (1997). "Cystine starvation induces reversible large-body formation from nuclear bodies in T24 cells". Exp. Cell Res. 237 (1): 207–16. doi:10.1006/excr.1997.3790. PMID 9417884.
^Sternsdorf T, Jensen K, Will H (1997). "Evidence for covalent modification of the nuclear dot-associated proteins PML and Sp100 by PIC1/SUMO-1". J. Cell Biol. 139 (7): 1621–34. doi:10.1083/jcb.139.7.1621. PMC2132645. PMID 9412458.
^Seeler JS, Marchio A, Sitterlin D, Transy C, Dejean A (1998). "Interaction of SP100 with HP1 proteins: a link between the promyelocytic leukemia-associated nuclear bodies and the chromatin compartment". Proc. Natl. Acad. Sci. U.S.A. 95 (13): 7316–21. Bibcode:1998PNAS...95.7316S. doi:10.1073/pnas.95.13.7316. PMC22602. PMID 9636146.
^Guldner HH, Szostecki C, Schröder P, et al. (1999). "Splice variants of the nuclear dot-associated Sp100 protein contain homologies to HMG-1 and a human nuclear phosphoprotein-box motif". J. Cell Sci. 112. ( Pt 5) (5): 733–47. doi:10.1242/jcs.112.5.733. PMID 9973607.
April 10, 2024
sp100, nuclear, antigen, interferon, stimulated, antigen, found, cell, nuclei, many, human, higher, animal, cells, autoantibodies, directed, against, sp100, often, found, patients, with, primary, biliary, cirrhosis, histologically, sp100, dots, regions, cell, . Sp100 nuclear antigen is an interferon stimulated antigen found in the cell nuclei of many human and higher animal cells Autoantibodies directed against Sp100 are often found in patients with primary biliary cirrhosis 1 Histologically Sp100 dots regions of the cell nucleus Viral infection and mitogens affect the expression of the Sp100 autoantigen Cells grown in the presence of interferons a b and g revealed an increase both in size and number of the Sp100 protein containing nuclear dots and increase the protein concentration This raises the question whether cytokine mediated increase of Sp100 protein expression plays a role in induction of anti Sp100 autoantibodies 2 SP100 nuclear antigenIdentifiersSymbolSP100NCBI gene6672HGNC11206OMIM604585RefSeqNM 003113UniProtP23497Other dataLocusChr 2 q37 1Search forStructuresSwiss modelDomainsInterProSp100 and nuclear dots edit nbsp Immunofluorescence staining pattern of anti Sp100 antibodies on HEp 20 10 cells Two proteins Sp100 and promyelocytic leukemia PML factor are localized to punctate domains in the nucleus nuclear dots or nuclear bodies These domains few to 20 were found to form a donut shaped structure when cells were starved of amino acids In particular depravation of cystine results in most pronounced changes 3 Two other proteins PIC1 SUMO 1 that also interact with nuclear pore complex factors also interact with these two proteins 4 In addition Sp100 interacts with a chromatin binding protein HP1 alpha 5 Sp100 splicoforms editSome Sp100 variants contain a domain similar to two interferon inducible nuclear phosphoproteins suppressin and DEAF1 This defines a novel protein motif the HNPP box Another class of variants has high mobility group 1 HMG1 protein sequence as a domain Both major classes of Sp100 splice variant proteins localize in part to nuclear dots PML bodies and other nuclear domains 6 References edit Szostecki C Guldner HH Netter HJ Will H 1990 Isolation and characterization of cDNA encoding a human nuclear antigen predominantly recognized by autoantibodies from patients with primary biliary cirrhosis J Immunol 145 12 4338 47 doi 10 4049 jimmunol 145 12 4338 PMID 2258622 S2CID 43572051 Guldner HH Szostecki C Grotzinger T Will H 1992 IFN enhance expression of Sp100 an autoantigen in primary biliary cirrhosis J Immunol 149 12 4067 73 doi 10 4049 jimmunol 149 12 4067 PMID 1281200 S2CID 20921655 Kamei H 1997 Cystine starvation induces reversible large body formation from nuclear bodies in T24 cells Exp Cell Res 237 1 207 16 doi 10 1006 excr 1997 3790 PMID 9417884 Sternsdorf T Jensen K Will H 1997 Evidence for covalent modification of the nuclear dot associated proteins PML and Sp100 by PIC1 SUMO 1 J Cell Biol 139 7 1621 34 doi 10 1083 jcb 139 7 1621 PMC 2132645 PMID 9412458 Seeler JS Marchio A Sitterlin D Transy C Dejean A 1998 Interaction of SP100 with HP1 proteins a link between the promyelocytic leukemia associated nuclear bodies and the chromatin compartment Proc Natl Acad Sci U S A 95 13 7316 21 Bibcode 1998PNAS 95 7316S doi 10 1073 pnas 95 13 7316 PMC 22602 PMID 9636146 Guldner HH Szostecki C Schroder P et al 1999 Splice variants of the nuclear dot associated Sp100 protein contain homologies to HMG 1 and a human nuclear phosphoprotein box motif J Cell Sci 112 Pt 5 5 733 47 doi 10 1242 jcs 112 5 733 PMID 9973607 Retrieved from https en wikipedia org w index php title Sp100 nuclear antigen amp oldid 1197459376, wikipedia, wiki, book, books, library,
