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Apoptotic DNA fragmentation

October 30, 2023

Apoptotic DNA fragmentation is a key feature of apoptosis, a type of programmed cell death. Apoptosis is characterized by the activation of endogenous endonucleases, particularly the caspase-3 activated DNase (CAD),[1] with subsequent cleavage of nuclear DNA into internucleosomal fragments of roughly 180 base pairs (bp) and multiples thereof (360, 540 etc.). The apoptotic DNA fragmentation is being used as a marker of apoptosis and for identification of apoptotic cells either via the DNA laddering assay,[2] the TUNEL assay,[3][4] or the by detection of cells with fractional DNA content ("sub G1 cells") on DNA content frequency histograms e.g. as in the Nicoletti assay.[5][6]

Apoptotic DNA fragmentation, visualised by the DNA laddering assay (left). A 1 kb marker (middle) and control DNA (right) are included for comparison.

Mechanism edit

The enzyme responsible for apoptotic DNA fragmentation is the Caspase-Activated DNase (CAD). CAD is normally inhibited by another protein, the Inhibitor of Caspase Activated DNase (ICAD). During apoptosis, the apoptotic effector caspase, caspase-3, cleaves ICAD and thus causes CAD to become activated.[7]

 
A nucleosome, consisting of DNA (grey) wrapped around a histone tetramer (coloured). In apoptotic DNA fragmentation, the DNA is cleaved in the internucleosomal linker region, which is the part of the DNA not wrapped around the histones.

CAD cleaves DNA at internucleosomal linker sites between nucleosomes, protein-containing structures that occur in chromatin at ~180-bp intervals. This is because the DNA is normally tightly wrapped around histones, the core proteins of the nucleosomes. The linker sites are the only parts of the DNA strand that are exposed and thus accessible to CAD.

Degradation of nuclear DNA into nucleosomal units is one of the hallmarks of apoptotic cell death. It occurs in response to various apoptotic stimuli in a wide variety of cell types. Molecular characterization of this process identified a specific DNase (CAD, caspase-activated DNase) that cleaves chromosomal DNA in a caspase-dependent manner. CAD is synthesized with the help of ICAD (inhibitor of CAD), which works as a specific chaperone for CAD and is found complexed with ICAD in proliferating cells. When cells are induced to undergo apoptosis, caspase 3 cleaves ICAD to dissociate the CAD:ICAD complex, allowing CAD to cleave chromosomal DNA. Cells that lack ICAD or that express caspase-resistant mutant ICAD thus do not show DNA fragmentation during apoptosis, although they do exhibit some other features of apoptosis and die.

Even though much work has been performed on the analysis of apoptotic events, little information is available to link the timing of morphological features at the cell surface and in the nucleus to the biochemical degradation of DNA in the same cells. Apoptosis can be initiated by a myriad of different mechanisms in different cell types, and the kinetics of these events vary widely, from only a few minutes to several days depending on the cell system. The presence or absence of particular apoptotic event(s), including DNA fragmentation, depends on the "time window" at which the kinetic process of apoptosis is being investigated. Often this may complicate identification of apoptotic cells if cell populations are analyzed only at a single time point e.g. after induction of apoptosis.

Historical background edit

The discovery of the internucleosomal fragmentation of genomic DNA to regular repeating oligonucleosomal fragments generated by Ca/Mg-dependent endonuclease is accepted as one of the best-characterized biochemical markers of apoptosis (programmed cell death).

In 1970, Williamson described that cytoplasmic DNA isolated from mouse liver cells after culture was characterized by DNA fragments with a molecular weight consisting of multiples of 135 kDa. This finding was consistent with the hypothesis that these DNA fragments were a specific degradation product of nuclear DNA.[8] In 1972, Kerr, Wyllie, and Currie coined the term apoptosis and distinguished this type of cell death from necrosis based on morphological features.[9] In 1973, Hewish and Burgoyne, during the study of subchromatin structure, found that chromatin is accessible to the Ca++/Mg++ endonuclease, resulting in the formation of a digestion product with a regular series of molecular weight similar to the one previously described by Williamson (1970).[10] In 1974, Williams, Little, and Shipley, using cells exposed to widely differing types of trauma, found that during cell death, degraded DNA in "every case had a modal value of between 10(x6) and 10(x7) Dalton and cellular metabolism is required to produce degradation of DNA". However, this observation was without indication of "whether the incision attack on the DNA molecule was a random or rather at a particular site, that have structural or functional meaning".[11] In 1976, Scalka, Matyasova, and Cejkova described internucleosomal fragmentation of irradiated lymphoid chromatin DNA in vivo.[12]

Six years passed from 1972 to 1978/1980 until the discovery and evaluation of internucleosomal fragmentation of DNA during apoptotic cell death as a hallmark of apoptosis. Since 1972 (Kerr, Wyllie, and Currie[9]), it is accepted that glucocorticoid-induced death of lymphocytes is a form of apoptosis. In 1978, Zakharyan and Pogosyan presented a paper revealing that glucocorticoid-induced DNA degradation in rat lymphoid tissue, thymus, and spleen occurred in a specific pattern producing fragments of DNA that were electrophoretically similar to those observed after treatment of chromatin with microccoccal nuclease, which indicated internucleosomal cleavage pattern of DNA degradation occurred during apoptosis.[13][14] Thus, the first link between programmed cell death/apoptosis and internucleosomal fragmentation of chromatin DNA was discovered and soon became as a specific feature of apoptosis.

In 1980, Wyllie reported additional evidence for an internucleosomal DNA cleavage pattern as a specific feature of glucocorticoid-treated thymocytes undergoing apoptosis.[2] The internucleosomal DNA cleavage pattern was observed as a specific feature of apoptosis in 1978/1980 and has become a recognised hallmark of programmed cell death since then. In 1992 Gorczyca et al. [3] and Gavrieli et al.[4] independently described the DNA fragmentation assay based on the use of the terminal deoxynucleotidyl transferase (TUNEL) which become one of the standard methods to detect and identify apoptotic cells.

Detection assays edit

Flow cytometry is most frequently used to detect apoptotic DNA fragmentation.[15] Analysis of DNA content by flow cytometry can identify apoptotic cells with fragmented DNA as the cells with fractional DNA content, often called the sub-G1 cells. The flow-cytometric assay utilizing the fluorochrome acridine orange shows that DNA fragmentation within individual cells is discontinuous likely reflecting different levels of restriction in accessibility of DNA to DNase, by the supranucleosomal and nucleosomal levels of chromatin structure.[16] The presence of apoptotic "sub-G1cells" can also be detected in cells pre-fixed in ethanol but not after fixation in the crosslinking fixatives such as formaldehyde. The late-S and G2 apoptotic cells may not be detected with this approach because their fractional DNA content may overlap with that of the non-apoptotic G1 cells.[17] Treatment of cells with detergent, prior or concurrently with DNA fluorochrome, also reveals DNA fragmentation by virtue of the presence of the sub-G1 cells or cell fragments, as defined by Nicoletti et al.[5]

Apoptotic DNA fragmentation can also be detected by the TUNEL assay. The fluorochrome-based TUNEL assay applicable for flow cytometry, correlates the detection of DNA strand breaks with the cellular DNA content and thus with cell cycle-phase position. The avidin-peroxidase labeling TUNEL assay is applicable for light absorption microscopy. Many TUNEL-related kits are commercially available. Apoptotic DNA fragmentation is also analyzed using agarose gel electrophoresis to demonstrate a "ladder" pattern at ~180-BP intervals.[1] Necrosis, on the other hand, is usually characterized by random DNA fragmentation which forms a "smear" on agarose gels.

See also edit

References edit

  1. ^ Sakahira, H; Enari, M; Nagata, S (January 1998). "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis". Nature. 391 (6662): 96–9. Bibcode:1998Natur.391...96S. doi:10.1038/34214. PMID 9422513. S2CID 4329685.
  2. ^ a b Wyllie AH (1980-04-10). "Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation". Nature. 284 (5756): 555–556. Bibcode:1980Natur.284..555W. doi:10.1038/284555a0. ISSN 0028-0836. PMID 6245367. S2CID 4318802.
  3. ^ Gorczyca, W; Bruno, S; Darzynkiewicz, R; Gong, J; Darzynkiewicz, Z (Nov 1992). "DNA strand breaks occurring during apoptosis - their early insitu detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors". Int J Oncol. 1 (6): 639–48. doi:10.3892/ijo.1.6.639. PMID 21584593.
  4. ^ Gavrieli, Y.; Sherman, Y.; Ben-Sasson, S. A. (1992). "Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation". The Journal of Cell Biology. 119 (3): 493–501. doi:10.1083/jcb.119.3.493. PMC 2289665. PMID 1400587.
  5. ^ Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C (3 June 1991). "A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry". Journal of Immunological Methods. 139 (2): 271–279. doi:10.1016/0022-1759(91)90198-O. PMID 1710634.
  6. ^ Riccardi C, Nicoletti I (9 November 2006). "Analysis of apoptosis by propidium iodide staining and flow cytometry". Nature Protocols. 1 (3): 1458–1461. doi:10.1038/nprot.2006.238. PMID 17406435. S2CID 4469406.
  7. ^ Nagata, S.; Enari, M.; Sakahira, H.; Yokoyama, H.; Okawa, K.; Iwamatsu, A. (1998). "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD". Nature. 391 (6662): 43–50. Bibcode:1998Natur.391...43E. doi:10.1038/34112. PMID 9422506. S2CID 4407426.
  8. ^ Williamson, Robert (1970-07-14). "Properties of Rapidly Labeled Deoxyribonucleic Acid Fragments Isolated from the Cytoplasm of Primary Cultures of Embryonic Mouse Liver Cells". Journal of Molecular Biology. 51 (1): 157–168. doi:10.1016/0022-2836(70)90277-9. ISSN 0022-2836. PMID 5481278.
  9. ^ a b Kerr, John F. R.; Wyllie, Andrew; Currie, Alastair (August 1972). "Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics". British Journal of Cancer. 26 (4): 239–257. doi:10.1038/bjc.1972.33. ISSN 0007-0920. PMC 2008650. PMID 4561027.
  10. ^ Burgoyne, Leigh A.; Burgoyne, Leigh A. (1973-05-15). "Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease". Biochemical and Biophysical Research Communications. 52 (2): 504–510. doi:10.1016/0006-291X(73)90740-7. ISSN 0006-291X. PMID 4711166.
  11. ^ Williams, Jerry R.; Little, John B.; Shipley, William U. (1974-12-20). "Association of mammalian cell death with a specific endonucleolytic degradation of DNA". Nature. 252 (5485): 754–755. Bibcode:1974Natur.252..754W. doi:10.1038/252754a0. ISSN 0028-0836. PMID 4474604. S2CID 4181803.
  12. ^ Ceskova, M.; Matyásová, J; Cejková, M (1976-12-31). "DNA in chromatin of irradiated lymphoid tissues degrades in vivo into regular fragments". FEBS Letters. 72 (2): 271–274. doi:10.1016/0014-5793(76)80984-2. ISSN 0014-5793. PMID 16386038. S2CID 579849.
  13. ^ Zakharyan, R. A.; Pogosyan, R. G. (1978). "Glucocorticoid induction of the degradation of lymphocyte chromatin DNA into regularly repeating fragments in vivo". Doklady Akademii Nauk Armyanskoi SSR. 67 (2): 110–114. ISSN 0366-8606. CODEN: DANAAW, CAN 90:115643 AN 1979:115643 CAPLUS (Copyright 2003 ACS)
  14. ^ Chemical Abstracts v.90,1979;90:115643n p.112.
  15. ^ Gavanji S, Bakhtari A, Famurewa AC, Othman EM (January 2023). "Cytotoxic Activity of Herbal Medicines as Assessed in Vitro: A Review". Chemistry & Biodiversity. 20 (2): 3–27. doi:10.1002/cbdv.202201098. PMID 36595710. S2CID 255473013.
  16. ^ Kajstura, M; Halicka, HD; Pryjma, J; Darzynkiewicz, Z (2007). "Discontinuous fragmentation of nuclear DNA during apoptosis revealed by discrete "sub-G1" peaks on DNA content histograms". Cytometry A. 71 (3): 125–31. doi:10.1002/cyto.a.20357. PMID 17252584.
  17. ^ Wlodkowic, D; Telford, W; Skommer, J; Darzynkiewicz, Z (2011). "Apoptosis and Beyond: Cytometry in Studies of Programmed Cell Death". Recent Advances in Cytometry, Part B - Advances in Applications. pp. 55–98. doi:10.1016/B978-0-12-385493-3.00004-8. ISBN 9780123854933. PMC 3263828. PMID 21722800. {{cite book}}: |journal= ignored (help)

Further reading edit

  • Corcoran, G.; Fix, L.; Jones, D. P.; Moslen, M. T.; Nicotera, P.; Oberhammer, F. A.; Buttyan, R. (1994). "Apoptosis: Molecular Control Point in Toxicity". Toxicology and Applied Pharmacology. 128 (2): 169–181. doi:10.1006/taap.1994.1195. PMID 7940532.
  • Walker, P. R.; Pandey, S.; Sikorska, M. (1995). "Degradation of chromatin in apoptotic cells". Cell Death & Differentiation. 2 (2): 97–104. PMID 17180071.
  • Walker, P. R.; Sikorska, M. (1994). "Endonuclease activities, chromatin structure, and DNA degradation in apoptosis". Biochemistry and Cell Biology. 72 (11–12): 615–623. doi:10.1139/o94-081. PMID 7654335.
  • Pandey, S.; Walker, P. R.; Sikorska, M. (1994). "Separate pools of endonuclease activity are responsible for internucleosomal and high molecular mass DNA fragmentation during apoptosis". Biochemistry and Cell Biology. 72 (11–12): 625–629. doi:10.1139/o94-082. PMID 7654336.
  • Muñoz, E.; Marcos, A.; Unzaga, M. T. (1981). "Effect of protein deficiency on the lysosomal enzyme activities of the spleen and thymus of weanling rats". The Journal of Nutrition. 111 (12): 2133–2141. doi:10.1093/jn/111.12.2133. PMID 7310538.
  • Varela P, Marcos A, Rey de Viñas JL (1985). "Effect of cortisol treatment in pregnant rats, on cellular growth of progeny". IRCS Medical Science. 13: 412–413.

October 30, 2023
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This article may require cleanup to meet Wikipedia s quality standards No cleanup reason has been specified Please help improve this article if you can February 2012 Learn how and when to remove this template message Apoptotic DNA fragmentation is a key feature of apoptosis a type of programmed cell death Apoptosis is characterized by the activation of endogenous endonucleases particularly the caspase 3 activated DNase CAD 1 with subsequent cleavage of nuclear DNA into internucleosomal fragments of roughly 180 base pairs bp and multiples thereof 360 540 etc The apoptotic DNA fragmentation is being used as a marker of apoptosis and for identification of apoptotic cells either via the DNA laddering assay 2 the TUNEL assay 3 4 or the by detection of cells with fractional DNA content sub G1 cells on DNA content frequency histograms e g as in the Nicoletti assay 5 6 Apoptotic DNA fragmentation visualised by the DNA laddering assay left A 1 kb marker middle and control DNA right are included for comparison Contents 1 Mechanism 2 Historical background 3 Detection assays 4 See also 5 References 6 Further readingMechanism editThe enzyme responsible for apoptotic DNA fragmentation is the Caspase Activated DNase CAD CAD is normally inhibited by another protein the Inhibitor of Caspase Activated DNase ICAD During apoptosis the apoptotic effector caspase caspase 3 cleaves ICAD and thus causes CAD to become activated 7 nbsp A nucleosome consisting of DNA grey wrapped around a histone tetramer coloured In apoptotic DNA fragmentation the DNA is cleaved in the internucleosomal linker region which is the part of the DNA not wrapped around the histones CAD cleaves DNA at internucleosomal linker sites between nucleosomes protein containing structures that occur in chromatin at 180 bp intervals This is because the DNA is normally tightly wrapped around histones the core proteins of the nucleosomes The linker sites are the only parts of the DNA strand that are exposed and thus accessible to CAD Degradation of nuclear DNA into nucleosomal units is one of the hallmarks of apoptotic cell death It occurs in response to various apoptotic stimuli in a wide variety of cell types Molecular characterization of this process identified a specific DNase CAD caspase activated DNase that cleaves chromosomal DNA in a caspase dependent manner CAD is synthesized with the help of ICAD inhibitor of CAD which works as a specific chaperone for CAD and is found complexed with ICAD in proliferating cells When cells are induced to undergo apoptosis caspase 3 cleaves ICAD to dissociate the CAD ICAD complex allowing CAD to cleave chromosomal DNA Cells that lack ICAD or that express caspase resistant mutant ICAD thus do not show DNA fragmentation during apoptosis although they do exhibit some other features of apoptosis and die Even though much work has been performed on the analysis of apoptotic events little information is available to link the timing of morphological features at the cell surface and in the nucleus to the biochemical degradation of DNA in the same cells Apoptosis can be initiated by a myriad of different mechanisms in different cell types and the kinetics of these events vary widely from only a few minutes to several days depending on the cell system The presence or absence of particular apoptotic event s including DNA fragmentation depends on the time window at which the kinetic process of apoptosis is being investigated Often this may complicate identification of apoptotic cells if cell populations are analyzed only at a single time point e g after induction of apoptosis Historical background editThe discovery of the internucleosomal fragmentation of genomic DNA to regular repeating oligonucleosomal fragments generated by Ca Mg dependent endonuclease is accepted as one of the best characterized biochemical markers of apoptosis programmed cell death In 1970 Williamson described that cytoplasmic DNA isolated from mouse liver cells after culture was characterized by DNA fragments with a molecular weight consisting of multiples of 135 kDa This finding was consistent with the hypothesis that these DNA fragments were a specific degradation product of nuclear DNA 8 In 1972 Kerr Wyllie and Currie coined the term apoptosis and distinguished this type of cell death from necrosis based on morphological features 9 In 1973 Hewish and Burgoyne during the study of subchromatin structure found that chromatin is accessible to the Ca Mg endonuclease resulting in the formation of a digestion product with a regular series of molecular weight similar to the one previously described by Williamson 1970 10 In 1974 Williams Little and Shipley using cells exposed to widely differing types of trauma found that during cell death degraded DNA in every case had a modal value of between 10 x6 and 10 x7 Dalton and cellular metabolism is required to produce degradation of DNA However this observation was without indication of whether the incision attack on the DNA molecule was a random or rather at a particular site that have structural or functional meaning 11 In 1976 Scalka Matyasova and Cejkova described internucleosomal fragmentation of irradiated lymphoid chromatin DNA in vivo 12 Six years passed from 1972 to 1978 1980 until the discovery and evaluation of internucleosomal fragmentation of DNA during apoptotic cell death as a hallmark of apoptosis Since 1972 Kerr Wyllie and Currie 9 it is accepted that glucocorticoid induced death of lymphocytes is a form of apoptosis In 1978 Zakharyan and Pogosyan presented a paper revealing that glucocorticoid induced DNA degradation in rat lymphoid tissue thymus and spleen occurred in a specific pattern producing fragments of DNA that were electrophoretically similar to those observed after treatment of chromatin with microccoccal nuclease which indicated internucleosomal cleavage pattern of DNA degradation occurred during apoptosis 13 14 Thus the first link between programmed cell death apoptosis and internucleosomal fragmentation of chromatin DNA was discovered and soon became as a specific feature of apoptosis In 1980 Wyllie reported additional evidence for an internucleosomal DNA cleavage pattern as a specific feature of glucocorticoid treated thymocytes undergoing apoptosis 2 The internucleosomal DNA cleavage pattern was observed as a specific feature of apoptosis in 1978 1980 and has become a recognised hallmark of programmed cell death since then In 1992 Gorczyca et al 3 and Gavrieli et al 4 independently described the DNA fragmentation assay based on the use of the terminal deoxynucleotidyl transferase TUNEL which become one of the standard methods to detect and identify apoptotic cells Detection assays editFlow cytometry is most frequently used to detect apoptotic DNA fragmentation 15 Analysis of DNA content by flow cytometry can identify apoptotic cells with fragmented DNA as the cells with fractional DNA content often called the sub G1 cells The flow cytometric assay utilizing the fluorochrome acridine orange shows that DNA fragmentation within individual cells is discontinuous likely reflecting different levels of restriction in accessibility of DNA to DNase by the supranucleosomal and nucleosomal levels of chromatin structure 16 The presence of apoptotic sub G1cells can also be detected in cells pre fixed in ethanol but not after fixation in the crosslinking fixatives such as formaldehyde The late S and G2 apoptotic cells may not be detected with this approach because their fractional DNA content may overlap with that of the non apoptotic G1 cells 17 Treatment of cells with detergent prior or concurrently with DNA fluorochrome also reveals DNA fragmentation by virtue of the presence of the sub G1 cells or cell fragments as defined by Nicoletti et al 5 Apoptotic DNA fragmentation can also be detected by the TUNEL assay The fluorochrome based TUNEL assay applicable for flow cytometry correlates the detection of DNA strand breaks with the cellular DNA content and thus with cell cycle phase position The avidin peroxidase labeling TUNEL assay is applicable for light absorption microscopy Many TUNEL related kits are commercially available Apoptotic DNA fragmentation is also analyzed using agarose gel electrophoresis to demonstrate a ladder pattern at 180 BP intervals 1 Necrosis on the other hand is usually characterized by random DNA fragmentation which forms a smear on agarose gels See also editCaspase activated DNase DNA ladderingReferences edit Sakahira H Enari M Nagata S January 1998 Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis Nature 391 6662 96 9 Bibcode 1998Natur 391 96S doi 10 1038 34214 PMID 9422513 S2CID 4329685 a b Wyllie AH 1980 04 10 Glucocorticoid induced thymocyte apoptosis is associated with endogenous endonuclease activation Nature 284 5756 555 556 Bibcode 1980Natur 284 555W doi 10 1038 284555a0 ISSN 0028 0836 PMID 6245367 S2CID 4318802 Gorczyca W Bruno S Darzynkiewicz R Gong J Darzynkiewicz Z Nov 1992 DNA strand breaks occurring during apoptosis their early insitu detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors Int J Oncol 1 6 639 48 doi 10 3892 ijo 1 6 639 PMID 21584593 Gavrieli Y Sherman Y Ben Sasson S A 1992 Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation The Journal of Cell Biology 119 3 493 501 doi 10 1083 jcb 119 3 493 PMC 2289665 PMID 1400587 Nicoletti I Migliorati G Pagliacci MC Grignani F Riccardi C 3 June 1991 A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry Journal of Immunological Methods 139 2 271 279 doi 10 1016 0022 1759 91 90198 O PMID 1710634 Riccardi C Nicoletti I 9 November 2006 Analysis of apoptosis by propidium iodide staining and flow cytometry Nature Protocols 1 3 1458 1461 doi 10 1038 nprot 2006 238 PMID 17406435 S2CID 4469406 Nagata S Enari M Sakahira H Yokoyama H Okawa K Iwamatsu A 1998 A caspase activated DNase that degrades DNA during apoptosis and its inhibitor ICAD Nature 391 6662 43 50 Bibcode 1998Natur 391 43E doi 10 1038 34112 PMID 9422506 S2CID 4407426 Williamson Robert 1970 07 14 Properties of Rapidly Labeled Deoxyribonucleic Acid Fragments Isolated from the Cytoplasm of Primary Cultures of Embryonic Mouse Liver Cells Journal of Molecular Biology 51 1 157 168 doi 10 1016 0022 2836 70 90277 9 ISSN 0022 2836 PMID 5481278 a b Kerr John F R Wyllie Andrew Currie Alastair August 1972 Apoptosis A Basic Biological Phenomenon with Wide ranging Implications in Tissue Kinetics British Journal of Cancer 26 4 239 257 doi 10 1038 bjc 1972 33 ISSN 0007 0920 PMC 2008650 PMID 4561027 Burgoyne Leigh A Burgoyne Leigh A 1973 05 15 Chromatin sub structure The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease Biochemical and Biophysical Research Communications 52 2 504 510 doi 10 1016 0006 291X 73 90740 7 ISSN 0006 291X PMID 4711166 Williams Jerry R Little John B Shipley William U 1974 12 20 Association of mammalian cell death with a specific endonucleolytic degradation of DNA Nature 252 5485 754 755 Bibcode 1974Natur 252 754W doi 10 1038 252754a0 ISSN 0028 0836 PMID 4474604 S2CID 4181803 Ceskova M Matyasova J Cejkova M 1976 12 31 DNA in chromatin of irradiated lymphoid tissues degrades in vivo into regular fragments FEBS Letters 72 2 271 274 doi 10 1016 0014 5793 76 80984 2 ISSN 0014 5793 PMID 16386038 S2CID 579849 Zakharyan R A Pogosyan R G 1978 Glucocorticoid induction of the degradation of lymphocyte chromatin DNA into regularly repeating fragments in vivo Doklady Akademii Nauk Armyanskoi SSR 67 2 110 114 ISSN 0366 8606 CODEN DANAAW CAN 90 115643 AN 1979 115643 CAPLUS Copyright 2003 ACS Chemical Abstracts v 90 1979 90 115643n p 112 Gavanji S Bakhtari A Famurewa AC Othman EM January 2023 Cytotoxic Activity of Herbal Medicines as Assessed in Vitro A Review Chemistry amp Biodiversity 20 2 3 27 doi 10 1002 cbdv 202201098 PMID 36595710 S2CID 255473013 Kajstura M Halicka HD Pryjma J Darzynkiewicz Z 2007 Discontinuous fragmentation of nuclear DNA during apoptosis revealed by discrete sub G1 peaks on DNA content histograms Cytometry A 71 3 125 31 doi 10 1002 cyto a 20357 PMID 17252584 Wlodkowic D Telford W Skommer J Darzynkiewicz Z 2011 Apoptosis and Beyond Cytometry in Studies of Programmed Cell Death Recent Advances in Cytometry Part B Advances in Applications pp 55 98 doi 10 1016 B978 0 12 385493 3 00004 8 ISBN 9780123854933 PMC 3263828 PMID 21722800 a href Template Cite book html title Template Cite book cite book a journal ignored help Gold R Schmied M Rothe G Zischler H Breitschopf H Wekerle H Lassmann H July 1993 Detection of DNA fragmentation in apoptosis application of in situ nick translation to cell culture systems and tissue sections Journal of Histochemistry amp Cytochemistry 41 7 1023 1030 doi 10 1177 41 7 8515045 ISSN 0022 1554 PMID 8515045 Collins JA Schandi CA Young KK Vesely J Willingham MC July 1997 Major DNA fragmentation is a late event in apoptosis Journal of Histochemistry amp Cytochemistry 45 7 923 934 doi 10 1177 002215549704500702 ISSN 0022 1554 PMID 9212818 Nagata Shigekazu 2000 04 10 Apoptotic DNA fragmentation Experimental Cell Research 256 1 12 18 doi 10 1006 excr 2000 4834 ISSN 0014 4827 PMID 10739646 Further reading editCorcoran G Fix L Jones D P Moslen M T Nicotera P Oberhammer F A Buttyan R 1994 Apoptosis Molecular Control Point in Toxicity Toxicology and Applied Pharmacology 128 2 169 181 doi 10 1006 taap 1994 1195 PMID 7940532 Walker P R Pandey S Sikorska M 1995 Degradation of chromatin in apoptotic cells Cell Death amp Differentiation 2 2 97 104 PMID 17180071 Walker P R Sikorska M 1994 Endonuclease activities chromatin structure and DNA degradation in apoptosis Biochemistry and Cell Biology 72 11 12 615 623 doi 10 1139 o94 081 PMID 7654335 Pandey S Walker P R Sikorska M 1994 Separate pools of endonuclease activity are responsible for internucleosomal and high molecular mass DNA fragmentation during apoptosis Biochemistry and Cell Biology 72 11 12 625 629 doi 10 1139 o94 082 PMID 7654336 Munoz E Marcos A Unzaga M T 1981 Effect of protein deficiency on the lysosomal enzyme activities of the spleen and thymus of weanling rats The Journal of Nutrition 111 12 2133 2141 doi 10 1093 jn 111 12 2133 PMID 7310538 Varela P Marcos A Rey de Vinas JL 1985 Effect of cortisol treatment in pregnant rats on cellular growth of progeny IRCS Medical Science 13 412 413 Retrieved from https en wikipedia org w index php title Apoptotic DNA fragmentation amp oldid 1173882391, wikipedia, wiki, book, books, library,

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