Formally known as U-87 MG (abbreviation for Uppsala 87 Malignant Glioma), the U87 cell line has an epithelialmorphology and was obtained from a 44-year-old female patient in 1966 at Uppsala University. The cell line was thought to be deposited at the Memorial Sloan Kettering Cancer Center in 1973, after which the ATCC obtained it in 1982.[2] However, in 2016 the commonly used version of U87MG (from the ATCC) was found to be non-identical to its patient of origin.[3] Analysis of DNA profile and mtDNA position suggest that the Uppsala U-87 MG line is authentic to the original tumor tissue while the ATCC U-87 MG line is not; The ATCC line is likely a bona fide human glioblastoma cell line of unknown origin.[3] The entire sequence of the genome of U-87 MG has been published in PLoS Genetics.[1] U-87 MG can be obtained from the American Type Culture Collection (ATCC) where it is known by the accession number HTB-14 and it is reported that the cell line comes from a male patient of unknown age.[4]
Growth conditionsedit
U87 growth media is generally made with Eagle's minimum essential medium + 10% FBS + 100 U/mL penicillin + 100 µg/mL streptomycin.[4] It is propagated at 37 °C in a 5% carbon dioxide atmosphere.
Referencesedit
^ abClark, MJ; Homer, N; O'Connor, BD; et al. (January 2010). "U87MG decoded: the genomic sequence of a cytogenetically aberrant human cancer cell line". PLOS Genet. 6 (1): e1000832. doi:10.1371/journal.pgen.1000832. PMC2813426. PMID 20126413.
^Dolgin, Ellie (31 August 2016). "Venerable brain-cancer cell line faces identity crisis". Nature. 537 (7619): 149–150. Bibcode:2016Natur.537..149D. doi:10.1038/nature.2016.20515. PMID 27604929. S2CID 4460070. Retrieved 3 May 2018.
^ abAllen, M; Bjerke, M; Edlund, H; Nelander, S; Westermark, B (Aug 2016). "Origin of the U87MG glioma cell line: Good news and bad news". Sci Transl Med. 8 (354): 354re3. doi:10.1126/scitranslmed.aaf6853. PMID 27582061. S2CID 4577520.
cell, biology, human, glioblastoma, cell, line, that, commonly, used, brain, cancer, research, contents, history, characteristics, growth, conditions, references, external, linkshistory, characteristics, editformally, known, abbreviation, uppsala, malignant, g. In cell biology U 87 MG is a human glioblastoma cell line that is commonly used in brain cancer research 1 Contents 1 History and Characteristics 2 Growth conditions 3 References 4 External linksHistory and Characteristics editFormally known as U 87 MG abbreviation for Uppsala 87 Malignant Glioma the U87 cell line has an epithelial morphology and was obtained from a 44 year old female patient in 1966 at Uppsala University The cell line was thought to be deposited at the Memorial Sloan Kettering Cancer Center in 1973 after which the ATCC obtained it in 1982 2 However in 2016 the commonly used version of U87MG from the ATCC was found to be non identical to its patient of origin 3 Analysis of DNA profile and mtDNA position suggest that the Uppsala U 87 MG line is authentic to the original tumor tissue while the ATCC U 87 MG line is not The ATCC line is likely a bona fide human glioblastoma cell line of unknown origin 3 The entire sequence of the genome of U 87 MG has been published in PLoS Genetics 1 U 87 MG can be obtained from the American Type Culture Collection ATCC where it is known by the accession number HTB 14 and it is reported that the cell line comes from a male patient of unknown age 4 Growth conditions editU87 growth media is generally made with Eagle s minimum essential medium 10 FBS 100 U mL penicillin 100 µg mL streptomycin 4 It is propagated at 37 C in a 5 carbon dioxide atmosphere References edit a b Clark MJ Homer N O Connor BD et al January 2010 U87MG decoded the genomic sequence of a cytogenetically aberrant human cancer cell line PLOS Genet 6 1 e1000832 doi 10 1371 journal pgen 1000832 PMC 2813426 PMID 20126413 Dolgin Ellie 31 August 2016 Venerable brain cancer cell line faces identity crisis Nature 537 7619 149 150 Bibcode 2016Natur 537 149D doi 10 1038 nature 2016 20515 PMID 27604929 S2CID 4460070 Retrieved 3 May 2018 a b Allen M Bjerke M Edlund H Nelander S Westermark B Aug 2016 Origin of the U87MG glioma cell line Good news and bad news Sci Transl Med 8 354 354re3 doi 10 1126 scitranslmed aaf6853 PMID 27582061 S2CID 4577520 a b HTB 14 ATCC External links editATCC record for HTB 14 Cellosaurus entry for U87 nbsp This cell biology article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title U 87 MG amp oldid 1185203638, wikipedia, wiki, book, books, library,
