The immobilized whole cell system is an alternative to enzyme immobilization. Unlike enzyme immobilization, where the enzyme is attached to a solid support (such as calcium alginate or activated PVA or activated PEI), in immobilized whole cell systems, the target cell is immobilized. Such methods may be implemented when the enzymes required are difficult or expensive to extract, an example being intracellular enzymes.[1][2] Also, if a series of enzymes are required in the reaction; whole cell immobilization may be used for convenience. This is only done on a commercial basis when the need for the product is more justified.
Multiple enzymes may be introduced into the reaction, thus eliminating the need for immobilization of multiple enzymes. Furthermore, intracellular enzymes need not be extracted prior to the reaction; they may be used directly. However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell.
Referencesedit
^Zaushitsyna, O.; Berillo, D.; Kirsebom, H.; Mattiasson, B. (2013). "Cryostructured and Crosslinked Viable Cells Forming Monoliths Suitable for Bioreactor Applications". Topics in Catalysis. 57 (5): 339. doi:10.1007/s11244-013-0189-9. S2CID 94773366.
^Aragão Börner, R.; Zaushitsyna, O.; Berillo, D.; Scaccia, N.; Mattiasson, B.; Kirsebom, H. (2014). "Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production". Process Biochemistry. 49: 10–18. doi:10.1016/j.procbio.2013.09.027.
immobilized, whole, cell, immobilized, whole, cell, system, alternative, enzyme, immobilization, unlike, enzyme, immobilization, where, enzyme, attached, solid, support, such, calcium, alginate, activated, activated, immobilized, whole, cell, systems, target, . The immobilized whole cell system is an alternative to enzyme immobilization Unlike enzyme immobilization where the enzyme is attached to a solid support such as calcium alginate or activated PVA or activated PEI in immobilized whole cell systems the target cell is immobilized Such methods may be implemented when the enzymes required are difficult or expensive to extract an example being intracellular enzymes 1 2 Also if a series of enzymes are required in the reaction whole cell immobilization may be used for convenience This is only done on a commercial basis when the need for the product is more justified Multiple enzymes may be introduced into the reaction thus eliminating the need for immobilization of multiple enzymes Furthermore intracellular enzymes need not be extracted prior to the reaction they may be used directly However some enzymes may be used for the metabolic needs of the cell leading to reduced yield of the cell References edit Zaushitsyna O Berillo D Kirsebom H Mattiasson B 2013 Cryostructured and Crosslinked Viable Cells Forming Monoliths Suitable for Bioreactor Applications Topics in Catalysis 57 5 339 doi 10 1007 s11244 013 0189 9 S2CID 94773366 Aragao Borner R Zaushitsyna O Berillo D Scaccia N Mattiasson B Kirsebom H 2014 Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production Process Biochemistry 49 10 18 doi 10 1016 j procbio 2013 09 027 See also editImmobilized enzyme nbsp This enzyme related article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Immobilized whole cell amp oldid 997203881, wikipedia, wiki, book, books, library,
