Somatic pairing of homologous chromosomes is similar to pre- and early meiotic pairing (see article: Homologous chromosome#In meiosis), and has been observed in Diptera[1] (Drosophila), and budding yeast,[2] for example (whether it evolved multiple times in metazoans is unclear[3]). Mammals show little pairing apart from in germline cells, taking place at specific loci, and under the control of developmental signalling (understood as a subset of other long-range interchromosomal interactions such as looping, and organisation into chromosomal territories).[4]
Dynamic chromosome pairing. The term “pairing” describes the spatial juxtaposition of entire homologous chromosomes, allelic sequences, and/or homologous sequences at non-allelic locations. (A, B) Homologous chromosomes can exist as paired throughout their entire length and the relative activities of pairing and anti-pairing factors determine the degree of global chromosome pairing. (C) Local pairing and anti-pairing factors can affect pairing status of specific genes or chromosomal regions. Local “pairing centers” can nucleate global pairing, but it is unclear whether factors regulating local pairing are different from global pairing factors. (D–F) In some cases, centromeres or telomeres from homologs can pair or cluster with non-homologous chromosomes, while there is no pairing along chromosome arms
While meiotic pairing has been extensively studied, the role of somatic pairing has remained less well understood, and even whether it is mechanistically related to meiotic pairing is unknown.[5]
Early workEdit
The first review of somatic pairing was made by Metz in 1916,[1] citing the first descriptions of pairing made in 1907[6] and 1908 by N. M. Stevens in germline cells, who noted:[7]
“it may therefore be true that pairing of homologous chromosomes occurs in connection with each mitosis throughout the life history of these insects” (p.215)
Stevens noted the potential for communication and a role in heredity.[7]
While meiotic homologous pairing subsequently became well studied, somatic pairing remained neglected due to what has been described as "limitations in cytological tools for measuring pairing and genetic tools for perturbing pairing dynamics".[5]
Recent findings from high-throughput screeningEdit
In 1998 it was determined that homologous pairing in Drosophila occurs through independent initiations (as opposed to a directed, 'processive zippering' motion).[4][8]
The first RNAi screen (based on DNA FISH[9]) was carried out to identify genes regulating D. melanogaster somatic pairing in 2012,[10] described at the time as providing "an extensive “parts list” of mostly novel factors". These comprised 40 pairing promoting genes and 65 'anti-pairing' genes (of which 2 and 1 were already known, respectively), many of which have human orthologs.[5]
An earlier RNAi screen in 2007 showed the disruption of Topoisomerase II activity impairs somatic pairing within Drosophila tissue culture,[11] indicating a role for topoisomerase-mediated organisation (or the direct interactions of topoisomerase enzymes) in pairing.[4] Condensin (despite dependent interactions with Topoisomerase II) is antagonistic to Drosophila homologous pairing.[12]
ReferencesEdit
^ abMetz, Charles W. (1916). "Chromosome studies on the Diptera. II. The paired association of chromosomes in the Diptera, and its significance". Journal of Experimental Zoology. 21 (2): 213–279. doi:10.1002/jez.1400210204. ISSN 0022-104X.
^Burgess, Sean M; Kleckner, Nancy; Weiner, Beth M (1999). "Somatic pairing of homologs in budding yeast: existence and modulation". Genes & Development. 13 (12): 1627–1641. doi:10.1101/gad.13.12.1627. ISSN 1549-5477. PMC316803. PMID 10385630.
^Joyce, Eric F; Erceg, Jelena; Wu, C-ting (2016). "Pairing and anti-pairing: a balancing act in the diploid genome". Current Opinion in Genetics & Development. 37: 119–128. doi:10.1016/j.gde.2016.03.002. ISSN 0959-437X. PMC4939289. PMID 27065367.
^ abcApte, Manasi S.; Meller, Victoria H. (2012). "Homologue Pairing in Flies and Mammals: Gene Regulation When Two Are Involved". Genetics Research International. 2012: 1–9. doi:10.1155/2012/430587. ISSN 2090-3154. PMC3335585. PMID 22567388.
^ abcCopenhaver, Gregory P.; Bosco, Giovanni (2012). "Chromosome Pairing: A Hidden Treasure No More". PLOS Genetics. 8 (5): e1002737. doi:10.1371/journal.pgen.1002737. ISSN 1553-7404. PMC3359980. PMID 22654678.
^Stevens, N. M. (1907) The chromosomes of Drosophila ampelophila. Proc. VII Internat. Zool. Cong
^ abStevens, N. M. (1908). "A study of the germ cells of certain diptera, with reference to the heterochromosomes and the phenomena of synapsis". Journal of Experimental Zoology. 5 (3): 359–374. doi:10.1002/jez.1400050304. ISSN 0022-104X.
^Fung, Jennifer C.; Marshall, Wallace F.; Dernburg, Abby; Agard, David A.; Sedat, John W. (1998). "Homologous Chromosome Pairing inDrosophila melanogasterProceeds through Multiple Independent Initiations". The Journal of Cell Biology. 141 (1): 5–20. CiteSeerX10.1.1.431.4960. doi:10.1083/jcb.141.1.5. ISSN 0021-9525. PMC2132734. PMID 9531544.
^Gonzalez, Inma; Mateos-Langerak, Julio; Thomas, Aubin; Cheutin, Thierry; Cavalli, Giacomo (2014). "Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen". Molecular Cell. 54 (3): 485–499. doi:10.1016/j.molcel.2014.03.004. ISSN 1097-2765. PMID 24703951.
^Joyce, Eric F.; Williams, Benjamin R.; Xie, Tiao; Wu, C.-ting (2012). "Identification of Genes That Promote or Antagonize Somatic Homolog Pairing Using a High-Throughput FISH–Based Screen". PLOS Genetics. 8 (5): e1002667. doi:10.1371/journal.pgen.1002667. ISSN 1553-7404. PMC3349724. PMID 22589731.
^Williams, B. R.; Bateman, J. R.; Novikov, N. D.; Wu, C.-T. (2007). "Disruption of Topoisomerase II Perturbs Pairing in Drosophila Cell Culture". Genetics. 177 (1): 31–46. doi:10.1534/genetics.107.076356. ISSN 0016-6731. PMC2013714. PMID 17890361.
^Hartl, T. A.; Smith, H. F.; Bosco, G. (2008). "Chromosome Alignment and Transvection Are Antagonized by Condensin II". Science. 322 (5906): 1384–1387. Bibcode:2008Sci...322.1384H. doi:10.1126/science.1164216. ISSN 0036-8075. PMID 19039137. S2CID 5154197.
October 19, 2023
homologous, somatic, pairing, somatic, pairing, homologous, chromosomes, similar, early, meiotic, pairing, article, homologous, chromosome, meiosis, been, observed, diptera, drosophila, budding, yeast, example, whether, evolved, multiple, times, metazoans, unc. Somatic pairing of homologous chromosomes is similar to pre and early meiotic pairing see article Homologous chromosome In meiosis and has been observed in Diptera 1 Drosophila and budding yeast 2 for example whether it evolved multiple times in metazoans is unclear 3 Mammals show little pairing apart from in germline cells taking place at specific loci and under the control of developmental signalling understood as a subset of other long range interchromosomal interactions such as looping and organisation into chromosomal territories 4 Dynamic chromosome pairing The term pairing describes the spatial juxtaposition of entire homologous chromosomes allelic sequences and or homologous sequences at non allelic locations A B Homologous chromosomes can exist as paired throughout their entire length and the relative activities of pairing and anti pairing factors determine the degree of global chromosome pairing C Local pairing and anti pairing factors can affect pairing status of specific genes or chromosomal regions Local pairing centers can nucleate global pairing but it is unclear whether factors regulating local pairing are different from global pairing factors D F In some cases centromeres or telomeres from homologs can pair or cluster with non homologous chromosomes while there is no pairing along chromosome armsWhile meiotic pairing has been extensively studied the role of somatic pairing has remained less well understood and even whether it is mechanistically related to meiotic pairing is unknown 5 Early work EditThe first review of somatic pairing was made by Metz in 1916 1 citing the first descriptions of pairing made in 1907 6 and 1908 by N M Stevens in germline cells who noted 7 it may therefore be true that pairing of homologous chromosomes occurs in connection with each mitosis throughout the life history of these insects p 215 Stevens noted the potential for communication and a role in heredity 7 While meiotic homologous pairing subsequently became well studied somatic pairing remained neglected due to what has been described as limitations in cytological tools for measuring pairing and genetic tools for perturbing pairing dynamics 5 Recent findings from high throughput screening EditIn 1998 it was determined that homologous pairing in Drosophila occurs through independent initiations as opposed to a directed processive zippering motion 4 8 The first RNAi screen based on DNA FISH 9 was carried out to identify genes regulating D melanogaster somatic pairing in 2012 10 described at the time as providing an extensive parts list of mostly novel factors These comprised 40 pairing promoting genes and 65 anti pairing genes of which 2 and 1 were already known respectively many of which have human orthologs 5 An earlier RNAi screen in 2007 showed the disruption of Topoisomerase II activity impairs somatic pairing within Drosophila tissue culture 11 indicating a role for topoisomerase mediated organisation or the direct interactions of topoisomerase enzymes in pairing 4 Condensin despite dependent interactions with Topoisomerase II is antagonistic to Drosophila homologous pairing 12 References Edit a b Metz Charles W 1916 Chromosome studies on the Diptera II The paired association of chromosomes in the Diptera and its significance Journal of Experimental Zoology 21 2 213 279 doi 10 1002 jez 1400210204 ISSN 0022 104X Burgess Sean M Kleckner Nancy Weiner Beth M 1999 Somatic pairing of homologs in budding yeast existence and modulation Genes amp Development 13 12 1627 1641 doi 10 1101 gad 13 12 1627 ISSN 1549 5477 PMC 316803 PMID 10385630 Joyce Eric F Erceg Jelena Wu C ting 2016 Pairing and anti pairing a balancing act in the diploid genome Current Opinion in Genetics amp Development 37 119 128 doi 10 1016 j gde 2016 03 002 ISSN 0959 437X PMC 4939289 PMID 27065367 a b c Apte Manasi S Meller Victoria H 2012 Homologue Pairing in Flies and Mammals Gene Regulation When Two Are Involved Genetics Research International 2012 1 9 doi 10 1155 2012 430587 ISSN 2090 3154 PMC 3335585 PMID 22567388 a b c Copenhaver Gregory P Bosco Giovanni 2012 Chromosome Pairing A Hidden Treasure No More PLOS Genetics 8 5 e1002737 doi 10 1371 journal pgen 1002737 ISSN 1553 7404 PMC 3359980 PMID 22654678 Stevens N M 1907 The chromosomes of Drosophila ampelophila Proc VII Internat Zool Cong a b Stevens N M 1908 A study of the germ cells of certain diptera with reference to the heterochromosomes and the phenomena of synapsis Journal of Experimental Zoology 5 3 359 374 doi 10 1002 jez 1400050304 ISSN 0022 104X Fung Jennifer C Marshall Wallace F Dernburg Abby Agard David A Sedat John W 1998 Homologous Chromosome Pairing inDrosophila melanogasterProceeds through Multiple Independent Initiations The Journal of Cell Biology 141 1 5 20 CiteSeerX 10 1 1 431 4960 doi 10 1083 jcb 141 1 5 ISSN 0021 9525 PMC 2132734 PMID 9531544 Gonzalez Inma Mateos Langerak Julio Thomas Aubin Cheutin Thierry Cavalli Giacomo 2014 Identification of Regulators of the Three Dimensional Polycomb Organization by a Microscopy Based Genome wide RNAi Screen Molecular Cell 54 3 485 499 doi 10 1016 j molcel 2014 03 004 ISSN 1097 2765 PMID 24703951 Joyce Eric F Williams Benjamin R Xie Tiao Wu C ting 2012 Identification of Genes That Promote or Antagonize Somatic Homolog Pairing Using a High Throughput FISH Based Screen PLOS Genetics 8 5 e1002667 doi 10 1371 journal pgen 1002667 ISSN 1553 7404 PMC 3349724 PMID 22589731 Williams B R Bateman J R Novikov N D Wu C T 2007 Disruption of Topoisomerase II Perturbs Pairing in Drosophila Cell Culture Genetics 177 1 31 46 doi 10 1534 genetics 107 076356 ISSN 0016 6731 PMC 2013714 PMID 17890361 Hartl T A Smith H F Bosco G 2008 Chromosome Alignment and Transvection Are Antagonized by Condensin II Science 322 5906 1384 1387 Bibcode 2008Sci 322 1384H doi 10 1126 science 1164216 ISSN 0036 8075 PMID 19039137 S2CID 5154197 Retrieved from https en wikipedia org w index php title Homologous somatic pairing amp oldid 1093036140, wikipedia, wiki, book, books, library,
