Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are:[1]
Increased CO2 production on phagocytosis and differentiation into macrophage-like cells
Polarization into the M1 phenotype by incubation with IFN-γ and LPS, or to the M2 phenotype by incubation with interleukin 4 and interleukin 13[2]
Differentiation into immature dendritic cells, using recombinant human interleukin 4 (rhIL-4) and recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF), and mature dendritic cells using rhIL-4, rhGM-CSF, recombinant human tumour necrotic factor α (rhTNF-α) and Ionomycin.[3]
The HLA type for THP-1 is HLA-A*02:01; A*24:02; B*15:11; B*35:01; C*03:03; DRB1*01:01; DRB1*15:01; DQB1*05:01; DQB1*06:02; DPB1*02:01; DPB1*04:02 (in the German Collection of Microorganisms and Cell Cultures (DSMZ) cell bank). This HLA type can change depending on the reference biorepository, due to loss of heterozygosity in multiple chromosomal regions, as THP-1 from the American Type Culture Collection (ATCC) do not express the HLA-A*24:02 and B*35:01 alleles.[4]
Growth Informationedit
THP-1 can provide continuous culture when grown in suspension; RPMI 1640 + 10% FBS + 2mM L-Glutamine. The average doubling time is 19 to 50 hours. 1 mM sodium pyruvate, penicillin (100 units/ml) and streptomycin (100 μg/ml) are also commonly added to inhibit bacterial contamination. Cultures should be maintained at cell densities in the range 2-9x105 cells/ml at 37 °C, 5% CO2. Cells are non-adherent.[5]
Hazardsedit
THP-1 cells are of human origin, and no evidence has been found for the presence of infectious viruses or toxic products. The ATCC Biosafety recommendation is level 1.[5]
Research applicationsedit
THP-1 cells are used as a models to study the monocyte-macrophage differentiation process,[6] and as a model to examine some macrophage-related physiological processes, for example the macrophage cholesterol efflux.[7]
Referencesedit
^ abTsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K (August 1980). "Establishment and characterization of a human acute monocytic leukemia cell line (THP-1)". International Journal of Cancer. 26 (2): 171–6. doi:10.1002/ijc.2910260208. PMID 6970727. S2CID 43603660.
^Genin M, Clement F, Fattaccioli A, Raes M, Michiels C (August 2015). "M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide". BMC Cancer. 15: 577. doi:10.1186/s12885-015-1546-9. PMC4545815. PMID 26253167.
^Berges C, Naujokat C, Tinapp S, Wieczorek H, Höh A, Sadeghi M, Opelz G, Daniel V (August 2005). "A cell line model for the differentiation of human dendritic cells". Biochemical and Biophysical Research Communications. 333 (3): 896–907. doi:10.1016/j.bbrc.2005.05.171. PMID 15963458.
^Noronha N, Ehx G, Meunier MC, Laverdure JP, Thériault C, Perreault C (March 2020). "Major multi-level molecular divergence between THP-1 cells from different biorepositories". International Journal of Cancer. xxx (x): 2000–2006. doi:10.1002/ijc.32967. PMID 32163592. S2CID 212692034.
^Auwerx J (January 1991). "The human leukemia cell line, THP-1: a multifacetted model for the study of monocyte-macrophage differentiation". Experientia. 47 (1): 22–31. doi:10.1007/BF02041244. PMID 1999239. S2CID 24727878.
^Wang D, Hiebl V, Ladurner A, Latkolik SL, Bucar F, Heiß EH, Dirsch VM, Atanasov AG (May 2018). "6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-derived Macrophages". Molecular Nutrition & Food Research. 62 (14): e1800011. doi:10.1002/mnfr.201800011. PMC6099374. PMID 29802792.
External linksedit
Cellosaurus entry for THP-1
April 12, 2024
cell, line, this, article, includes, list, general, references, lacks, sufficient, corresponding, inline, citations, please, help, improve, this, article, introducing, more, precise, citations, september, 2017, learn, when, remove, this, template, message, hum. This article includes a list of general references but it lacks sufficient corresponding inline citations Please help to improve this article by introducing more precise citations September 2017 Learn how and when to remove this template message THP 1 is a human monocytic cell line derived from an acute monocytic leukemia patient It is used to test leukemia cell lines in immunocytochemical analysis of protein protein interactions and immunohistochemistry 1 Contents 1 Characteristics 1 1 Growth Information 1 2 Hazards 1 3 Research applications 2 References 3 External linksCharacteristics editAlthough THP 1 cells are of the same lineage mutations can cause differences as the progeny proliferates In general THP 1 cells exhibit a large round single cell morphology The cells were derived from the peripheral blood of a 1 year old human male with acute monocytic leukemia Some of their characteristics are 1 Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglobulins Production of IL 1 Positive detection of alpha naphthyl butyrate esterase and lysozymes Phagocytic physiology both for latex beads and sensitized erythrocytes Restoration of the response of purified T lymphocytes to Con A Increased CO2 production on phagocytosis and differentiation into macrophage like cells Polarization into the M1 phenotype by incubation with IFN g and LPS or to the M2 phenotype by incubation with interleukin 4 and interleukin 13 2 Differentiation into immature dendritic cells using recombinant human interleukin 4 rhIL 4 and recombinant human granulocyte macrophage colony stimulating factor rhGM CSF and mature dendritic cells using rhIL 4 rhGM CSF recombinant human tumour necrotic factor a rhTNF a and Ionomycin 3 The HLA type for THP 1 is HLA A 02 01 A 24 02 B 15 11 B 35 01 C 03 03 DRB1 01 01 DRB1 15 01 DQB1 05 01 DQB1 06 02 DPB1 02 01 DPB1 04 02 in the German Collection of Microorganisms and Cell Cultures DSMZ cell bank This HLA type can change depending on the reference biorepository due to loss of heterozygosity in multiple chromosomal regions as THP 1 from the American Type Culture Collection ATCC do not express the HLA A 24 02 and B 35 01 alleles 4 Growth Information edit THP 1 can provide continuous culture when grown in suspension RPMI 1640 10 FBS 2mM L Glutamine The average doubling time is 19 to 50 hours 1 mM sodium pyruvate penicillin 100 units ml and streptomycin 100 mg ml are also commonly added to inhibit bacterial contamination Cultures should be maintained at cell densities in the range 2 9x105 cells ml at 37 C 5 CO2 Cells are non adherent 5 Hazards edit THP 1 cells are of human origin and no evidence has been found for the presence of infectious viruses or toxic products The ATCC Biosafety recommendation is level 1 5 Research applications edit THP 1 cells are used as a models to study the monocyte macrophage differentiation process 6 and as a model to examine some macrophage related physiological processes for example the macrophage cholesterol efflux 7 References edit a b Tsuchiya S Yamabe M Yamaguchi Y Kobayashi Y Konno T Tada K August 1980 Establishment and characterization of a human acute monocytic leukemia cell line THP 1 International Journal of Cancer 26 2 171 6 doi 10 1002 ijc 2910260208 PMID 6970727 S2CID 43603660 Genin M Clement F Fattaccioli A Raes M Michiels C August 2015 M1 and M2 macrophages derived from THP 1 cells differentially modulate the response of cancer cells to etoposide BMC Cancer 15 577 doi 10 1186 s12885 015 1546 9 PMC 4545815 PMID 26253167 Berges C Naujokat C Tinapp S Wieczorek H Hoh A Sadeghi M Opelz G Daniel V August 2005 A cell line model for the differentiation of human dendritic cells Biochemical and Biophysical Research Communications 333 3 896 907 doi 10 1016 j bbrc 2005 05 171 PMID 15963458 Noronha N Ehx G Meunier MC Laverdure JP Theriault C Perreault C March 2020 Major multi level molecular divergence between THP 1 cells from different biorepositories International Journal of Cancer xxx x 2000 2006 doi 10 1002 ijc 32967 PMID 32163592 S2CID 212692034 a b THP 1 ATCC Retrieved 19 June 2018 Auwerx J January 1991 The human leukemia cell line THP 1 a multifacetted model for the study of monocyte macrophage differentiation Experientia 47 1 22 31 doi 10 1007 BF02041244 PMID 1999239 S2CID 24727878 Wang D Hiebl V Ladurner A Latkolik SL Bucar F Heiss EH Dirsch VM Atanasov AG May 2018 6 Dihydroparadol a Ginger Constituent Enhances Cholesterol Efflux from THP 1 derived Macrophages Molecular Nutrition amp Food Research 62 14 e1800011 doi 10 1002 mnfr 201800011 PMC 6099374 PMID 29802792 External links editCellosaurus entry for THP 1 Retrieved from https en wikipedia org w index php title THP 1 cell line amp oldid 1187414303, wikipedia, wiki, book, books, library,
