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Wikipedia

List of restriction enzyme cutting sites: A

Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

This article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site.

The following information is given for each enzyme:

  • Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature,[1][2] and bibliographical references. Note: When alphabetizing, enzymes are first ordered alphabetically by the acronyms (everything before the roman numeral); then enzymes of a given acronym are ordered alphabetically by the roman numeral, treating the numeral as a number and not a string of letters. This helps keep the entries ordered hierarchically while also alphabetic.(Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.[3][4]
  • REBASE Number: Number used to identify restriction enzymes in the REBASE restriction enzyme database. This database includes important information about the enzyme such as Recognition sequence, source, and Isoschizomers, as well as other data, such as the commercial suppliers of the enzyme.
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Displays the cut site and pattern and products of the cut. The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site.[5][6]
  • Isoschizomers and neoschizomers: An isoschizomer is a restriction enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8–10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None as of [date]" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists, as in this not listed enzyme:  Abc123I 

Whole list navigation edit

Restriction enzymes edit

A edit

Enzyme PDB code REBASE Number Source Recognition sequence Cut Isoschizomers
AaaI 1 Acetobacter aceti 5' CGGCCG
3' GCCGGC
5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5'
BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII
AagI 4 Achromobacter agile 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI
AanI 15358 Arthrobacter aurescens RFL2 5' TTATAA
3' AATATT
5' ---TTA   TAA--- 3'
3' ---AAT   ATT--- 5'
PsiI, Csp1BORF7380P
AarI[7] 2892 Arthrobacter aurescens SS2-322 5' CACCTGC
3' GTGGACG
5' ---CACCTGCN3N   NNNN--- 3'
3' ---GTGGACGN3NNNNN   --- 5'
PaqCI
AasI 5465 Arthrobacter aurescens RFL3 5' GACN6GTC
3' CTGN6CAG
5' ---GACNNNN   NNGTC--- 3'
3' ---CTGNN   NNNNCAG--- 5'
DrdI, DseDI
AatI[8] 6 Acetobacter aceti 5' AGGCCT
3' TCCGGA
5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5'
AspMI, Eco147I,  Eco1524I,  PceI, Pme55I, SarI, Sru30DI, SteI
AatII[8][9][10][11] 7 Acetobacter aceti 5' GACGTC
3' CTGCAG
5' ---GACGT   C--- 3'
3' ---C   TGCAG--- 5'
Ssp5230I, ZraI
AauI 3010 Arthrobacter aurescens 5' TGTACA
3' ACATGT
5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5'
 BsmRI,  Bsp1407I, BsrGI, BstAUI, Ssp4800I, SspBI
AbaI 3029 Azospirillum brasilense UQ 1796 5' TGATCA
3' ACTAGT
5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5'
BclI, BsiQI, BspXII, BstT7I, FbaI, Ksp22I,  ParI 
AbeI 3097 Azotobacter beijerinckii Slo 54-028 5' CCTCAGC
3' GGAGTCG
5' ---CC   TCAGC--- 3'
3' ---GGAGT   CG--- 5'
BbvCI
AbrI 8 Azospirillum brasilense 5' CTCGAG
3' GAGCTC
5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5'
BluI, BspAAI, BssHI, EscI, PaeR7I, SciI, Sfr274I, Sol10179I, StrI, TliI
AbsI 14594 Arthrobacter sp. 7M06 5' CCTCGAGG
3' GGAGCTCC
5' ---CC   TCGAGG--- 3'
3' ---GGAGCT   CC--- 5'
None as of 4 Apr. 2019
AccI[12][13][14][15] 18 Acinetobacter calcoaceticus 5' GTMKAC
3' CAKMTG
5' ---GT   MKAC--- 3'
3' ---CAKM   TG--- 5'
FblI, XmiI
AccII[12][16] 19 Acinetobacter calcoaceticus 5' CGCG
3' GCGC
5' ---CG   CG--- 3'
3' ---GC   GC--- 5'
Bsh1236I, Bsp50I, BstFNI, BstUI, MvnI, SelI, BceBI, BepI, Bpu95I
AccIII[17] 20 Acinetobacter calcoaceticus 5' TCCGGA
3' AGGCCT
5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5'
Aor13HI, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI
Acc16I 2638 Acinetobacter calcoaceticus 16 5' TGCGCA
3' ACGCGT
5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5'
AosI, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
Acc36I[18] 4162 Acinetobacter calcoaceticus 36 5' ACCTGC
3' TGGACG
5' ---ACCTGCN3N   NNNN--- 3'
3' ---TGGACGN3NNNNN   --- 5'
BfuAI, BspMI, BveI
Acc65I[19] 14 Acinetobacter calcoaceticus 65 5' GGTACC
3' CCATGG
5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5'
AhaB8I, Asp718I, KpnI, SthI
Acc113I 2665 Acinetobacter calcoaceticus 113 5' AGTACT
3' TCATGA
5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5'
AssI,  BmcAI, Bpa34I,  DpaI, Eco255I, RflFII, ScaI, ZrmI
AccB1I 15 Acinetobacter calcoaceticus B1 5' GGYRCC
3' CCRYGG
5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5'
BanI, BbvBI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I
AccB2I 2666 Acinetobacter calcoaceticus B2 5' RGCGCY
3' YCGCGR
5' ---RGCGC   Y--- 3'
3' ---Y   CGCGR--- 5'
 BfoI,  Bme142I, Bsp143II, BstH2I, HaeII, LpnI
AccB7I[20] 16 Acinetobacter calcoaceticus B7 5' CCAN5TGG
3' GGTN5ACC
5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5'
AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I
AccBSI[21] 2733 Acinetobacter calcoaceticus BS 5' CCGCTC
3' GGCGAG
5' ---CCG   CTC--- 3'
3' ---GGC   GAG--- 5'
BsrBI, BstD102I, Bst31NI, MbiI
AccEBI 17 Acinetobacter calcoaceticus EBF 65/65 5' GGATCC
3' CCTAGG
5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
AsiI, BamHI, Bce751I, Bsp98I, BspAAIII, CelI, OkrAI, Uba4009I
AceI 2571 Anabaena cedrorum 5' GCWGC
3' CGWCG
5' ---G   CWGC--- 3'
3' ---CGWC   G--- 5'
ApeKI, SuiI, Taq52I, TseI
AceII 2570 Anabaena cedrorum 5' GCTAGC
3' CGATCG
5' ---GCTAG   C--- 3'
3' ---C   GATCG--- 5'
AsuNHI, BmtI,  BspOI, , LlaG2I, NheI, PstNHI
AceIII 2569 Anabaena cedrorum 5' CAGCTC
3' GTCGAG
5' ---CAGCTCN5N   NNNN--- 3'
3' ---GTCGAGN5NNNNN   --- 5'
— None as of May 2010 —
AciI[11][22] 21 Arthrobacter citreus 5' CCGC
3' GGCG
5' ---C   CGC--- 3'
3' ---GGC   G--- 5'
SsiI
AclI[23] 2181 Acinetobacter calcoaceticus M4 5' AACGTT
3' TTGCAA
5' ---AA   CGTT--- 3'
3' ---TTGC   AA--- 5'
Psp1406I
AclNI 2667 Acinetobacter calcoaceticus N20 5' ACTAGT
3' TGATCA
5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5'
AhlI, BcuI, SpeI
AclWI 2668 Acinetobacter calcoaceticus W2131 5' GGATC
3' CCTAG
5' ---GGATCN3N   N--- 3'
3' ---CCTAGN3NN   --- 5'
AlwI, BinI,  BpuFI, BsrWI,  BspPI, BstH9I,  Bst31TI,  EacI,  Ral8I 
AcoI 11822 Acinetobacter calcoaceticus 5' YGGCCR
3' RCCGGY
5' ---Y   GGCCR--- 3'
3' ---RCCGG   Y--- 5'
EaeI, Bfi89I, CfrI, EcoHK31I
AcpI 22 Acidiphilium cryptum 25H 5' TTCGAA
3' AAGCTT
5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5'
Asp10HI, Bpu14I, Bsp119I, BstBI, Csp45I, FspII, MlaI, NspV, SspRFI
AcpII 23 Acidiphilium cryptum 25H 5' CCAN5TGG
3' GGTN5ACC
5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5'
AccB7I, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I
AcrII 25 Anabaenopsis circularis 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AspAI, Bse64I, BseT9I, BstEII, BstPI, Eco91I, EcoO65I, PspEI
AcsI[24] 2184 Arthrobacter citreus 310 5' RAATTY
3' YTTAAR
5' ---R   AATTY--- 3'
3' ---YTTAA   R--- 5'
ApoI,  CfaI,  FsiI, XapI
AcuI 5534 Acinetobacter calcoaceticus 5' CTGAAG
3' GACTTC
5' ---CTGAAGN13NNN   --- 3'
3' ---GACTTCN13N   NN--- 5'
BspKT5I, Eco57I
AcvI 5359 Aeromonas caviae 5' CACGTG
3' GTGCAC
5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5'
BbrPI, BcoAI, Eco72I, PmaCI, PmlI, PspCI
AcyI[25] 31 Anabaena cylindrica 5' GRCGYC
3' CYGCRG
5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'
AhaII, AosII, BbiII, BsaHI, BstACI, Hin1I, HgiI, Hsp92I, Msp17I
AdeI 2991 Alcaligenes denitrificans Ss 3-028 5' CACNNNGTG
3' GTGNNNCAC
5' ---CACNNN   GTG--- 3'
3' ---GTG   NNNCAC--- 5'
BstIZ316I, DraIII
AeuI 33 Achromobacter eurydice 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AglI, AjnI, BseBI, BstNI, BstOI, Bst2UI, EcoRII, SleI, SspAI
AfaI[26] 37 Acidiphilium facilis 28H 5' GTAC
3' CATG
5' ---GT   AC--- 3'
3' ---CA   TG--- 5'
Csp6I, CviQI, CviRII, HpyBI, PabI, PlaAII, RsaI, RsaNI
Afa16RI 34 Acidiphilium sp. 16R 5' CGATCG
3' GCTAGC
5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5'
Afa22MI, BspCI, EagBI, MvrI, NblI, Ple19I, PvuI, Psu161I, RshI, XorII
Afa22MI 35 Acidocella facilis 5' CGATCG
3' GCTAGC
5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5'
Afa16RI, BspCI, EagBI, ErhB9I, NblI, Ple19I, Psu161I, PvuI, RshI
AfeI[27] 2669 Alcaligenes faecalis T2774 5' AGCGCT
3' TCGCGA
5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5'
AitI, Aor51H, Eco47III, FunI
AfiI 10489 Anoxybacillus flavithermus 5' CCN7GG
3' GGN7CC
5' ---CCNNNNN   NNGG--- 3'
3' ---GGNN   NNNNNCC--- 5'
Bsc4I, BseLI, BslI, BflI, Bsc107I, BsiYI, Bst22I
AflI[28] Anabaena flos-aquae 5' GGWCC
3' CCWGG
5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5'
Asp745I, BamNI, BcuAI, CauI, Csp68KI, DsaIV, Eco47I, SinI
AflII[28][29][30] 39 Anabaena flos-aquae 5' CTTAAG
3' GAATTC
5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5'
BfrI, BspTI, Bst98I, BstAFI, BstPZ740I, Esp4I, MspCI, Vha464I
AflIII[28][10] 40 Anabaena flos-aquae 5' ACRYGT
3' TGYRCA
5' ---A   CRYGT--- 3'
3' ---TGYRC   A--- 5'
 Asp90I 
AgeI[11][31] 42 Agrobacterium gelatinovorum 5' ACCGGT
3' TGGCCA
5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5'
AsiAI, AsiGI, BshTI, CsiAI, CspAI, PinAI
AglI[32] 6652 Arthrobacter globiformis 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AeuI, Bse16I, Bse17I, Bse24I, BshGI, EcoRII, SleI, SniI, SslI
AgsI Agrococcus sp. 25 5' TTSAA
3' AASTT
5' ---TTS   AA--- 3'
5' ---AA   STT--- 3'
None as of 4 May 2019
AhaI 45 Alteromonas haloplanktis B8 5' CCSGG
3' GGSCC
5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5'
AseII, BcnI,  BpuMI,  CauII, Eco1831I, EcoHI, HgiS22I, NciI
AhaII Aphanothece halophytica 5' GRCGYC
3' CYGCRG
5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'
AcyI, AstWI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiHII, PamII
AhaIII[14][29][30][33] Aphanothece halophytica 5' TTTAAA
3' AAATTT
5' ---TTT   AAA--- 3'
3' ---AAA   TTT--- 5'
DraI, PauAII, SruI
AhaB8I Aphanothece halophytica B8 5' GGTACC
3' CCATGG
5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5'
Acc65I, Asp718I, KpnI, SthI
AhdI[34][35] Aeromonas hydrophila 5' GACN5GTC
3' CTGN5CAG
5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5'
AspEI,  BmeRI,  BspOVI, DriI, Eam1105I, EclHKI, NruGI
AhlI 4838 Alteromonas haloplanktis SP 5' ACTAGT
3' TGATCA
5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5'
AclNI, BcuI, SpeI
AhyI 49 Aeromonas hydrophila 5' CCCGGG
3' GGGCCC
5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5'
Cfr9I, CfrJ4I, EaeAI, EclRI, PaeBI, SmaI, TspMI, XmaI, XmaCI
AitI 54 Aquaspirillum itersonii 5' AGCGCT
3' TCGCGA
5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5'
AfeI, Aor51H, Eco47III, FunI
AjiI 10919 Acinetobacter johnsonii RFL47 5' CACGTC
3' GTGCAG
5' ---CAC   GTC--- 3'
3' ---GTG   CAG--- 5'
BmgBI, BtrI
AjnI 7185 Acinetobacter johnsonii R2 5' CCWGG
3' GGWCC
5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5'
AorI, ApaORI, ApyI, EcoRII, MvaI, Psp6I, PspGI, SleI, SspAI
AjoI 2604 Acinetobacter johnsonii 35 5' CTGCAG
3' GACGTC
5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5'
ApiI, BloHII, BspMAI, CfrA4I, CfuII, PstI, SalPI, SflI, Srl5DI
AjuI 7740 Acinetobacter junii RFL46 5' GAAN7TTGG
3'CTTN7AACC
5' ---NNNNN   NN6GAAN7TTGGN5NNNNNN   --- 3'
3' ---   NNNNNNN6CTTN7AACCN5N   NNNNN--- 5'
None as of 28 Oct. 2020
AleI 5791 Aureobacterium liquefaciens 5' CACN4GTG
3' GTGN4CAC
5' ---CACNN   NNGTG--- 3'
3' ---GTGNN   NNCAC--- 5'
OliI
AlfI 6110 Acinetobacter lwoffii BH 32 5' GCAN6TGC
3' CGTN6ACG
5' ---GCAN6TGCN9NNN   --- 3'
3' ---CGTN6ACGN9N   NN--- 5'
None as of May 2010
AliI Acetobacter liquefaciens 5' GGATCC
3' CCTAGG
5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
AccEBI, BamHI, Bce751I, Bsp98I, BspAAIII, Nsp29132II, Pfl8I
AliAJI 59 Acetobacter liquefaciens AJ 2881 5' CTGCAG
3' GACGTC
5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5'
ApiI, Asp713I, BspMAI, CflI, CstI, MhaAI, PstI, SalPI, Sst12I, YenI
AloI[36][37] 2812 Acinetobacter lwoffii Ks 4-8 5' GAACN6TCC
3' CTTGN6AGG
5' ---GAACN6TCCN6NNNNNN   --- 3'
3' ---CTTGN6AGGN6N   NNNNN--- 5'
None as of May 2010
AluI[12][14][38][39][40][41] 61 Arthrobacter luteus 5' AGCT
3' TCGA
5' ---AG   CT--- 3'
3' ---TC   GA--- 5'
AluBI, MltI
AluBI 16189 Arthrobacter luteus B 5' AGCT
3' TCGA
5' ---AG   CT--- 3'
3' ---TC   GA--- 5'
AluI, MltI
AlwI 65 Acinetobacter lwoffii 5' GGATC
3' CCTAG
5' ---GGATCN3N   N--- 3'
3' ---CCTAGN3NN   --- 5'
AclWI, BinI, BspPI,  BsrWI,  BstH9I,  BthII, Bth617I,  EacI
Alw21I Acinetobacter lwoffii RFL21 5' GWGCWC
3' CWCGWG
5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5'
AspHI, Bbv12I, Bsh45I, BsiHKAI, Bsm6I,  HgiAI, MspV281I
Alw26I[42] 63 Acinetobacter lwoffii RFL26 5' GTCTC
3' CAGAG
5' ---GTCTCN   NNNN--- 3'
3' ---CAGAGNNNNN   --- 5'
 BcoDI, BscQII,   BsmAI, BsoMAI,  BstMAI 
Alw44I Acinetobacter lwoffii RFL44 5' GTGCAC
3' CACGTG
5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5'
ApaLI, SnoI, VneI
AlwNI[43] Acinetobacter lwoffii N 5' CAGNNNCTG
3' GTCNNNGAC
5' ---CAGNNN   CTG--- 3'
3' ---GTC   NNNGAC--- 5'
CaiI
AlwXI 67 Acinetobacter lwoffii X 5' GCAGC
3' CGTCG
5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5'
BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I
Ama87I Alteromonas macleodii 87 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
AquI, AvaI,  BmeT110I,  BsiHKCI, BsoBI, Eco88I, Nli3877I, NspSAI
AmaCSI 26714 Arthrospira maxima CS-328 5' GCTCCA
3' CGAGGT
5' ---GCTCCAN8NNN   --- 3'
3' ---CGAGGTN8N   NN--- 5'
None as of 4 May 2018
AocI 74 Anabaena sp. 5' CCTNAGG
3' GGANTCC
5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5'
AyI, Bse21I, Bsu36I, Eco81I, Lmu60I, OaNI, MstII, SauI, SshAI
AocII Anabaena sp. 5' GDGCHC
3' CHCGDG
5' ---GDGCH   C--- 3'
3' ---C   HCGDG--- 5'
BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII, SduI
AorI 77 Acetobacter aceti orleanensis 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
BseBI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, Sth117I, Tai
Aor13HI[44] Acidiphilium organovorum 13H 5' TCCGGA
3' AGGCCT
5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5'
AccIII, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI
Aor51HI[45] 76 Acidiphilium organovorum 51H 5' AGCGCT
3' TCGCGA
5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5'
AfeI, AitI, Eco47III, FunI
AosI Anabaena oscillarioides 5' TGCGCA
3' ACGCGT
5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5'
Acc16I, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
AosII Anabaena oscillarioides 5' GRCGYC
3' CYGCRG
5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'
AcyI, AstWI, BbiII, BsaHI, BstACI, Hin1I, HgiI, HgiGI, Msp17I, PamII
ApaI[11][30][46] Acetobacter pasteurianus pasteurianus 5' GGGCCC
3' CCCGGG
5' ---GGGCC   C--- 3'
3' ---C   CCGGG--- 5'
Bsp120I, PpeI, PspOMI
ApaBI[47] Acetobacter pasteurianus B 5' GCAN5TGC
3' CGTN5ACG
5' ---GCANNNNN   TGC--- 3'
3' ---CGT   NNNNNACG--- 5'
BstAPI
ApaCI Acetobacter pasteurianus C 5' GGATCC
3' CCTAGG
5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
AliI, BamHI, BnaI, Bsp4009I, BstI, NspSAIV, Pfl8I, SolI, SurI
ApaLI[48][49] Acetobacter pasteurianus 5' GTGCAC
3' CACGTG
5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5'
Alw44I, SnoI, VneI
ApaORI 86 Acetobacter pasteurianus 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AjnI, BstNI, BstOI, Bst1I, Bst2I, Fsp1604I, MvaI, Psp6I, PspGI
ApeKI[50] Aeropyrum pernix K1 5' GCWGC
3' CGWCG
5' ---G   CWGC--- 3'
3' ---CGWC   G--- 5'
AceI, SuiI, Taq52I, TseI
ApiI 2315 Arthrobacter picolinophilus 5' CTGCAG
3' GACGTC
5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5'
Asp713I, Bsp63I, CfrA4I, PaePI, Pfl21I, Psp23I, PstI, SflI, Sst12I
ApoI[51] Arthrobacter protophormiae 5' RAATTY
3' YTTAAR
5' ---R   AATTY--- 3'
3' ---YTTAA   R--- 5'
AcsI, CfaI,  FsiI, XapI, EcoRI
AplI 25962 Arthrospira platensis NIES-39 5' CTGCAG
3' GACGTC
5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5'
See this site for a complete list.
ApyI 93 Arthrobacter pyridinolis 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
BciBII, BptI, BseBI, BstNI, BstOI, Bst2UI, Bst38I, Bst100I, MvaI
ApyPI 14756 Arcanobacterium pyogenes 5' ATCGAC
3' TAGCTG
5' ---ATCGACN17NNN   --- 3'
3' ---TAGCTGN17N   NN--- 5'
None as of 4 August 2019
AquI Agmenellum quadruplicatum PR-6 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
BcoI,  BmeT110I,  BsmAI, BsoMAI, BsoBI, BstSI, Eco27kI, OfoI
AquII 20238 Agmenellum quadruplicatum PR-6 5' GCCGNAC
3' CGGCNTG
5' ---GCCGNACN17NNN   --- 3'
3' ---CGGCNTGN17N   NN--- 5'
None as of 4 August 2019
AquIII 20239 Agmenellum quadruplicatum PR-6 5' GAGGAG
3' CTCCTC
5' ---GAGGAGN17NNN   --- 3'
3' ---CTCCTCN17N   NN--- 5'
BseRI
AquIV 20240 Agmenellum quadruplicatum PR-6 5' GRGGAAG
3' CYCCTTC
5' ---GRGGAAGN16NNN   --- 3'
3' ---CYCCTTCN16N   NN--- 5'
None as of 4/9/19
ArsI 19405 Arthrobacter sp. NTS 5' GACN6TTYG
3' CTGN6AARC
5' ---NNNNN   NN7GACN6TTYGN5NNNNNN   --- 3'
3' ---   NNNNNNN7CTGN6AARCN5N   NNNNN--- 5'
None as of Oct. 28 2020
AscI[11][52] Arthrobacter sp. 5' GGCGCGCC
3' CCGCGCGG
5' ---GG   CGCGCC--- 3'
3' ---CCGCGC   GG--- 5'
 PalAI, SgsI 
AseI[53] 96 Aquaspirillum serpens 5' ATTAAT
3' TAATTA
5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5'
AsnI, BpoAI, PshBI, Sru4DI, VspI
AseII[53] 97 Aquaspirillum serpens 5' CCSGG
3' GGSCC
5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5'
AhaI, AsuC2I, BcnI, CauII, EcoHI, HgiS22I, Kpn49kII, Mgl14481I, NciI
AsiI Azotobacter sp. 5' GGATCC
3' CCTAGG
5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
ApaCI, BamHI, BnaI, Bsp4009I, BstI, OkrAI, Pfl8I, SurI, Uba4009I
AsiAI 2853 Arthrobacter sp. A7359 5' ACCGGT
3' TGGCCA
5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5'
AgeI, AsiGI, BshTI, CsiAI, CspAI, PinAI
AsiGI 10828 Arthrobacter sp. G 5' ACCGGT
3' TGGCCA
5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5'
AgeI, AsiAI, BshTI, CsiAI, CspAI, PinAI
AsiSI Arthrobacter sp. 5' GCGATCGC
3' CGCTAGCG
5' ---GCGAT   CGC--- 3'
3' ---CGC   TAGCG--- 5'
 RgaI, SfaAI, , SgfI
Asi256I 18472 Arthrobacter sp. Ck256 5' GATC
3' CTAG
5' ---G   ATC--- 3'
3' ---CTA   G--- 5'
See this site for a complete list.
Asi372I 15803 Arthrobacter sp. Mn372 5' ATGCAT
3' TACGTA
5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5'
Ppu10I, EcoT22I, Mph1103I, NsiI, Zsp2I, BfrBI, Csp68KIII, PinBI, SepI, SspD5II
AsnI[54] 98 Arthrobacter sp. N-CM 5' ATTAAT
3' TAATTA
5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5'
AseI, BpoAI, PshBI, Sru4DI, VspI
AspI[55] Achromobacter sp. 699 5' GACNNNGTC
3' CTGNNNCAG
5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5'
AtsI, PflFI, PsyI, TelI, Tth111I
Asp10HI Acidiphilium sp. 10H 5' TTCGAA
3' AAGCTT
5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5'
AsuII, BspT104I, BstBI, CbiI, Csp68KII, PlaII, PpaAI, Ssp1I
Asp10HII 100 Acidiphilium sp. 10H 5' CCAN5TGG
3' GGTN5ACC
5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5'
AccB7I, AcpII, BasI, Esp1396I, PflBI, PflMI, Van91I
Asp26HI 111 Acidiphilium sp. 26H 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, PctI
Asp27HI 112 Acidiphilium sp. 27H 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp35HI, Asp36HI, Asp40HI,  BmaHI,  BsaMI, BscCI, BsmI
Asp35HI 117 Acidiphilium sp. 35H 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp36HI, Asp40HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I
Asp36HI 118 Acidiphilium sp. 36H 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp40HI, Asp50HI,  BmaHI,  BscCI, BsmI, Mva1269I, PctI
Asp40HI 120 Acidiphilium sp. 40H 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp27HI, Asp36HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I
Asp50HI 122 Acidiphilium sp. 50H 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp26HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, Mva1269I
Asp700I[56] Achromobacter sp. 700 5' GAAN4TTC
3' CTTN4AAG
5' ---GAANN   NNTTC--- 3'
3' ---CTTNN   NNAAG--- 5'
BbvAI, MroXI, PdmI, XmnI
Asp713I 131 Achromobacter sp. 713 5' CTGCAG
3' GACGTC
5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5'
AjoI, AliAJI, BloHII, CfrA4I, Ecl37kI, MhaAI, PstI, SflI, Srl5DI, YenI
Asp718I[57] Achromobacter sp. 718 5' GGTACC
3' CCATGG
5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5'
Acc65I, AhaB8I, KpnI, SthI
Asp745I Achromobacter sp. 745 5' GGWCC
3' CCWGG
5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5'
Bme18I, BsrAI, CauI, Eco47I, ErpI, FdiI, SinI, VpaK11AI, VpaK11BI
AspAI Alcaligenes sp. 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AcrII, Bse64I, BseT10I, BstEII, BstPI, Eco91I, EcoO65I, PspEI
AspA2I 6959 Arthrobacter sp. A2 5' CCTAGG
3' GGATCC
5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5'
AvrBII, AvrII, BlnI, BspA2I, XmaJI
AspEI[58] Aureobacterium sp. 5' GACN5GTC
3' CTGN5CAG
5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5'
AhdI,  BmeRI,  BspOVI, DriI, Eam1105I, EclHKI, NruGI
AspHI[59] Achromobacter sp. H 5' GWGCWC
3' CWCGWG
5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5'
Alw21I, Bbv12I, Bsh45I, BsiHKAI,  Bsm6I,  HgiAI, MspV281I
AspLEI Arthrobacter sp. LE3860 5' GCGC
3' CGCG
5' ---GCG   C--- 3'
3' ---C   GCG--- 5'
BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI
AspMI 2977 Acinetobacter sp. M 5' AGGCCT
3' TCCGGA
5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5'
AatI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuI
AspMDI Alcaligenes sp. MD1 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
BfuCI, Bsp143I, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
AspNI Anabaena sp. J3 5' GGNNCC
3' CCNNGG
5' ---GGN   NCC--- 3'
3' ---CCN   NGG--- 5'
 BmiI,  BscBI, BspLI, NlaIV, PspN4I
AspS9I Arthrobacter sp. S9 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AsuI, AvcI, Bac36I, Bal228I, FmuI, NspIV, PspPI, Sau96I
AssI 4930 Arthrobacter sp. 5' AGTACT
3' TCATGA
5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5'
Acc113I,  BmcAI, Bpa34I,  DpaI, Eco255I, RflFII, ScaI, ZrmI
AstWI Anabaena sp. 5' GRCGYC
3' CYGCRG
5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'
AcyI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiGI, Msp17I
AsuI[60] Anabaena subcylindrica 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AspS9I, AvcI, CcuI, Cfr13I, FmuI, MaeK81II, Nsp7121I, PspPI, UnbI
AsuII[61] Anabaena subcylindrica 5' TTCGAA
3' AAGCTT
5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5'
AcpI, Bpu14I, Bsp119I, BspT104I, Csp45I, FspII, LspI, NspV, SfuI
AsuIII Anabaena subcylindrica 5' GRCGYC
3' CYGCRG
5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'
AcyI, AsuIII, BbiII, BsaHI, BstACI, Hin1I, HgiHII, Hsp92I, Msp17I
AsuC2I 2901 Actinobacillus suis C2 5' CCSGG
3' GGSCC
5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5'
AhaI, AseII, BcnI, Eco1831I, EcoHI, Kpn49kII, Mgl14481I, NciI
AsuHPI[62] 2811 Actinobacillus suis HP 5' GGTGA
3' CCACT
5' ---GGTGAN6NN   --- 3'
3' ---CCACTN6N   N--- 5'
HphI, SspD5I
AsuNHI[62] Actinobacillus suis NH 5' GCTAGC
3' CGATCG
5' ---G   CTAGC--- 3'
3' ---CGATC   G--- 5'
AceII, BmtI,  BspOI,  LlaG2I, NheI, PstNHI
AtsI Aureobacterium testaceum 4842 5' GACNNNGTC
3' CTGNNNCAG
5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5'
AspI, PflFI, PsyI, TelI, Tth111I
AvaI[14][63][64] Anabaena variabilis 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
AquI, Ama87I, BsiHKCI, BsoBI, BspLU4I, Eco88I, NspIII, PlaAI
AvaII[12][14][41][63][64] Anabaena variabilis 5' GGWCC
3' CCWGG
5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5'
Bme18I, Bme216I, BsrAI, Eco47I, Kzo49I, Psp03I, SmuEI, VpaK11AI
AvcI Actinomyces violaceoniger cristalomycini 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AspS9I, BshKI, BsiZI, Bsp1894I, BspBII, BspF4I, Bsu54I, Pde12I
AviII Anabaena variabillis (halle) 5' TGCGCA
3' ACGCGT
5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5'
Acc16I, AosI, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
AvrII[11][29] 173 Anabaena variabilis UW 5' CCTAGG
3' GGATCC
5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5'
AspA2I, AvrBII, BlnI, BspA2I, XmaJI
AvrBII 171 Arthrobacter variabilis 5' CCTAGG
3' GGATCC
5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5'
AspA2I, AvrII, BlnI, BspA2I, XmaJI
AxyI[65] 174 Acetobacter xylinus 5' CCTNAGG
3' GGANTCC
5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5'
AocI, BliHKI, Bse21I, BspR7I, Bsu36I, CvnI, Eco81I, Lmu60I

§ An HF version of this enzyme is available

Notes edit

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list, restriction, enzyme, cutting, sites, main, articles, list, restriction, enzyme, cutting, sites, restriction, enzyme, legend, nucleobasescode, nucleotide, representeda, adenine, cytosine, guanine, thymine, tthis, article, contains, list, restriction, enzy. Main articles List of restriction enzyme cutting sites and Restriction enzyme Legend of nucleobasesCode Nucleotide representedA Adenine A C Cytosine C G Guanine G T Thymine T N A C G or TM A or CR A or GW A or TY C or TS C or GK G or TH A C or TB C G or TV A C or GD A G or TThis article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site The following information is given for each enzyme Name of Restriction Enzyme Accepted name of the molecule according to the internationally adopted nomenclature 1 2 and bibliographical references Note When alphabetizing enzymes are first ordered alphabetically by the acronyms everything before the roman numeral then enzymes of a given acronym are ordered alphabetically by the roman numeral treating the numeral as a number and not a string of letters This helps keep the entries ordered hierarchically while also alphabetic Further reading see the section Nomenclature in the article Restriction enzyme PDB code Code used to identify the structure of a protein in the PDB database of protein structures The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action 3 4 REBASE Number Number used to identify restriction enzymes in the REBASE restriction enzyme database This database includes important information about the enzyme such as Recognition sequence source and Isoschizomers as well as other data such as the commercial suppliers of the enzyme Source Organism that naturally produces the enzyme Recognition sequence Sequence of DNA recognized by the enzyme and to which it specifically binds Cut Displays the cut site and pattern and products of the cut The recognition sequence and the cut site usually match but sometimes the cut site can be dozens of nucleotides away from the recognition site 5 6 Isoschizomers and neoschizomers An isoschizomer is a restriction enzyme that recognizes the same sequence as another A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another but cuts in a different manner A maximum number of 8 10 most common isoschizomers are indicated for every enzyme but there may be many more Neoschizomers are shown in bold and green color font e g BamHI When None as of date is indicated that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists as in this not listed enzyme Abc123I Whole list navigation editMain page A Ba Bc Bd Bp Bsa Bso Bsp Bsr Bss Bst Bsu Bv C D E F G H I K L M N O P R S T U V X Y Z External links See alsoRestriction enzymes editA edit Enzyme PDB code REBASE Number Source Recognition sequence Cut IsoschizomersAaaI 1 Acetobacter aceti 5 CGGCCG 3 GCCGGC 5 C span style padding left 2em span GGCCG 3 3 GCCGG span style padding left 2em span C 5 BseX3I BstZI EagI EclXI Eco52I SenPT16I XmaIIIAagI 4 Achromobacter agile 5 ATCGAT 3 TAGCTA 5 AT span style padding left 2em span CGAT 3 3 TAGC span style padding left 2em span TA 5 BanIII BavCI Bsa29I BseCI BspDI Bsu15I BsuTUI ClaIAanI 15358 Arthrobacter aurescens RFL2 5 TTATAA 3 AATATT 5 TTA span style padding left 2em span TAA 3 3 AAT span style padding left 2em span ATT 5 PsiI Csp1BORF7380PAarI 7 2892 Arthrobacter aurescens SS2 322 5 CACCTGC 3 GTGGACG 5 CACCTGCN sub 3 sub N span style padding left 2em span NNNN 3 3 GTGGACGN sub 3 sub NNNNN span style padding left 2em span 5 PaqCIAasI 5465 Arthrobacter aurescens RFL3 5 GACN sub 6 sub GTC 3 CTGN sub 6 sub CAG 5 GACNNNN span style padding left 2em span NNGTC 3 3 CTGNN span style padding left 2em span NNNNCAG 5 DrdI DseDIAatI 8 6 Acetobacter aceti 5 AGGCCT 3 TCCGGA 5 AGG span style padding left 2em span CCT 3 3 TCC span style padding left 2em span GGA 5 AspMI Eco147I Eco1524I PceI Pme55I SarI Sru30DI SteIAatII 8 9 10 11 7 Acetobacter aceti 5 GACGTC 3 CTGCAG 5 GACGT span style padding left 2em span C 3 3 C span style padding left 2em span TGCAG 5 Ssp5230I ZraIAauI 3010 Arthrobacter aurescens 5 TGTACA 3 ACATGT 5 T span style padding left 2em span GTACA 3 3 ACATG span style padding left 2em span T 5 BsmRI Bsp1407I BsrGI BstAUI Ssp4800I SspBIAbaI 3029 Azospirillum brasilense UQ 1796 5 TGATCA 3 ACTAGT 5 T span style padding left 2em span GATCA 3 3 ACTAG span style padding left 2em span T 5 BclI BsiQI BspXII BstT7I FbaI Ksp22I ParI AbeI 3097 Azotobacter beijerinckii Slo 54 028 5 CCTCAGC 3 GGAGTCG 5 CC span style padding left 2em span TCAGC 3 3 GGAGT span style padding left 2em span CG 5 BbvCIAbrI 8 Azospirillum brasilense 5 CTCGAG 3 GAGCTC 5 C span style padding left 2em span TCGAG 3 3 GAGCT span style padding left 2em span C 5 BluI BspAAI BssHI EscI PaeR7I SciI Sfr274I Sol10179I StrI TliIAbsI 14594 Arthrobacter sp 7M06 5 CCTCGAGG 3 GGAGCTCC 5 CC span style padding left 2em span TCGAGG 3 3 GGAGCT span style padding left 2em span CC 5 None as of 4 Apr 2019AccI 12 13 14 15 18 Acinetobacter calcoaceticus 5 GTMKAC 3 CAKMTG 5 GT span style padding left 2em span MKAC 3 3 CAKM span style padding left 2em span TG 5 FblI XmiIAccII 12 16 19 Acinetobacter calcoaceticus 5 CGCG 3 GCGC 5 CG span style padding left 2em span CG 3 3 GC span style padding left 2em span GC 5 Bsh1236I Bsp50I BstFNI BstUI MvnI SelI BceBI BepI Bpu95IAccIII 17 20 Acinetobacter calcoaceticus 5 TCCGGA 3 AGGCCT 5 T span style padding left 2em span CCGGA 3 3 AGGCC span style padding left 2em span T 5 Aor13HI BbvAIII BlfI BseAI BsiMI BspEI CauB3I Kpn2I MroI PtaIAcc16I 2638 Acinetobacter calcoaceticus 16 5 TGCGCA 3 ACGCGT 5 TGC span style padding left 2em span GCA 3 3 ACG span style padding left 2em span CGT 5 AosI AviII FdiII FspI MstI NsbI PamI Pun14627IAcc36I 18 4162 Acinetobacter calcoaceticus 36 5 ACCTGC 3 TGGACG 5 ACCTGCN sub 3 sub N span style padding left 2em span NNNN 3 3 TGGACGN sub 3 sub NNNNN span style padding left 2em span 5 BfuAI BspMI BveIAcc65I 19 14 Acinetobacter calcoaceticus 65 5 GGTACC 3 CCATGG 5 G span style padding left 2em span GTACC 3 3 CCATG span style padding left 2em span G 5 AhaB8I Asp718I KpnI SthIAcc113I 2665 Acinetobacter calcoaceticus 113 5 AGTACT 3 TCATGA 5 AGT span style padding left 2em span ACT 3 3 TCA span style padding left 2em span TGA 5 AssI BmcAI Bpa34I DpaI Eco255I RflFII ScaI ZrmIAccB1I 15 Acinetobacter calcoaceticus B1 5 GGYRCC 3 CCRYGG 5 G span style padding left 2em span GYRCC 3 3 CCRYG span style padding left 2em span G 5 BanI BbvBI BshNI BspT107I Eco64I HgiCI HgiHI MspB4IAccB2I 2666 Acinetobacter calcoaceticus B2 5 RGCGCY 3 YCGCGR 5 RGCGC span style padding left 2em span Y 3 3 Y span style padding left 2em span CGCGR 5 BfoI Bme142I Bsp143II BstH2I HaeII LpnIAccB7I 20 16 Acinetobacter calcoaceticus B7 5 CCAN sub 5 sub TGG 3 GGTN sub 5 sub ACC 5 CCANNNN span style padding left 2em span NTGG 3 3 GGTN span style padding left 2em span NNNNACC 5 AcpII Asp10HII BasI Esp1396I PflBI PflMI Van91IAccBSI 21 2733 Acinetobacter calcoaceticus BS 5 CCGCTC 3 GGCGAG 5 CCG span style padding left 2em span CTC 3 3 GGC span style padding left 2em span GAG 5 BsrBI BstD102I Bst31NI MbiIAccEBI 17 Acinetobacter calcoaceticus EBF 65 65 5 GGATCC 3 CCTAGG 5 G span style padding left 2em span GATCC 3 3 CCTAG span style padding left 2em span G 5 AsiI BamHI Bce751I Bsp98I BspAAIII CelI OkrAI Uba4009IAceI 2571 Anabaena cedrorum 5 GCWGC 3 CGWCG 5 G span style padding left 2em span CWGC 3 3 CGWC span style padding left 2em span G 5 ApeKI SuiI Taq52I TseIAceII 2570 Anabaena cedrorum 5 GCTAGC 3 CGATCG 5 GCTAG span style padding left 2em span C 3 3 C span style padding left 2em span GATCG 5 AsuNHI BmtI BspOI LlaG2I NheI PstNHIAceIII 2569 Anabaena cedrorum 5 CAGCTC 3 GTCGAG 5 CAGCTCN sub 5 sub N span style padding left 2em span NNNN 3 3 GTCGAGN sub 5 sub NNNNN span style padding left 2em span 5 None as of May 2010 AciI 11 22 21 Arthrobacter citreus 5 CCGC 3 GGCG 5 C span style padding left 2em span CGC 3 3 GGC span style padding left 2em span G 5 SsiIAclI 23 2181 Acinetobacter calcoaceticus M4 5 AACGTT 3 TTGCAA 5 AA span style padding left 2em span CGTT 3 3 TTGC span style padding left 2em span AA 5 Psp1406IAclNI 2667 Acinetobacter calcoaceticus N20 5 ACTAGT 3 TGATCA 5 A span style padding left 2em span CTAGT 3 3 TGATC span style padding left 2em span A 5 AhlI BcuI SpeIAclWI 2668 Acinetobacter calcoaceticus W2131 5 GGATC 3 CCTAG 5 GGATCN sub 3 sub N span style padding left 2em span N 3 3 CCTAGN sub 3 sub NN span style padding left 2em span 5 AlwI BinI BpuFI BsrWI BspPI BstH9I Bst31TI EacI Ral8I AcoI 11822 Acinetobacter calcoaceticus 5 YGGCCR 3 RCCGGY 5 Y span style padding left 2em span GGCCR 3 3 RCCGG span style padding left 2em span Y 5 EaeI Bfi89I CfrI EcoHK31IAcpI 22 Acidiphilium cryptum 25H 5 TTCGAA 3 AAGCTT 5 TT span style padding left 2em span CGAA 3 3 AAGC span style padding left 2em span TT 5 Asp10HI Bpu14I Bsp119I BstBI Csp45I FspII MlaI NspV SspRFIAcpII 23 Acidiphilium cryptum 25H 5 CCAN sub 5 sub TGG 3 GGTN sub 5 sub ACC 5 CCANNNN span style padding left 2em span NTGG 3 3 GGTN span style padding left 2em span NNNNACC 5 AccB7I Asp10HII BasI Esp1396I PflBI PflMI Van91IAcrII 25 Anabaenopsis circularis 5 GGTNACC 3 CCANTGG 5 G span style padding left 2em span GTNACC 3 3 CCANTG span style padding left 2em span G 5 AspAI Bse64I BseT9I BstEII BstPI Eco91I EcoO65I PspEIAcsI 24 2184 Arthrobacter citreus 310 5 RAATTY 3 YTTAAR 5 R span style padding left 2em span AATTY 3 3 YTTAA span style padding left 2em span R 5 ApoI CfaI FsiI XapIAcuI 5534 Acinetobacter calcoaceticus 5 CTGAAG 3 GACTTC 5 CTGAAGN sub 13 sub NNN span style padding left 2em span 3 3 GACTTCN sub 13 sub N span style padding left 2em span NN 5 BspKT5I Eco57IAcvI 5359 Aeromonas caviae 5 CACGTG 3 GTGCAC 5 CAC span style padding left 2em span GTG 3 3 GTG span style padding left 2em span CAC 5 BbrPI BcoAI Eco72I PmaCI PmlI PspCIAcyI 25 31 Anabaena cylindrica 5 GRCGYC 3 CYGCRG 5 GR span style padding left 2em span CGYC 3 3 CYGC span style padding left 2em span RG 5 AhaII AosII BbiII BsaHI BstACI Hin1I HgiI Hsp92I Msp17IAdeI 2991 Alcaligenes denitrificans Ss 3 028 5 CACNNNGTG 3 GTGNNNCAC 5 CACNNN span style padding left 2em span GTG 3 3 GTG span style padding left 2em span NNNCAC 5 BstIZ316I DraIIIAeuI 33 Achromobacter eurydice 5 CCWGG 3 GGWCC 5 CC span style padding left 2em span WGG 3 3 GGW span style padding left 2em span CC 5 AglI AjnI BseBI BstNI BstOI Bst2UI EcoRII SleI SspAIAfaI 26 37 Acidiphilium facilis 28H 5 GTAC 3 CATG 5 GT span style padding left 2em span AC 3 3 CA span style padding left 2em span TG 5 Csp6I CviQI CviRII HpyBI PabI PlaAII RsaI RsaNIAfa16RI 34 Acidiphilium sp 16R 5 CGATCG 3 GCTAGC 5 CGAT span style padding left 2em span CG 3 3 GC span style padding left 2em span TAGC 5 Afa22MI BspCI EagBI MvrI NblI Ple19I PvuI Psu161I RshI XorIIAfa22MI 35 Acidocella facilis 5 CGATCG 3 GCTAGC 5 CGAT span style padding left 2em span CG 3 3 GC span style padding left 2em span TAGC 5 Afa16RI BspCI EagBI ErhB9I NblI Ple19I Psu161I PvuI RshIAfeI 27 2669 Alcaligenes faecalis T2774 5 AGCGCT 3 TCGCGA 5 AGC span style padding left 2em span GCT 3 3 TCG span style padding left 2em span CGA 5 AitI Aor51H Eco47III FunIAfiI 10489 Anoxybacillus flavithermus 5 CCN sub 7 sub GG 3 GGN sub 7 sub CC 5 CCNNNNN span style padding left 2em span NNGG 3 3 GGNN span style padding left 2em span NNNNNCC 5 Bsc4I BseLI BslI BflI Bsc107I BsiYI Bst22IAflI 28 Anabaena flos aquae 5 GGWCC 3 CCWGG 5 G span style padding left 2em span GWCC 3 3 CCWG span style padding left 2em span G 5 Asp745I BamNI BcuAI CauI Csp68KI DsaIV Eco47I SinIAflII 28 29 30 39 Anabaena flos aquae 5 CTTAAG 3 GAATTC 5 C span style padding left 2em span TTAAG 3 3 GAATT span style padding left 2em span C 5 BfrI BspTI Bst98I BstAFI BstPZ740I Esp4I MspCI Vha464IAflIII 28 10 40 Anabaena flos aquae 5 ACRYGT 3 TGYRCA 5 A span style padding left 2em span CRYGT 3 3 TGYRC span style padding left 2em span A 5 Asp90I AgeI 11 31 42 Agrobacterium gelatinovorum 5 ACCGGT 3 TGGCCA 5 A span style padding left 2em span CCGGT 3 3 TGGCC span style padding left 2em span A 5 AsiAI AsiGI BshTI CsiAI CspAI PinAIAglI 32 6652 Arthrobacter globiformis 5 CCWGG 3 GGWCC 5 CC span style padding left 2em span WGG 3 3 GGW span style padding left 2em span CC 5 AeuI Bse16I Bse17I Bse24I BshGI EcoRII SleI SniI SslIAgsI Agrococcus sp 25 5 TTSAA 3 AASTT 5 TTS span style padding left 2em span AA 3 5 AA span style padding left 2em span STT 3 None as of 4 May 2019AhaI 45 Alteromonas haloplanktis B8 5 CCSGG 3 GGSCC 5 CC span style padding left 2em span SGG 3 3 GGS span style padding left 2em span CC 5 AseII BcnI BpuMI CauII Eco1831I EcoHI HgiS22I NciIAhaII Aphanothece halophytica 5 GRCGYC 3 CYGCRG 5 GR span style padding left 2em span CGYC 3 3 CYGC span style padding left 2em span RG 5 AcyI AstWI AsuIII BsaHI BstACI Hin1I HgiI HgiDI HgiHII PamIIAhaIII 14 29 30 33 Aphanothece halophytica 5 TTTAAA 3 AAATTT 5 TTT span style padding left 2em span AAA 3 3 AAA span style padding left 2em span TTT 5 DraI PauAII SruIAhaB8I Aphanothece halophytica B8 5 GGTACC 3 CCATGG 5 G span style padding left 2em span GTACC 3 3 CCATG span style padding left 2em span G 5 Acc65I Asp718I KpnI SthIAhdI 34 35 Aeromonas hydrophila 5 GACN sub 5 sub GTC 3 CTGN sub 5 sub CAG 5 GACNNN span style padding left 2em span NNGTC 3 3 CTGNN span style padding left 2em span NNNCAG 5 AspEI BmeRI BspOVI DriI Eam1105I EclHKI NruGIAhlI 4838 Alteromonas haloplanktis SP 5 ACTAGT 3 TGATCA 5 A span style padding left 2em span CTAGT 3 3 TGATC span style padding left 2em span A 5 AclNI BcuI SpeIAhyI 49 Aeromonas hydrophila 5 CCCGGG 3 GGGCCC 5 C span style padding left 2em span CCGGG 3 3 GGGCC span style padding left 2em span C 5 Cfr9I CfrJ4I EaeAI EclRI PaeBI SmaI TspMI XmaI XmaCIAitI 54 Aquaspirillum itersonii 5 AGCGCT 3 TCGCGA 5 AGC span style padding left 2em span GCT 3 3 TCG span style padding left 2em span CGA 5 AfeI Aor51H Eco47III FunIAjiI 10919 Acinetobacter johnsonii RFL47 5 CACGTC 3 GTGCAG 5 CAC span style padding left 2em span GTC 3 3 GTG span style padding left 2em span CAG 5 BmgBI BtrIAjnI 7185 Acinetobacter johnsonii R2 5 CCWGG 3 GGWCC 5 span style padding left 2em span CCWGG 3 3 GGWCC span style padding left 2em span 5 AorI ApaORI ApyI EcoRII MvaI Psp6I PspGI SleI SspAIAjoI 2604 Acinetobacter johnsonii 35 5 CTGCAG 3 GACGTC 5 CTGCA span style padding left 2em span G 3 3 G span style padding left 2em span ACGTC 5 ApiI BloHII BspMAI CfrA4I CfuII PstI SalPI SflI Srl5DIAjuI 7740 Acinetobacter junii RFL46 5 GAAN sub 7 sub TTGG 3 CTTN sub 7 sub AACC 5 NNNNN span style padding left 2em span NN sub 6 sub GAAN sub 7 sub TTGGN sub 5 sub NNNNNN span style padding left 2em span 3 3 span style padding left 2em span NNNNNNN sub 6 sub CTTN sub 7 sub AACCN sub 5 sub N span style padding left 2em span NNNNN 5 None as of 28 Oct 2020AleI 5791 Aureobacterium liquefaciens 5 CACN sub 4 sub GTG 3 GTGN sub 4 sub CAC 5 CACNN span style padding left 2em span NNGTG 3 3 GTGNN span style padding left 2em span NNCAC 5 OliIAlfI 6110 Acinetobacter lwoffii BH 32 5 GCAN sub 6 sub TGC 3 CGTN sub 6 sub ACG 5 GCAN sub 6 sub TGCN sub 9 sub NNN span style padding left 2em span 3 3 CGTN sub 6 sub ACGN sub 9 sub N span style padding left 2em span NN 5 None as of May 2010AliI Acetobacter liquefaciens 5 GGATCC 3 CCTAGG 5 G span style padding left 2em span GATCC 3 3 CCTAG span style padding left 2em span G 5 AccEBI BamHI Bce751I Bsp98I BspAAIII Nsp29132II Pfl8IAliAJI 59 Acetobacter liquefaciens AJ 2881 5 CTGCAG 3 GACGTC 5 CTGCA span style padding left 2em span G 3 3 G span style padding left 2em span ACGTC 5 ApiI Asp713I BspMAI CflI CstI MhaAI PstI SalPI Sst12I YenIAloI 36 37 2812 Acinetobacter lwoffii Ks 4 8 5 GAACN sub 6 sub TCC 3 CTTGN sub 6 sub AGG 5 GAACN sub 6 sub TCCN sub 6 sub NNNNNN span style padding left 2em span 3 3 CTTGN sub 6 sub AGGN sub 6 sub N span style padding left 2em span NNNNN 5 None as of May 2010AluI 12 14 38 39 40 41 61 Arthrobacter luteus 5 AGCT 3 TCGA 5 AG span style padding left 2em span CT 3 3 TC span style padding left 2em span GA 5 AluBI MltIAluBI 16189 Arthrobacter luteus B 5 AGCT 3 TCGA 5 AG span style padding left 2em span CT 3 3 TC span style padding left 2em span GA 5 AluI MltIAlwI 65 Acinetobacter lwoffii 5 GGATC 3 CCTAG 5 GGATCN sub 3 sub N span style padding left 2em span N 3 3 CCTAGN sub 3 sub NN span style padding left 2em span 5 AclWI BinI BspPI BsrWI BstH9I BthII Bth617I EacIAlw21I Acinetobacter lwoffii RFL21 5 GWGCWC 3 CWCGWG 5 GWGCW span style padding left 2em span C 3 3 C span style padding left 2em span WCGWG 5 AspHI Bbv12I Bsh45I BsiHKAI Bsm6I HgiAI MspV281IAlw26I 42 63 Acinetobacter lwoffii RFL26 5 GTCTC 3 CAGAG 5 GTCTCN span style padding left 2em span NNNN 3 3 CAGAGNNNNN span style padding left 2em span 5 BcoDI BscQII BsmAI BsoMAI BstMAI Alw44I Acinetobacter lwoffii RFL44 5 GTGCAC 3 CACGTG 5 G span style padding left 2em span TGCAC 3 3 CACGT span style padding left 2em span G 5 ApaLI SnoI VneIAlwNI 43 Acinetobacter lwoffii N 5 CAGNNNCTG 3 GTCNNNGAC 5 CAGNNN span style padding left 2em span CTG 3 3 GTC span style padding left 2em span NNNGAC 5 CaiIAlwXI 67 Acinetobacter lwoffii X 5 GCAGC 3 CGTCG 5 GCAGCN sub 7 sub N span style padding left 2em span NNNN 3 3 CGTCGN sub 7 sub NNNNN span style padding left 2em span 5 BbvI BseKI BseXI Bsp423I Bst12I Bst71I BstV1IAma87I Alteromonas macleodii 87 5 CYCGRG 3 GRGCYC 5 C span style padding left 2em span YCGRG 3 3 GRGCY span style padding left 2em span C 5 AquI AvaI BmeT110I BsiHKCI BsoBI Eco88I Nli3877I NspSAIAmaCSI 26714 Arthrospira maxima CS 328 5 GCTCCA 3 CGAGGT 5 GCTCCAN sub 8 sub NNN span style padding left 2em span 3 3 CGAGGTN sub 8 sub N span style padding left 2em span NN 5 None as of 4 May 2018AocI 74 Anabaena sp 5 CCTNAGG 3 GGANTCC 5 CC span style padding left 2em span TNAGG 3 3 GGANT span style padding left 2em span CC 5 AyI Bse21I Bsu36I Eco81I Lmu60I OaNI MstII SauI SshAIAocII Anabaena sp 5 GDGCHC 3 CHCGDG 5 GDGCH span style padding left 2em span C 3 3 C span style padding left 2em span HCGDG 5 BmyI BsoCI Bsp1286I BspLS2I MhlI NspII SduIAorI 77 Acetobacter aceti orleanensis 5 CCWGG 3 GGWCC 5 CC span style padding left 2em span WGG 3 3 GGW span style padding left 2em span CC 5 BseBI BstNI BstOI Bst2UI EcoRII MvaI Sth117I TaiAor13HI 44 Acidiphilium organovorum 13H 5 TCCGGA 3 AGGCCT 5 T span style padding left 2em span CCGGA 3 3 AGGCC span style padding left 2em span T 5 AccIII BbvAIII BlfI BseAI BsiMI BspEI CauB3I Kpn2I MroI PtaIAor51HI 45 76 Acidiphilium organovorum 51H 5 AGCGCT 3 TCGCGA 5 AGC span style padding left 2em span GCT 3 3 TCG span style padding left 2em span CGA 5 AfeI AitI Eco47III FunIAosI Anabaena oscillarioides 5 TGCGCA 3 ACGCGT 5 TGC span style padding left 2em span GCA 3 3 ACG span style padding left 2em span CGT 5 Acc16I AviII FdiII FspI MstI NsbI PamI Pun14627IAosII Anabaena oscillarioides 5 GRCGYC 3 CYGCRG 5 GR span style padding left 2em span CGYC 3 3 CYGC span style padding left 2em span RG 5 AcyI AstWI BbiII BsaHI BstACI Hin1I HgiI HgiGI Msp17I PamIIApaI 11 30 46 Acetobacter pasteurianus pasteurianus 5 GGGCCC 3 CCCGGG 5 GGGCC span style padding left 2em span C 3 3 C span style padding left 2em span CCGGG 5 Bsp120I PpeI PspOMIApaBI 47 Acetobacter pasteurianus B 5 GCAN sub 5 sub TGC 3 CGTN sub 5 sub ACG 5 GCANNNNN span style padding left 2em span TGC 3 3 CGT span style padding left 2em span NNNNNACG 5 BstAPIApaCI Acetobacter pasteurianus C 5 GGATCC 3 CCTAGG 5 G span style padding left 2em span GATCC 3 3 CCTAG span style padding left 2em span G 5 AliI BamHI BnaI Bsp4009I BstI NspSAIV Pfl8I SolI SurIApaLI 48 49 Acetobacter pasteurianus 5 GTGCAC 3 CACGTG 5 G span style padding left 2em span TGCAC 3 3 CACGT span style padding left 2em span G 5 Alw44I SnoI VneIApaORI 86 Acetobacter pasteurianus 5 CCWGG 3 GGWCC 5 CC span style padding left 2em span WGG 3 3 GGW span style padding left 2em span CC 5 AjnI BstNI BstOI Bst1I Bst2I Fsp1604I MvaI Psp6I PspGIApeKI 50 Aeropyrum pernix K1 5 GCWGC 3 CGWCG 5 G span style padding left 2em span CWGC 3 3 CGWC span style padding left 2em span G 5 AceI SuiI Taq52I TseIApiI 2315 Arthrobacter picolinophilus 5 CTGCAG 3 GACGTC 5 CTGCA span style padding left 2em span G 3 3 G span style padding left 2em span ACGTC 5 Asp713I Bsp63I CfrA4I PaePI Pfl21I Psp23I PstI SflI Sst12IApoI 51 Arthrobacter protophormiae 5 RAATTY 3 YTTAAR 5 R span style padding left 2em span AATTY 3 3 YTTAA span style padding left 2em span R 5 AcsI CfaI FsiI XapI EcoRIAplI 25962 Arthrospira platensis NIES 39 5 CTGCAG 3 GACGTC 5 CTGCA span style padding left 2em span G 3 3 G span style padding left 2em span ACGTC 5 See this site for a complete list ApyI 93 Arthrobacter pyridinolis 5 CCWGG 3 GGWCC 5 CC span style padding left 2em span WGG 3 3 GGW span style padding left 2em span CC 5 BciBII BptI BseBI BstNI BstOI Bst2UI Bst38I Bst100I MvaIApyPI 14756 Arcanobacterium pyogenes 5 ATCGAC 3 TAGCTG 5 ATCGACN sub 17 sub NNN span style padding left 2em span 3 3 TAGCTGN sub 17 sub N span style padding left 2em span NN 5 None as of 4 August 2019AquI Agmenellum quadruplicatum PR 6 5 CYCGRG 3 GRGCYC 5 C span style padding left 2em span YCGRG 3 3 GRGCY span style padding left 2em span C 5 BcoI BmeT110I BsmAI BsoMAI BsoBI BstSI Eco27kI OfoIAquII 20238 Agmenellum quadruplicatum PR 6 5 GCCGNAC 3 CGGCNTG 5 GCCGNACN sub 17 sub NNN span style padding left 2em span 3 3 CGGCNTGN sub 17 sub N span style padding left 2em span NN 5 None as of 4 August 2019AquIII 20239 Agmenellum quadruplicatum PR 6 5 GAGGAG 3 CTCCTC 5 GAGGAGN sub 17 sub NNN span style padding left 2em span 3 3 CTCCTCN sub 17 sub N span style padding left 2em span NN 5 BseRIAquIV 20240 Agmenellum quadruplicatum PR 6 5 GRGGAAG 3 CYCCTTC 5 GRGGAAGN sub 16 sub NNN span style padding left 2em span 3 3 CYCCTTCN sub 16 sub N span style padding left 2em span NN 5 None as of 4 9 19ArsI 19405 Arthrobacter sp NTS 5 GACN sub 6 sub TTYG 3 CTGN sub 6 sub AARC 5 NNNNN span style padding left 2em span NN sub 7 sub GACN sub 6 sub TTYGN sub 5 sub NNNNNN span style padding left 2em span 3 3 span style padding left 2em span NNNNNNN sub 7 sub CTGN sub 6 sub AARCN sub 5 sub N span style padding left 2em span NNNNN 5 None as of Oct 28 2020AscI 11 52 Arthrobacter sp 5 GGCGCGCC 3 CCGCGCGG 5 GG span style padding left 2em span CGCGCC 3 3 CCGCGC span style padding left 2em span GG 5 PalAI SgsI AseI 53 96 Aquaspirillum serpens 5 ATTAAT 3 TAATTA 5 AT span style padding left 2em span TAAT 3 3 TAAT span style padding left 2em span TA 5 AsnI BpoAI PshBI Sru4DI VspIAseII 53 97 Aquaspirillum serpens 5 CCSGG 3 GGSCC 5 CC span style padding left 2em span SGG 3 3 GGS span style padding left 2em span CC 5 AhaI AsuC2I BcnI CauII EcoHI HgiS22I Kpn49kII Mgl14481I NciIAsiI Azotobacter sp 5 GGATCC 3 CCTAGG 5 G span style padding left 2em span GATCC 3 3 CCTAG span style padding left 2em span G 5 ApaCI BamHI BnaI Bsp4009I BstI OkrAI Pfl8I SurI Uba4009IAsiAI 2853 Arthrobacter sp A7359 5 ACCGGT 3 TGGCCA 5 A span style padding left 2em span CCGGT 3 3 TGGCC span style padding left 2em span A 5 AgeI AsiGI BshTI CsiAI CspAI PinAIAsiGI 10828 Arthrobacter sp G 5 ACCGGT 3 TGGCCA 5 A span style padding left 2em span CCGGT 3 3 TGGCC span style padding left 2em span A 5 AgeI AsiAI BshTI CsiAI CspAI PinAIAsiSI Arthrobacter sp 5 GCGATCGC 3 CGCTAGCG 5 GCGAT span style padding left 2em span CGC 3 3 CGC span style padding left 2em span TAGCG 5 RgaI SfaAI SgfIAsi256I 18472 Arthrobacter sp Ck256 5 GATC 3 CTAG 5 G span style padding left 2em span ATC 3 3 CTA span style padding left 2em span G 5 See this site for a complete list Asi372I 15803 Arthrobacter sp Mn372 5 ATGCAT 3 TACGTA 5 ATGCA span style padding left 2em span T 3 3 T span style padding left 2em span ACGTA 5 Ppu10I EcoT22I Mph1103I NsiI Zsp2I BfrBI Csp68KIII PinBI SepI SspD5IIAsnI 54 98 Arthrobacter sp N CM 5 ATTAAT 3 TAATTA 5 AT span style padding left 2em span TAAT 3 3 TAAT span style padding left 2em span TA 5 AseI BpoAI PshBI Sru4DI VspIAspI 55 Achromobacter sp 699 5 GACNNNGTC 3 CTGNNNCAG 5 GACN span style padding left 2em span NNGTC 3 3 CTGNN span style padding left 2em span NCAG 5 AtsI PflFI PsyI TelI Tth111IAsp10HI Acidiphilium sp 10H 5 TTCGAA 3 AAGCTT 5 TT span style padding left 2em span CGAA 3 3 AAGC span style padding left 2em span TT 5 AsuII BspT104I BstBI CbiI Csp68KII PlaII PpaAI Ssp1IAsp10HII 100 Acidiphilium sp 10H 5 CCAN sub 5 sub TGG 3 GGTN sub 5 sub ACC 5 CCANNNN span style padding left 2em span NTGG 3 3 GGTN span style padding left 2em span NNNNACC 5 AccB7I AcpII BasI Esp1396I PflBI PflMI Van91IAsp26HI 111 Acidiphilium sp 26H 5 GAATGC 3 CTTACG 5 GAATGCN span style padding left 2em span 3 3 CTTAC span style padding left 2em span GN 5 Asp27HI Asp35HI Asp36HI BsaMI BscCI BsmI PctIAsp27HI 112 Acidiphilium sp 27H 5 GAATGC 3 CTTACG 5 GAATGCN span style padding left 2em span 3 3 CTTAC span style padding left 2em span GN 5 Asp35HI Asp36HI Asp40HI BmaHI BsaMI BscCI BsmIAsp35HI 117 Acidiphilium sp 35H 5 GAATGC 3 CTTACG 5 GAATGCN span style padding left 2em span 3 3 CTTAC span style padding left 2em span GN 5 Asp36HI Asp40HI Asp50HI BsaMI BscCI BsmI Mva1269IAsp36HI 118 Acidiphilium sp 36H 5 GAATGC 3 CTTACG 5 GAATGCN span style padding left 2em span 3 3 CTTAC span style padding left 2em span GN 5 Asp40HI Asp50HI BmaHI BscCI BsmI Mva1269I PctIAsp40HI 120 Acidiphilium sp 40H 5 GAATGC 3 CTTACG 5 GAATGCN span style padding left 2em span 3 3 CTTAC span style padding left 2em span GN 5 Asp27HI Asp36HI Asp50HI BsaMI BscCI BsmI Mva1269IAsp50HI 122 Acidiphilium sp 50H 5 GAATGC 3 CTTACG 5 GAATGCN span style padding left 2em span 3 3 CTTAC span style padding left 2em span GN 5 Asp26HI Asp35HI Asp36HI BsaMI BscCI BsmI Mva1269IAsp700I 56 Achromobacter sp 700 5 GAAN sub 4 sub TTC 3 CTTN sub 4 sub AAG 5 GAANN span style padding left 2em span NNTTC 3 3 CTTNN span style padding left 2em span NNAAG 5 BbvAI MroXI PdmI XmnIAsp713I 131 Achromobacter sp 713 5 CTGCAG 3 GACGTC 5 CTGCA span style padding left 2em span G 3 3 G span style padding left 2em span ACGTC 5 AjoI AliAJI BloHII CfrA4I Ecl37kI MhaAI PstI SflI Srl5DI YenIAsp718I 57 Achromobacter sp 718 5 GGTACC 3 CCATGG 5 G span style padding left 2em span GTACC 3 3 CCATG span style padding left 2em span G 5 Acc65I AhaB8I KpnI SthIAsp745I Achromobacter sp 745 5 GGWCC 3 CCWGG 5 G span style padding left 2em span GWCC 3 3 CCWG span style padding left 2em span G 5 Bme18I BsrAI CauI Eco47I ErpI FdiI SinI VpaK11AI VpaK11BIAspAI Alcaligenes sp 5 GGTNACC 3 CCANTGG 5 G span style padding left 2em span GTNACC 3 3 CCANTG span style padding left 2em span G 5 AcrII Bse64I BseT10I BstEII BstPI Eco91I EcoO65I PspEIAspA2I 6959 Arthrobacter sp A2 5 CCTAGG 3 GGATCC 5 C span style padding left 2em span CTAGG 3 3 GGATC span style padding left 2em span C 5 AvrBII AvrII BlnI BspA2I XmaJIAspEI 58 Aureobacterium sp 5 GACN sub 5 sub GTC 3 CTGN sub 5 sub CAG 5 GACNNN span style padding left 2em span NNGTC 3 3 CTGNN span style padding left 2em span NNNCAG 5 AhdI BmeRI BspOVI DriI Eam1105I EclHKI NruGIAspHI 59 Achromobacter sp H 5 GWGCWC 3 CWCGWG 5 GWGCW span style padding left 2em span C 3 3 C span style padding left 2em span WCGWG 5 Alw21I Bbv12I Bsh45I BsiHKAI Bsm6I HgiAI MspV281IAspLEI Arthrobacter sp LE3860 5 GCGC 3 CGCG 5 GCG span style padding left 2em span C 3 3 C span style padding left 2em span GCG 5 BspLAI BstHHI CfoI FnuDIII HhaI Hin6I HinP1I HsoI HspAI SciNIAspMI 2977 Acinetobacter sp M 5 AGGCCT 3 TCCGGA 5 AGG span style padding left 2em span CCT 3 3 TCC span style padding left 2em span GGA 5 AatI Eco147I GdiI PceI SarI Sru30DI SseBI SteI StuIAspMDI Alcaligenes sp MD1 5 GATC 3 CTAG 5 span style padding left 2em span GATC 3 3 CTAG span style padding left 2em span 5 BfuCI Bsp143I BstMBI DpnII Kzo9I MboI NdeII Sau3AIAspNI Anabaena sp J3 5 GGNNCC 3 CCNNGG 5 GGN span style padding left 2em span NCC 3 3 CCN span style padding left 2em span NGG 5 BmiI BscBI BspLI NlaIV PspN4IAspS9I Arthrobacter sp S9 5 GGNCC 3 CCNGG 5 G span style padding left 2em span GNCC 3 3 CCNG span style padding left 2em span G 5 AsuI AvcI Bac36I Bal228I FmuI NspIV PspPI Sau96IAssI 4930 Arthrobacter sp 5 AGTACT 3 TCATGA 5 AGT span style padding left 2em span ACT 3 3 TCA span style padding left 2em span TGA 5 Acc113I BmcAI Bpa34I DpaI Eco255I RflFII ScaI ZrmIAstWI Anabaena sp 5 GRCGYC 3 CYGCRG 5 GR span style padding left 2em span CGYC 3 3 CYGC span style padding left 2em span RG 5 AcyI AsuIII BsaHI BstACI Hin1I HgiI HgiDI HgiGI Msp17IAsuI 60 Anabaena subcylindrica 5 GGNCC 3 CCNGG 5 G span style padding left 2em span GNCC 3 3 CCNG span style padding left 2em span G 5 AspS9I AvcI CcuI Cfr13I FmuI MaeK81II Nsp7121I PspPI UnbIAsuII 61 Anabaena subcylindrica 5 TTCGAA 3 AAGCTT 5 TT span style padding left 2em span CGAA 3 3 AAGC span style padding left 2em span TT 5 AcpI Bpu14I Bsp119I BspT104I Csp45I FspII LspI NspV SfuIAsuIII Anabaena subcylindrica 5 GRCGYC 3 CYGCRG 5 GR span style padding left 2em span CGYC 3 3 CYGC span style padding left 2em span RG 5 AcyI AsuIII BbiII BsaHI BstACI Hin1I HgiHII Hsp92I Msp17IAsuC2I 2901 Actinobacillus suis C2 5 CCSGG 3 GGSCC 5 CC span style padding left 2em span SGG 3 3 GGS span style padding left 2em span CC 5 AhaI AseII BcnI Eco1831I EcoHI Kpn49kII Mgl14481I NciIAsuHPI 62 2811 Actinobacillus suis HP 5 GGTGA 3 CCACT 5 GGTGAN sub 6 sub NN span style padding left 2em span 3 3 CCACTN sub 6 sub N span style padding left 2em span N 5 HphI SspD5IAsuNHI 62 Actinobacillus suis NH 5 GCTAGC 3 CGATCG 5 G span style padding left 2em span CTAGC 3 3 CGATC span style padding left 2em span G 5 AceII BmtI BspOI LlaG2I NheI PstNHIAtsI Aureobacterium testaceum 4842 5 GACNNNGTC 3 CTGNNNCAG 5 GACN span style padding left 2em span NNGTC 3 3 CTGNN span style padding left 2em span NCAG 5 AspI PflFI PsyI TelI Tth111IAvaI 14 63 64 Anabaena variabilis 5 CYCGRG 3 GRGCYC 5 C span style padding left 2em span YCGRG 3 3 GRGCY span style padding left 2em span C 5 AquI Ama87I BsiHKCI BsoBI BspLU4I Eco88I NspIII PlaAIAvaII 12 14 41 63 64 Anabaena variabilis 5 GGWCC 3 CCWGG 5 G span style padding left 2em span GWCC 3 3 CCWG span style padding left 2em span G 5 Bme18I Bme216I BsrAI Eco47I Kzo49I Psp03I SmuEI VpaK11AIAvcI Actinomyces violaceoniger cristalomycini 5 GGNCC 3 CCNGG 5 G span style padding left 2em span GNCC 3 3 CCNG span style padding left 2em span G 5 AspS9I BshKI BsiZI Bsp1894I BspBII BspF4I Bsu54I Pde12IAviII Anabaena variabillis halle 5 TGCGCA 3 ACGCGT 5 TGC span style padding left 2em span GCA 3 3 ACG span style padding left 2em span CGT 5 Acc16I AosI FdiII FspI MstI NsbI PamI Pun14627IAvrII 11 29 173 Anabaena variabilis UW 5 CCTAGG 3 GGATCC 5 C span style padding left 2em span CTAGG 3 3 GGATC span style padding left 2em span C 5 AspA2I AvrBII BlnI BspA2I XmaJIAvrBII 171 Arthrobacter variabilis 5 CCTAGG 3 GGATCC 5 C span style padding left 2em span CTAGG 3 3 GGATC span style padding left 2em span C 5 AspA2I AvrII BlnI BspA2I XmaJIAxyI 65 174 Acetobacter xylinus 5 CCTNAGG 3 GGANTCC 5 CC span style padding left 2em span TNAGG 3 3 GGANT span style padding left 2em span CC 5 AocI BliHKI Bse21I BspR7I Bsu36I CvnI Eco81I Lmu60I An HF version of this enzyme is available This list is incomplete you can help by adding missing items September 2010 Current list Article Top Whole list navigationNotes edit Smith HO Nathans D December 1973 Letter A suggested nomenclature for bacterial host modification and restriction systems and their enzymes J Mol Biol 81 3 419 23 doi 10 1016 0022 2836 73 90152 6 PMID 4588280 Roberts RJ Belfort M Bestor T Bhagwat AS Bickle TA Bitinaite J Blumenthal RM Degtyarev SK Dryden DT Dybvig K Firman K Gromova ES Gumport RI Halford SE Hattman S Heitman J Hornby DP Janulaitis A Jeltsch A Josephsen J Kiss A Klaenhammer TR Kobayashi I Kong H Kruger DH Lacks S Marinus MG Miyahara M Morgan RD Murray NE Nagaraja V Piekarowicz A Pingoud A Raleigh E Rao DN Reich N Repin VE Selker EU Shaw PC Stein DC Stoddard BL Szybalski W Trautner TA Van Etten JL Vitor JM Wilson GG Xu SY April 2003 A nomenclature for restriction enzymes DNA methyltransferases homing endonucleases and their genes Nucleic Acids Res 31 7 1805 12 doi 10 1093 nar gkg274 PMC 152790 PMID 12654995 Jeremy MB John LT Lubert S 2002 3 Protein Structure and Function Biochemistry San Francisco W H Freeman ISBN 0 7167 4684 0 Anfinsen C B 1973 Principles that Govern the Folding of Protein Chains Science 181 4096 223 30 Bibcode 1973Sci 181 223A doi 10 1126 science 181 4096 223 PMID 4124164 Kessler C Manta V August 1990 Specificity of restriction endonucleases and DNA modification methyltransferases a review Edition 3 Gene 92 1 2 1 248 doi 10 1016 0378 1119 90 90486 B PMID 2172084 Pingoud A Jeltsch A September 2001 Structure and function of type II restriction endonucleases Nucleic Acids Res 29 18 3705 27 doi 10 1093 nar 29 18 3705 PMC 55916 PMID 11557805 Grigaite R Maneliene Z Janulaitis A November 2002 AarI a restriction endonuclease from Arthrobacter aurescens SS2 322 which recognizes the novel non palindromic sequence 5 CACCTGC N 4 8 3 Nucleic Acids Res 30 21 123e 123 doi 10 1093 nar gnf122 PMC 135850 PMID 12409482 a b Sugisaki H Maekawa Y Kanazawa S Takanami M October 1982 New restriction endonucleases from Acetobacter aceti and Bacillus aneurinolyticus Nucleic Acids Res 10 19 5747 52 doi 10 1093 nar 10 19 5747 PMC 320926 PMID 6292849 Nwankwo DO Maunus RE Xu S January 1997 Cloning and expression of AatII restriction modification system in Escherichia coli Gene 185 1 105 9 doi 10 1016 S0378 1119 96 00641 5 PMID 9034320 a b Roberts RJ January 1983 Restriction and modification enzymes and their recognition sequences Nucleic Acids Res 11 1 r135 69 PMC 325705 PMID 6306557 a b c d e f Wei H Therrien C Blanchard A Guan S Zhu Z May 2008 The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases Nucleic Acids Res 36 9 e50 doi 10 1093 nar gkn182 PMC 2396408 PMID 18413342 S2CID 10463398 a b c d Nishigaki K Kaneko Y Wakuda H Husimi Y Tanaka T August 1985 Type II restriction endonucleases cleave single stranded DNAs in general Nucleic Acids Res 13 16 5747 60 doi 10 1093 nar 13 16 5747 PMC 321909 PMID 2994012 Shenoy S Daigle K Ehrlich KC Gehrke CW Ehrlich M June 1986 Hydrolysis by restriction endonucleases at their DNA recognition sequences substituted with mismatched base pairs Nucleic Acids Res 14 11 4407 20 PMC 311455 PMID 3012472 a b c d e Nelson M Christ C Schildkraut I July 1984 Alteration of apparent restriction endonuclease recognition specificities by DNA methylases Nucleic Acids Res 12 13 5165 73 doi 10 1093 nar 12 13 5165 PMC 318911 PMID 6087274 Nelson PS Papas TS Schweinfest CW February 1993 Restriction endonuclease cleavage of 5 methyl deoxycytosine hemimethylated DNA at high enzyme to substrate ratios Nucleic Acids Res 21 3 681 6 doi 10 1093 nar 21 3 681 PMC 309169 PMID 8441677 Kita K Hiraoka N Kimizuka F Obayashi A 1984 Determination of the cleavage site of restriction enzyme AccII using synthetic 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bj1850065 PMC 1161270 PMID 6246881 Yoshioka H Nakamura H Sasaki J Tahara Y Yamada Y 1983 Purification properties and recognition sequence of site specific restriction endonuclease from Acetobacter xylinus Agric Biol Chem 47 12 2871 9 doi 10 1271 bbb1961 47 2871 Retrieved from https en wikipedia org w index php title List of restriction enzyme cutting sites A amp oldid 1188093627, wikipedia, wiki, book, books, library,

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