Since only GDP-L-gulose (the C5-epimer of GDP-D-mannose) was found in the reaction mixture, it was postulated that the enzyme performs the C5-epimerization prior to the C3-epimerization. However, GDP-D-altrose was recently found as a reaction product, which means that both reaction routes can occur: C5-prior-to-C3 and C3-prior-to-C5. This also means that the GDP-mannose 3,5-epimerase has three reaction products, namely the main product GDP-L-galactose (C3,5-epimer) and two sideproducts GDP-L-gulose (C5-epimer) + GDP-D-altrose (C3-epimer).
This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is GDP-mannose 3,5-epimerase. Other names in common use include GDP-D-mannose:GDP-L-galactose epimerase, guanosine 5'-diphosphate D-mannose:guanosine 5'-diphosphate, and L-galactose epimerase. This enzyme participates in ascorbate and aldarate metabolism.
Structural studiesedit
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 2C54, 2C59, 2C5A, and 2C5E.
Referencesedit
Barber GA, Hebda PA (1982). "GDP-D-mannose: GDP-L-galactose epimerase from Chlorella pyrenoidosa". Methods Enzymol. Methods in Enzymology. 83: 522–5. doi:10.1016/0076-6879(82)83046-2. ISBN978-0-12-181983-5. PMID 7098948.
Hebda PA, Behrman EJ, Barber GA (1979). "The guanosine 5'-diphosphate D-mannose: guanosine 5'-diphosphate L-galactose epimerase of Chlorella pyrenoidosa. Chemical synthesis of guanosine 5'-diphosphate L-galactose and further studies of the enzyme and the reaction it catalyzes". Arch. Biochem. Biophys. 194 (2): 496–502. doi:10.1016/0003-9861(79)90644-1. PMID 443816.
Gevaert O, Van Overtveldt S, Beerens K, Desmet T (2019). "Characterization of the First Bacterial and Thermostable GDP-Mannose 3,5-Epimerase". Int. J. Mol. Sci. 20 (14): E3530. doi:10.3390/ijms20143530. PMC6678494. PMID 31330931.
Gevaert O, Van Overtveldt S, Da Costa M, Beerens K, Desmet T (2020). "GDP-altrose as novel product of GDP-mannose 3,5-epimerase: Revisiting its reaction mechanism". Int J Biol Macromol. 165 (Pt B): 1862–1868. doi:10.1016/j.ijbiomac.2020.10.067. PMID 33075338.
mannose, epimerase, enzymology, enzyme, that, catalyzes, chemical, reactionidentifiersec, 18cas, 72162, 4databasesintenzintenz, viewbrendabrenda, entryexpasynicezyme, viewkeggkegg, entrymetacycmetabolic, pathwaypriamprofilepdb, structuresrcsb, pdbe, pdbsumgene. In enzymology a GDP mannose 3 5 epimerase EC 5 1 3 18 is an enzyme that catalyzes the chemical reactionGDP mannose 3 5 epimeraseIdentifiersEC no 5 1 3 18CAS no 72162 82 4DatabasesIntEnzIntEnz viewBRENDABRENDA entryExPASyNiceZyme viewKEGGKEGG entryMetaCycmetabolic pathwayPRIAMprofilePDB structuresRCSB PDB PDBe PDBsumGene OntologyAmiGO QuickGOSearchPMCarticlesPubMedarticlesNCBIproteins GDP mannose displaystyle rightleftharpoons GDP L galactose GDP L gulose initial reaction overview updated in 2020 see below Hence this enzyme has one substrate GDP mannose and two products GDP L galactose and GDP L guloseSince only GDP L gulose the C5 epimer of GDP D mannose was found in the reaction mixture it was postulated that the enzyme performs the C5 epimerization prior to the C3 epimerization However GDP D altrose was recently found as a reaction product which means that both reaction routes can occur C5 prior to C3 and C3 prior to C5 This also means that the GDP mannose 3 5 epimerase has three reaction products namely the main product GDP L galactose C3 5 epimer and two sideproducts GDP L gulose C5 epimer GDP D altrose C3 epimer GDP D mannose displaystyle rightleftharpoons GDP L galactose C3 5 epimer GDP L gulose C5 epimer GDP D altrose C3 epimer reaction overview update in 2020This enzyme belongs to the family of isomerases specifically those racemases and epimerases acting on carbohydrates and derivatives The systematic name of this enzyme class is GDP mannose 3 5 epimerase Other names in common use include GDP D mannose GDP L galactose epimerase guanosine 5 diphosphate D mannose guanosine 5 diphosphate and L galactose epimerase This enzyme participates in ascorbate and aldarate metabolism Structural studies editAs of late 2007 4 structures have been solved for this class of enzymes with PDB accession codes 2C54 2C59 2C5A and 2C5E References editBarber GA Hebda PA 1982 GDP D mannose GDP L galactose epimerase from Chlorella pyrenoidosa Methods Enzymol Methods in Enzymology 83 522 5 doi 10 1016 0076 6879 82 83046 2 ISBN 978 0 12 181983 5 PMID 7098948 Hebda PA Behrman EJ Barber GA 1979 The guanosine 5 diphosphate D mannose guanosine 5 diphosphate L galactose epimerase of Chlorella pyrenoidosa Chemical synthesis of guanosine 5 diphosphate L galactose and further studies of the enzyme and the reaction it catalyzes Arch Biochem Biophys 194 2 496 502 doi 10 1016 0003 9861 79 90644 1 PMID 443816 Gevaert O Van Overtveldt S Beerens K Desmet T 2019 Characterization of the First Bacterial and Thermostable GDP Mannose 3 5 Epimerase Int J Mol Sci 20 14 E3530 doi 10 3390 ijms20143530 PMC 6678494 PMID 31330931 Gevaert O Van Overtveldt S Da Costa M Beerens K Desmet T 2020 GDP altrose as novel product of GDP mannose 3 5 epimerase Revisiting its reaction mechanism Int J Biol Macromol 165 Pt B 1862 1868 doi 10 1016 j ijbiomac 2020 10 067 PMID 33075338 Portal nbsp Biology This isomerase article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title GDP mannose 3 5 epimerase amp oldid 1172347460, wikipedia, wiki, book, books, library,
