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Gene stacking

March 20, 2024

Gene stacking is the combination of more than one gene for plant disease resistance, or crop productivity, or other horticultural traits.[T 1] In plant breeding traditionally that means breeding those genes in, but increasingly also can mean genetic engineering.[T 1] This can be achieved a few different ways, and gene pyramiding is one of those methods.[T 2] Stacking of transgenes is yet more difficult than stacking natural genes, but especially in the case of pest resistance genes which require a significant financial investment to insert, is advantageous over other methods.[1] Pathosystems with rapid evolution in the pathogen have long been considered good targets of stacking, to broaden and prolong resistance.[2][3]

Assaying for successful insertion of R genes is much more difficult than for one at a time.[4] A simple challenge assay will only tell between complete failure/some unknown degree of success.[4] Building a new technique specifically for the multiple genes you are attempting to insert may be necessary.[4]

References edit

  1. ^ Halpin, Claire (2005-02-03). "Gene stacking in transgenic plants - the challenge for 21st century plant biotechnology". Plant Biotechnology Journal. Wiley Publishing. 3 (2): 141–155. doi:10.1111/j.1467-7652.2004.00113.x. ISSN 1467-7644. PMID 17173615.
  2. ^ Luo, Ming; Xie, Liqiong; Chakraborty, Soma; Wang, Aihua; Matny, Oadi; Jugovich, Michelle; Kolmer, James A.; Richardson, Terese; Bhatt, Dhara; Hoque, Mohammad; Patpour, Mehran; Sørensen, Chris; Ortiz, Diana; Dodds, Peter; Steuernagel, Burkhard; Wulff, Brande B. H.; Upadhyaya, Narayana M.; Mago, Rohit; Periyannan, Sambasivam; Lagudah, Evans; Freedman, Roger; Lynne Reuber, T.; Steffenson, Brian J.; Ayliffe, Michael (2020-12-09). "A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat". Nature Portfolio Bioengineering Community. Retrieved 2021-07-02.
  3. ^ Luo, Ming; Xie, Liqiong; Chakraborty, Soma; Wang, Aihua; Matny, Oadi; Jugovich, Michelle; Kolmer, James A.; Richardson, Terese; Bhatt, Dhara; Hoque, Mohammad; Patpour, Mehran; Sørensen, Chris; Ortiz, Diana; Dodds, Peter; Steuernagel, Burkhard; Wulff, Brande B. H.; Upadhyaya, Narayana M.; Mago, Rohit; Periyannan, Sambasivam; Lagudah, Evans; Freedman, Roger; Lynne Reuber, T.; Steffenson, Brian J.; Ayliffe, Michael (2021-01-14). "Innovative gene stacks enhance wheat rust resistance". John Innes Centre. Retrieved 2021-07-02.
  4. ^ a b c Zhu, Suxian; Li, Ying; Vossen, Jack H.; Visser, Richard G. F.; Jacobsen, Evert (2011-04-10). "Functional stacking of three resistance genes against Phytophthora infestans in potato". Transgenic Research. Springer Science and Business Media LLC. 21 (1): 89–99. doi:10.1007/s11248-011-9510-1. ISSN 0962-8819. PMC 3264857. PMID 21479829.
  • Taverniers, Isabel; Papazova, Nina; Bertheau, Yves; De Loose, Marc; Holst-Jensen, Arne (2008). "Gene stacking in transgenic plants: towards compliance between definitions, terminology, and detection within the EU regulatory framework". Environmental Biosafety Research. EDP Sciences. 7 (4): 197–218. doi:10.1051/ebr:2008018. ISSN 1635-7922. PMID 19081008.
  1. ^ a b p. 199, "Independent of modern biotechnology, “stacking” traditionally refers to the natural addition of different plant properties by genetic crossing. Modern biotechnology has broadened the options for stacking to include more taxonomically diverse sources, a wider selection of genes and regulatory elements, and consequently of traits."
  2. ^ p. 197, "The term gene pyramiding is used in agricultural research to describe a breeding approach to achieve pest control and higher crop yield. It is essentially a way of identifying and introducing multiple genes, which each impart resistance to an independent insect/microbial pest/weed etc., or impart resistance to a single pest through independent host pathways."


 

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March 20, 2024
gene, stacking, combination, more, than, gene, plant, disease, resistance, crop, productivity, other, horticultural, traits, plant, breeding, traditionally, that, means, breeding, those, genes, increasingly, also, mean, genetic, engineering, this, achieved, di. Gene stacking is the combination of more than one gene for plant disease resistance or crop productivity or other horticultural traits T 1 In plant breeding traditionally that means breeding those genes in but increasingly also can mean genetic engineering T 1 This can be achieved a few different ways and gene pyramiding is one of those methods T 2 Stacking of transgenes is yet more difficult than stacking natural genes but especially in the case of pest resistance genes which require a significant financial investment to insert is advantageous over other methods 1 Pathosystems with rapid evolution in the pathogen have long been considered good targets of stacking to broaden and prolong resistance 2 3 Assaying for successful insertion of R genes is much more difficult than for one at a time 4 A simple challenge assay will only tell between complete failure some unknown degree of success 4 Building a new technique specifically for the multiple genes you are attempting to insert may be necessary 4 References edit Halpin Claire 2005 02 03 Gene stacking in transgenic plants the challenge for 21st century plant biotechnology Plant Biotechnology Journal Wiley Publishing 3 2 141 155 doi 10 1111 j 1467 7652 2004 00113 x ISSN 1467 7644 PMID 17173615 Luo Ming Xie Liqiong Chakraborty Soma Wang Aihua Matny Oadi Jugovich Michelle Kolmer James A Richardson Terese Bhatt Dhara Hoque Mohammad Patpour Mehran Sorensen Chris Ortiz Diana Dodds Peter Steuernagel Burkhard Wulff Brande B H Upadhyaya Narayana M Mago Rohit Periyannan Sambasivam Lagudah Evans Freedman Roger Lynne Reuber T Steffenson Brian J Ayliffe Michael 2020 12 09 A five transgene cassette confers broad spectrum resistance to a fungal rust pathogen in wheat Nature Portfolio Bioengineering Community Retrieved 2021 07 02 Luo Ming Xie Liqiong Chakraborty Soma Wang Aihua Matny Oadi Jugovich Michelle Kolmer James A Richardson Terese Bhatt Dhara Hoque Mohammad Patpour Mehran Sorensen Chris Ortiz Diana Dodds Peter Steuernagel Burkhard Wulff Brande B H Upadhyaya Narayana M Mago Rohit Periyannan Sambasivam Lagudah Evans Freedman Roger Lynne Reuber T Steffenson Brian J Ayliffe Michael 2021 01 14 Innovative gene stacks enhance wheat rust resistance John Innes Centre Retrieved 2021 07 02 a b c Zhu Suxian Li Ying Vossen Jack H Visser Richard G F Jacobsen Evert 2011 04 10 Functional stacking of three resistance genes against Phytophthora infestans in potato Transgenic Research Springer Science and Business Media LLC 21 1 89 99 doi 10 1007 s11248 011 9510 1 ISSN 0962 8819 PMC 3264857 PMID 21479829 Taverniers Isabel Papazova Nina Bertheau Yves De Loose Marc Holst Jensen Arne 2008 Gene stacking in transgenic plants towards compliance between definitions terminology and detection within the EU regulatory framework Environmental Biosafety Research EDP Sciences 7 4 197 218 doi 10 1051 ebr 2008018 ISSN 1635 7922 PMID 19081008 a b p 199 Independent of modern biotechnology stacking traditionally refers to the natural addition of different plant properties by genetic crossing Modern biotechnology has broadened the options for stacking to include more taxonomically diverse sources a wider selection of genes and regulatory elements and consequently of traits p 197 The term gene pyramiding is used in agricultural research to describe a breeding approach to achieve pest control and higher crop yield It is essentially a way of identifying and introducing multiple genes which each impart resistance to an independent insect microbial pest weed etc or impart resistance to a single pest through independent host pathways nbsp This genetics article is a stub You can help Wikipedia by expanding it vte nbsp This horticulture article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Gene stacking amp oldid 1062329357, wikipedia, wiki, book, books, library,

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