A small intestine sample stained using the Grocott's methenamine silver stain demonstrating histoplasma (black round yeast with narrow budding) in a granuloma
It is used widely as a screen for fungalorganisms. It is particularly useful in staining carbohydrates.
The cell walls of these organisms are outlined by the brown to black stain.
The principle of GMS is the reduction of silver ions, which renders the fungal cell wall black. The fungal cell wall commonly contains polysaccharides. In a GMS procedure, chromic acid is first used to oxidize polysaccharides, generating aldehydes. Then Grocott's alkaline hexamine-silver solution is applied, where the silver ions are reduced to black amorphous silver. The reduction reaction by the fungal cell wall is often known as argentaffin reaction.[2]
^Nassar A, Zapata M, Little JV, Siddiqui MT (November 2006). "Utility of reflex gomori methenamine silver staining for Pneumocystis jirovecii on bronchoalveolar lavage cytologic specimens: A review". Diagn. Cytopathol. 34 (11): 719–23. doi:10.1002/dc.20540. PMID 17041954.
grocott, methenamine, silver, stain, pathology, grocott, gömöri, methenamine, silver, stain, abbreviated, popular, staining, method, histology, stain, originally, named, after, györgy, gömöri, hungarian, physician, developed, stain, small, intestine, sample, s. In pathology the Grocott Gomori s methenamine silver stain abbreviated GMS is a popular staining method in histology The stain was originally named after Gyorgy Gomori the Hungarian physician who developed the stain A small intestine sample stained using the Grocott s methenamine silver stain demonstrating histoplasma black round yeast with narrow budding in a granulomaIt is used widely as a screen for fungal organisms It is particularly useful in staining carbohydrates It can be used to identify the yeast like fungus Pneumocystis jiroveci 1 which causes a form of pneumonia called Pneumocystis pneumonia PCP or pneumocystosis The cell walls of these organisms are outlined by the brown to black stain The principle of GMS is the reduction of silver ions which renders the fungal cell wall black The fungal cell wall commonly contains polysaccharides In a GMS procedure chromic acid is first used to oxidize polysaccharides generating aldehydes Then Grocott s alkaline hexamine silver solution is applied where the silver ions are reduced to black amorphous silver The reduction reaction by the fungal cell wall is often known as argentaffin reaction 2 See also EditMethenamineReferences Edit Nassar A Zapata M Little JV Siddiqui MT November 2006 Utility of reflex gomori methenamine silver staining for Pneumocystis jirovecii on bronchoalveolar lavage cytologic specimens A review Diagn Cytopathol 34 11 719 23 doi 10 1002 dc 20540 PMID 17041954 Grocott Gomori s Methenamine Silver Staining Microbe Notes 2021 02 08 Retrieved 2022 01 03 Retrieved from https en wikipedia org w index php title Grocott 27s methenamine silver stain amp oldid 1158211667, wikipedia, wiki, book, books, library,
