The protein encoded by this gene was first identified by its binding to the APC (Adenomatous polyposis coli) protein which is often mutated in familial and sporadic forms of colorectal cancer.[9]
Immunofluorescence has demonstrated that EB1 localizes to the centrosome, mitotic spindle, and distal tips of cytoplasmic microtubules. Throughout the cell cycle, EB1 proteins situate on the microtubule plus ends, which is why EB1 is categorized as a microtubule plus end tracking protein(+TIP protein).[10]
The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.[8]
^ abcGRCh38: Ensembl release 89: ENSG00000101367 – Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000027479 – Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Su LK, Burrell M, Hill DE, Gyuris J, Brent R, Wiltshire R, Trent J, Vogelstein B, Kinzler KW (Aug 1995). "APC binds to the novel protein EB1". Cancer Res. 55 (14): 2972–7. PMID 7606712.
^Berrueta L, Kraeft SK, Tirnauer JS, Schuyler SC, Chen LB, Hill DE, Pellman D, Bierer BE (Sep 1998). "The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules". Proc. Natl. Acad. Sci. USA. 95 (18): 10596–601. Bibcode:1998PNAS...9510596B. doi:10.1073/pnas.95.18.10596. PMC27940. PMID 9724749.
^Nakamura M, Zhou XZ, Lu KP (Jul 2001). "Critical role for the EB1 and APC interaction in the regulation of microtubule polymerization". Curr. Biol. 11 (13): 1062–7. Bibcode:2001CBio...11.1062N. doi:10.1016/S0960-9822(01)00297-4. PMID 11470413. S2CID 14122895.
^ ab"Entrez Gene: MAPRE1 microtubule-associated protein, RP/EB family, member 1".
^Su LK, Burrell M, Hill DE, Gyuris J, Brent R, Wiltshire R, Trent J, Vogelstein B, Kinzler KW (July 1995). "APC binds to the novel protein EB1". Cancer Research. 55 (14): 2972–7. PMID 7606712.
^Tirnauer JS, Bierer BE (May 2000). "EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability". The Journal of Cell Biology. 149 (4): 761–6. doi:10.1083/jcb.149.4.761. PMC2174556. PMID 10811817.
^Nakamura M, Zhou XZ, Kishi S, Lu KP (Mar 2002). "Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle". FEBS Lett. 514 (2–3): 193–8. doi:10.1016/S0014-5793(02)02363-3. PMID 11943150. S2CID 2579290.
Further readingedit
Tirnauer JS, Bierer BE (2000). "EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability". J. Cell Biol. 149 (4): 761–6. doi:10.1083/jcb.149.4.761. PMC2174556. PMID 10811817.
Morrison EE, Wardleworth BN, Askham JM, Markham AF, Meredith DM (1999). "EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle". Oncogene. 17 (26): 3471–7. doi:10.1038/sj.onc.1202247. PMID 10030671.
Juwana JP, Henderikx P, Mischo A, Wadle A, Fadle N, Gerlach K, Arends JW, Hoogenboom H, Pfreundschuh M, Renner C (1999). "EB/RP gene family encodes tubulin binding proteins". Int. J. Cancer. 81 (2): 275–84. doi:10.1002/(SICI)1097-0215(19990412)81:2<275::AID-IJC18>3.0.CO;2-Z. PMID 10188731. S2CID 33232892.
Berrueta L, Tirnauer JS, Schuyler SC, Pellman D, Bierer BE (1999). "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain". Curr. Biol. 9 (8): 425–8. Bibcode:1999CBio....9..425B. doi:10.1016/S0960-9822(99)80190-0. PMID 10226031. S2CID 14191776.
Nakagawa H, Koyama K, Murata Y, Morito M, Akiyama T, Nakamura Y (2000). "EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue". Oncogene. 19 (2): 210–6. doi:10.1038/sj.onc.1203308. PMID 10644998.
Askham JM, Moncur P, Markham AF, Morrison EE (2000). "Regulation and function of the interaction between the APC tumour suppressor protein and EB1". Oncogene. 19 (15): 1950–8. doi:10.1038/sj.onc.1203498. PMID 10773885.
Mimori-Kiyosue Y, Shiina N, Tsukita S (2000). "The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules". Curr. Biol. 10 (14): 865–8. Bibcode:2000CBio...10..865M. doi:10.1016/S0960-9822(00)00600-X. PMID 10899006. S2CID 18926885.
Zhou XZ, Lu KP (2001). "The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor". Cell. 107 (3): 347–59. CiteSeerX10.1.1.326.1815. doi:10.1016/S0092-8674(01)00538-4. PMID 11701125. S2CID 6822193.
Nakamura M, Zhou XZ, Kishi S, Lu KP (2002). "Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle". FEBS Lett. 514 (2–3): 193–8. doi:10.1016/S0014-5793(02)02363-3. PMID 11943150. S2CID 2579290.
Askham JM, Vaughan KT, Goodson HV, Morrison EE (2003). "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome". Mol. Biol. Cell. 13 (10): 3627–45. doi:10.1091/mbc.E02-01-0061. PMC129971. PMID 12388762.
Ligon LA, Shelly SS, Tokito M, Holzbaur EL (2003). "The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization". Mol. Biol. Cell. 14 (4): 1405–17. doi:10.1091/mbc.E02-03-0155. PMC153110. PMID 12686597.
Hayashi I, Ikura M (2003). "Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1)". J. Biol. Chem. 278 (38): 36430–4. doi:10.1074/jbc.M305773200. PMID 12857735.
Bu W, Su LK (2004). "Characterization of functional domains of human EB1 family proteins". J. Biol. Chem. 278 (50): 49721–31. doi:10.1074/jbc.M306194200. PMID 14514668.
Bieling P, Laan L, Schek H, Munteanu EL, Sandblad L, Dogterom M, Brunner D, Surrey T (2007). "Reconstitution of a microtubule plus-end tracking system in vitro". Nature. 450 (7172): 1100–5. Bibcode:2007Natur.450.1100B. doi:10.1038/nature06386. PMID 18059460. S2CID 4355669.
Maurer SP, Fourniol FJ, Bohner G, Moores CA, Surrey T (2012). "EBs recognize a nucleotide-dependent structural cap at growing microtubule ends". Cell. 149 (2): 371–82. doi:10.1016/j.cell.2012.02.049. PMC3368265. PMID 22500803.
Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y (2012). "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells". PLOS ONE. 7 (12): e51442. Bibcode:2012PLoSO...751442N. doi:10.1371/journal.pone.0051442. PMC3520847. PMID 23251535.
Zanic M, Widlund PO, Hyman AA, Howard J (2013). "Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels". Nat. Cell Biol. 15 (6): 688–93. doi:10.1038/ncb2744. PMID 23666085. S2CID 3025200.
Jie, Chen (2014). "Phosphoregulation of the dimerization and functions of end-binding protein 1". Protein & Cell. 5 (10): 795–799. doi:10.1007/s13238-014-0081-9. PMC4180461. PMID 25048701.
This article on a gene on human chromosome 20 is a stub. You can help Wikipedia by expanding it.
mapre1, microtubule, associated, protein, family, member, protein, that, humans, encoded, gene, available, structurespdbortholog, search, pdbe, rcsblist, codes1pa7, 1txq, 1ueg, 1vka, 1wu9, 1yib, 1yig, 2hkq, 2hl3, 2hl5, 2qjz, 2r8u, 3gjo, 3mtu, 3tq7, 4xa1, 4xa3,. Microtubule associated protein RP EB family member 1 is a protein that in humans is encoded by the MAPRE1 gene 5 6 7 8 MAPRE1Available structuresPDBOrtholog search PDBe RCSBList of PDB id codes1PA7 1TXQ 1UEG 1VKA 1WU9 1YIB 1YIG 2HKQ 2HL3 2HL5 2QJZ 2R8U 3GJO 3MTU 3TQ7 4XA1 4XA3 4XA6IdentifiersAliasesMAPRE1 EB1 microtubule associated protein RP EB family member 1External IDsOMIM 603108 MGI 891995 HomoloGene 56129 GeneCards MAPRE1Gene location Human Chr Chromosome 20 human 1 Band20q11 21Start32 819 954 bp 1 End32 850 405 bp 1 Gene location Mouse Chr Chromosome 2 mouse 2 Band2 H1 2 75 95 cMStart153 583 194 bp 2 End153 615 230 bp 2 RNA expression patternBgeeHumanMouse ortholog Top expressed insecondary oocyteganglionic eminenceislet of Langerhansmonocytebone marrowpancreatic epithelial cellsmooth muscle tissuepancreatic ductal cellappendixvisceral pleuraTop expressed inmedial ganglionic eminenceretinal pigment epitheliumdermisatrioventricular valvemaxillary prominenceatriumendocardial cushionepithelium of stomachmolarsciatic nerveMore reference expression dataBioGPSMore reference expression dataGene ontologyMolecular functionprotein binding microtubule binding protein C terminus binding microtubule plus end binding identical protein binding RNA binding cadherin binding protein kinase bindingCellular componentmicrotubule plus end microtubule cytoskeleton microtubule organizing center cortical microtubule cytoskeleton cell projection membrane Golgi apparatus centrosome cytoskeleton cell projection spindle cytoplasmic microtubule cytosol microtubule cytoplasm focal adhesion spindle midzone mitotic spindle astral microtubule endBiological processG2 M transition of mitotic cell cycle positive regulation of microtubule plus end binding cell population proliferation negative regulation of microtubule polymerization cell division cell cycle protein localization to microtubule sister chromatid cohesion negative regulation of microtubule binding positive regulation of cell migration ciliary basal body plasma membrane docking regulation of microtubule cytoskeleton organization regulation of G2 M transition of mitotic cell cycle protein localization cell migration regulation of microtubule polymerization or depolymerization positive regulation of microtubule polymerization spindle assembly protein localization to microtubule plus endSources Amigo QuickGOOrthologsSpeciesHumanMouseEntrez2291913589EnsemblENSG00000101367ENSMUSG00000027479UniProtQ15691Q61166RefSeq mRNA NM 012325NM 007896RefSeq protein NP 036457NP 031922Location UCSC Chr 20 32 82 32 85 MbChr 2 153 58 153 62 MbPubMed search 3 4 WikidataView Edit HumanView Edit Mouse Contents 1 Function 2 Interactions 3 References 4 Further readingFunction editThe protein encoded by this gene was first identified by its binding to the APC Adenomatous polyposis coli protein which is often mutated in familial and sporadic forms of colorectal cancer 9 Immunofluorescence has demonstrated that EB1 localizes to the centrosome mitotic spindle and distal tips of cytoplasmic microtubules Throughout the cell cycle EB1 proteins situate on the microtubule plus ends which is why EB1 is categorized as a microtubule plus end tracking protein TIP protein 10 The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein Because of these associations it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability This gene is a member of the RP EB family 8 Interactions editMAPRE1 has been shown to interact with TERF1 11 References edit a b c GRCh38 Ensembl release 89 ENSG00000101367 Ensembl May 2017 a b c GRCm38 Ensembl release 89 ENSMUSG00000027479 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Mouse PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Su LK Burrell M Hill DE Gyuris J Brent R Wiltshire R Trent J Vogelstein B Kinzler KW Aug 1995 APC binds to the novel protein EB1 Cancer Res 55 14 2972 7 PMID 7606712 Berrueta L Kraeft SK Tirnauer JS Schuyler SC Chen LB Hill DE Pellman D Bierer BE Sep 1998 The adenomatous polyposis coli binding protein EB1 is associated with cytoplasmic and spindle microtubules Proc Natl Acad Sci USA 95 18 10596 601 Bibcode 1998PNAS 9510596B doi 10 1073 pnas 95 18 10596 PMC 27940 PMID 9724749 Nakamura M Zhou XZ Lu KP Jul 2001 Critical role for the EB1 and APC interaction in the regulation of microtubule polymerization Curr Biol 11 13 1062 7 Bibcode 2001CBio 11 1062N doi 10 1016 S0960 9822 01 00297 4 PMID 11470413 S2CID 14122895 a b Entrez Gene MAPRE1 microtubule associated protein RP EB family member 1 Su LK Burrell M Hill DE Gyuris J Brent R Wiltshire R Trent J Vogelstein B Kinzler KW July 1995 APC binds to the novel protein EB1 Cancer Research 55 14 2972 7 PMID 7606712 Tirnauer JS Bierer BE May 2000 EB1 proteins regulate microtubule dynamics cell polarity and chromosome stability The Journal of Cell Biology 149 4 761 6 doi 10 1083 jcb 149 4 761 PMC 2174556 PMID 10811817 Nakamura M Zhou XZ Kishi S Lu KP Mar 2002 Involvement of the telomeric protein Pin2 TRF1 in the regulation of the mitotic spindle FEBS Lett 514 2 3 193 8 doi 10 1016 S0014 5793 02 02363 3 PMID 11943150 S2CID 2579290 Further reading editTirnauer JS Bierer BE 2000 EB1 proteins regulate microtubule dynamics cell polarity and chromosome stability J Cell Biol 149 4 761 6 doi 10 1083 jcb 149 4 761 PMC 2174556 PMID 10811817 Morrison EE Wardleworth BN Askham JM Markham AF Meredith DM 1999 EB1 a protein which interacts with the APC tumour suppressor is associated with the microtubule cytoskeleton throughout the cell cycle Oncogene 17 26 3471 7 doi 10 1038 sj onc 1202247 PMID 10030671 Juwana JP Henderikx P Mischo A Wadle A Fadle N Gerlach K Arends JW Hoogenboom H Pfreundschuh M Renner C 1999 EB RP gene family encodes tubulin binding proteins Int J Cancer 81 2 275 84 doi 10 1002 SICI 1097 0215 19990412 81 2 lt 275 AID IJC18 gt 3 0 CO 2 Z PMID 10188731 S2CID 33232892 Berrueta L Tirnauer JS Schuyler SC Pellman D Bierer BE 1999 The APC associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain Curr Biol 9 8 425 8 Bibcode 1999CBio 9 425B doi 10 1016 S0960 9822 99 80190 0 PMID 10226031 S2CID 14191776 Nakagawa H Koyama K Murata Y Morito M Akiyama T Nakamura Y 2000 EB3 a novel member of the EB1 family preferentially expressed in the central nervous system binds to a CNS specific APC homologue Oncogene 19 2 210 6 doi 10 1038 sj onc 1203308 PMID 10644998 Askham JM Moncur P Markham AF Morrison EE 2000 Regulation and function of the interaction between the APC tumour suppressor protein and EB1 Oncogene 19 15 1950 8 doi 10 1038 sj onc 1203498 PMID 10773885 Mimori Kiyosue Y Shiina N Tsukita S 2000 The dynamic behavior of the APC binding protein EB1 on the distal ends of microtubules Curr Biol 10 14 865 8 Bibcode 2000CBio 10 865M doi 10 1016 S0960 9822 00 00600 X PMID 10899006 S2CID 18926885 Zhou XZ Lu KP 2001 The Pin2 TRF1 interacting protein PinX1 is a potent telomerase inhibitor Cell 107 3 347 59 CiteSeerX 10 1 1 326 1815 doi 10 1016 S0092 8674 01 00538 4 PMID 11701125 S2CID 6822193 Nakamura M Zhou XZ Kishi S Lu KP 2002 Involvement of the telomeric protein Pin2 TRF1 in the regulation of the mitotic spindle FEBS Lett 514 2 3 193 8 doi 10 1016 S0014 5793 02 02363 3 PMID 11943150 S2CID 2579290 Askham JM Vaughan KT Goodson HV Morrison EE 2003 Evidence that an interaction between EB1 and p150 Glued is required for the formation and maintenance of a radial microtubule array anchored at the centrosome Mol Biol Cell 13 10 3627 45 doi 10 1091 mbc E02 01 0061 PMC 129971 PMID 12388762 Tirnauer JS Canman JC Salmon ED Mitchison TJ 2003 EB1 targets to kinetochores with attached polymerizing microtubules Mol Biol Cell 13 12 4308 16 doi 10 1091 mbc E02 04 0236 PMC 138635 PMID 12475954 Ligon LA Shelly SS Tokito M Holzbaur EL 2003 The microtubule plus end proteins EB1 and dynactin have differential effects on microtubule polymerization Mol Biol Cell 14 4 1405 17 doi 10 1091 mbc E02 03 0155 PMC 153110 PMID 12686597 Hayashi I Ikura M 2003 Crystal structure of the amino terminal microtubule binding domain of end binding protein 1 EB1 J Biol Chem 278 38 36430 4 doi 10 1074 jbc M305773200 PMID 12857735 Bu W Su LK 2004 Characterization of functional domains of human EB1 family proteins J Biol Chem 278 50 49721 31 doi 10 1074 jbc M306194200 PMID 14514668 Bieling P Laan L Schek H Munteanu EL Sandblad L Dogterom M Brunner D Surrey T 2007 Reconstitution of a microtubule plus end tracking system in vitro Nature 450 7172 1100 5 Bibcode 2007Natur 450 1100B doi 10 1038 nature06386 PMID 18059460 S2CID 4355669 Maurer SP Fourniol FJ Bohner G Moores CA Surrey T 2012 EBs recognize a nucleotide dependent structural cap at growing microtubule ends Cell 149 2 371 82 doi 10 1016 j cell 2012 02 049 PMC 3368265 PMID 22500803 Nakamura S Grigoriev I Nogi T Hamaji T Cassimeris L Mimori Kiyosue Y 2012 Dissecting the nanoscale distributions and functions of microtubule end binding proteins EB1 and ch TOG in interphase HeLa cells PLOS ONE 7 12 e51442 Bibcode 2012PLoSO 751442N doi 10 1371 journal pone 0051442 PMC 3520847 PMID 23251535 Zanic M Widlund PO Hyman AA Howard J 2013 Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels Nat Cell Biol 15 6 688 93 doi 10 1038 ncb2744 PMID 23666085 S2CID 3025200 Jie Chen 2014 Phosphoregulation of the dimerization and functions of end binding protein 1 Protein amp Cell 5 10 795 799 doi 10 1007 s13238 014 0081 9 PMC 4180461 PMID 25048701 nbsp This article on a gene on human chromosome 20 is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title MAPRE1 amp oldid 1217321415, wikipedia, wiki, book, books, library,
