Normandina pulchella
Normandina pulchella, commonly known as the elf-ear lichen or blue heart, is a species of squamulose lichen in the family Verrucariaceae. This cosmopolitan species is widely distributed across both hemispheres, where it thrives in moist microhabitats. It favours moss-covered deciduous trees and rocks, often colonising over mosses and bryophytes. It occasionally grows on bare bark and on other lichens. Distinctive features of N. pulchella include its bluish-green squamules (scales) with sharply raised margins, non-reactivity to standard chemical spot tests, and growth in humid habitats. Initially, Nannochloris normandinae, a green alga, was thought to be its photobiont. However, recent studies have revised this understanding, now suggesting Diplosphaera as the algal partner.
| Normandina pulchella | |
|---|---|
| Scientific classification | |
| Domain: | Eukaryota |
| Kingdom: | Fungi |
| Division: | Ascomycota |
| Class: | Eurotiomycetes |
| Order: | Verrucariales |
| Family: | Verrucariaceae |
| Genus: | Normandina |
| Species: | N. pulchella |
| Binomial name | |
| Normandina pulchella | |
| Synonyms[2] | |
First named and scientifically described by the English botanist William Borrer in 1831, the clarification of Normandina pulchella's place within the Verrucariaceae, facilitated by molecular phylogenetics analysis in 2010, resolved long-standing taxonomic uncertainties. Prior classifications had varied widely, placing N. pulchella within groups such as the Basidiomycota (i.e., as a basidiolichen) and Fungi incertae sedis, largely due to differing interpretations of the perithecia (fruiting bodies) found within the lichen. These discrepancies stemmed from confusion over whether the perithecia belonged to the lichen itself or were instead associated with a parasitic lichenicolous fungus.
Systematics edit
Taxonomic history edit
The lichen was originally formally described in 1831 by the English botanist William Borrer, who classified it within the genus Verrucaria. He coined the vernacular name "little filmy-leaved Verrucaria" due to its distinctive morphology.[3] The specific epithet pulchella is the Latin diminutive of pulchra, 'beautiful' or 'fair'.[4] He observed the lichen to be characterised by thin, membranous, greenish-grey, leaf-like scales, with these features transitioning from smooth, rounded forms to crowded, waved, and lobed configurations, whilst adorned with powdery granules. The underside of these scales is distinguished by a pale brown colour and woolly fibres. Borrer described the lichen's tubercles as nearly globular and black. These tubercles reveal only their apex through the thallus surface, exposing a gelatinous, brownish nucleus with a central pore. He noted the lichen's frequent occurrence on mossy trees in Sussex, and that it thrived on the leafy liverwort species Jungermannia dilatata, forming wide but often interrupted patches. Ellen Hutchins was credited by Borrer for initially discovering the species on a mountain near Bantry (Ireland), growing on Lichen plumbeus on heath stems. He remarked on the lichen's uniqueness: "This curious little production is so unlike to every other Lichen, that its very genus must have remained doubtful but for Miss Hutchins's fortunate discovery of the tubercles. Acharius, to whom Sussex specimens were communicated, thought it a Thelephora, thus excluding it even from the natural order to which we hold it to belong." Despite its prevalence in Sussex, Borrer mentioned that it appeared to have been overlooked elsewhere.[5] In another publication that year, Borrer proposed to transfer it to the genus Endocarpon. However, this was nomenclaturally invalid because the name had already been for a different species, violating the International Code of Botanical Nomenclature guidelines.[3] William Nylander transferred the taxon to the genus Normandina in 1861.[6]
Classification edit
Recent advancements have clarified the classification of Normandina pulchella, addressing ambiguities and refining its placement within the systematic taxonomy. Initially, perithecia found within the thallus led to confusion, as these were later attributed to the parasitic fungus Sphaerulina chlorococca rather than to Normandina pulchella itself. This misunderstanding contributed to significant taxonomic challenges regarding the lichen's classification. Historically, N. pulchella was often classified as a basidiolichen, largely due to its morphological similarities with Coriscium viride, associated with the basidiomycete Omphalina hudsoniana. However, the discovery that Normandina lacks dolipore septa — key features of basidiomycetes — cast doubt on its classification as a basidiolichen. Despite this, the exact systematic position of Normandina remained unresolved.[7]
A cytological study revealed that Normandina pulchella has a complex structure with separate fungal and algal layers, a characteristic of heteromerous thalli. This structure plays a key role in its life processes, distinguishing it from basidiolichens. Specifically, the medulla, or inner layer, contains hyaline (transparent) fungal filaments entwined around small clusters of algal cells. These cells have thick walls and a singular, lobate chloroplast containing a distinct metameric pyrenoid – a structure assisting in starch formation, along with small starch grains and fat-containing plastoglobuli.[7]
The fungal partner, or mycobiont, was found to have hyphae featuring simple perforated septa, a type of internal cell division within the hyphae, accompanied by Woronin bodies – organelles unique to ascomycetes, a large fungal group. Additionally, the close yet non-invasive relationship between the fungal and algal cells suggested a mutualistic association typical of ascolichens. The exclusive presence of Woronin bodies and the characteristics of the algal partner align with traits typically found in ascomycetes and their associated algae in ascolichens. This evidence collectively shifted Normandina pulchella away from being classified as a basidiolichen, as some previous studies suggested, firmly placing it within the ascolichens.[7]
In 2010, Lucia Muggia and colleagues employed molecular phylogenetics analysis to conclusively classify Normandina pulchella within the family Verrucariaceae, resolving longstanding classification debates.[8]
Common names edit
In addition to Borrer's original suggestion ("little filmy-leaved Verrucaria"), other vernacular names that have been used to refer to this lichen are "blue heart",[9] and "elf ear lichen".[10]
Description edit
Normandina pulchella has a squamulose (scaly) thallus, composed of small, scale-like formations termed squamules. The squamules display colours ranging from glaucous—a bluish-green or grey—to pale grey or greenish-grey, intensifying to a richer green when moistened. Each squamule can span up to 5 mm across and may include one or more broadly rounded lobes, each up to 1.7 mm in diameter, reminiscent of shell or ear shapes. The upper surface of these lobes features concentric ridges, while the edges are sharply defined and raised, typically spanning 50–100 micrometres (µm) in width. The squamules may be dispersed or densely packed across the lichen's surface.[11] The thallus is closely appressed to the substrate.[10]
For vegetative reproduction, N. pulchella develops soralia—structures on the lobe surfaces and edges—that discharge granular particles known as soredia. These soralia are green or match the colour of the lobes and contain granular soredia measuring 40–80 µm in diameter. The underside of the lichen presents a whitish, slightly felted (tomentose) appearance and adheres to its substrate through numerous fungal strands, or hyphae.[11] Rhizines do not occur in this species.[10]
Its spore-producing structures, or ascomata, resemble those in related species but are distinguished by their uniformly pigmented, cohesive nature under microscopic analysis. The ascospores are typically 29–37 by 6–7 µm, mostly with seven internal partitions, known as septa. Chemical analysis, particularly thin-layer chromatography, identify zeorin as a secondary metabolite (lichen product), yet N. pulchella remains unresponsive to standard chemical spot tests.[11]
Similar species edit
The squamules of Lichenomphalina hudsoniana share the neat, sharply defined edges characteristic of N. pulchella. However, they can be distinguished by their lack of soralia, the presence of both upper and lower cortices, and adaptation to arctic-alpine environments, typically growing on peaty soil or decaying wood. In contrast, N. pulchella tends to develop more pronounced soralia in shaded, humid settings, diverging from the preferred habitats of Lichenomphalina hudsoniana. Additionally, the fruiting bodies of N. pulchella are found to be more prevalent and larger in tropical regions compared to temperate ones, and they often do not contain soredia. This suggests that environmental factors or underlying taxonomic differences may influence the observed variations between these species in different locales.[11][10]
Photobiont edit
In 1981, Elisabeth Tschermak-Woess identified Nannochloris normandinae as the photobiont partner, the algae associated with Normandina pulchella.[12] Subsequent studies, however, have been less definitive about the role of Nannochloris.[13] By 2011, research by Thüs and colleagues revealed that Diplosphaera, not Nannochloris, was present in ten examined Normandina specimens.[14] More recently, Pröschold & Darienko (2020) reclassified Nannochloris normandinae as synonymous with Diplosphaera chodatii of the order Prasiolales,[15] further complicating the identification of the true photobiont in N. pulchella and suggesting that previous attributions to Nannochloris may be incorrect.[14]
Habitat and distribution edit
Normandina pulchella has a cosmopolitan distribution, growing across diverse climates and regions.[11] The Swedish lichenologist Gunnar Degelius, in his 1934 phytogeographical study, highlights the species' oceanic distribution in Europe. It predominantly occupies coastal areas in Northern Europe, including Scandinavia and the British Isles, and extends to Austria, Bavaria, France, Czechoslovakia, and select Mediterranean locales.[16] In contrast, William Louis Culberson and Mason Hale's 1966 analysis of its North American presence noted its prevalence in the mountainous western regions and the Appalachian foothills, without indicating an oceanic distribution pattern.[17] Its range in North America extends north to Alaska,[18] although in 2022 Alan Orange showed that some collections from there represent a different species found in the Americas, provisionally named N. 'americana'.[19]
Ecologically, Normandina pulchella favours moss-covered deciduous trees and rocks within woodlands and parks, often colonising over mosses, bryophytes, and occasionally bare bark.[11] It also often grows on other lichens, particularly those that contain cyanobacteria,[20] such as Fuscopannaria, Pannaria, Parmeliella, Pectenia, and Peltigera. Its presence is increasing in southern and western Britain and throughout Ireland, reflecting a broadening distribution.[11]
It is listed as a vulnerable in the Finnish Regional Red List because of its small known population.[21]
Species interactions edit
Among the lichenicolous (lichen-dwelling) fungi specifically associated with Normandina pulchella are several species that have unique interactions. Capronia normandinae is characterised by its black, superficial, hair-like structures known as setose perithecia. Cladophialophora normandinae is distinguished by its black fruiting bodies, termed sporodochia, which play a role in its reproductive cycle. Additionally, Tremella normandinae is noted for producing pale, swollen growths, referred to as galls, indicative of its parasitic relationship with the lichen. Another parasite, Globosphaeria jamesii, also interacts with Normandina pulchella, further contributing to the diversity of its ecological associations. Moreover, Lawreymyces pulchellae has an endolichenic relationship, residing within the lichen's structure.[11][22]
References edit
- ^ Nylander, W. (1855). "Additamentum in floram cryptogamicam Chilensem". Annales des Sciences Naturelles. Botanique. 4 (in Latin). 3: 145–187 [151].
- ^ "Synonymy. Current Name: Normandina pulchella (Borrer) Nyl., Annls Sci. Nat., Bot., sér. 4 15: 382 (1861)". Species Fungorum. Retrieved 27 March 2024.
- ^ a b "Record Details: Endocarpon pulchellum Borrer, in Hooker & Sowerby, Suppl. Engl. Bot. 1: tab. 2602 (1831)". Index Fungorum. Retrieved 27 March 2024.
- ^ Bomfleur, Benjamin; Grimm, Guido W.; McLoughlin, Stephen (2015). "Osmunda pulchella sp. nov. from the Jurassic of Sweden—reconciling molecular and fossil evidence in the phylogeny of modern royal ferns (Osmundaceae)". BMC Evolutionary Biology. 15: e15. doi:10.1186/s12862-015-0400-7. PMC 4487210. PMID 26123220.
- ^ Borrer, William (1831). Supplement to the English Botany of the late Sir J. E. Smith and Mr. Sowerby. Vol. 1. London: J. D. C. and C. E. Sowerby. tab. 2602, fig. 1.
- ^ Nylander, W. (1861). "Additamentum ad lichenographiam Andium Boliviensium". Annales des Sciences Naturelles. Botanique. 4 (in Latin). 15: 365–382 [382].
- ^ a b c Mares, Donatello; Fasulo, Maria P.; Bruni, Alessandro (1993). "Contribution to the study of Normandina pulchella: a cytological approach". Orsis. 8: 33–40.
- ^ Muggia, Lucia; Gueidan, Cécile; Grube, Martin (2010). "Phylogenetic placement of some morphologically unusual members of Verrucariales". Mycologia. 102 (4): 835–846. doi:10.3852/09-153. PMID 20648751.
- ^ Tripp, Erin A.; Lendemer, James C. (2020). Field Guide to the Lichens of Great Smoky Mountains National Park. Knoxville: The University of Tennessee Press. pp. 308–309. ISBN 978-1-62190-514-1.
- ^ a b c d McMullin, R. Troy (2023). Lichens. The Macrolichens of Ontario and the Great Lakes Region of the United States. Firefly Books. p. 311. ISBN 978-0-228-10369-1.
- ^ a b c d e f g h Orange, A.; Cannon, P.; Prieto, M.; Coppins, B.; Sanderson, N.; Simkin, J. (2023). Verrucariales: Verrucariaceae, including the genera Agonimia, Atla, Bagliettoa, Catapyrenium, Dermatocarpon, Endocarpon, Henrica, Heteroplacidium, Hydropunctaria, Involucropyrenium, Merismatium, Nesothele, Normandina, Parabagliettoa, Placidopsis, Placidium, Placopyrenium, Polyblastia, Psoroglaena, Sporodictyon, Staurothele, Thelidium, Trimmatothele, Verrucaria, Verrucula, Verruculopsis and Wahlenbergiella (PDF). Revisions of British and Irish Lichens. Vol. 31. British Lichen Society. p. 43.
- ^ Tschermak-Woess, E. (1981). "Zur Kenntnis der Phycobionten von Lobaria linita und Normandina pulchella" [On the knowledge of the phycobionts of Lobaria linita and Normandina pulchella]. Nova Hedwigia (in German). 35: 63–73.
- ^ Lohtander, Katileena; Oksanen, Ilona; Rikkinen, Jouko (2003). "Genetic diversity of green algal and cyanobacterial photobionts in Nephroma (Peltigerales)". The Lichenologist. 35 (4): 325–339. doi:10.1016/S0024-2829(03)00051-3.
- ^ a b Thüs, Holger; Muggia, Lucia; Pérez-Ortega, Sergio; Favero-Longo, Sergio E.; Joneson, Suzanne; O’Brien, Heath; Nelsen, Matthew P.; Duque-Thüs, Rhinaixa; Grube, Martin; Friedl, Thomas; Brodie, Juliet; Andrew, Carrie J.; Lücking, Robert; Lutzoni, François; Gueidan, Cécile (2011). "Revisiting photobiont diversity in the lichen family Verrucariaceae (Ascomycota)". European Journal of Phycology. 46 (4): 399–415. Bibcode:2011EJPhy..46..399T. doi:10.1080/09670262.2011.629788.
- ^ Pröschold, Thomas; Darienko, Tatyana (2020). "The green puzzle Stichococcus (Trebouxiophyceae, Chlorophyta): new generic and species concept among this widely distributed genus". Phytotaxa. 441 (1): 113–142. doi:10.11646/phytotaxa.441.2.2.
- ^ Degelius, Gunnar (1935). Das oceanische Element der Strauch- und Laubflechtenflora von Skandinavien [The oceanic element of the shrub and leaf lichen flora of Scandinavia]. Acta Phytogeographica Suecica (in German). Vol. 7.
- ^ Culberson, William Louis; Mason E., Hale (1966). "The range of Normandina pulchella in North America". The Bryologist. 69 (3): 365–367. doi:10.2307/3240836. JSTOR 3240836.
- ^ Spribille, Toby; Fryday, Alan M.; Hampton-Miller, Celia J.; Ahti, Teuvo; Dillman, Karen; Thor, Göran; Tonsberg, Tor; Schirokauer, Dave, eds. (2023). Compendium of the Lichens and Associated Fungi of Alaska. Bibliothecia Lichenologica. J. Cramer. p. 278. doi:10.1127/bibl_lich/2023/112. ISBN 978-3-443-58093-3.
- ^ Orange, Alan (2022). "The crustose species of Normandina Verrucariaceae)". The Lichenologist. 54 (6): 371–378. doi:10.1017/s0024282922000317.
- ^ Sharnoff, Stephen (2014). A Field Guide to California Lichens. New Haven/London: Yale University Press. p. 357. ISBN 978-0-300-19500-2.
- ^ Hyvärinen, Esko; Juslén, Aino; Kemppainen, Eija; Uddström, Annika; Liukko, Ulla-Maija, eds. (2019). Suomen lajien uhanalaisuus – Punainen kirja 2019. The 2019 Red List of Finnish Species (Report). Ministry of the Environment. Finnish Environment Institute. p. 292. ISBN 978-952-11-4974-0.
- ^ Diederich, Paul; Lawrey, James D.; Ertz, Damien (2018). "The 2018 classification and checklist of lichenicolous fungi, with 2000 non-lichenized, obligately lichenicolous taxa". The Bryologist. 121 (3): 385, 392. doi:10.1639/0007-2745-121.3.340.