Endoprotease B, isoform 2 (EP-B2) is a cysteine protease found in barley. In studies, it has shown promise as a possible therapeutic for coeliac disease.[1] Aspergillopepsin from Aspergillus niger has been shown to enhance the effects of EP-B2.[2]
Referencesedit
^Vora, H.; McIntire, J.; Kumar, P.; Deshpande, M.; Khosla, C. (2007). "A scaleable manufacturing process for pro-EP-B2, a cysteine protease from barley indicated for celiac sprue". Biotechnology and Bioengineering. 98 (1): 177–185. doi:10.1002/bit.21423. PMID 17385743. S2CID 19581223.
^Ehren, J.; Morón, B.; Martin, E.; Bethune, M.; Gray, G.; Khosla, C.; El-Shemy, H. A. (2009). "A food-grade enzyme preparation with modest gluten detoxification properties". PLOS ONE. 4 (7): e6313. Bibcode:2009PLoSO...4.6313E. doi:10.1371/journal.pone.0006313. PMC2708912. PMID 19621078.
Further readingedit
Bethune, M.; Ribka, E.; Khosla, C.; Sestak, K.; Bozza, P. (2008). "Transepithelial transport and enzymatic detoxification of gluten in gluten-sensitive rhesus macaques". PLOS ONE. 3 (3): e1857. Bibcode:2008PLoSO...3.1857B. doi:10.1371/journal.pone.0001857. PMC2267209. PMID 18365012.
Gass, J.; Bethune, M.; Siegel, M.; Spencer, A.; Khosla, C. (2007). "Combination enzyme therapy for gastric digestion of dietary gluten in patients with celiac sprue". Gastroenterology. 133 (2): 472–480. doi:10.1053/j.gastro.2007.05.028. PMID 17681168.
Siegel, M.; Bethune, M.; Gass, J.; Ehren, J.; Xia, J.; Johannsen, A.; Stuge, T.; Gray, G.; Lee, P.; Khosla, C. (2006). "Rational design of combination enzyme therapy for celiac sprue". Chemistry & Biology. 13 (6): 649–658. doi:10.1016/j.chembiol.2006.04.009. PMID 16793522.
Bethune, M.; Strop, P.; Tang, Y.; Sollid, L.; Khosla, C. (2006). "Heterologous expression, purification, refolding, and structural-functional characterization of EP-B2, a self-activating barley cysteine endoprotease". Chemistry & Biology. 13 (6): 637–647. doi:10.1016/j.chembiol.2006.04.008. PMID 16793521.
Gass, J.; Vora, H.; Bethune, M.; Gray, G.; Khosla, C. (2006). "Effect of barley endoprotease EP-B2 on gluten digestion in the intact rat". The Journal of Pharmacology and Experimental Therapeutics. 318 (3): 1178–1186. doi:10.1124/jpet.106.104315. PMID 16757540. S2CID 33662709.
endoprotease, isoform, cysteine, protease, found, barley, studies, shown, promise, possible, therapeutic, coeliac, disease, aspergillopepsin, from, aspergillus, niger, been, shown, enhance, effects, references, edit, vora, mcintire, kumar, deshpande, khosla, 2. Endoprotease B isoform 2 EP B2 is a cysteine protease found in barley In studies it has shown promise as a possible therapeutic for coeliac disease 1 Aspergillopepsin from Aspergillus niger has been shown to enhance the effects of EP B2 2 References edit Vora H McIntire J Kumar P Deshpande M Khosla C 2007 A scaleable manufacturing process for pro EP B2 a cysteine protease from barley indicated for celiac sprue Biotechnology and Bioengineering 98 1 177 185 doi 10 1002 bit 21423 PMID 17385743 S2CID 19581223 Ehren J Moron B Martin E Bethune M Gray G Khosla C El Shemy H A 2009 A food grade enzyme preparation with modest gluten detoxification properties PLOS ONE 4 7 e6313 Bibcode 2009PLoSO 4 6313E doi 10 1371 journal pone 0006313 PMC 2708912 PMID 19621078 Further reading editBethune M Ribka E Khosla C Sestak K Bozza P 2008 Transepithelial transport and enzymatic detoxification of gluten in gluten sensitive rhesus macaques PLOS ONE 3 3 e1857 Bibcode 2008PLoSO 3 1857B doi 10 1371 journal pone 0001857 PMC 2267209 PMID 18365012 Gass J Bethune M Siegel M Spencer A Khosla C 2007 Combination enzyme therapy for gastric digestion of dietary gluten in patients with celiac sprue Gastroenterology 133 2 472 480 doi 10 1053 j gastro 2007 05 028 PMID 17681168 Siegel M Bethune M Gass J Ehren J Xia J Johannsen A Stuge T Gray G Lee P Khosla C 2006 Rational design of combination enzyme therapy for celiac sprue Chemistry amp Biology 13 6 649 658 doi 10 1016 j chembiol 2006 04 009 PMID 16793522 Bethune M Strop P Tang Y Sollid L Khosla C 2006 Heterologous expression purification refolding and structural functional characterization of EP B2 a self activating barley cysteine endoprotease Chemistry amp Biology 13 6 637 647 doi 10 1016 j chembiol 2006 04 008 PMID 16793521 Gass J Vora H Bethune M Gray G Khosla C 2006 Effect of barley endoprotease EP B2 on gluten digestion in the intact rat The Journal of Pharmacology and Experimental Therapeutics 318 3 1178 1186 doi 10 1124 jpet 106 104315 PMID 16757540 S2CID 33662709 Portal nbsp Biology nbsp This enzyme related article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title EP B2 amp oldid 1170473122, wikipedia, wiki, book, books, library,
