Secretion assay is a process used in cell biology to identify cells that are secreting a particular protein (usually a cytokine). It was first developed by Manz et al. in 1995.
Usually, a cell that is secreting the protein of interest is isolated using an antibody-antibody complex that coats the cell and is able to "catch" the secreted molecules. The cell is then detected by another fluorochrome-labelled antibody, and is subsequently extracted using a process called fluorescent-activated cell sorting (FACS). The FACS method is broadly similar to the enzyme-linked immunosorbent assay (ELISA) antibody format, except that the encapsulated cells remain intact. This is advantageous as the cells are still living after the extraction has taken place.
Further advances now mean that it is possible to extract the secreting cells using a magnetic-based separation system or using a flow cytometer.
A number of commercial applications exist for secretion assay. One such example is the Gel Microdrop (GMD) technology, developed by One Cell Systems. One Cell asserts that GMD typically recovers a higher number of viable secreting cells than other methods, whilst ignoring any cells which are not secreting the desired protein.
Referencesedit
Manz, Rudolf; et al. (March 1995). "Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix". Proceedings of the National Academy of Sciences. 92 (6): 1921–1925. Bibcode:1995PNAS...92.1921M. doi:10.1073/pnas.92.6.1921. PMC42394. PMID 7892200.
Further readingedit
Turcanu, Victor; Williams, Neil A. (2001). "Cell identification and isolation on the basis of cytokine secretion: A novel tool for investigating immune responses". Nature Medicine. 7 (3): 373–376. doi:10.1038/85533. PMID 11231640. S2CID 837840.
External linksedit
One Cell Systems - Gel Microdrop (GMD) Secretion Assay technology.
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secretion, assay, process, used, cell, biology, identify, cells, that, secreting, particular, protein, usually, cytokine, first, developed, manz, 1995, usually, cell, that, secreting, protein, interest, isolated, using, antibody, antibody, complex, that, coats. Secretion assay is a process used in cell biology to identify cells that are secreting a particular protein usually a cytokine It was first developed by Manz et al in 1995 Usually a cell that is secreting the protein of interest is isolated using an antibody antibody complex that coats the cell and is able to catch the secreted molecules The cell is then detected by another fluorochrome labelled antibody and is subsequently extracted using a process called fluorescent activated cell sorting FACS The FACS method is broadly similar to the enzyme linked immunosorbent assay ELISA antibody format except that the encapsulated cells remain intact This is advantageous as the cells are still living after the extraction has taken place Further advances now mean that it is possible to extract the secreting cells using a magnetic based separation system or using a flow cytometer A number of commercial applications exist for secretion assay One such example is the Gel Microdrop GMD technology developed by One Cell Systems One Cell asserts that GMD typically recovers a higher number of viable secreting cells than other methods whilst ignoring any cells which are not secreting the desired protein References editManz Rudolf et al March 1995 Analysis and sorting of live cells according to secreted molecules relocated to a cell surface affinity matrix Proceedings of the National Academy of Sciences 92 6 1921 1925 Bibcode 1995PNAS 92 1921M doi 10 1073 pnas 92 6 1921 PMC 42394 PMID 7892200 Further reading editTurcanu Victor Williams Neil A 2001 Cell identification and isolation on the basis of cytokine secretion A novel tool for investigating immune responses Nature Medicine 7 3 373 376 doi 10 1038 85533 PMID 11231640 S2CID 837840 External links editOne Cell Systems Gel Microdrop GMD Secretion Assay technology nbsp This cell biology article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Secretion assay amp oldid 1192016171, wikipedia, wiki, book, books, library,
