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Nucleic acid methods

January 01, 1970

For protein methods, see Protein methods.

Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.

Purification edit

Quantification edit

Synthesis edit

Kinetics edit

Gene function edit

Other edit

See also edit

References edit

  1. ^ Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K. PMID 25295484.
  2. ^ Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology. 25 (9): 094009. Bibcode:2014Nanot..25i4009I. doi:10.1088/0957-4484/25/9/094009. PMID 24522116. S2CID 206070778.
  3. ^ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics. 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
  4. ^ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques. 19 (3): 151–158. PMC 2563918. PMID 19137101.
  5. ^ Hannon, Gregory J. (July 2002). "RNA interference". Nature. 418 (6894): 244–251. Bibcode:2002Natur.418..244H. doi:10.1038/418244a. ISSN 1476-4687. PMID 12110901. S2CID 4426281.

External links edit

    January 01, 1970
    nucleic, acid, methods, protein, methods, protein, methods, techniques, used, study, nucleic, acids, contents, purification, quantification, synthesis, kinetics, gene, function, other, also, references, external, linkspurification, editdna, extraction, phenol,. For protein methods see Protein methods Nucleic acid methods are the techniques used to study nucleic acids DNA and RNA Contents 1 Purification 2 Quantification 3 Synthesis 4 Kinetics 5 Gene function 6 Other 7 See also 8 References 9 External linksPurification editDNA extraction Phenol chloroform extraction Minicolumn purification RNA extraction Boom method Synchronous coefficient of drag alteration SCODA DNA purificationQuantification editAbundance in weight spectroscopic nucleic acid quantitation Absolute abundance in number real time polymerase chain reaction quantitative PCR High throughput relative abundance DNA microarray High throughput absolute abundance serial analysis of gene expression SAGE Size gel electrophoresisSynthesis editDe novo oligonucleotide synthesis Amplification polymerase chain reaction PCR Kinetics editMulti parametric surface plasmon resonance 1 2 Dual polarization interferometry 3 Quartz crystal microbalance with dissipation monitoring QCM D 4 Gene function editRNA interference 5 Other editBisulfite sequencing DNA sequencing Expression cloning Fluorescence in situ hybridization Lab on a chip Comparison of nucleic acid simulation software Northern blot Nuclear run on assay Radioactivity in the life sciences Southern blot Differential centrifugation sucrose gradient Toeprinting assay Several bioinformatics methods as seen in list of RNA structure prediction softwareSee also editCSH Protocols Current ProtocolsReferences edit Tang Wei Hu Shichao Wang Huaming Zhao Yan Li Na Liu Feng 23 September 2014 A universal molecular translator for non nucleic acid targets that enables dynamic DNA assemblies and logic operations Chem Commun 50 92 14352 14355 doi 10 1039 C4CC07041K PMID 25295484 Ihalainen Petri Pettersson Fredrik Pesonen Markus Viitala Tapani Maattanen Anni Osterbacka Ronald Peltonen Jouko 7 March 2014 An impedimetric study of DNA hybridization on paper supported inkjet printed gold electrodes Nanotechnology 25 9 094009 Bibcode 2014Nanot 25i4009I doi 10 1088 0957 4484 25 9 094009 PMID 24522116 S2CID 206070778 Berney H Oliver K 2005 Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation Biosensors and Bioelectronics 21 4 618 626 doi 10 1016 j bios 2004 12 024 PMID 16202875 Dixon Matthew C July 2008 Quartz Crystal Microbalance with Dissipation Monitoring Enabling Real Time Characterization of Biological Materials and Their Interactions Journal of Biomolecular Techniques 19 3 151 158 PMC 2563918 PMID 19137101 Hannon Gregory J July 2002 RNA interference Nature 418 6894 244 251 Bibcode 2002Natur 418 244H doi 10 1038 418244a ISSN 1476 4687 PMID 12110901 S2CID 4426281 External links editProtocols for Recombinant DNA Isolation Cloning and Sequencing Retrieved from https en wikipedia org w index php title Nucleic acid methods amp oldid 1187433882, wikipedia, wiki, book, books, library,

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