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Soluble adenylyl cyclase

April 14, 2024

See also: ADCY10

Soluble adenylyl cyclase (sAC) is a regulatory cytosolic enzyme present in almost every cell. sAC is a source of cyclic adenosine 3’,5’ monophosphate (cAMP) – a second messenger that mediates cell growth and differentiation in organisms from bacteria to higher eukaryotes. sAC differentiates from the transmembrane adenylyl cyclase (tmACs) – an important source of cAMP; in that sAC is regulated by bicarbonate anions and it is dispersed throughout the cell cytoplasm. sAC has been found to have various functions in physiological systems different from that of the tmACs.[1]

Genomic context and summary edit

 
Gene Structure: Chromosome: 1; NC_000001.10 (167778625..167883453, complement)

sAC is encoded in a single Homo sapiens gene identified as ADCY10 or Adenylate cyclase 10 (soluble). This gene packed down 33 exons that comprise greater than 100kb; though, it seems to utilize multiple promoters,[2][3] and its mRNA undergoes extensive alternative splicing.[2][3][4][5]

Structure edit

The functional mammalian sAC consist of two heterologous catalytic domains (C1 and C2), forming the 50 kDa amino terminus of the protein.[6][7] The additional ~140 kDa C terminus of the enzyme includes an autoinhibitory region,[8] canonical P-loop, potential heme-binding domain,[9] and leucine zipper-like sequence,[10] which are a form of putative regulatory domains.

A truncated form of the enzyme only includes the C1 and C2 domains and it is refers to as the minimal functional sAC variant.[5][10] This sAC-truncated form has cAMP-forming activity much higher than its full-length type. These sAC variants are stimulated by HCO3- and respond to all known selective sAC inhibitors.[6] Crystal structures of this sAC variant comprising only the catalytic core, in apo form and in as complex with various substrate analogs, products, and regulators, reveal a generic Class III AC architecture with sAC-specific features.[11] The structurally related domains C1 and C2 form the typical pseudo-heterodimer, with one active site.[6] The pseudo-symmetric site accommodates the sAC-specific activator HCO3−, which activates by triggering a rearrangement of Arg176, a residue connecting both sites. The anionic sAC inhibitor 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) acts as a blocker for the entrance to active site and bicarbonate binding pocket.[11]

Activation by bicarbonate (HCO−3) and calcium (Ca2+) edit

The binding and cyclizing of adenosine 5’ triphosphate (ATP) to the catalytic active site of the enzyme is coordinated by two metal cations. The catalytic activity of sAC is increase by the presence of manganese [Mn2+]. sAC magnesium [Mg2+] activity is regulated by calcium [Ca2+] which increases the affinity for ATP of mammalian sAC. In addition, bicarbonate [HCO−3] releases ATP-Mg2+ substrate inhibition and increases Vmax of the enzyme.[12]

The open conformation state of sAC is reached when ATP, with Ca2+ bound to its γ-phosphate binds with specific residues in the catalytic center of the enzyme. When the second metal – a Mg2+ ion – binds to the α-phosphate of ATP leads to a conformational change of the enzyme: the close state. The change in conformation from open to close state induces esterification of the α-phosphate with the ribose in adenosine and the release of the β- and γ-phosphates, this leads to cyclizing.[7] Hydrogencarbonate stimulates the enzyme’s Vmax by promoting the allosteric change that leads to active site closure, recruitment of the catalytic Mg2+ ion, and readjustment of the phosphates in the bound ATP.[13] The activator bicarbonate binds to a site pseudo-symmetric to the active site and triggers conformational changes by recruiting Arg176 from the active site (see above - "structure").[11] Calcium increases substrate affinity by replacing the magnesium in the ion B site, which provides an anchoring point for the beta- and gamma-phosphates of the ATP substrate.[11][13]

Sources of bicarbonate (HCO−3)and calcium (Ca2+) edit

  • bicarbonate derived from carbonic anhydrase (CA)-dependent hydration.
  • CO2 metabolism
  • Enters through membrane-transporting proteins or cystic fibrosis transmembrane conductance regulators.
  • Calcium enters by voltage-dependent Ca2+ channels or by release from the endoplasmic reticulum.
  • Hydrogencarbonate and calcium activates sAC in the nucleus.
  • sAC inside mitochondria is activated by metabolically generated CO2 through carbonic anhydrase.

Physiological effects edit

Brain and nervous system edit

Astrocytes express several sAC splice variants,[14] which are involved in metabolic coupling between neurons and astrocytes. Increase of potassium [K+] in the extracellular space caused by neuronal activity depolarizes the cell membrane of nearby astrocytes and facilitates the entry of hydrogencarbonate through Na+/HCO−3- cotransporters.[7] The increase in cytosolic hydrogencarbonate activates sAC; the result of this activation is the release of lactate for use as energy source by the neurons.

Bone edit

Numerous sAC splice variants are present in osteoclast and osteoblasts,[3] and mutation in the human sAC gene is associated with low spinal density.[15] Calcification by osteoblasts is intrinsically related with bicarbonate and calcium. Bone density experiments in mouse calvaria [16] cultured indicates that HCO−3-sensing sAC is a physiological appropriate regulator of bone formation and/or reabsorption.

Sperm edit

sAC activation by bicarbonate is necessary for motility and other aspects of capacitation in the spermatozoa of mammals.[17][18] In human males, mutations in the ADCY10 gene that lead to the inactivation of sAC have been linked to cases of sterility.[19] Due to this essential role in male fertility, sAC has been explored as a potential target for non-hormonal male contraception. [20]

References edit

  1. ^ Rossetti T, Jackvony S, Buck J, Levin LR (April 2021). "Bicarbonate, carbon dioxide and pH sensing via mammalian bicarbonate-regulated soluble adenylyl cyclase". Interface Focus. 11 (2): 20200034. doi:10.1098/rsfs.2020.0034. PMC 7898154. PMID 33633833.
  2. ^ a b Farrell J, Ramos L, Tresguerres M, Kamenetsky M, Levin LR, Buck J (September 2008). "Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice". PLOS ONE. 3 (9): e3251. Bibcode:2008PLoSO...3.3251F. doi:10.1371/journal.pone.0003251. PMC 2532759. PMID 18806876.
  3. ^ a b c Geng W, Wang Z, Zhang J, Reed BY, Pak CY, Moe OW (June 2005). "Cloning and characterization of the human soluble adenylyl cyclase". American Journal of Physiology. Cell Physiology. 288 (6): C1305–C1316. doi:10.1152/ajpcell.00584.2004. PMID 15659711. S2CID 1638649.
  4. ^ Schmid A, Sutto Z, Nlend MC, Horvath G, Schmid N, Buck J, et al. (July 2007). "Soluble adenylyl cyclase is localized to cilia and contributes to ciliary beat frequency regulation via production of cAMP". The Journal of General Physiology. 130 (1): 99–109. doi:10.1085/jgp.200709784. PMC 2154360. PMID 17591988.
  5. ^ a b Jaiswal BS, Conti M (August 2001). "Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase". The Journal of Biological Chemistry. 276 (34): 31698–31708. doi:10.1074/jbc.m011698200. PMID 11423534.
  6. ^ a b c Steegborn C (December 2014). "Structure, mechanism, and regulation of soluble adenylyl cyclases - similarities and differences to transmembrane adenylyl cyclases". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1842 (12 Pt B): 2535–2547. doi:10.1016/j.bbadis.2014.08.012. PMID 25193033.
  7. ^ a b c Tresguerres M, Levin LR, Buck J (June 2011). "Intracellular cAMP signaling by soluble adenylyl cyclase". Kidney International. 79 (12): 1277–1288. doi:10.1038/ki.2011.95. PMC 3105178. PMID 21490586.
  8. ^ Chaloupka JA, Bullock SA, Iourgenko V, Levin LR, Buck J (March 2006). "Autoinhibitory regulation of soluble adenylyl cyclase". Molecular Reproduction and Development. 73 (3): 361–368. doi:10.1002/mrd.20409. PMC 3644951. PMID 16250004.
  9. ^ Middelhaufe S, Leipelt M, Levin LR, Buck J, Steegborn C (October 2012). "Identification of a haem domain in human soluble adenylate cyclase". Bioscience Reports. 32 (5): 491–499. doi:10.1042/BSR20120051. PMC 3475452. PMID 22775536.
  10. ^ a b Buck J, Sinclair ML, Schapal L, Cann MJ, Levin LR (January 1999). "Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals". Proceedings of the National Academy of Sciences of the United States of America. 96 (1): 79–84. Bibcode:1999PNAS...96...79B. doi:10.1073/pnas.96.1.79. PMC 15096. PMID 9874775.
  11. ^ a b c d Kleinboelting S, Diaz A, Moniot S, van den Heuvel J, Weyand M, Levin LR, et al. (March 2014). "Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate". Proceedings of the National Academy of Sciences of the United States of America. 111 (10): 3727–3732. Bibcode:2014PNAS..111.3727K. doi:10.1073/pnas.1322778111. PMC 3956179. PMID 24567411.
  12. ^ Litvin TN, Kamenetsky M, Zarifyan A, Buck J, Levin LR (May 2003). "Kinetic properties of "soluble" adenylyl cyclase. Synergism between calcium and bicarbonate". The Journal of Biological Chemistry. 278 (18): 15922–15926. doi:10.1074/jbc.m212475200. PMID 12609998.
  13. ^ a b Steegborn C, Litvin TN, Levin LR, Buck J, Wu H (January 2005). "Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment". Nature Structural & Molecular Biology. 12 (1): 32–37. doi:10.1038/nsmb880. PMC 3644947. PMID 15619637.
  14. ^ Choi HB, Gordon GR, Zhou N, Tai C, Rungta RL, Martinez J, et al. (September 2012). "Metabolic communication between astrocytes and neurons via bicarbonate-responsive soluble adenylyl cyclase". Neuron. 75 (6): 1094–1104. doi:10.1016/j.neuron.2012.08.032. PMC 3630998. PMID 22998876.
  15. ^ Reed BY, Gitomer WL, Heller HJ, Hsu MC, Lemke M, Padalino P, Pak CY (April 2002). "Identification and characterization of a gene with base substitutions associated with the absorptive hypercalciuria phenotype and low spinal bone density". The Journal of Clinical Endocrinology and Metabolism. 87 (4): 1476–1485. doi:10.1210/jcem.87.4.8300. PMID 11932268.
  16. ^ Geng W, Hill K, Zerwekh JE, Kohler T, Müller R, Moe OW (August 2009). "Inhibition of osteoclast formation and function by bicarbonate: role of soluble adenylyl cyclase". Journal of Cellular Physiology. 220 (2): 332–340. doi:10.1002/jcp.21767. PMC 3044925. PMID 19360717.
  17. ^ Xie F, Garcia MA, Carlson AE, Schuh SM, Babcock DF, Jaiswal BS, et al. (August 2006). "Soluble adenylyl cyclase (sAC) is indispensable for sperm function and fertilization". Developmental Biology. 296 (2): 353–362. doi:10.1016/j.ydbio.2006.05.038. PMID 16842770.
  18. ^ Wennemuth G, Carlson AE, Harper AJ, Babcock DF (April 2003). "Bicarbonate actions on flagellar and Ca2+ -channel responses: initial events in sperm activation". Development. 130 (7): 1317–1326. doi:10.1242/dev.00353. PMID 12588848.
  19. ^ Akbari, Arvand; Pipitone, Giovanni Battista; Anvar, Zahra; Jaafarinia, Mojtaba; Ferrari, Maurizio; Carrera, Paola; Totonchi, Mehdi (4 June 2019). "ADCY10 frameshift variant leading to severe recessive asthenozoospermia and segregating with absorptive hypercalciuria". Human Reproduction. 34 (6): 1155–1164. doi:10.1093/humrep/dez048. PMID 31119281.
  20. ^ Balbach, Melanie; Rossetti, Thomas; Ferreira, Jacob; Ghanem, Lubna; Ritagliati, Carla; Myers, Robert W.; Huggins, David J.; Steegborn, Clemens; Miranda, Ileana C.; Meinke, Peter T.; Buck, Jochen; Levin, Lonny R. (14 February 2023). "On-demand male contraception via acute inhibition of soluble adenylyl cyclase". Nature Communications. 14 (1): 637. Bibcode:2023NatCo..14..637B. doi:10.1038/s41467-023-36119-6. PMC 9929232. PMID 36788210.

Further reading edit

  • Tresguerres M, Levin LR, Buck J (June 2011). "Intracellular cAMP signaling by soluble adenylyl cyclase". Kidney International. 79 (12): 1277–1288. doi:10.1038/ki.2011.95. PMC 3105178. PMID 21490586.
  • Chen Y, Cann MJ, Litvin TN, Iourgenko V, Sinclair ML, Levin LR, Buck J (July 2000). "Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor". Science. 289 (5479): 625–628. Bibcode:2000Sci...289..625C. doi:10.1126/science.289.5479.625. PMID 10915626.
  • Steegborn C (December 2014). "Structure, mechanism, and regulation of soluble adenylyl cyclases - similarities and differences to transmembrane adenylyl cyclases". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1842 (12 Pt B): 2535–2547. doi:10.1016/j.bbadis.2014.08.012. PMID 25193033.

April 14, 2024
soluble, adenylyl, cyclase, also, adcy10, regulatory, cytosolic, enzyme, present, almost, every, cell, source, cyclic, adenosine, monophosphate, camp, second, messenger, that, mediates, cell, growth, differentiation, organisms, from, bacteria, higher, eukaryot. See also ADCY10 Soluble adenylyl cyclase sAC is a regulatory cytosolic enzyme present in almost every cell sAC is a source of cyclic adenosine 3 5 monophosphate cAMP a second messenger that mediates cell growth and differentiation in organisms from bacteria to higher eukaryotes sAC differentiates from the transmembrane adenylyl cyclase tmACs an important source of cAMP in that sAC is regulated by bicarbonate anions and it is dispersed throughout the cell cytoplasm sAC has been found to have various functions in physiological systems different from that of the tmACs 1 Contents 1 Genomic context and summary 2 Structure 3 Activation by bicarbonate HCO 3 and calcium Ca2 3 1 Sources of bicarbonate HCO 3 and calcium Ca2 4 Physiological effects 4 1 Brain and nervous system 4 2 Bone 4 3 Sperm 5 References 6 Further readingGenomic context and summary edit nbsp Gene Structure Chromosome 1 NC 000001 10 167778625 167883453 complement sAC is encoded in a single Homo sapiens gene identified as ADCY10 or Adenylate cyclase 10 soluble This gene packed down 33 exons that comprise greater than 100kb though it seems to utilize multiple promoters 2 3 and its mRNA undergoes extensive alternative splicing 2 3 4 5 Structure editThe functional mammalian sAC consist of two heterologous catalytic domains C1 and C2 forming the 50 kDa amino terminus of the protein 6 7 The additional 140 kDa C terminus of the enzyme includes an autoinhibitory region 8 canonical P loop potential heme binding domain 9 and leucine zipper like sequence 10 which are a form of putative regulatory domains A truncated form of the enzyme only includes the C1 and C2 domains and it is refers to as the minimal functional sAC variant 5 10 This sAC truncated form has cAMP forming activity much higher than its full length type These sAC variants are stimulated by HCO3 and respond to all known selective sAC inhibitors 6 Crystal structures of this sAC variant comprising only the catalytic core in apo form and in as complex with various substrate analogs products and regulators reveal a generic Class III AC architecture with sAC specific features 11 The structurally related domains C1 and C2 form the typical pseudo heterodimer with one active site 6 The pseudo symmetric site accommodates the sAC specific activator HCO3 which activates by triggering a rearrangement of Arg176 a residue connecting both sites The anionic sAC inhibitor 4 4 diisothiocyanatostilbene 2 2 disulfonic acid DIDS acts as a blocker for the entrance to active site and bicarbonate binding pocket 11 Activation by bicarbonate HCO 3 and calcium Ca2 editThe binding and cyclizing of adenosine 5 triphosphate ATP to the catalytic active site of the enzyme is coordinated by two metal cations The catalytic activity of sAC is increase by the presence of manganese Mn2 sAC magnesium Mg2 activity is regulated by calcium Ca2 which increases the affinity for ATP of mammalian sAC In addition bicarbonate HCO 3 releases ATP Mg2 substrate inhibition and increases Vmax of the enzyme 12 The open conformation state of sAC is reached when ATP with Ca2 bound to its g phosphate binds with specific residues in the catalytic center of the enzyme When the second metal a Mg2 ion binds to the a phosphate of ATP leads to a conformational change of the enzyme the close state The change in conformation from open to close state induces esterification of the a phosphate with the ribose in adenosine and the release of the b and g phosphates this leads to cyclizing 7 Hydrogencarbonate stimulates the enzyme s Vmax by promoting the allosteric change that leads to active site closure recruitment of the catalytic Mg2 ion and readjustment of the phosphates in the bound ATP 13 The activator bicarbonate binds to a site pseudo symmetric to the active site and triggers conformational changes by recruiting Arg176 from the active site see above structure 11 Calcium increases substrate affinity by replacing the magnesium in the ion B site which provides an anchoring point for the beta and gamma phosphates of the ATP substrate 11 13 Sources of bicarbonate HCO 3 and calcium Ca2 edit bicarbonate derived from carbonic anhydrase CA dependent hydration CO2 metabolism Enters through membrane transporting proteins or cystic fibrosis transmembrane conductance regulators Calcium enters by voltage dependent Ca2 channels or by release from the endoplasmic reticulum Hydrogencarbonate and calcium activates sAC in the nucleus sAC inside mitochondria is activated by metabolically generated CO2 through carbonic anhydrase Physiological effects editBrain and nervous system edit Astrocytes express several sAC splice variants 14 which are involved in metabolic coupling between neurons and astrocytes Increase of potassium K in the extracellular space caused by neuronal activity depolarizes the cell membrane of nearby astrocytes and facilitates the entry of hydrogencarbonate through Na HCO 3 cotransporters 7 The increase in cytosolic hydrogencarbonate activates sAC the result of this activation is the release of lactate for use as energy source by the neurons Bone edit Numerous sAC splice variants are present in osteoclast and osteoblasts 3 and mutation in the human sAC gene is associated with low spinal density 15 Calcification by osteoblasts is intrinsically related with bicarbonate and calcium Bone density experiments in mouse calvaria 16 cultured indicates that HCO 3 sensing sAC is a physiological appropriate regulator of bone formation and or reabsorption Sperm edit sAC activation by bicarbonate is necessary for motility and other aspects of capacitation in the spermatozoa of mammals 17 18 In human males mutations in the ADCY10 gene that lead to the inactivation of sAC have been linked to cases of sterility 19 Due to this essential role in male fertility sAC has been explored as a potential target for non hormonal male contraception 20 References edit Rossetti T Jackvony S Buck J Levin LR April 2021 Bicarbonate carbon dioxide and pH sensing via mammalian bicarbonate regulated soluble adenylyl cyclase Interface Focus 11 2 20200034 doi 10 1098 rsfs 2020 0034 PMC 7898154 PMID 33633833 a b Farrell J Ramos L Tresguerres M Kamenetsky M Levin LR Buck J September 2008 Somatic soluble adenylyl cyclase isoforms are unaffected in Sacy tm1Lex Sacy tm1Lex knockout mice PLOS ONE 3 9 e3251 Bibcode 2008PLoSO 3 3251F doi 10 1371 journal pone 0003251 PMC 2532759 PMID 18806876 a b c Geng W Wang Z Zhang J Reed BY Pak CY Moe OW June 2005 Cloning and characterization of the human soluble adenylyl cyclase American Journal of Physiology Cell Physiology 288 6 C1305 C1316 doi 10 1152 ajpcell 00584 2004 PMID 15659711 S2CID 1638649 Schmid A Sutto Z Nlend MC Horvath G Schmid N Buck J et al July 2007 Soluble adenylyl cyclase is localized to cilia and contributes to ciliary beat frequency regulation via production of cAMP The Journal of General Physiology 130 1 99 109 doi 10 1085 jgp 200709784 PMC 2154360 PMID 17591988 a b Jaiswal BS Conti M August 2001 Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase The Journal of Biological Chemistry 276 34 31698 31708 doi 10 1074 jbc m011698200 PMID 11423534 a b c Steegborn C December 2014 Structure mechanism and regulation of soluble adenylyl cyclases similarities and differences to transmembrane adenylyl cyclases Biochimica et Biophysica Acta BBA Molecular Basis of Disease 1842 12 Pt B 2535 2547 doi 10 1016 j bbadis 2014 08 012 PMID 25193033 a b c Tresguerres M Levin LR Buck J June 2011 Intracellular cAMP signaling by soluble adenylyl cyclase Kidney International 79 12 1277 1288 doi 10 1038 ki 2011 95 PMC 3105178 PMID 21490586 Chaloupka JA Bullock SA Iourgenko V Levin LR Buck J March 2006 Autoinhibitory regulation of soluble adenylyl cyclase Molecular Reproduction and Development 73 3 361 368 doi 10 1002 mrd 20409 PMC 3644951 PMID 16250004 Middelhaufe S Leipelt M Levin LR Buck J Steegborn C October 2012 Identification of a haem domain in human soluble adenylate cyclase Bioscience Reports 32 5 491 499 doi 10 1042 BSR20120051 PMC 3475452 PMID 22775536 a b Buck J Sinclair ML Schapal L Cann MJ Levin LR January 1999 Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals Proceedings of the National Academy of Sciences of the United States of America 96 1 79 84 Bibcode 1999PNAS 96 79B doi 10 1073 pnas 96 1 79 PMC 15096 PMID 9874775 a b c d Kleinboelting S Diaz A Moniot S van den Heuvel J Weyand M Levin LR et al March 2014 Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate Proceedings of the National Academy of Sciences of the United States of America 111 10 3727 3732 Bibcode 2014PNAS 111 3727K doi 10 1073 pnas 1322778111 PMC 3956179 PMID 24567411 Litvin TN Kamenetsky M Zarifyan A Buck J Levin LR May 2003 Kinetic properties of soluble adenylyl cyclase Synergism between calcium and bicarbonate The Journal of Biological Chemistry 278 18 15922 15926 doi 10 1074 jbc m212475200 PMID 12609998 a b Steegborn C Litvin TN Levin LR Buck J Wu H January 2005 Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment Nature Structural amp Molecular Biology 12 1 32 37 doi 10 1038 nsmb880 PMC 3644947 PMID 15619637 Choi HB Gordon GR Zhou N Tai C Rungta RL Martinez J et al September 2012 Metabolic communication between astrocytes and neurons via bicarbonate responsive soluble adenylyl cyclase Neuron 75 6 1094 1104 doi 10 1016 j neuron 2012 08 032 PMC 3630998 PMID 22998876 Reed BY Gitomer WL Heller HJ Hsu MC Lemke M Padalino P Pak CY April 2002 Identification and characterization of a gene with base substitutions associated with the absorptive hypercalciuria phenotype and low spinal bone density The Journal of Clinical Endocrinology and Metabolism 87 4 1476 1485 doi 10 1210 jcem 87 4 8300 PMID 11932268 Geng W Hill K Zerwekh JE Kohler T Muller R Moe OW August 2009 Inhibition of osteoclast formation and function by bicarbonate role of soluble adenylyl cyclase Journal of Cellular Physiology 220 2 332 340 doi 10 1002 jcp 21767 PMC 3044925 PMID 19360717 Xie F Garcia MA Carlson AE Schuh SM Babcock DF Jaiswal BS et al August 2006 Soluble adenylyl cyclase sAC is indispensable for sperm function and fertilization Developmental Biology 296 2 353 362 doi 10 1016 j ydbio 2006 05 038 PMID 16842770 Wennemuth G Carlson AE Harper AJ Babcock DF April 2003 Bicarbonate actions on flagellar and Ca2 channel responses initial events in sperm activation Development 130 7 1317 1326 doi 10 1242 dev 00353 PMID 12588848 Akbari Arvand Pipitone Giovanni Battista Anvar Zahra Jaafarinia Mojtaba Ferrari Maurizio Carrera Paola Totonchi Mehdi 4 June 2019 ADCY10 frameshift variant leading to severe recessive asthenozoospermia and segregating with absorptive hypercalciuria Human Reproduction 34 6 1155 1164 doi 10 1093 humrep dez048 PMID 31119281 Balbach Melanie Rossetti Thomas Ferreira Jacob Ghanem Lubna Ritagliati Carla Myers Robert W Huggins David J Steegborn Clemens Miranda Ileana C Meinke Peter T Buck Jochen Levin Lonny R 14 February 2023 On demand male contraception via acute inhibition of soluble adenylyl cyclase Nature Communications 14 1 637 Bibcode 2023NatCo 14 637B doi 10 1038 s41467 023 36119 6 PMC 9929232 PMID 36788210 Further reading editTresguerres M Levin LR Buck J June 2011 Intracellular cAMP signaling by soluble adenylyl cyclase Kidney International 79 12 1277 1288 doi 10 1038 ki 2011 95 PMC 3105178 PMID 21490586 Chen Y Cann MJ Litvin TN Iourgenko V Sinclair ML Levin LR Buck J July 2000 Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor Science 289 5479 625 628 Bibcode 2000Sci 289 625C doi 10 1126 science 289 5479 625 PMID 10915626 Steegborn C December 2014 Structure mechanism and regulation of soluble adenylyl cyclases similarities and differences to transmembrane adenylyl cyclases Biochimica et Biophysica Acta BBA Molecular Basis of Disease 1842 12 Pt B 2535 2547 doi 10 1016 j bbadis 2014 08 012 PMID 25193033 Retrieved from https en wikipedia org w index php title Soluble adenylyl cyclase amp oldid 1175376219, wikipedia, wiki, book, books, library,

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