RevCen is a family of non-coding RNA found in Schizosaccharomyces. It is a megastructure containing several siRNA which use the RNAi pathway to regulate heterochromatin formation. The long RNA transcript forms a secondary structure with several stem-loops which are processed by dicer into siRNA. This siRNA then initiate the formation of heterochromatin at the centromeres of fission yeast.[1]Northern blot analysis confirmed the siRNAs were produced from the large RNA structure RevCen in vivo. As with all siRNAs, the enzyme dicer is responsible for dissecting dsRNA into the 21nt stretch of double-stranded RNA. Human recombinant dicer enzyme processed the RevCen structure in vitro, though the same activity by yeast Dcr1 has not been confirmed.[1]
This is a different mechanism to that involving the well-understood RITS (RNA-induced initiation of transcriptional gene silencing) complex.[2][3] It has been suggested that both mechanisms work together, with the RevCen structure potentially acting as a backup system when Rdp1-generated double-stranded siRNA precursors are lost.[1]
Referencesedit
^ abcDjupedal I, Kos-Braun IC, Mosher RA, et al. (December 2009). "Analysis of small RNA in fission yeast; centromeric siRNAs are potentially generated through a structured RNA". EMBO J. 28 (24): 3832–3844. doi:10.1038/emboj.2009.351. PMC2797062. PMID 19942857.
^Sugiyama T, Cam H, Verdel A, Moazed D, Grewal SI (January 2005). "RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production". Proc. Natl. Acad. Sci. U.S.A. 102 (1): 152–157. doi:10.1073/pnas.0407641102. PMC544066. PMID 15615848.
^Verdel A, Jia S, Gerber S, et al. (January 2004). "RNAi-mediated targeting of heterochromatin by the RITS complex". Science. 303 (5658): 672–676. doi:10.1126/science.1093686. PMC3244756. PMID 14704433.
Further readingedit
Vavasseur; et al. (2008). "Heterochromatin Assembly and Transcriptional Gene Silencing under the Control of Nuclear RNAi: Lessons from Fission Yeast". RNA and the Regulation of Gene Expression: A Hidden Layer of Complexity. Norfolk: Caister Academic Press. ISBN978-1-904455-25-7.
Sigova A, Rhind N, Zamore PD (October 2004). "A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe". Genes Dev. 18 (19): 2359–2367. doi:10.1101/gad.1218004. PMC522986. PMID 15371329. Retrieved 2010-08-17.
Steiner NC, Hahnenberger KM, Clarke L (August 1993). "Centromeres of the fission yeast Schizosaccharomyces pombe are highly variable genetic loci". Mol. Cell. Biol. 13 (8): 4578–4587. doi:10.1128/MCB.13.8.4578. PMC360078. PMID 8336703.
Takahashi K, Murakami S, Chikashige Y, Niwa O, Yanagida M (March 1991). "A large number of tRNA genes are symmetrically located in fission yeast centromeres". J. Mol. Biol. 218 (1): 13–17. doi:10.1016/0022-2836(91)90867-6. PMID 2002498.
January 01, 1970
revcen, family, coding, found, schizosaccharomyces, megastructure, containing, several, sirna, which, rnai, pathway, regulate, heterochromatin, formation, long, transcript, forms, secondary, structure, with, several, stem, loops, which, processed, dicer, into,. RevCen is a family of non coding RNA found in Schizosaccharomyces It is a megastructure containing several siRNA which use the RNAi pathway to regulate heterochromatin formation The long RNA transcript forms a secondary structure with several stem loops which are processed by dicer into siRNA This siRNA then initiate the formation of heterochromatin at the centromeres of fission yeast 1 Northern blot analysis confirmed the siRNAs were produced from the large RNA structure RevCen in vivo As with all siRNAs the enzyme dicer is responsible for dissecting dsRNA into the 21nt stretch of double stranded RNA Human recombinant dicer enzyme processed the RevCen structure in vitro though the same activity by yeast Dcr1 has not been confirmed 1 RevCenConserved secondary structure of RevCen RNA IdentifiersSymbolRevCenOther dataRNA typeGene pre miRNADomain s SchizosaccharomycesPDB structuresPDBe This is a different mechanism to that involving the well understood RITS RNA induced initiation of transcriptional gene silencing complex 2 3 It has been suggested that both mechanisms work together with the RevCen structure potentially acting as a backup system when Rdp1 generated double stranded siRNA precursors are lost 1 References edit a b c Djupedal I Kos Braun IC Mosher RA et al December 2009 Analysis of small RNA in fission yeast centromeric siRNAs are potentially generated through a structured RNA EMBO J 28 24 3832 3844 doi 10 1038 emboj 2009 351 PMC 2797062 PMID 19942857 Sugiyama T Cam H Verdel A Moazed D Grewal SI January 2005 RNA dependent RNA polymerase is an essential component of a self enforcing loop coupling heterochromatin assembly to siRNA production Proc Natl Acad Sci U S A 102 1 152 157 doi 10 1073 pnas 0407641102 PMC 544066 PMID 15615848 Verdel A Jia S Gerber S et al January 2004 RNAi mediated targeting of heterochromatin by the RITS complex Science 303 5658 672 676 doi 10 1126 science 1093686 PMC 3244756 PMID 14704433 Further reading editVavasseur et al 2008 Heterochromatin Assembly and Transcriptional Gene Silencing under the Control of Nuclear RNAi Lessons from Fission Yeast RNA and the Regulation of Gene Expression A Hidden Layer of Complexity Norfolk Caister Academic Press ISBN 978 1 904455 25 7 Sigova A Rhind N Zamore PD October 2004 A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe Genes Dev 18 19 2359 2367 doi 10 1101 gad 1218004 PMC 522986 PMID 15371329 Retrieved 2010 08 17 Steiner NC Hahnenberger KM Clarke L August 1993 Centromeres of the fission yeast Schizosaccharomyces pombe are highly variable genetic loci Mol Cell Biol 13 8 4578 4587 doi 10 1128 MCB 13 8 4578 PMC 360078 PMID 8336703 Takahashi K Murakami S Chikashige Y Niwa O Yanagida M March 1991 A large number of tRNA genes are symmetrically located in fission yeast centromeres J Mol Biol 218 1 13 17 doi 10 1016 0022 2836 91 90867 6 PMID 2002498 Retrieved from https en wikipedia org w index php title RevCen amp oldid 1044690405, wikipedia, wiki, book, books, library,
