The principle of solid phase DNA sequencing was described in 1989 based on binding of biotinylatedDNA to streptavidin-coated magnetic beads and elution of single DNA strands selectively using alkali.[1] The method allowed robotic applications suitable for clinical sequencing, but the magnetic handling has also found frequent use in many molecular applications, including sample handling for DNA diagnostics.[2] The use of solid phase methods for DNA handling is now frequently used as an integrated part of many of the next generation DNA sequencing methods, as well as numerous molecular diagnostics applications.[citation needed]
References
^Hultman, T; Ståhl, S; Hornes, E; Uhlén, M (11 July 1989). "Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support". Nucleic Acids Research. 17 (13): 4937–4946. doi:10.1093/nar/17.13.4937. PMC318085. PMID 2668874.
^Uhlen, M. (August 1989). "Magnetic separation of DNA". Nature. 340 (6236): 733–734. Bibcode:1989Natur.340..733U. doi:10.1038/340733a0. PMID 2770876. S2CID 4319266.
August 21, 2023
solid, phase, sequencing, principle, solid, phase, sequencing, described, 1989, based, binding, biotinylated, streptavidin, coated, magnetic, beads, elution, single, strands, selectively, using, alkali, method, allowed, robotic, applications, suitable, clinica. The principle of solid phase DNA sequencing was described in 1989 based on binding of biotinylated DNA to streptavidin coated magnetic beads and elution of single DNA strands selectively using alkali 1 The method allowed robotic applications suitable for clinical sequencing but the magnetic handling has also found frequent use in many molecular applications including sample handling for DNA diagnostics 2 The use of solid phase methods for DNA handling is now frequently used as an integrated part of many of the next generation DNA sequencing methods as well as numerous molecular diagnostics applications citation needed References Edit Hultman T Stahl S Hornes E Uhlen M 11 July 1989 Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support Nucleic Acids Research 17 13 4937 4946 doi 10 1093 nar 17 13 4937 PMC 318085 PMID 2668874 Uhlen M August 1989 Magnetic separation of DNA Nature 340 6236 733 734 Bibcode 1989Natur 340 733U doi 10 1038 340733a0 PMID 2770876 S2CID 4319266 Retrieved from https en wikipedia org w index php title Solid phase sequencing amp oldid 1150294156, wikipedia, wiki, book, books, library,
