Truenat is a chip-based, point-of-care, rapid molecular test for diagnosis of infectious diseases. The technology is based on the Taqman RTPCR (Real Time Reverse Transcription Polymerase Chain Reaction) chemistry which can be performed on the portable, battery operated Truelab Real Time micro PCR platform. Truenat is developed and manufactured by Goa-based Molbio Diagnostics Private Limited.[1][2][3][4]
Based on findings from a multi-centre diagnostic accuracy assessment conducted by The Foundation for Innovative New Diagnostics (FIND), the World Health Organization (WHO) announced endorsement of Molbio's molecular assays Truenat MTB, Truenat MTB Plus and Truenat MTB RIFDx as initial diagnostic tests of pulmonary Tuberculosis and Rifampicin Resistance through a rapid communication in January,2020. The Truenat test was incorporated in India's National Tuberculosis Elimination Programme after recommendations from the Indian Council of Medical Research (ICMR).[5][6][7][8]
The Government of Andhra Pradesh was one of the first adopter of Truenat under its Revised National Tuberculosis Control Programme (RNTCP) for TB diagnosis. In October 2018, the state rolled out Truenat in Designated Microscopy Centres (DMC) across 13 districts. Truenat was found to improved TB case notification rates by 30% in Andhra Pradesh [9]
Truenat for COVID-19edit
In April, 2020 the point-of-care Truenat test for diagnosis of COVID-19 was launched after validation at the State Level Virus Research and Diagnostic Laboratory, Bangalore Medical College and Research Institute and subsequent approval from the Indian Council of Medical Research (ICMR).[10] The test processes oropharyngeal and nasopharyngeal swab specimen and provides results within one hour from sample collection.[11]
Samples are collected in a viral lysis buffer that require very minimal biosafety and biosecurity.[12]The Lancet remarked, "This innovative technology-driven COVID-19 testing platform has been a game changer for testing in underserved areas and quick testing in emergency departments of health-care facilities in India."[12]
Technologyedit
The Truenat test is run on the battery-powered Truelab system. This system consists of a sample preparation device ( this device is used for RNA/DNA extraction and then the purification from the sample) and along with that - the PCR analyzer device. This device is available in 1-, 2-, or 4-module configurations. The lattermost configuration is capable of testing four samples at the same time.[13]
The devices function in a wide range of environmental conditions with minimal user input, making them suitable for use in primary healthcare settings with minimal infrastructural requirements, providing an automated reporting system and having real time data transfer capability for centralized monitoring and analytics for disease surveillance.[14]
^Basu, Mohana (2020-06-27). "RT-PCR, antigen, antibody, TrueNAT — all you need to know about the different Covid tests". ThePrint. Retrieved 2020-12-02.
^"New coronavirus testing kit gives results in just an hour". Deccan Herald. 2020-04-06. Retrieved 2020-12-02.
^"COVID-19 testing: How antibody, antigen, RT-PCR, TrueNat tests differ, their strengths and limitations - Health News, Firstpost". Firstpost. 2020-07-09. Retrieved 2020-12-02.
^"World Health Organization endorses Truenat tests for initial diagnosis of tuberculosis and detection of rifampicin resistance". FIND. Retrieved 2020-12-02.
^Perappadan, Bindu Shajan (2020-07-03). "WHO nod for Truenat rapid molecular assays for TB". The Hindu. ISSN 0971-751X. Retrieved 2020-12-02.
^Ravindranath, Prasad (2020-01-17). "India's TB control programme renamed to reflect its intent on ending TB". Science Chronicle. Retrieved 2020-12-02.
^Jeyashree, K.; Shanmugasundaram, D.; Rade, K.; Gangakhedkar, R. R.; Murhekar, M. V. (2020-09-21). "Impact and operational feasibility of TrueNat™ MTB/Rif under India's RNTCP". Public Health Action. 10 (3): 87–91. doi:10.5588/pha.20.0004. ISSN 2220-8372. PMC7577004. PMID 33134121.
^"ICMR validates indigenous assays for COVID-19 diagnosis".
^"Covid-19 testing: What are the tests and testing procedures being carried out in India?". The Indian Express. 2020-07-28. Retrieved 2020-12-04.
^ abGupta, Nivedita; Rana, Salaj; Singh, Harpreet (2020-11-01). "Innovative point-of-care molecular diagnostic test for COVID-19 in India". The Lancet Microbe. 1 (7): e277. doi:10.1016/S2666-5247(20)30164-6. ISSN 2666-5247. PMC7833111. PMID 33521725.
^Lee, David J.; Kumarasamy, Nagalingeswaran; Resch, Stephen C.; Sivaramakrishnan, Gomathi N.; Mayer, Kenneth H.; Tripathy, Srikanth; Paltiel, A. David; Freedberg, Kenneth A.; Reddy, Krishna P. (2019-07-02). "Rapid, point-of-care diagnosis of tuberculosis with novel Truenat assay: Cost-effectiveness analysis for India's public sector". PLOS ONE. 14 (7): e0218890. Bibcode:2019PLoSO..1418890L. doi:10.1371/journal.pone.0218890. ISSN 1932-6203. PMC6605662. PMID 31265470.
truenat, this, article, multiple, issues, please, help, improve, discuss, these, issues, talk, page, learn, when, remove, these, template, messages, this, article, reads, like, press, release, news, article, largely, based, routine, coverage, please, help, imp. This article has multiple issues Please help improve it or discuss these issues on the talk page Learn how and when to remove these template messages This article reads like a press release or a news article and may be largely based on routine coverage Please help improve this article and add independent sources January 2021 A major contributor to this article appears to have a close connection with its subject It may require cleanup to comply with Wikipedia s content policies particularly neutral point of view Please discuss further on the talk page January 2021 Learn how and when to remove this template message Learn how and when to remove this template message Truenat is a chip based point of care rapid molecular test for diagnosis of infectious diseases The technology is based on the Taqman RTPCR Real Time Reverse Transcription Polymerase Chain Reaction chemistry which can be performed on the portable battery operated Truelab Real Time micro PCR platform Truenat is developed and manufactured by Goa based Molbio Diagnostics Private Limited 1 2 3 4 Contents 1 Truenat for TB 2 Truenat for COVID 19 3 Technology 4 ReferencesTruenat for TB editBased on findings from a multi centre diagnostic accuracy assessment conducted by The Foundation for Innovative New Diagnostics FIND the World Health Organization WHO announced endorsement of Molbio s molecular assays Truenat MTB Truenat MTB Plus and Truenat MTB RIFDx as initial diagnostic tests of pulmonary Tuberculosis and Rifampicin Resistance through a rapid communication in January 2020 The Truenat test was incorporated in India s National Tuberculosis Elimination Programme after recommendations from the Indian Council of Medical Research ICMR 5 6 7 8 The Government of Andhra Pradesh was one of the first adopter of Truenat under its Revised National Tuberculosis Control Programme RNTCP for TB diagnosis In October 2018 the state rolled out Truenat in Designated Microscopy Centres DMC across 13 districts Truenat was found to improved TB case notification rates by 30 in Andhra Pradesh 9 Truenat for COVID 19 editIn April 2020 the point of care Truenat test for diagnosis of COVID 19 was launched after validation at the State Level Virus Research and Diagnostic Laboratory Bangalore Medical College and Research Institute and subsequent approval from the Indian Council of Medical Research ICMR 10 The test processes oropharyngeal and nasopharyngeal swab specimen and provides results within one hour from sample collection 11 Samples are collected in a viral lysis buffer that require very minimal biosafety and biosecurity 12 The Lancet remarked This innovative technology driven COVID 19 testing platform has been a game changer for testing in underserved areas and quick testing in emergency departments of health care facilities in India 12 Technology editThe Truenat test is run on the battery powered Truelab system This system consists of a sample preparation device this device is used for RNA DNA extraction and then the purification from the sample and along with that the PCR analyzer device This device is available in 1 2 or 4 module configurations The lattermost configuration is capable of testing four samples at the same time 13 The devices function in a wide range of environmental conditions with minimal user input making them suitable for use in primary healthcare settings with minimal infrastructural requirements providing an automated reporting system and having real time data transfer capability for centralized monitoring and analytics for disease surveillance 14 References edit Shankar Sathyanarayanmuthur Basawarajappa Shantalagowdara Rangaiah Ambica Padukone Shashiraja Yadav Pragyad Gupta Nivedita 2021 Performance evaluation of Truenat Beta CoV amp Truenat SARS CoV 2 point of care assays for coronavirus disease 2019 Indian J Med Res 153 1 amp 2 144 150 doi 10 4103 ijmr IJMR 2363 20 PMC 8184085 PMID 33818471 Basu Mohana 2020 06 27 RT PCR antigen antibody TrueNAT all you need to know about the different Covid tests ThePrint Retrieved 2020 12 02 New coronavirus testing kit gives results in just an hour Deccan Herald 2020 04 06 Retrieved 2020 12 02 COVID 19 testing How antibody antigen RT PCR TrueNat tests differ their strengths and limitations Health News Firstpost Firstpost 2020 07 09 Retrieved 2020 12 02 WHO endorses Truenat molecular diagnostic tests for TB News Medical net 2020 07 03 Retrieved 2020 12 02 World Health Organization endorses Truenat tests for initial diagnosis of tuberculosis and detection of rifampicin resistance FIND Retrieved 2020 12 02 Perappadan Bindu Shajan 2020 07 03 WHO nod for Truenat rapid molecular assays for TB The Hindu ISSN 0971 751X Retrieved 2020 12 02 Ravindranath Prasad 2020 01 17 India s TB control programme renamed to reflect its intent on ending TB Science Chronicle Retrieved 2020 12 02 Jeyashree K Shanmugasundaram D Rade K Gangakhedkar R R Murhekar M V 2020 09 21 Impact and operational feasibility of TrueNat MTB Rif under India s RNTCP Public Health Action 10 3 87 91 doi 10 5588 pha 20 0004 ISSN 2220 8372 PMC 7577004 PMID 33134121 ICMR validates indigenous assays for COVID 19 diagnosis Covid 19 testing What are the tests and testing procedures being carried out in India The Indian Express 2020 07 28 Retrieved 2020 12 04 a b Gupta Nivedita Rana Salaj Singh Harpreet 2020 11 01 Innovative point of care molecular diagnostic test for COVID 19 in India The Lancet Microbe 1 7 e277 doi 10 1016 S2666 5247 20 30164 6 ISSN 2666 5247 PMC 7833111 PMID 33521725 Lee David J Kumarasamy Nagalingeswaran Resch Stephen C Sivaramakrishnan Gomathi N Mayer Kenneth H Tripathy Srikanth Paltiel A David Freedberg Kenneth A Reddy Krishna P 2019 07 02 Rapid point of care diagnosis of tuberculosis with novel Truenat assay Cost effectiveness analysis for India s public sector PLOS ONE 14 7 e0218890 Bibcode 2019PLoSO 1418890L doi 10 1371 journal pone 0218890 ISSN 1932 6203 PMC 6605662 PMID 31265470 WHO endorses Truenat molecular diagnostic tests for TB News Medical net 2020 07 03 Retrieved 2020 12 04 Retrieved from https en wikipedia org w index php title Truenat amp oldid 1187579346, wikipedia, wiki, book, books, library,
