SELPLG codes for PSGL-1, the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin.
The organization of the SELPLG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.[5]
P-selectin glycoprotein ligand-1 (PSGL-1) is a glycoprotein found on white blood cells and endothelial cells that binds to P-selectin (P stands for platelet), which is one of a family of selectins that includes E-selectin (endothelial) and L-selectin (leukocyte). Selectins are part of the broader family of cell adhesion molecules. PSGL-1 can bind to all three members of the family but binds best (with the highest affinity) to P-selectin.
PSGL-1 is expressed on all white blood cells and plays an important role in the recruitment of white blood cells into inflamed tissue: White blood cells normally do not interact with the endothelium of blood vessels. However, inflammation causes the expression of cell adhesion molecules (CAM) such as P-selectin on the surface of the blood vessel wall. White blood cells present in flowing blood can interact with CAM. The first step in this interaction process is carried out by PSGL-1 interacting with P-selectin and/or E-selectin on endothelial cells and adherent platelets. This interaction results in "rolling" of the white blood cell on the endothelial cell surface followed by stable adhesion and transmigration of the white blood cell into the inflamed tissue.[citation needed]
PSGL-1 has been shown to bind to VISTA (V-domain Ig suppressor of T cell activation) but this binding only occurs under acidic pH conditions (pH < 6.5) such as can be found in tumor microenvironments (TME). [11]
^Li F, Wilkins PP, Crawley S, et al. (1996). "Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin". J. Biol. Chem. 271 (6): 3255–64. doi:10.1074/jbc.271.6.3255. PMID 8621728.
^Wilkins PP, Moore KL, McEver RP, Cummings RD (1995). "Tyrosine sulfation of P-selectin glycoprotein ligand-1 is required for high affinity binding to P-selectin". J. Biol. Chem. 270 (39): 22677–80. doi:10.1074/jbc.270.39.22677. PMID 7559387.
^Sako D, Comess KM, Barone KM, et al. (1995). "A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding". Cell. 83 (2): 323–31. doi:10.1016/0092-8674(95)90173-6. PMID 7585949. S2CID 65420.
^Pouyani T, Seed B (1995). "PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus". Cell. 83 (2): 333–43. doi:10.1016/0092-8674(95)90174-4. PMID 7585950. S2CID 17480260.
^Immune Factor Seen to Control T-Cell Checkpoints Involved in Spread of Cancers and Infections. June 2016
^Reviving exhausted T cells to tackle immunotherapy-resistant cancers 2023
Further readingedit
Furie B, Furie BC (2004). "Role of platelet P-selectin and microparticle PSGL-1 in thrombus formation". Trends in Molecular Medicine. 10 (4): 171–8. doi:10.1016/j.molmed.2004.02.008. PMID 15059608.
Sako D, Chang XJ, Barone KM, et al. (1993). "Expression cloning of a functional glycoprotein ligand for P-selectin". Cell. 75 (6): 1179–86. doi:10.1016/0092-8674(93)90327-M. PMID 7505206. S2CID 23786141.
Moore KL, Eaton SF, Lyons DE, et al. (1994). "The P-selectin glycoprotein ligand from human neutrophils displays sialylated, fucosylated, O-linked poly-N-acetyllactosamine". J. Biol. Chem. 269 (37): 23318–27. doi:10.1016/S0021-9258(17)31656-3. PMID 7521878.
Veldman GM, Bean KM, Cumming DA, et al. (1995). "Genomic organization and chromosomal localization of the gene encoding human P-selectin glycoprotein ligand". J. Biol. Chem. 270 (27): 16470–5. doi:10.1074/jbc.270.27.16470. PMID 7541799.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Guyer DA, Moore KL, Lynam EB, et al. (1996). "P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation". Blood. 88 (7): 2415–21. doi:10.1182/blood.V88.7.2415.bloodjournal8872415. PMID 8839831.
Goetz DJ, Greif DM, Ding H, et al. (1997). "Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin". J. Cell Biol. 137 (2): 509–19. doi:10.1083/jcb.137.2.509. PMC2139768. PMID 9128259.
Fuhlbrigge RC, Kieffer JD, Armerding D, Kupper TS (1997). "Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells". Nature. 389 (6654): 978–81. Bibcode:1997Natur.389..978F. doi:10.1038/40166. PMID 9353122. S2CID 4376296.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Snapp KR, Ding H, Atkins K, et al. (1998). "A novel P-selectin glycoprotein ligand-1 monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL-1 and blocks recognition of both P- and L-selectin". Blood. 91 (1): 154–64. doi:10.1182/blood.V91.1.154. PMID 9414280.
Bennett EP, Hassan H, Mandel U, et al. (1998). "Cloning of a human UDP-N-acetyl-alpha-D-Galactosamine:polypeptide N-acetylgalactosaminyltransferase that complements other GalNAc-transferases in complete O-glycosylation of the MUC1 tandem repeat". J. Biol. Chem. 273 (46): 30472–81. doi:10.1074/jbc.273.46.30472. PMID 9804815.
Wimazal F, Ghannadan M, Müller MR, et al. (2000). "Expression of homing receptors and related molecules on human mast cells and basophils: a comparative analysis using multi-color flow cytometry and toluidine blue/immunofluorescence staining techniques". Tissue Antigens. 54 (5): 499–507. doi:10.1034/j.1399-0039.1999.540507.x. PMID 10599889.
Epperson TK, Patel KD, McEver RP, Cummings RD (2000). "Noncovalent association of P-selectin glycoprotein ligand-1 and minimal determinants for binding to P-selectin". J. Biol. Chem. 275 (11): 7839–53. doi:10.1074/jbc.275.11.7839. PMID 10713099.
André P, Spertini O, Guia S, et al. (2000). "Modification of P-selectin glycoprotein ligand-1 with a natural killer cell-restricted sulfated lactosamine creates an alternate ligand for L-selectin". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3400–5. doi:10.1073/pnas.040569797. PMC16251. PMID 10725346.
Frenette PS, Denis CV, Weiss L, et al. (2000). "P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo". J. Exp. Med. 191 (8): 1413–22. doi:10.1084/jem.191.8.1413. PMC2193129. PMID 10770806.
Woltmann G, McNulty CA, Dewson G, et al. (2000). "Interleukin-13 induces PSGL-1/P-selectin-dependent adhesion of eosinophils, but not neutrophils, to human umbilical vein endothelial cells under flow". Blood. 95 (10): 3146–52. doi:10.1182/blood.V95.10.3146. PMID 10807781.
Tsuchihashi S, Fondevila C, Shaw GD, et al. (2006). "Molecular characterization of rat leukocyte P-selectin glycoprotein ligand-1 and effect of its blockade: protection from ischemia-reperfusion injury in liver transplantation". J Immunol. 176 (1): 616–24. doi:10.4049/jimmunol.176.1.616. PMID 16365457.
External linksedit
P-selectin+glycoprotein+ligand-1 at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
selectin, glycoprotein, ligand, selectin, ligand, also, known, selplg, cd162, cluster, differentiation, human, gene, selplgavailable, structurespdbortholog, search, pdbe, rcsblist, codes1g1sidentifiersaliasesselplg, cd162, psgl, psgl1, selectin, ligandexternal. Selectin P ligand also known as SELPLG or CD162 cluster of differentiation 162 is a human gene SELPLGAvailable structuresPDBOrtholog search PDBe RCSBList of PDB id codes1G1SIdentifiersAliasesSELPLG CD162 CLA PSGL 1 PSGL1 selectin P ligandExternal IDsOMIM 600738 MGI 106689 HomoloGene 2261 GeneCards SELPLGGene location Human Chr Chromosome 12 human 1 Band12q24 11Start108 621 895 bp 1 End108 633 894 bp 1 Gene location Mouse Chr Chromosome 5 mouse 2 Band5 F 5 55 59 cMStart113 956 597 bp 2 End113 970 705 bp 2 RNA expression patternBgeeHumanMouse ortholog Top expressed inbloodmonocytespleenlymph nodeinferior ganglion of vagus nerveperiodontal fiberappendixbone marrow cellsvena cavaright lungTop expressed inthymusbone marrowspleenbloodankle jointentorhinal cortexsuperior frontal gyrussubcutaneous adipose tissueright lung lobesubstantia nigraMore reference expression dataBioGPSMore reference expression dataGene ontologyMolecular functionsignaling receptor binding protein binding virus receptor activityCellular componenturopod integral component of plasma membrane integral component of membrane membrane plasma membrane plasma membrane raftBiological processcellular response to interleukin 6 leukocyte adhesive activation viral entry into host cell viral process cell adhesion leukocyte migration leukocyte tethering or rollingSources Amigo QuickGOOrthologsSpeciesHumanMouseEntrez640420345EnsemblENSG00000110876ENSMUSG00000048163UniProtQ14242Q62170RefSeq mRNA NM 001206609NM 003006NM 009151RefSeq protein NP 001193538NP 002997n aLocation UCSC Chr 12 108 62 108 63 MbChr 5 113 96 113 97 MbPubMed search 3 4 WikidataView Edit HumanView Edit MouseSELPLG codes for PSGL 1 the high affinity counter receptor for P selectin on myeloid cells and stimulated T lymphocytes As such it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P selectin The organization of the SELPLG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb alpha both of which have an intron in the 5 prime noncoding region a long second exon containing the complete coding region and TATA less promoters 5 P selectin glycoprotein ligand 1 PSGL 1 is a glycoprotein found on white blood cells and endothelial cells that binds to P selectin P stands for platelet which is one of a family of selectins that includes E selectin endothelial and L selectin leukocyte Selectins are part of the broader family of cell adhesion molecules PSGL 1 can bind to all three members of the family but binds best with the highest affinity to P selectin Contents 1 Posttranslational modification 2 Function 2 1 In cancer 3 References 4 Further reading 5 External linksPosttranslational modification editPSGL 1 protein requires two distinct posttranslational modifications to gain its selectin binding activity 6 7 8 9 sulfation of tyrosines the addition of the sialyl Lewis x tetrasaccharide sLex to its O linked glycansFunction editPSGL 1 is expressed on all white blood cells and plays an important role in the recruitment of white blood cells into inflamed tissue White blood cells normally do not interact with the endothelium of blood vessels However inflammation causes the expression of cell adhesion molecules CAM such as P selectin on the surface of the blood vessel wall White blood cells present in flowing blood can interact with CAM The first step in this interaction process is carried out by PSGL 1 interacting with P selectin and or E selectin on endothelial cells and adherent platelets This interaction results in rolling of the white blood cell on the endothelial cell surface followed by stable adhesion and transmigration of the white blood cell into the inflamed tissue citation needed In cancer edit In mice it seems to be an immune factor regulating T cell checkpoints and it could be a target for future checkpoint inhibitor anti cancer drugs 10 PSGL 1 has been shown to bind to VISTA V domain Ig suppressor of T cell activation but this binding only occurs under acidic pH conditions pH lt 6 5 such as can be found in tumor microenvironments TME 11 In mice PSGL 1 seems to facilitate T cell exhaustion in tumors 12 References edit a b c GRCh38 Ensembl release 89 ENSG00000110876 Ensembl May 2017 a b c GRCm38 Ensembl release 89 ENSMUSG00000048163 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Mouse PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Entrez Gene SELPLG selectin P ligand Li F Wilkins PP Crawley S et al 1996 Post translational modifications of recombinant P selectin glycoprotein ligand 1 required for binding to P and E selectin J Biol Chem 271 6 3255 64 doi 10 1074 jbc 271 6 3255 PMID 8621728 Wilkins PP Moore KL McEver RP Cummings RD 1995 Tyrosine sulfation of P selectin glycoprotein ligand 1 is required for high affinity binding to P selectin J Biol Chem 270 39 22677 80 doi 10 1074 jbc 270 39 22677 PMID 7559387 Sako D Comess KM Barone KM et al 1995 A sulfated peptide segment at the amino terminus of PSGL 1 is critical for P selectin binding Cell 83 2 323 31 doi 10 1016 0092 8674 95 90173 6 PMID 7585949 S2CID 65420 Pouyani T Seed B 1995 PSGL 1 recognition of P selectin is controlled by a tyrosine sulfation consensus at the PSGL 1 amino terminus Cell 83 2 333 43 doi 10 1016 0092 8674 95 90174 4 PMID 7585950 S2CID 17480260 Immune Factor Seen to Control T Cell Checkpoints Involved in Spread of Cancers and Infections June 2016 Nature 2019 Oct 574 7779 565 570 doi 10 1038 s41586 019 1674 5 Epub 2019 Oct 23 PMID 31645726 Reviving exhausted T cells to tackle immunotherapy resistant cancers 2023Further reading editFurie B Furie BC 2004 Role of platelet P selectin and microparticle PSGL 1 in thrombus formation Trends in Molecular Medicine 10 4 171 8 doi 10 1016 j molmed 2004 02 008 PMID 15059608 Sako D Chang XJ Barone KM et al 1993 Expression cloning of a functional glycoprotein ligand for P selectin Cell 75 6 1179 86 doi 10 1016 0092 8674 93 90327 M PMID 7505206 S2CID 23786141 Moore KL Eaton SF Lyons DE et al 1994 The P selectin glycoprotein ligand from human neutrophils displays sialylated fucosylated O linked poly N acetyllactosamine J Biol Chem 269 37 23318 27 doi 10 1016 S0021 9258 17 31656 3 PMID 7521878 Veldman GM Bean KM Cumming DA et al 1995 Genomic organization and chromosomal localization of the gene encoding human P selectin glycoprotein ligand J Biol Chem 270 27 16470 5 doi 10 1074 jbc 270 27 16470 PMID 7541799 Maruyama K Sugano S 1994 Oligo capping a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides Gene 138 1 2 171 4 doi 10 1016 0378 1119 94 90802 8 PMID 8125298 Guyer DA Moore KL Lynam EB et al 1996 P selectin glycoprotein ligand 1 PSGL 1 is a ligand for L selectin in neutrophil aggregation Blood 88 7 2415 21 doi 10 1182 blood V88 7 2415 bloodjournal8872415 PMID 8839831 Goetz DJ Greif DM Ding H et al 1997 Isolated P selectin glycoprotein ligand 1 dynamic adhesion to P and E selectin J Cell Biol 137 2 509 19 doi 10 1083 jcb 137 2 509 PMC 2139768 PMID 9128259 Fuhlbrigge RC Kieffer JD Armerding D Kupper TS 1997 Cutaneous lymphocyte antigen is a specialized form of PSGL 1 expressed on skin homing T cells Nature 389 6654 978 81 Bibcode 1997Natur 389 978F doi 10 1038 40166 PMID 9353122 S2CID 4376296 Suzuki Y Yoshitomo Nakagawa K Maruyama K et al 1997 Construction and characterization of a full length enriched and a 5 end enriched cDNA library Gene 200 1 2 149 56 doi 10 1016 S0378 1119 97 00411 3 PMID 9373149 Snapp KR Ding H Atkins K et al 1998 A novel P selectin glycoprotein ligand 1 monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL 1 and blocks recognition of both P and L selectin Blood 91 1 154 64 doi 10 1182 blood V91 1 154 PMID 9414280 Bennett EP Hassan H Mandel U et al 1998 Cloning of a human UDP N acetyl alpha D Galactosamine polypeptide N acetylgalactosaminyltransferase that complements other GalNAc transferases in complete O glycosylation of the MUC1 tandem repeat J Biol Chem 273 46 30472 81 doi 10 1074 jbc 273 46 30472 PMID 9804815 Wimazal F Ghannadan M Muller MR et al 2000 Expression of homing receptors and related molecules on human mast cells and basophils a comparative analysis using multi color flow cytometry and toluidine blue immunofluorescence staining techniques Tissue Antigens 54 5 499 507 doi 10 1034 j 1399 0039 1999 540507 x PMID 10599889 Epperson TK Patel KD McEver RP Cummings RD 2000 Noncovalent association of P selectin glycoprotein ligand 1 and minimal determinants for binding to P selectin J Biol Chem 275 11 7839 53 doi 10 1074 jbc 275 11 7839 PMID 10713099 Andre P Spertini O Guia S et al 2000 Modification of P selectin glycoprotein ligand 1 with a natural killer cell restricted sulfated lactosamine creates an alternate ligand for L selectin Proc Natl Acad Sci U S A 97 7 3400 5 doi 10 1073 pnas 040569797 PMC 16251 PMID 10725346 Frenette PS Denis CV Weiss L et al 2000 P Selectin glycoprotein ligand 1 PSGL 1 is expressed on platelets and can mediate platelet endothelial interactions in vivo J Exp Med 191 8 1413 22 doi 10 1084 jem 191 8 1413 PMC 2193129 PMID 10770806 Woltmann G McNulty CA Dewson G et al 2000 Interleukin 13 induces PSGL 1 P selectin dependent adhesion of eosinophils but not neutrophils to human umbilical vein endothelial cells under flow Blood 95 10 3146 52 doi 10 1182 blood V95 10 3146 PMID 10807781 Tsuchihashi S Fondevila C Shaw GD et al 2006 Molecular characterization of rat leukocyte P selectin glycoprotein ligand 1 and effect of its blockade protection from ischemia reperfusion injury in liver transplantation J Immunol 176 1 616 24 doi 10 4049 jimmunol 176 1 616 PMID 16365457 External links editP selectin glycoprotein ligand 1 at the U S National Library of Medicine Medical Subject Headings MeSH This article incorporates text from the United States National Library of Medicine which is in the public domain Retrieved from https en wikipedia org w index php title P selectin glycoprotein ligand 1 amp oldid 1186053536, wikipedia, wiki, book, books, library,
