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Verhoeff's stain

February 28, 2023

Verhoeff's stain, also known as Verhoeff's elastic stain (VEG) or Verhoeff–Van Gieson stain (VVG),[1] is a staining protocol used in histology, developed by American ophthalmic surgeon and pathologist Frederick Herman Verhoeff (1874–1968) in 1908.[2] The formulation is used to demonstrate normal or pathologic elastic fibers.

Verhoeff's stain forms a variety of cationic, anionic and non-ionic bonds with elastin, the main constituent of elastic fiber tissue.[2] Elastin has a strong affinity for the iron-hematoxylin complex formed by the reagents in the stain and will hence retain dye longer than other tissue elements. This allows elastin to remain stained, while remaining tissue elements are decolorized. Sodium thiosulfate is used to remove excess iodine and a counterstain (most often Van Gieson's stain) is used to contrast the principal stain.[3] Elastic fibers and cell nuclei are stained black, collagen fibers are stained red, and other tissue elements including cytoplasm are stained yellow.

Reagents

Verhoeff's stain is a combination of the following reagents:[3]

Variants

A common variant is the Masson trichrome and Verhoeff stain, which combines the Masson's trichrome stain and Verhoeff stain.[4] It is sometimes just referred to as a Masson trichrome.[citation needed] This combination is useful for the examination of blood vessels; the Verhoeff stain highlights elastin (black) and allows one to easily differentiate small arteries (which typically have two elastic laminae) and veins (which have one elastic lamina).

References

  1. ^ "Verhoeff-Van Gieson (VVG) Staining Protocol for Elastic Fibers". ICH World. Retrieved 2 January 2013.
  2. ^ a b Puchtler, H; Waldrop, FS (Aug 1979). "On the mechanism of Verhoeff's elastica stain: a convenient stain for myelin sheaths". Histochemistry. 62 (3): 233–247. doi:10.1007/BF00508352. PMID 90671. S2CID 8869007. Retrieved 2 January 2013.
  3. ^ a b "Verhoeff's Elastic Stain". The Visible Mouse Project. Mallory, Sheehan and Hrapchak in: Histotechnology - A Self-Instructional Text. ASCP Press, Chicago 1990. UC Davis. Retrieved 2 January 2013.{{cite web}}: CS1 maint: others (link)
  4. ^ . Virginia Tech. Archived from the original on 2011-09-27. Retrieved 2 January 2013.
 
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February 28, 2023
verhoeff, stain, also, known, verhoeff, elastic, stain, verhoeff, gieson, stain, staining, protocol, used, histology, developed, american, ophthalmic, surgeon, pathologist, frederick, herman, verhoeff, 1874, 1968, 1908, formulation, used, demonstrate, normal, . Verhoeff s stain also known as Verhoeff s elastic stain VEG or Verhoeff Van Gieson stain VVG 1 is a staining protocol used in histology developed by American ophthalmic surgeon and pathologist Frederick Herman Verhoeff 1874 1968 in 1908 2 The formulation is used to demonstrate normal or pathologic elastic fibers Verhoeff s stain forms a variety of cationic anionic and non ionic bonds with elastin the main constituent of elastic fiber tissue 2 Elastin has a strong affinity for the iron hematoxylin complex formed by the reagents in the stain and will hence retain dye longer than other tissue elements This allows elastin to remain stained while remaining tissue elements are decolorized Sodium thiosulfate is used to remove excess iodine and a counterstain most often Van Gieson s stain is used to contrast the principal stain 3 Elastic fibers and cell nuclei are stained black collagen fibers are stained red and other tissue elements including cytoplasm are stained yellow Reagents EditVerhoeff s stain is a combination of the following reagents 3 Haematoxylin Iron III chloride Lugol s iodine Van Gieson s stain Acid fuchsin Picric acid Sodium thiosulfateVariants EditA common variant is the Masson trichrome and Verhoeff stain which combines the Masson s trichrome stain and Verhoeff stain 4 It is sometimes just referred to as a Masson trichrome citation needed This combination is useful for the examination of blood vessels the Verhoeff stain highlights elastin black and allows one to easily differentiate small arteries which typically have two elastic laminae and veins which have one elastic lamina References Edit Verhoeff Van Gieson VVG Staining Protocol for Elastic Fibers ICH World Retrieved 2 January 2013 a b Puchtler H Waldrop FS Aug 1979 On the mechanism of Verhoeff s elastica stain a convenient stain for myelin sheaths Histochemistry 62 3 233 247 doi 10 1007 BF00508352 PMID 90671 S2CID 8869007 Retrieved 2 January 2013 a b Verhoeff s Elastic Stain The Visible Mouse Project Mallory Sheehan and Hrapchak in Histotechnology A Self Instructional Text ASCP Press Chicago 1990 UC Davis Retrieved 2 January 2013 a href Template Cite web html title Template Cite web cite web a CS1 maint others link Masson Trichrome amp Verhoeff Stain Virginia Tech Archived from the original on 2011 09 27 Retrieved 2 January 2013 Wikimedia Commons has media related to Verhoeff s stain Retrieved from https en wikipedia org w index php title Verhoeff 27s stain amp oldid 1117102867, wikipedia, wiki, book, books, library,

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