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Orthochromasia

In chemistry, orthochromasia is the property of a dye or stain to not change color on binding to a target, as opposed to metachromatic stains, which change color. The word is derived from the Greek orthos (correct, upright), and chromatic (color). Toluidine blue is an example of a partially orthochromatic dye, as it stains nucleic acids by its orthochromatic color (blue), but stains mast cell granules in its metachromatic color (red).

In spectral terms, orthochromasia refers to maintaining the position of spectral peaks, while metachromasia refers to a shift in wavelength, becoming either shorter or longer.

In photography, an orthochromatic light spectrum is one devoid of red light.

In biology, orthochromatic refers to the greyish staining because of acidophilic and basophilic mixture in the cell.

Orthochromatic photography edit

 
This WW II British Hawker Tornado prototype's 'Type A.1' RAF roundel's outermost, chrome yellow ring renders as dark gray, due to orthochromasia.
 
The Union Jack on orthochromatic emulsion at the South Magnetic Pole in 1909.

Orthochromatic photography refers to a photographic emulsion that is sensitive to only blue and green light, and thus can be processed with a red safelight. The increased blue sensitivity causes blue objects to appear lighter, and red ones darker. A cyan lens filter (which removes red light) can be used with standard panchromatic film to produce a similar effect.[1]

Orthochromatic films were first produced by Hermann Wilhelm Vogel in 1873 by adding small amounts of certain aniline-based dyes to photographic emulsions, which until that time had been sensitive to blue light only. This work was extended by others including Josef Maria Eder, who introduced the use of the red dye erythrosine in 1884.[2]

 
Comparison between orthochromatic and panchromatic film.

See also edit

References edit

  1. ^ Hulfish, David Sherrill (1970) [first published 1915]. Motion-Picture Work: The Literature of Cinema. Ayer Publishing. p. 206. ISBN 978-0405016172.
  2. ^ . Archived from the original on 2006-09-24. Retrieved 2012-09-27. {{cite book}}: |work= ignored (help)


orthochromasia, this, article, needs, additional, citations, verification, please, help, improve, this, article, adding, citations, reliable, sources, unsourced, material, challenged, removed, find, sources, news, newspapers, books, scholar, jstor, august, 200. This article needs additional citations for verification Please help improve this article by adding citations to reliable sources Unsourced material may be challenged and removed Find sources Orthochromasia news newspapers books scholar JSTOR August 2008 Learn how and when to remove this template message In chemistry orthochromasia is the property of a dye or stain to not change color on binding to a target as opposed to metachromatic stains which change color The word is derived from the Greek orthos correct upright and chromatic color Toluidine blue is an example of a partially orthochromatic dye as it stains nucleic acids by its orthochromatic color blue but stains mast cell granules in its metachromatic color red In spectral terms orthochromasia refers to maintaining the position of spectral peaks while metachromasia refers to a shift in wavelength becoming either shorter or longer In photography an orthochromatic light spectrum is one devoid of red light In biology orthochromatic refers to the greyish staining because of acidophilic and basophilic mixture in the cell Orthochromatic photography edit nbsp This WW II British Hawker Tornado prototype s Type A 1 RAF roundel s outermost chrome yellow ring renders as dark gray due to orthochromasia nbsp The Union Jack on orthochromatic emulsion at the South Magnetic Pole in 1909 Orthochromatic photography refers to a photographic emulsion that is sensitive to only blue and green light and thus can be processed with a red safelight The increased blue sensitivity causes blue objects to appear lighter and red ones darker A cyan lens filter which removes red light can be used with standard panchromatic film to produce a similar effect 1 Orthochromatic films were first produced by Hermann Wilhelm Vogel in 1873 by adding small amounts of certain aniline based dyes to photographic emulsions which until that time had been sensitive to blue light only This work was extended by others including Josef Maria Eder who introduced the use of the red dye erythrosine in 1884 2 nbsp Comparison between orthochromatic and panchromatic film See also editPanchromaticReferences edit Hulfish David Sherrill 1970 first published 1915 Motion Picture Work The Literature of Cinema Ayer Publishing p 206 ISBN 978 0405016172 Glossary Photography Orthochromatic Archived from the original on 2006 09 24 Retrieved 2012 09 27 a href Template Cite book html title Template Cite book cite book a work ignored help nbsp This photography related article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Orthochromasia amp oldid 1145828367, wikipedia, wiki, book, books, library,

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