Guanylyl transferases are enzymes that transfer a guanosine mono phosphate group, usually from GTP to another molecule, releasing pyrophosphate. Many eukaryotic guanylyl transferases are capping enzymes that catalyze the formation of the 5' cap in the co-transcriptional modification of messenger RNA. Because the 5' end of the RNA molecule ends in a phosphate group, the bond formed between the RNA and the GTP molecule is an unusual 5'-5' triphosphate linkage, instead of the 3'-5' linkages between the other nucleotides that form an RNA strand. In capping enzymes, a highly conserved lysine residue serves as the catalytic residue that forms a covalent enzyme-GMP complex.[1]
The transfer RNA (tRNA) for histidine is unique among eukaryotic tRNAs in requiring the addition of a guanine nucleotide before being aminoacylated by the histidine tRNA synthetase. The yeast guanylyl transferase specific to tRNAHis is unique in being the only known non-tRNA synthetase enzyme that specifically recognizes the tRNA anticodon.[2]
Guanylyl transferases also exist for transferring guanosine nucleotides to sugar molecules, such as mannose and fucose.
^Fresco LD, Buratowski S. (1994). Active site of the mRNA-capping enzyme guanylyltransferase from Saccharomyces cerevisiae: similarity to the nucleotidyl attachment motif of DNA and RNA ligases. Proc Natl Acad Sci USA 91(14): 6624–6628.
^Jackman JE, Phizicky EM. (2006). tRNAHis guanylyltransferase adds G–1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases. RNA 12:1007-1014.
guanylyltransferase, guanylyl, transferases, enzymes, that, transfer, guanosine, mono, phosphate, group, usually, from, another, molecule, releasing, pyrophosphate, many, eukaryotic, guanylyl, transferases, capping, enzymes, that, catalyze, formation, transcri. Guanylyl transferases are enzymes that transfer a guanosine mono phosphate group usually from GTP to another molecule releasing pyrophosphate Many eukaryotic guanylyl transferases are capping enzymes that catalyze the formation of the 5 cap in the co transcriptional modification of messenger RNA Because the 5 end of the RNA molecule ends in a phosphate group the bond formed between the RNA and the GTP molecule is an unusual 5 5 triphosphate linkage instead of the 3 5 linkages between the other nucleotides that form an RNA strand In capping enzymes a highly conserved lysine residue serves as the catalytic residue that forms a covalent enzyme GMP complex 1 The transfer RNA tRNA for histidine is unique among eukaryotic tRNAs in requiring the addition of a guanine nucleotide before being aminoacylated by the histidine tRNA synthetase The yeast guanylyl transferase specific to tRNAHis is unique in being the only known non tRNA synthetase enzyme that specifically recognizes the tRNA anticodon 2 Guanylyl transferases also exist for transferring guanosine nucleotides to sugar molecules such as mannose and fucose See also EditmRNA guanylyltransferaseExternal links EditEC number 2 7 7 nucleotidyltransferasesReferences Edit Fresco LD Buratowski S 1994 Active site of the mRNA capping enzyme guanylyltransferase from Saccharomyces cerevisiae similarity to the nucleotidyl attachment motif of DNA and RNA ligases Proc Natl Acad Sci USA 91 14 6624 6628 Jackman JE Phizicky EM 2006 tRNAHis guanylyltransferase adds G 1 to the 5 end of tRNAHis by recognition of the anticodon one of several features unexpectedly shared with tRNA synthetases RNA 12 1007 1014 Portal Biology This EC 2 7 enzyme related article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Guanylyltransferase amp oldid 917406626, wikipedia, wiki, book, books, library,
