TRIzol is a widely used[1] chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987.[2]
The correct name of the method is guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to >50% bigger RNA yield.[5][6] An alternative method for RNA extraction is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.
DNA and RNA from TRIzol and TRI reagent can also be extracted using the Direct-zol Miniprep kit by Zymo Research.[7] This method eliminates the use of Chloroform and 1-bromo-3-chloropropane completely, bypassing phase-separation and precipitation steps.[8]
TRIzol is light-sensitive and is often stored in a dark-colored, glass container covered in foil. It is stored at room temperature.
When used, it resembles cough syrup, bright pink. The smell of the phenol is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.
Hazardsedit
Vigilant caution should be taken while using TRIzol (due to the phenol and chloroform).
TRIzol is labeled as acute oral, dermal, and inhalation toxicity besides skin corrosion/irritation in the manufacturer MDS.[9]
Exposure to TRIzol can be a serious health hazard. Exposure can lead to serious chemical burns, permanent scarring and kidney failure.
Experiments should be performed under a chemical hood, with lab coat, nitrile gloves and a plastic apron. [10][11]
TRIzol waste should never be mixed with bleach or acids: the guanidinium thiocyanate in TRIzol reacts to form highly toxic gases.
Referencesedit
^"Total RNA isolation: Trizol vs RNeasy". www.bioastrum.com. Retrieved 2021-08-18.
^Chomczynski, P; Sacchi, N (April 1987). "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction". Anal. Biochem. 162 (1): 156–9. doi:10.1016/0003-2697(87)90021-2. PMID 2440339.
^(PDF). Cell Analysis Facility. Archived from the original (PDF) on 2021-08-18. Retrieved 2021-08-18.
^"Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque. - Biospecimen Research Database". brd.nci.nih.gov. Retrieved 2021-08-18.
^Material Safety data sheet from Life technologies. 2008-07-21 at the Wayback Machine
^Material Safety data sheet from MRC 2008-12-28 at the Wayback Machine
External linksedit
RNA extraction using trizol protocol on OpenWetWare
March 14, 2024
trizol, trizol, widely, used, chemical, solution, used, extraction, proteins, from, cells, solution, initially, used, published, piotr, chomczyński, nicoletta, sacchi, 1987, trizol, reagent, contains, guanidinium, thiocyanate, phenol, trizol, brand, name, guan. TRIzol is a widely used 1 chemical solution used in the extraction of DNA RNA and proteins from cells The solution was initially used and published by Piotr Chomczynski and Nicoletta Sacchi in 1987 2 TRIzol reagent contains guanidinium thiocyanate and phenol TRIzol is the brand name of guanidinium thiocyanate from the Ambion part of Life Technologies 3 and Tri Reagent is the brand name from MRC 4 which was founded by Chomczynski Contents 1 Uses in extraction 2 Hazards 3 References 4 External linksUses in extraction editThe correct name of the method is guanidinium thiocyanate phenol chloroform extraction The use of TRIzol can result in DNA yields comparable to other extraction methods and it leads to gt 50 bigger RNA yield 5 6 An alternative method for RNA extraction is phenol extraction and TCA acetone precipitation Chloroform should be exchanged with 1 bromo 3 chloropropane when using the new generation TRI Reagent DNA and RNA from TRIzol and TRI reagent can also be extracted using the Direct zol Miniprep kit by Zymo Research 7 This method eliminates the use of Chloroform and 1 bromo 3 chloropropane completely bypassing phase separation and precipitation steps 8 TRIzol is light sensitive and is often stored in a dark colored glass container covered in foil It is stored at room temperature When used it resembles cough syrup bright pink The smell of the phenol is extremely strong TRIzol works by maintaining RNA integrity during tissue homogenization while at the same time disrupting and breaking down cells and cell components Hazards editVigilant caution should be taken while using TRIzol due to the phenol and chloroform TRIzol is labeled as acute oral dermal and inhalation toxicity besides skin corrosion irritation in the manufacturer MDS 9 Exposure to TRIzol can be a serious health hazard Exposure can lead to serious chemical burns permanent scarring and kidney failure Experiments should be performed under a chemical hood with lab coat nitrile gloves and a plastic apron 10 11 TRIzol waste should never be mixed with bleach or acids the guanidinium thiocyanate in TRIzol reacts to form highly toxic gases References edit Total RNA isolation Trizol vs RNeasy www bioastrum com Retrieved 2021 08 18 Chomczynski P Sacchi N April 1987 Single step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction Anal Biochem 162 1 156 9 doi 10 1016 0003 2697 87 90021 2 PMID 2440339 Life Technologies Molecular Research Center Inc RNA isolation PDF Cell Analysis Facility Archived from the original PDF on 2021 08 18 Retrieved 2021 08 18 Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque Biospecimen Research Database brd nci nih gov Retrieved 2021 08 18 Direct zol RNA Microprep Kits Nucleic acid purification TRIZOl MDS PDF Material Safety data sheet from Life technologies Archived 2008 07 21 at the Wayback Machine Material Safety data sheet from MRC Archived 2008 12 28 at the Wayback MachineExternal links editRNA extraction using trizol protocol on OpenWetWare Retrieved from https en wikipedia org w index php title Trizol amp oldid 1197934246, wikipedia, wiki, book, books, library,
