The SMKbox riboswitch (also known as SAM-III) is an RNA element that regulates gene expression in bacteria.[2][3] The SMK box riboswitch is found in the 5' UTR of the MetK gene in lactic acid bacteria. The structure of this element changes upon binding to S-adenosyl methionine (SAM) to a conformation that blocks the shine-dalgarno sequence and blocks translation of the gene.
SMK box translational riboswitch
A representation of the SMK box translational riboswitch secondary structure including a colour scheme that indicates the degree of sequence conservation.
The crystal structure of the riboswitch from E. faecalis was solved by X-ray crystallography. The structure showed that the most conserved nucleotides involved in SAM binding were organised around a junction between three helices.[1] In some species there are large insertions of up to 210 nucleotides within this structure.
^ abLu C, Smith AM, Fuchs RT, Ding F, Rajashankar K, Henkin TM, Ke A (2008). "Crystal structures of the SAM-III/S(MK) riboswitch reveal the SAM-dependent translation inhibition mechanism". Nat Struct Mol Biol. 15 (10): 1076–1083. doi:10.1038/nsmb.1494. PMC3467307. PMID 18806797.
^Fuchs RT, Grundy FJ, Henkin TM (2006). "The S(MK) box is a new SAM-binding RNA for translational regulation of SAM synthetase". Nat. Struct. Mol. Biol. 13 (3): 226–233. doi:10.1038/nsmb1059. PMID 16491091. S2CID 8481958.
^Fuchs RT, Grundy FJ, Henkin TM (2007). "S-adenosylmethionine directly inhibits binding of 30S ribosomal subunits to the SMK box translational riboswitch RNA". Proc. Natl. Acad. Sci. U.S.A. 104 (12): 4876–4880. Bibcode:2007PNAS..104.4876F. doi:10.1073/pnas.0609956104. PMC1829232. PMID 17360376.
riboswitch, smkbox, riboswitch, also, known, element, that, regulates, gene, expression, bacteria, found, metk, gene, lactic, acid, bacteria, structure, this, element, changes, upon, binding, adenosyl, methionine, conformation, that, blocks, shine, dalgarno, s. The SMKbox riboswitch also known as SAM III is an RNA element that regulates gene expression in bacteria 2 3 The SMK box riboswitch is found in the 5 UTR of the MetK gene in lactic acid bacteria The structure of this element changes upon binding to S adenosyl methionine SAM to a conformation that blocks the shine dalgarno sequence and blocks translation of the gene SMK box translational riboswitchA representation of the SMK box translational riboswitch secondary structure including a colour scheme that indicates the degree of sequence conservation IdentifiersSymbolSMK box riboswitchAlt SymbolsSAM III SMKRfamRF01767Other dataRNA typeRiboswitchDomain s BacillotaPDB structuresPDBe 3e5f 3e5e 3e5cA 3D representation of the SMKbox riboswitch structure 1 There are other known SAM binding riboswitches such as SAM I and SAM II but these appear to share no similarity in sequence or structure to SAM III Contents 1 Structure 2 See also 3 References 4 External linksStructure editThe crystal structure of the riboswitch from E faecalis was solved by X ray crystallography The structure showed that the most conserved nucleotides involved in SAM binding were organised around a junction between three helices 1 In some species there are large insertions of up to 210 nucleotides within this structure See also editSAH riboswitch SAM I riboswitch SAM II riboswitch SAM IV riboswitch SAM V riboswitch SAM VI riboswitch SAM Chlorobi RNA motif SAM SAH riboswitchReferences edit a b Lu C Smith AM Fuchs RT Ding F Rajashankar K Henkin TM Ke A 2008 Crystal structures of the SAM III S MK riboswitch reveal the SAM dependent translation inhibition mechanism Nat Struct Mol Biol 15 10 1076 1083 doi 10 1038 nsmb 1494 PMC 3467307 PMID 18806797 Fuchs RT Grundy FJ Henkin TM 2006 The S MK box is a new SAM binding RNA for translational regulation of SAM synthetase Nat Struct Mol Biol 13 3 226 233 doi 10 1038 nsmb1059 PMID 16491091 S2CID 8481958 Fuchs RT Grundy FJ Henkin TM 2007 S adenosylmethionine directly inhibits binding of 30S ribosomal subunits to the SMK box translational riboswitch RNA Proc Natl Acad Sci U S A 104 12 4876 4880 Bibcode 2007PNAS 104 4876F doi 10 1073 pnas 0609956104 PMC 1829232 PMID 17360376 External links editPage for SMK box translational riboswitch at Rfam nbsp This molecular or cell biology article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title SMK box riboswitch amp oldid 1130022146, wikipedia, wiki, book, books, library,
