Myosin light chain phosphatase (MLCP) consists of three subunits- catalytic subunit, large subunit/myosin binding subunit (MBS) and small subunit (sm-M20). This gene is a multi-functional gene which encodes both MBS and sm-M20. MLCP regulates myosins and the dephosphorylation is enhanced by the presence of MBS. The sm-M20 is suggested to play a regulatory role in muscle contraction by binding to MBS. MBS is also encoded by another gene, myosin light chain phosphatase target subunit 1. sm-M20 shows higher binding affinity to this gene product than to myosin light chain phosphatase target subunit 2-MBS even though the two MBS proteins are highly similar. Although both MBSs increase the activity of MLCP, myosin light chain phosphatase target subunit 1-MBS is a more efficient activator. There are four alternatively spliced transcript variants described; two alter the MBS coding region and two alter the sm-M20 coding region of this gene.[4]
^ abcGRCh38: Ensembl release 89: ENSG00000077157 - Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Fujioka M, Takahashi N, Odai H, Araki S, Ichikawa K, Feng J, Nakamura M, Kaibuchi K, Hartshorne DJ, Nakano T, Ito M (Jun 1998). "A new isoform of human myosin phosphatase targeting/regulatory subunit (MYPT2): cDNA cloning, tissue expression, and chromosomal mapping". Genomics. 49 (1): 59–68. doi:10.1006/geno.1998.5222. PMID 9570949.
^Bannert, Norbert; Vollhardt Karin; Asomuddinov Bakhtier; Haag Marion; König Herbert; Norley Stephen; Kurth Reinhard (Oct 2003). "PDZ Domain-mediated interaction of interleukin-16 precursor proteins with myosin phosphatase targeting subunits". J. Biol. Chem. United States. 278 (43): 42190–9. doi:10.1074/jbc.M306669200. ISSN 0021-9258. PMID 12923170.
Further readingedit
Somlyo AP, Wu X, Walker LA, Somlyo AV (1999). "Pharmacomechanical coupling: the role of calcium, G-proteins, kinases and phosphatases". Rev. Physiol. Biochem. Pharmacol. 134: 201–34. doi:10.1007/3-540-64753-8_5. ISBN978-3-540-64753-9. PMID 10087910.
Seki N, Ohira M, Nagase T, et al. (1998). "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain". DNA Res. 4 (5): 345–9. doi:10.1093/dnares/4.5.345. PMID 9455484.
Moorhead G, Johnson D, Morrice N, Cohen P (1998). "The major myosin phosphatase in skeletal muscle is a complex between the beta-isoform of protein phosphatase 1 and the MYPT2 gene product". FEBS Lett. 438 (3): 141–4. doi:10.1016/S0014-5793(98)01276-9. PMID 9827534. S2CID 35052936.
Somlyo AP (1999). "Kinases, myosin phosphatase and Rho proteins: curiouser and curiouser". J. Physiol. 516 (3): 630. doi:10.1111/j.1469-7793.1999.0630u.x. PMC2269296. PMID 10200412.
Arimura T, Suematsu N, Zhou YB, et al. (2001). "Identification, characterization, and functional analysis of heart-specific myosin light chain phosphatase small subunit". J. Biol. Chem. 276 (9): 6073–82. doi:10.1074/jbc.M008566200. PMID 11067852.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC139241. PMID 12477932.
Bannert N, Vollhardt K, Asomuddinov B, et al. (2004). "PDZ Domain-mediated interaction of interleukin-16 precursor proteins with myosin phosphatase targeting subunits". J. Biol. Chem. 278 (43): 42190–9. doi:10.1074/jbc.M306669200. PMID 12923170.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC528928. PMID 15489334.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Okamoto R, Kato T, Mizoguchi A, et al. (2007). "Characterization and function of MYPT2, a target subunit of myosin phosphatase in heart". Cell. Signal. 18 (9): 1408–16. doi:10.1016/j.cellsig.2005.11.001. PMID 16431080.
Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.
This article on a gene on human chromosome 1 is a stub. You can help Wikipedia by expanding it.
ppp1r12b, protein, phosphatase, regulatory, subunit, enzyme, that, humans, encoded, gene, identifiersaliases, mypt2, pp1bp55, protein, phosphatase, regulatory, subunit, 12bexternal, idsomim, 603768, homologene, 136757, genecards, gene, location, human, chromos. Protein phosphatase 1 regulatory subunit 12B is an enzyme that in humans is encoded by the PPP1R12B gene 3 4 PPP1R12BIdentifiersAliasesPPP1R12B MYPT2 PP1bp55 protein phosphatase 1 regulatory subunit 12BExternal IDsOMIM 603768 HomoloGene 136757 GeneCards PPP1R12BGene location Human Chr Chromosome 1 human 1 Band1q32 1Start202 348 699 bp 1 End202 592 706 bp 1 RNA expression patternBgeeHumanMouse ortholog Top expressed insaphenous veinright coronary arteryright ventriclepopliteal arterythoracic aortaascending aortagastric mucosaseminal vesiculagallbladdermyometriumn aMore reference expression dataBioGPSMore reference expression dataGene ontologyMolecular functionenzyme activator activity protein binding protein kinase binding phosphatase regulator activity enzyme inhibitor activityCellular componentcytoplasm cytosol A band Z disc nucleoplasm cytoskeletonBiological processregulation of muscle contraction positive regulation of catalytic activity signal transduction regulation of catalytic activity negative regulation of catalytic activity G2 M transition of mitotic cell cycleSources Amigo QuickGOOrthologsSpeciesHumanMouseEntrez4660n aEnsemblENSG00000077157n aUniProtO60237n aRefSeq mRNA NM 001167857NM 001167858NM 001197131NM 002481NM 032103NM 032104NM 032105NM 001331029n aRefSeq protein NP 001161329NP 001161330NP 001184060NP 001317958NP 002472NP 115286NP 115287n aLocation UCSC Chr 1 202 35 202 59 Mbn aPubMed search 2 n aWikidataView Edit HumanMyosin light chain phosphatase MLCP consists of three subunits catalytic subunit large subunit myosin binding subunit MBS and small subunit sm M20 This gene is a multi functional gene which encodes both MBS and sm M20 MLCP regulates myosins and the dephosphorylation is enhanced by the presence of MBS The sm M20 is suggested to play a regulatory role in muscle contraction by binding to MBS MBS is also encoded by another gene myosin light chain phosphatase target subunit 1 sm M20 shows higher binding affinity to this gene product than to myosin light chain phosphatase target subunit 2 MBS even though the two MBS proteins are highly similar Although both MBSs increase the activity of MLCP myosin light chain phosphatase target subunit 1 MBS is a more efficient activator There are four alternatively spliced transcript variants described two alter the MBS coding region and two alter the sm M20 coding region of this gene 4 Interactions editPPP1R12B has been shown to interact with Interleukin 16 5 References edit a b c GRCh38 Ensembl release 89 ENSG00000077157 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Fujioka M Takahashi N Odai H Araki S Ichikawa K Feng J Nakamura M Kaibuchi K Hartshorne DJ Nakano T Ito M Jun 1998 A new isoform of human myosin phosphatase targeting regulatory subunit MYPT2 cDNA cloning tissue expression and chromosomal mapping Genomics 49 1 59 68 doi 10 1006 geno 1998 5222 PMID 9570949 a b Entrez Gene PPP1R12B protein phosphatase 1 regulatory inhibitor subunit 12B Bannert Norbert Vollhardt Karin Asomuddinov Bakhtier Haag Marion Konig Herbert Norley Stephen Kurth Reinhard Oct 2003 PDZ Domain mediated interaction of interleukin 16 precursor proteins with myosin phosphatase targeting subunits J Biol Chem United States 278 43 42190 9 doi 10 1074 jbc M306669200 ISSN 0021 9258 PMID 12923170 Further reading editSomlyo AP Wu X Walker LA Somlyo AV 1999 Pharmacomechanical coupling the role of calcium G proteins kinases and phosphatases Rev Physiol Biochem Pharmacol 134 201 34 doi 10 1007 3 540 64753 8 5 ISBN 978 3 540 64753 9 PMID 10087910 Seki N Ohira M Nagase T et al 1998 Characterization of cDNA clones in size fractionated cDNA libraries from human brain DNA Res 4 5 345 9 doi 10 1093 dnares 4 5 345 PMID 9455484 Moorhead G Johnson D Morrice N Cohen P 1998 The major myosin phosphatase in skeletal muscle is a complex between the beta isoform of protein phosphatase 1 and the MYPT2 gene product FEBS Lett 438 3 141 4 doi 10 1016 S0014 5793 98 01276 9 PMID 9827534 S2CID 35052936 Somlyo AP 1999 Kinases myosin phosphatase and Rho proteins curiouser and curiouser J Physiol 516 3 630 doi 10 1111 j 1469 7793 1999 0630u x PMC 2269296 PMID 10200412 Arimura T Suematsu N Zhou YB et al 2001 Identification characterization and functional analysis of heart specific myosin light chain phosphatase small subunit J Biol Chem 276 9 6073 82 doi 10 1074 jbc M008566200 PMID 11067852 Strausberg RL Feingold EA Grouse LH et al 2003 Generation and initial analysis of more than 15 000 full length human and mouse cDNA sequences Proc Natl Acad Sci U S A 99 26 16899 903 Bibcode 2002PNAS 9916899M doi 10 1073 pnas 242603899 PMC 139241 PMID 12477932 Bannert N Vollhardt K Asomuddinov B et al 2004 PDZ Domain mediated interaction of interleukin 16 precursor proteins with myosin phosphatase targeting subunits J Biol Chem 278 43 42190 9 doi 10 1074 jbc M306669200 PMID 12923170 Gerhard DS Wagner L Feingold EA et al 2004 The Status Quality and Expansion of the NIH Full Length cDNA Project The Mammalian Gene Collection MGC Genome Res 14 10B 2121 7 doi 10 1101 gr 2596504 PMC 528928 PMID 15489334 Rual JF Venkatesan K Hao T et al 2005 Towards a proteome scale map of the human protein protein interaction network Nature 437 7062 1173 8 Bibcode 2005Natur 437 1173R doi 10 1038 nature04209 PMID 16189514 S2CID 4427026 Okamoto R Kato T Mizoguchi A et al 2007 Characterization and function of MYPT2 a target subunit of myosin phosphatase in heart Cell Signal 18 9 1408 16 doi 10 1016 j cellsig 2005 11 001 PMID 16431080 Gregory SG Barlow KF McLay KE et al 2006 The DNA sequence and biological annotation of human chromosome 1 Nature 441 7091 315 21 Bibcode 2006Natur 441 315G doi 10 1038 nature04727 PMID 16710414 nbsp This article on a gene on human chromosome 1 is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title PPP1R12B amp oldid 1183993143, wikipedia, wiki, book, books, library,
