Acyl-CoA thioesterase 9

Acyl-CoA thioesterase 9 is a protein that is encoded by the human ACOT9 gene. It is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that hydrolyze Coenzyme A esters. There is no known function, however it has been shown to act as a long-chain thioesterase at low concentrations, and a short-chain thioesterase at high concentrations.^[5]

ACOT9
Identifiers
Aliases	ACOT9, ACATE2, MT-ACT48, MTACT48, CGI-16, Acyl-CoA thioesterase 9
External IDs	OMIM: 300862 MGI: 1928939 HomoloGene: 8206 GeneCards: ACOT9
Gene location (Human) Chr. X chromosome (human)^[1] Band Xp22.11 Start 23,701,055 bp^[1] End 23,766,475 bp^[1]
Gene location (Mouse) Chr. X chromosome (mouse)^[2] Band X|X F3 Start 154,045,439 bp^[2] End 154,080,650 bp^[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte Achilles tendon left ventricle monocyte ascending aorta gastric mucosa right ventricle right coronary artery upper lobe of left lung right lung Top expressed in hair follicle facial motor nucleus cornea atrioventricular valve endocardial cushion conjunctival fornix trigeminal ganglion brown adipose tissue carotid body proximal tubule More reference expression data BioGPS n/a
Gene ontology Molecular function acetyl-CoA hydrolase activity hydrolase activity carboxylic ester hydrolase activity acyl-CoA hydrolase activity Cellular component mitochondrial matrix mitochondrion Biological process acyl-CoA metabolic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 23597 56360 Ensembl ENSG00000123130 ENSMUSG00000025287 UniProt Q9Y305 Q9R0X4 RefSeq (mRNA) NM_001033583 NM_001037171 NM_001330259 NM_012332 NM_019736 NM_001313718 RefSeq (protein) NP_001028755 NP_001032248 NP_001317188 NP_001300647 NP_062710 Location (UCSC) Chr X: 23.7 – 23.77 Mb Chr X: 154.05 – 154.08 Mb PubMed search ^[3] ^[4]
Wikidata
View/Edit Human View/Edit Mouse

Gene

 

Depiction of ACOT9 gene

Locus

The ACOT9 gene is located at p22.11 on chromosome X. Located on the minus strand of the chromosome, the start is at 23,721,777 bp and the end is at 23,761,407 bp, which is a span of 39,631 base pairs.^[6]

 

ACOT9 location on the human chromosome X

Aliases

ACOT9 gene is known primarily for encoding the Acyl-CoA thioesterase 9 protein. Other, less commonly used names for the gene are ACATE2,^[7] and MT-ACT48.^[8]

Function

The protein encoded by the ACOT9 gene is part of a family of Acyl-CoA thioesterases, which catalyze the hydrolysis of various Coenzyme A esters of various molecules to the free acid plus CoA. These enzymes have also been referred to in the literature as acyl-CoA hydrolases, acyl-CoA thioester hydrolases, and palmitoyl-CoA hydrolases. The reaction carried out by these enzymes is as follows:

CoA ester + H₂O → free acid + coenzyme A

These enzymes use the same substrates as long-chain acyl-CoA synthetases, but have a unique purpose in that they generate the free acid and CoA, as opposed to long-chain acyl-CoA synthetases, which ligate fatty acids to CoA, to produce the CoA ester.^[9] The role of the ACOT- family of enzymes is not well understood; however, it has been suggested that they play a crucial role in regulating the intracellular levels of CoA esters, Coenzyme A, and free fatty acids. Recent studies have shown that Acyl-CoA esters have many more functions than simply an energy source. These functions include allosteric regulation of enzymes such as acetyl-CoA carboxylase,^[10] hexokinase IV,^[11] and the citrate condensing enzyme. Long-chain acyl-CoAs also regulate opening of ATP-sensitive potassium channels and activation of Calcium ATPases, thereby regulating insulin secretion.^[12] A number of other cellular events are also mediated via acyl-CoAs, for example signal transduction through protein kinase C, inhibition of retinoic acid-induced apoptosis, and involvement in budding and fusion of the endomembrane system.^[13][14][15] Acyl-CoAs also mediate protein targeting to various membranes and regulation of G Protein α subunits, because they are substrates for protein acylation.^[16] In the mitochondria, acyl-CoA esters are involved in the acylation of mitochondrial NAD+ dependent dehydrogenases; because these enzymes are responsible for amino acid catabolism, this acylation renders the whole process inactive. This mechanism may provide metabolic crosstalk and act to regulate the NADH/NAD+ ratio in order to maintain optimal mitochondrial beta oxidation of fatty acids.^[17] The role of CoA esters in lipid metabolism and numerous other intracellular processes are well defined, and thus it is hypothesized that ACOT- enzymes play a role in modulating the processes these metabolites are involved in.^[18]

Homology/Evolution

 

Divergence of Sequence Identity (%) vs. Time (MYA) in ACOT9

Orthologs

There are many orthologs of ACOT9, the house mouse (Mus musculus) being one of the most similar, where the ACOT9 gene is found at 72.38cM on chromosome X.^[19] The range of orthologs extends to mammals, birds, amphibians, anamorphic fungi, and others.^{[citation needed]}

Sequence number	Genus and species	Common name	Date of divergence (MYA)	Accession number	Sequence length	Sequence identity	Sequence similarity	Notes
1	Homo sapiens	Human	0	NP_001028755.2	439	100%	100%	Human
2	Mus musculus	House mouse	91	NP_062710.2	439	83%	90%	Rodent
3	Pteropus alecto	Black flying fox	97.4	XP_006911668.1	480	81%	91%	Bat
4	Gallus gallus	Chicken	324.5	NP_001012841.1	425	69%	87%	Bird
5	Pseudopodoces humilis	Ground tit	324.5	XP_005516751.1	417	68%	85%	Bird
6	Columba livia	Rock dove	324.5	XP_005503782.1	402	67%	86%	Bird
7	Geospiza fortis	Medium ground finch	324.5	XP_005424946.1	417	67%	85%	Bird
8	Pelodiscus sinensis	Chinese soft shelled turtle	324.5	XP_006112565.1	439	67%	85%	Reptile
9	Xenopus tropicalis	Western clawed frog	361.2	AAI61600.1	418	65%	82%	Amphibian
10	Danio rerio	Zebrafish	454.6	AAI59216.1	434	60%	80%	Fish
11	Ceratitis capitata	Mediterranean fruit fly	910	JAB97119.1	433	32%	58%	Insect
12	Glarea lozoyensis 74030	Anamorphic fungus	1368	EHL00310.1	350	24%	47%	Fungus

 

Conservation of ACOT9 gene between H. sapiens, G. lozoyensis, and C. capitata

Paralogs

In mice, which is one of the closest orthologs, ACOT10 is a known paralog of the ACOT9 gene.^[20]

Expression

 

ACOT9 expression chart

Expression of the ACOT9 is ubiquitous throughout the tissues in humans. Tissues with a value of over 500 in the large-scale analysis of the human transcriptome were the globus pallidus and colorectal adenocarcinoma.^[21] The expressed sequence tag (or EST) abundance profile also shows ubiquitous/near ubiquitous, expression throughout human tissues.^[22]

Transcription factors

There are numerous transcription factors throughout the ACOT9 promoter sequence. Some of the notable factors are heat shock factors and transcription factor II B (TFIIB) recognition elements.^{[citation needed]}

Transcription factor	Start	End	Strand	Sequence
X gene core promoter element 1	683	693	-	ggGCGGgaccg
Doublesex and mab-3 related transcription factor 1	81	101	+	tttttttgagacaTTGTctcc
cAMP-responsive element binding protein 1	491	511	-	agggcgTGACgtcgagaagag
Sp4 transcription factor	660	676	-	ccagggGGCGtggccgc
Stimulating protein 1, ubiquitous zinc finger transcription factor	682	698	-	tccggGGGCgggaccgc
Heat shock factor 1	24	48	+	caggactaaactAGAAtctccagcc
E2F transcription factor 2	808	824	+	ccatcGCGCgcacggca
Nuclear factor of activated T-cells 5	380	398	+	tttGGAAagttgcccagga
ZF5 POZ domain zinc finger, zinc finger protein 161 (secondary DNA binding preference)	811	825	+	tcgCGCGcacggcag
B-cell-specific activator protein	678	706	-	cagcggtgtccgggGGCGggaccgcggcg
Pax-6 paired domain binding site	54	72	+	gtctcAAGCatcagttttt
ZF5 POZ domain zinc finger, zinc finger protein 161 (secondary DNA binding preference)	651	665	-	ggcCGCGctgtgccg
Pax-6 paired domain binding site	758	776	+	ttttaTCGCctcagtttcc
Mammalian C-type LTR TATA box	751	767	-	ggcgaTAAAagacgcac
Nuclear factor Y (Y-box binding factor)	624	638	+	cccgCCAAtgaacgg
Transcription factor II B (TFIIB) recognition element	356	362	+	ccgCGCC
Transcription factor II B (TFIIB) recognition element	440	446	-	ccgCGCC
Transcription factor II B (TFIIB) recognition element	734	740	-	ccgCGCC
Nuclear factor Y (Y-box binding factor)	581	595	-	ccacTCAAtcagttg
CCAAT/enhancer binding protein alpha	529	543	-	tcggttgaGTAAacg

Secondary structure

There are two regions in the ACOT9 gene sequence that are labeled as BFIT (Brown Fat Inducible Thioesterase) and BACH (Brain Acyl CoA Hydrolase) regions. These regions are part of a hotdog fold superfamily, which has been found to be used in a variety of cell roles.^[23] Predictions show there to be various alpha-helices throughout the structure,^[24] suggesting it is a transmembrane protein.

Interactions

A mitochondrial cleavage site can be found at amino acid 30 in the ACOT9 sequence, and the probability of export to the mitochondria is 0.9374.^[25] The Acyl-CoA thioesterase 9 protein is estimated to be 60.9% mitochondrial, 21.7% cytoplasmic, 8.7% nuclear, 4.3% in the plasma membrane, and 4.3% in the endoplasmic reticulum.^[26]

The ACOT9 protein has been found to interact with the following proteins either experimentally or through co-expression:^[27]

• C1orf151
• MCTS1
• C1GALT1C1
• FBXW12
• RLIM
• RPS6KA3
• RAB9A
• UBC
• CXorf26
• HCCS

References

1. GRCh38: Ensembl release 89: ENSG00000123130 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000025287 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Tillander V, Arvidsson Nordström E, Reilly J, Strozyk M, Van Veldhoven PP, Hunt MC, Alexson SE (Mar 2014). "Acyl-CoA thioesterase 9 (ACOT9) in mouse may provide a novel link between fatty acid and amino acid metabolism in mitochondria". Cellular and Molecular Life Sciences. 71 (5): 933–48. doi:10.1007/s00018-013-1422-1. hdl:10616/41794. PMID 23864032. S2CID 18767370.
6. Kent WJ, Sugnet CW, Furey TS, Roskin KM, Pringle TH, Zahler AM, Haussler D (June 12, 2002). "Human Feb. 2009 (GRCh37/hg19) Assembly". The human genome browser at UCSC. UCSC Genome Bioinformatics. Retrieved March 12, 2014.
7. Gu J, MacHugh DE, McGivney BA, Park SD, Katz LM, Hill EW (Nov 2010). "Association of sequence variants in CKM (creatine kinase, muscle) and COX4I2 (cytochrome c oxidase, subunit 4, isoform 2) genes with racing performance in Thoroughbred horses". Equine Veterinary Journal. Supplement. 42 (38): 569–75. doi:10.1111/j.2042-3306.2010.00181.x. PMID 21059062.
8. Poupon V, Bègue B, Gagnon J, Dautry-Varsat A, Cerf-Bensussan N, Benmerah A (Jul 1999). "Molecular cloning and characterization of MT-ACT48, a novel mitochondrial acyl-CoA thioesterase". The Journal of Biological Chemistry. 274 (27): 19188–94. doi:10.1074/jbc.274.27.19188. PMID 10383425.
9. Mashek DG, Bornfeldt KE, Coleman RA, Berger J, Bernlohr DA, Black P, DiRusso CC, Farber SA, Guo W, Hashimoto N, Khodiyar V, Kuypers FA, Maltais LJ, Nebert DW, Renieri A, Schaffer JE, Stahl A, Watkins PA, Vasiliou V, Yamamoto TT (Oct 2004). "Revised nomenclature for the mammalian long-chain acyl-CoA synthetase gene family". Journal of Lipid Research. 45 (10): 1958–61. doi:10.1194/jlr.e400002-jlr200. PMID 15292367.
10. Ogiwara H, Tanabe T, Nikawa J, Numa S (Aug 1978). "Inhibition of rat-liver acetyl-coenzyme-A carboxylase by palmitoyl-coenzyme A. Formation of equimolar enzyme-inhibitor complex". European Journal of Biochemistry. 89 (1): 33–41. doi:10.1111/j.1432-1033.1978.tb20893.x. PMID 29756.
11. Srere PA (Dec 1965). "Palmityl-coenzyme A inhibition of the citrate-condensing enzyme". Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 106 (3): 445–55. doi:10.1016/0005-2760(65)90061-5. PMID 5881327.
12. Gribble FM, Proks P, Corkey BE, Ashcroft FM (Oct 1998). "Mechanism of cloned ATP-sensitive potassium channel activation by oleoyl-CoA". The Journal of Biological Chemistry. 273 (41): 26383–7. doi:10.1074/jbc.273.41.26383. PMID 9756869.
13. Nishizuka Y (Apr 1995). "Protein kinase C and lipid signaling for sustained cellular responses". FASEB Journal. 9 (7): 484–96. doi:10.1096/fasebj.9.7.7737456. PMID 7737456. S2CID 31065063.
14. Glick BS, Rothman JE (1987). "Possible role for fatty acyl-coenzyme A in intracellular protein transport". Nature. 326 (6110): 309–12. Bibcode:1987Natur.326..309G. doi:10.1038/326309a0. PMID 3821906. S2CID 4306469.
15. Wan YJ, Cai Y, Cowan C, Magee TR (Jun 2000). "Fatty acyl-CoAs inhibit retinoic acid-induced apoptosis in Hep3B cells". Cancer Letters. 154 (1): 19–27. doi:10.1016/s0304-3835(00)00341-4. PMID 10799735.
16. Duncan JA, Gilman AG (Jun 1998). "A cytoplasmic acyl-protein thioesterase that removes palmitate from G protein alpha subunits and p21(RAS)". The Journal of Biological Chemistry. 273 (25): 15830–7. doi:10.1074/jbc.273.25.15830. PMID 9624183.
17. Berthiaume L, Deichaite I, Peseckis S, Resh MD (Mar 1994). "Regulation of enzymatic activity by active site fatty acylation. A new role for long chain fatty acid acylation of proteins". The Journal of Biological Chemistry. 269 (9): 6498–505. doi:10.1016/S0021-9258(17)37399-4. PMID 8120000.
18. Hunt MC, Alexson SE (Mar 2002). "The role Acyl-CoA thioesterases play in mediating intracellular lipid metabolism". Progress in Lipid Research. 41 (2): 99–130. doi:10.1016/s0163-7827(01)00017-0. PMID 11755680.
19. "ACOT9 gene detail". Mouse Genome Database. Retrieved 2014-06-19.
20. "Gene: Acot9". Ensembl release 75.
21. "Large-scale analysis of the human transcriptome (HG-U133A)". National Center for Biotechnology Information. Retrieved 10 May 2014.
22. "EST Profile Hs.298885 - ACOT9: Acyl-CoA thioesterase 9". Retrieved 10 May 2014.
23. Dillon SC, Bateman A (Aug 2004). "The Hotdog fold: wrapping up a superfamily of thioesterases and dehydratases". BMC Bioinformatics. 5: 109. doi:10.1186/1471-2105-5-109. PMC 516016. PMID 15307895.
24. "SDSC Biology WorkBench 3.2 Pele Program". ^{[dead link]}
25. Claros MG, Vincens P (Nov 1996). "Computational method to predict mitochondrially imported proteins and their targeting sequences". European Journal of Biochemistry. 241 (3): 779–86. doi:10.1111/j.1432-1033.1996.00779.x. PMID 8944766.
26. "PSORTII Prediction Tool".^{[verification needed]}
27. Jensen LJ, Kuhn M, Stark M, Chaffron S, Creevey C, Muller J, Doerks T, Julien P, Roth A, Simonovic M, Bork P, von Mering C (Jan 2009). "STRING 8--a global view on proteins and their functional interactions in 630 organisms". Nucleic Acids Research. 37 (Database issue): D412–6. doi:10.1093/nar/gkn760. PMC 2686466. PMID 18940858.

External links

• Human ACOT9 genome location and ACOT9 gene details page in the UCSC Genome Browser.

Further reading

• Mulkearns EE, Cooper JA (Apr 2012). "FCH domain only-2 organizes clathrin-coated structures and interacts with Disabled-2 for low-density lipoprotein receptor endocytosis". Molecular Biology of the Cell. 23 (7): 1330–42. doi:10.1091/mbc.E11-09-0812. PMC 3315808. PMID 22323290.
• Hunt MC, Yamada J, Maltais LJ, Wright MW, Podesta EJ, Alexson SE (Sep 2005). "A revised nomenclature for mammalian acyl-CoA thioesterases/hydrolases". Journal of Lipid Research. 46 (9): 2029–32. doi:10.1194/jlr.E500003-JLR200. PMID 16103133.
• Alkhaja AK, Jans DC, Nikolov M, Vukotic M, Lytovchenko O, Ludewig F, Schliebs W, Riedel D, Urlaub H, Jakobs S, Deckers M (Jan 2012). "MINOS1 is a conserved component of mitofilin complexes and required for mitochondrial function and cristae organization" (PDF). Molecular Biology of the Cell. 23 (2): 247–57. doi:10.1091/mbc.E11-09-0774. PMC 3258170. PMID 22114354.
• Poupon V, Bègue B, Gagnon J, Dautry-Varsat A, Cerf-Bensussan N, Benmerah A (Jul 1999). "Molecular cloning and characterization of MT-ACT48, a novel mitochondrial acyl-CoA thioesterase". The Journal of Biological Chemistry. 274 (27): 19188–94. doi:10.1074/jbc.274.27.19188. PMID 10383425.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.