pSC101 is a DNAplasmid that is used as a cloningvector in genetic cloning experiments. pSC101 was the first cloning vector, used in 1973 by Herbert Boyer and Stanley Norman Cohen. Using this plasmid they have demonstrated that a gene from a frog could be transferred into bacterial cells and then expressed by the bacterial cells. The plasmid is a natural plasmid from Salmonella typhimurium.[1]
Historyedit
In the early 1970s,[2]Herbert Boyer and Stanley Norman Cohen produced pSC101, the first plasmid vector for cloning purposes. Soon after successfully cloning two pSC101 plasmids together to create one large plasmid, they published the results describing the experiment, in 1973.[2] The cloning of genes into plasmids occurred soon after. In 1980,[2] pSC101 became the first patented commercial DNA cloning vector when patents were awarded to Boyer and Cohen. The "SC" stands for Stanley Cohen. Although the original pSC101 only contained tetracycline resistance and a restriction site for EcoRI, the commercially available pSC101 gained restriction sites for several enzymes, including HindIII, in addition to the EcoRI site.
Referencesedit
^Manen D.; Caro L. (February 1991). "The replication of plasmid pSC101". Mol. Microbiol. 5 (2): 233–7. doi:10.1111/j.1365-2958.1991.tb02103.x. PMID 2041467. S2CID 37314534.
^ abcThieman, W.J. & Palladino, M.A. (2004). Introduction to Biotechnology. Pearson Education, Benjamin Cummings. p. 55.
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psc101, plasmid, that, used, cloning, vector, genetic, cloning, experiments, first, cloning, vector, used, 1973, herbert, boyer, stanley, norman, cohen, using, this, plasmid, they, have, demonstrated, that, gene, from, frog, could, transferred, into, bacterial. pSC101 is a DNA plasmid that is used as a cloning vector in genetic cloning experiments pSC101 was the first cloning vector used in 1973 by Herbert Boyer and Stanley Norman Cohen Using this plasmid they have demonstrated that a gene from a frog could be transferred into bacterial cells and then expressed by the bacterial cells The plasmid is a natural plasmid from Salmonella typhimurium 1 History editIn the early 1970s 2 Herbert Boyer and Stanley Norman Cohen produced pSC101 the first plasmid vector for cloning purposes Soon after successfully cloning two pSC101 plasmids together to create one large plasmid they published the results describing the experiment in 1973 2 The cloning of genes into plasmids occurred soon after In 1980 2 pSC101 became the first patented commercial DNA cloning vector when patents were awarded to Boyer and Cohen The SC stands for Stanley Cohen Although the original pSC101 only contained tetracycline resistance and a restriction site for EcoRI the commercially available pSC101 gained restriction sites for several enzymes including HindIII in addition to the EcoRI site References edit Manen D Caro L February 1991 The replication of plasmid pSC101 Mol Microbiol 5 2 233 7 doi 10 1111 j 1365 2958 1991 tb02103 x PMID 2041467 S2CID 37314534 a b c Thieman W J amp Palladino M A 2004 Introduction to Biotechnology Pearson Education Benjamin Cummings p 55 nbsp This genetics article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title PSC101 amp oldid 1177613896, wikipedia, wiki, book, books, library,
